RESUMO
A membrane bioreactor (MBR) and a hybrid moving bed biofilm reactor-membrane bioreactor (hybrid MBBR-MBR) for municipal wastewater treatment were studied to determine the effect of salinity on nitrogen removal and autotrophic kinetics. The biological systems were analyzed during the start-up phase with a hydraulic retention time (HRT) of 6 h, total biomass concentration of 2,500 mg L-1 in the steady state, and electric conductivities of 1.05 mS cm-1 for MBR and hybrid MBBR-MBR working under regular salinity and conductivity variations of 1.2-6.5 mS cm-1 for MBR and hybrid MBBR-MBR operating at variable salinity. The variable salinity affected the autotrophic biomass, which caused a reduction of the nitrogen degradation rate, an increase of time to remove ammonium from municipal wastewater and longer duration of the start-up phase for the MBR and hybrid MBBR-MBR.
Assuntos
Reatores Biológicos , Salinidade , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/metabolismo , Compostos de Amônio/metabolismo , Biofilmes , Biomassa , Condutividade Elétrica , Cinética , Membranas Artificiais , Nitrogênio/metabolismo , Águas ResiduáriasRESUMO
The start-up phase of a membrane bioreactor (MBR) for municipal wastewater treatment was studied to determine the effect of temperature on the organic matter removal and heterotrophic kinetics. The MBR system was analyzed during four start-up phases with values of hydraulic retention time (HRT) of 6 h and 10 h, mixed liquor suspended solids (MLSS) concentrations of 4,000 mg L-1 and 7,000 mg L-1 in the steady state, and temperature values of 11.5, 14.2, 22.9 and 30.1 °C. The influence of temperature on the biological process of organic matter removal was determined through the Arrhenius equation and Monod model. At the most favorable operation conditions of HRT (10 h) and MLSS (7,000 mg L-1) corresponding to phase 4, the effect of these variables dominated over the temperature. Heterotrophic biomass from phase 2 (HRT = 10 h, MLSS = 4,000 mg L-1 and T = 30.1 °C) had the highest values of chemical oxygen demand (COD) degradation rate (rsu,H), implying less time to remove organic matter and shorter duration of the start-up phase.
Assuntos
Reatores Biológicos/microbiologia , Membranas Artificiais , Modelos Teóricos , Temperatura , Purificação da Água/métodos , Análise da Demanda Biológica de Oxigênio , Biomassa , Cinética , Esgotos/química , Águas Residuárias/químicaRESUMO
The moving bed biofilm reactor-membrane bioreactor (MBBR-MBR) is a novel solution to conventional activated sludge processes and membrane bioreactors. In this study, a pure MBBR-MBR was studied. The pure MBBR-MBR mainly had attached biomass. The bioreactor operated with a hydraulic retention time (HRT) of 9.5 h. The kinetic parameters for heterotrophic and autotrophic biomasses, mainly nitrite-oxidizing bacteria (NOB), were evaluated. The analysis of the bacterial community structure of the ammonium-oxidizing bacteria (AOB), NOB, and denitrifying bacteria (DeNB) from the pure MBBR-MBR was carried out by means of pyrosequencing to detect and quantify the contribution of the nitrifying and denitrifying bacteria in the total bacterial community. The relative abundance of AOB, NOB, and DeNB were 5, 1, and 3%, respectively, in the mixed liquor suspended solids (MLSS), and these percentages were 18, 5, and 2%, respectively, in the biofilm density (BD) attached to carriers. The pure MBBR-MBR had a high efficiency of total nitrogen (TN) removal of 71.81±16.04%, which could reside in the different bacterial assemblages in the fixed biofilm on the carriers. In this regard, the kinetic parameters for autotrophic biomass had values of YA=2.3465 mg O2 mg N(-1), µm, A=0.7169 h(-1), and KNH=2.0748 mg NL(-1).
