Liquid Chromatographic Determination of Biogenic Amines in Fish Based on Pyrene Sulfonyl Chloride Pre-Column Derivatization.

Monitoring of biogenic amines in food is important for quality control, in terms of freshness evaluation and even more for food safety. A novel and cost-effective method was developed and validated for the determination of the main biogenic amines: histamine, putrescine, cadaverine, spermidine and spermine in fish tissues. The method includes extraction of amines with perchloric acid, pre-column derivatization with Pyrene Sulfonyl Chloride (PSCl), extraction of derivatives with toluene, back-dissolution in ACN after evaporation and determination by reversed phase high performance liquid chromatography with UV and intramolecular excimer fluorescence detection. The structure of the pyrene-derivatives was confirmed by liquid chromatography-mass spectrometry with electrospray ionization. The standard addition technique was applied for the quantitation due to significant matrix effect, while the use of 1,7-diaminoheptane as internal standard offered an additional confirmation tool for the identification of the analytes. Method repeatability expressed as %RSD ranged between 7.4-14% for the different amines and recovery ranged from 67% for histamine up to 114% for spermine. The limits of detection ranged between 0.1-1.4 mg kg-1 and the limits of quantification between 0.3-4.2 mg kg-1. The method was applied to canned fish samples and the concentrations of the individual biogenic amines were below the detection limit up to 40.1 mg kg-1, while their sum was within the range 4.1-49.6 mg kg-1.

Dataset on the imprint of the Agia Zoni II tanker oil spill on the marine ecosystem of Saronikos Gulf.

These data relate to the research article entitled "Imprint and short-term fate of the Agia Zoni II tanker oil spill on the marine ecosystem of Saronikos Gulf" by Parinos et al., 2019 [1]. The dataset includes the concentrations of 32 individual compounds/groups of polycyclic aromatic hydrocarbons (PAHs) determined in 235 seawater samples and 55 sediment samples analyzed during the monitoring survey conducted by the Hellenic Centre for Marine Research (H.C.M.R.) following the September 2017 Agia Zoni II heavy fuel oil (HFO) spill incident in Saronikos Gulf, Greece. The survey effort included 69 seawater sampling sites, of which 55 coastal and 14 open sea areas, and 22 sediment sampling sites across the inner Saronikos Gulf, aiming to assess the spatial and temporal imprint of the spilled oil during the first six months from the incident. The data were acquired by means of gas chromatography - mass spectrometry, following proper pre-treatment of the collected samples. This dataset is, to the best of our knowledge, the very first PAHs record related to the Agia Zoni II oil spill incident, that should be of interest for future scientific research on this and HFO spills in general.