Parentage Assignment Using Microsatellites Reveals Multiple Mating in Aedes aegypti (Diptera: Culicidae): Implications for Mating Dynamics.

The mosquito Aedes aegypti is the primary vector of the dengue, yellow fever, and chikungunya viruses. Evidence shows that Ae. aegypti males are polyandrous whereas Ae. aegypti females are monandrous in mating. However, the degree to which Ae. aegypti males and females can mate with different partners has not been rigorously tested. Therefore, this study examined the rates of polyandry via parentage assignment in three sets of competitive mating experiments using wild-type male and female Ae. aegypti. Parentage assignment was monitored using nine microsatellite DNA markers. All Ae. aegypti offspring were successfully assigned to parents with 80% or 95% confidence using CERVUS software. The results showed that both male and female Ae. aegypti mated with up to 3-4 different partners. Adults contributed differentially to the emergent offspring, with reproductive outputs ranging from 1 to 25 viable progeny. This study demonstrates a new perspective on the capabilities of male and female Ae. aegypti in mating. These findings are significant because successful deployment of reproductive control methods using genetic modification or sterile Ae. aegypti must consider the following criteria regarding their mating fitness: 1) choosing Ae. aegypti males that can mate with many different females; 2) testing how transformed Ae. aegypti male perform with polyandrous females; and 3) prioritizing the selection of polyandrous males and/or females Ae. aegypti that have the most offspring.

Understanding Social Dimensions in Wildlife Conservation: Multiple Stakeholder Views.

Numerous studies show the importance of social understanding in addressing multifaceted conservation issues. Building on a conservation planning framework, this study examines the social dimensions of wildlife conservation in Kinabatangan, Sabah, Malaysia. It employs a qualitative approach by conducting in-depth, semi-structured interviews with sixty informants drawn from local community members, government officials, tourism operators, non-government organizations, and the private sector. Our results show that the incidence of human-wildlife conflicts has reduced in the region, but that conflicts among stakeholders themselves about wildlife still remain a significant threat for attaining successful conservation outcomes. Further stakeholder perceptions of increased wildlife numbers often contrast with actual counts returned by periodical surveys conducted by conservation agencies, e.g., showing a 30% decline of orangutans and a 29% decline of gibbon abundance. This shows that evidence-based conservation messages have not been communicated well. The study has implications for enhancing social values among conservation players, promoting local community empowerment and revising conservation awareness programs.