Assuntos
Bactérias/metabolismo , Biofilmes/crescimento & desenvolvimento , Reatores Biológicos/microbiologia , Biota , Membranas/microbiologia , Nitrificação , Águas Residuárias/microbiologia , Compostos de Amônio/metabolismo , Bactérias/classificação , Metagenômica , Nitritos/metabolismo , Oxirredução , Purificação da ÁguaRESUMO
A bench-scale pure moving bed bioreactor-membrane bioreactor (MBBR-MBR) used for the treatment of urban wastewater was analyzed for the identification of bacterial strains with the potential capacity for calcium carbonate and struvite biomineral formation. Isolation of mineral-forming strains on calcium carbonate and struvite media revealed six major colonies with a carbonate or struvite precipitation capacity in the biofouling on the membrane surface and showed that heterotrophic bacteria with the ability to precipitate calcium carbonate and struvite constituted ~7.5% of the total platable bacteria. These belonged to the genera Lysinibacillus, Trichococcus, Comamomas and Bacillus. Pyrosequencing analysis of the microbial communities in the suspended cells and membrane biofouling showed a high degree of similarity in all the samples collected with respect to bacterial assemblage. The study of operational taxonomic units (OTUs) identified through pyrosequencing suggested that ~21% of the total bacterial community identified in the biofouling could potentially form calcium carbonate or struvite crystals in the pure MBBR-MBR system used for the treatment of urban wastewater.
Assuntos
Bactérias/classificação , Incrustação Biológica , Reatores Biológicos/microbiologia , Carbonato de Cálcio/química , Compostos de Magnésio/química , Metagenômica , Fosfatos/química , Bactérias/genética , Bactérias/isolamento & purificação , Biofilmes/crescimento & desenvolvimento , Membranas Artificiais , Estruvita , Águas Residuárias/química , Águas Residuárias/microbiologiaRESUMO
A hybrid moving bed biofilm reactor-membrane bioreactor (hybrid MBBR-MBR) system was studied as an alternative solution to conventional activated sludge processes and membrane bioreactors. This paper shows the results obtained from three laboratory-scale wastewater treatment plants working in parallel in the start-up and steady states. The first wastewater treatment plant was a MBR, the second one was a hybrid MBBR-MBR system containing carriers both in anoxic and aerobic zones of the bioreactor (hybrid MBBR-MBRa), and the last one was a hybrid MBBR-MBR system which contained carriers only in the aerobic zone (hybrid MBBR-MBRb). The reactors operated with a hydraulic retention time of 30.40 h. A kinetic study for characterizing heterotrophic biomass was carried out and organic matter and nutrients removals were evaluated. The heterotrophic biomass of the hybrid MBBR-MBRb showed the best kinetic performance in the steady state, with yield coefficient for heterotrophic biomass=0.30246 mg volatile suspended solids per mg chemical oxygen demand, maximum specific growth rate for heterotrophic biomass=0.00308 h(-1) and half-saturation coefficient for organic matter=3.54908 mg O2 L(-1). The removal of organic matter was supported by the kinetic study of heterotrophic biomass.
Assuntos
Bactérias/metabolismo , Biofilmes , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/microbiologia , Bactérias/química , Biodegradação Ambiental , Biomassa , Reatores Biológicos/microbiologia , Cinética , Esgotos/química , Esgotos/microbiologia , Águas Residuárias/químicaRESUMO
Performance of a bench-scale wastewater treatment plant, which consisted of a membrane bioreactor, was monitored daily using pure oxygen and air to supply aerobic conditions with the aim of studying the increases of the aeration and sludge removal efficiencies and the effect of the temperature. The results showed the capacity of membrane bioreactor systems for removing organic matter. The alpha-factors of the aeration were determined for six different MLSS concentrations in order to understand the system working when pure oxygen and air were used to supply aerobic conditions in the system. Aeration efficiency was increased between 30.7 and 45.9% when pure oxygen was used in the operation conditions (a hydraulic retention time of 12 h and MLSS concentrations between 4,018 and 11,192 mg/L). Sludge removal efficiency increased incrementally, from 0.2 to 1.5% when pure oxygen was used at low sludge retention time and from 1.5% to 15.4% at medium sludge retention time when temperature conditions were lower than 20°C. Moreover, the difference between calculated and experimental sludge retention time was lesser when pure oxygen was used to provide aerobic conditions, so the influence of the temperature decreased when the pure oxygen was used. These results showed the convenience of using pure oxygen due to the improvement in the performance of the system.
Assuntos
Ar , Reatores Biológicos/microbiologia , Oxigênio/metabolismo , Esgotos/microbiologia , Temperatura , Aerobiose , TempoRESUMO
A pilot-scale ultrafiltration membrane bioreactor (MBR) was used for the aerobic treatment of urban wastewater in four experimental stages influenced by seasonal temperature and different sets of operation conditions. The structure of the ammonia-oxidizing bacteria (AOB) community was profiled by temperature gradient gel electrophoresis (TGGE), based on the amplification and separation of partial ammonia-monoxygenase subunit A (amoA) genes. Canonical correspondence analysis revealed that temperature, hydraulic retention time and percentage of ammonia removal had a significant effect on the fingerprints of AOB communities. Phylogenetic analysis conducted on amoA/AmoA sequences of reamplified TGGE bands showed, however, that closely related ammonia-oxidizing populations inhabited the sludge of the MBR in all experimental stages. Nitrosomonas cluster 7 populations (N. europaea-N. eutropha cluster) prevailed under all conditions tested, even when the MBR was operated under complete biomass retention or at low temperatures, suggesting that the high ammonia concentrations in the system were determinant to select r-strategist AOB.
Assuntos
Amônia/metabolismo , Reatores Biológicos , Membranas Artificiais , Nitrosomonas/metabolismo , Águas Residuárias , Poluentes Químicos da Água/metabolismo , Sequência de Bases , Primers do DNA , Eletroforese em Gel de Gradiente Desnaturante , Oxirredução , Reação em Cadeia da Polimerase , UrbanizaçãoRESUMO
At present, there is great concern about limited water resources and water quality, which require a more advanced technology. The Moving Bed Biofilm Reactor (MBBR) has been shown to be an efficient technology for removal of organic matter and nutrients in industrial and urban wastewater treatment. However, there are some pollutants which are more difficult to remove by biological processes, so this process can be improved with additional physical and chemical treatments such as electrocoagulation, which appears to be a promising technology in electrochemical treatments. In this research, urban wastewater was treated in an MBBR plant with an electrocoagulation pre-treatment. K1 from AnoxKaldnes and AQWISE ABC5 from Aqwise were the carriers studied under three different filling ratios (20, 35, and 50%). The experimental pilot plant had four bioreactors with 20 L of operation volume and a common feed tank with 100 L of operation volume. The movement of the carriers was generated by aeration and stirrer systems. Organic matter removal was studied by analysis of soluble chemical oxygen demand (sCOD). The maximum organic matter removal in this MBBR system was 65.8% ± 1.4% and 78.4% ± 0.1% for K1 and Aqwise ABC5 carriers, respectively. Moreover, the bacterial diversity of the biofilm was studied by temperature-gradient gel electrophoresis (TGGE) of PCR-amplified partial 16S rRNA genes. 20 prominent TGGE bands were successfully reamplified and sequenced, being the predominant population: ß-Proteobacteria, α-Proteobacteria, and Actinobacteria.
Assuntos
Biofilmes , Reatores Biológicos/microbiologia , Eletrocoagulação/métodos , Eliminação de Resíduos Líquidos/métodosRESUMO
Because of the growing need to eliminate undesirable microorganisms in different industrial treatments, mainly in the food and agricultural sector and the pharmaceutical industry, a number of increasingly effective systems for disinfection to eliminate microorganisms have been devised. This article analyzes different methods to eliminate and/or significantly reduce the number of microorganisms in industrial contexts and in environmental engineering. Although, in the past, thermal treatments had been used most frequently for microbial elimination, the method is costly and has the disadvantage of modifying the organoleptic and/or physicochemical properties of the food products. For this reason, new technologies rapidly are being developed, such as high-intensity pulsed electric fields, high-pressure systems, ultrasounds, and irradiation, which effectively eliminate microorganisms without deteriorating the properties of the product. These emerging technologies are potentially applicable in the field of environmental engineering.
Assuntos
Microbiologia Industrial/métodos , Eliminação de Resíduos Líquidos/métodos , Desinfecção/métodos , Poluentes Químicos da Água/metabolismoRESUMO
Pure oxygen to supply the aerobic condition was used in the performance of a bench-scale submerged membrane bioreactor (MBR). The pilot plant was located in the wastewater treatment plant of the city of Granada (Spain) and the experimental work was divided into two stages (Unsteady state and steady state conditions). Operation parameters (MLSS, MLVSS and dissolved oxygen concentration) and physical characteristics (temperature, conductivity, pH, COD and BOD(5)) were daily monitored. The results showed the capacity of the MBR systems to remove organic material under a hydraulic retention time of 18.46 h and sludge retention time of 18.6 days. Therefore, Viscosity of the sludge and alphakLa-factor of the aeration, were determinate in the steady stage condition to understand the behavior of the system when pure oxygen has been used to supply the aerobic conditions of the MBR system showed an alpha-factor of 0.238 when the viscosity of the system was 4.04 Cp.
Assuntos
Reatores Biológicos/microbiologia , Modelos Biológicos , Consumo de Oxigênio/fisiologia , Oxigênio/metabolismo , Esgotos/microbiologia , Simulação por Computador , Desenho de Equipamento , Análise de Falha de Equipamento , Projetos Piloto , ViscosidadeRESUMO
This research was performed to assess the production of reclaimed water from urban wastewater in membrane bioreactor - advanced oxidation process (MBR-AOP) and moving bed biofilm reactor - membrane bioreactor - advanced oxidation process (MBBR-MBR-AOP) combined treatments to study the effect of biofilm incorporation. Both combined treatments were operated at the same conditions (10 h of hydraulic retention time, 6500 mg/L of mixed liquor suspended solids and 25 mg/L of hydrogen peroxide dosage over 15 min). Additionally, the removal capacity of some pharmaceuticals (carbamazepine, ciprofloxacin and ibuprofen) and their impact on the kinetic behaviour of the biomass in both systems were evaluated. From the results, it was found a membrane-based bioreactor can achieve both wastewater secondary treatment and pre-treatment for advanced oxidation process, so both MBR-AOP and MBBR-MBR-AOP treatments have a great potential to produce high quality reclaimed water (biological oxygen demand <0.5 mgO2/L, suspended solids <1 mg/L, turbidity <1 NTU and no presence of E. coli), according to European Commission proposal 2018/0169/COD. The addition of carriers improved the biodegradation of the most persistent pharmaceuticals in the biological treatment (from 69.20 ± 1.54% to 75.14 ± 2.71% for carbamazepine and from 60.41 ± 2.16 to 63.14 ± 2.70% for ciprofloxacin). It had, as a consequence, the MBBR-MBR-AOP system showing a complete degradation of pharmaceuticals after 5 min AOP treatment compared to the MBR-AOP system. The loss of biomass in the MBR-AOP (from 5233.45 to 4451.92 mg/L) and the increase of the substrate degradation rate for organic matter in both treatments (from 37.27 to 41.42 and from 30.25 to 33.19 mgO2/(L·h) in MBR-AOP and MBBR-MBR-AOP, respectively) are some of the consequences of pharmaceuticals in urban wastewater.
Assuntos
Reatores Biológicos , Purificação da Água , Biofilmes , Escherichia coli , Membranas Artificiais , Eliminação de Resíduos Líquidos , Águas Residuárias , Qualidade da ÁguaRESUMO
Over the last years, an increasing concern has emerged regarding the eco-friendly management of wastewater. Apart from the role of wastewater treatment plants (WWTPs) for wastewater and sewage sludge treatment, the increasing need of the recovery of the resources contained in wastewater, such as nutrients and water, should be highlighted. This would allow for transforming a wastewater treatment plant (WWTP) into a sustainable technological system. The objective of this review is to propose a moving bed biofilm reactor (MBBR) as a novel technology that contributes to the circularity of the wastewater treatment sector according to the principles of circular economy. In this regard, this paper aims to consider the MBBR process as the initial step for water reuse, and nutrient removal and recovery, within the circular economy model.
Assuntos
Biofilmes , Águas Residuárias , Reatores Biológicos , Nutrientes , Esgotos , Eliminação de Resíduos LíquidosRESUMO
Bacteria are key players in biological wastewater treatments (WWTs), thus a firm knowledge of the bacterial population dynamics is crucial to understand environmental/operational factors affecting the efficiency and stability of the biological depuration process. Unfortunately, little is known about the microbial ecology of the advanced biological WWTs combining suspended biomass (SB) and attached biofilms (AB). This study explored in depth the bacterial community structure and population dynamics in each biomass fraction from a pilot-scale moving bed membrane bioreactor (MBMBR) treating municipal sewage, by means of temperature-gradient gel electrophoresis (TGGE) and 454-pyrosequencing. Eight experimental phases were conducted, combining different carrier filling ratios, hydraulic retention times and concentrations of mixed liquor total suspended solids. The bacterial community, dominated by Proteobacteria (20.9-53.8%) and Actinobacteria (20.6-57.6%), was very similar in both biomass fractions and able to maintain its functional stability under all the operating conditions, ensuring a successful and steady depuration process. Multivariate statistical analysis demonstrated that solids concentration, carrier filling ratio, temperature and organic matter concentration in the influent were the significant factors explaining population dynamics. Bacterial diversity increased as carrier filling ratio increased (from 20% to 35%, v/v), and solids concentration was the main factor triggering the shifts of the community structure. These findings provide new insights on the influence of operational parameters on the biology of the innovative MBMBRs.
Assuntos
Bactérias/metabolismo , Fenômenos Fisiológicos Bacterianos , Esgotos/microbiologia , Eliminação de Resíduos Líquidos , Bactérias/classificação , Bactérias/genética , Proteínas de Bactérias/genética , Reatores Biológicos , Membranas Artificiais , Análise Multivariada , FilogeniaRESUMO
In recent years, various technologies have been developed for the removal of nitrogen from wastewater that is rich in nitrogen but poor in organic carbon, such as the effluents from anaerobic digesters and from certain industries. These technologies have resulted in several patents. The core of these technologies is some of the processes and patents described in this paper: Aerobic denitrification, Sharon, Anammox, OLAND, CANON, NOx process, DEMON. More specifically, one of the first innovative options described for removing nitrogen include partial nitrification under aerobic conditions (partial Sharon process) followed by autotrophic anaerobic oxidation (Anammox process). The partial Sharon-Anammox process can be performed under alternating oxic and anoxic conditions in the same bioreactor or in two steps in two separate bioreactors. This overview focuses on the technical and biological aspects of these new types of treatment system, and compares them to other technologies. Given the fact that nitrification is a sensitive process, special attention is paid to conditions such as temperature, dissolved oxygen, hydraulic retention time, free ammonia, nitrous acid concentration, and pH. A discussion of the pros and cons of such treatment systems is also included since autotrophic nitrogen removal has advantages as well as drawbacks. The paper concludes with a discussion of future research that could improve these systems by enhancing performance and reducing costs.
Assuntos
Desnitrificação , Nitrificação , Nitrogênio/metabolismo , Esgotos/microbiologia , Eliminação de Resíduos Líquidos/métodos , Amônia/metabolismo , Processos Autotróficos , Reatores Biológicos , Fenômenos Microbiológicos , Oxirredução , Oxigênio/metabolismo , Patentes como AssuntoRESUMO
A cultivation independent approach (PCR-TGGE) was used to evaluate the occurrence of Archaea in four wastewater treatments based on technologies other than activated sludge, and to comparatively analyze their community structure. TGGE fingerprints (based on partial archaeal 16S-rRNA amplicons) were obtained from sludge samples taken from a pilot-scale aerated MBR fed with urban wastewater and operated under two different sets of conditions (MBR1 and MBR2 treatments), and also from biofilms sampled from two pilot-scale submerged biofilters (SBs) consisting of one aerated and one anoxic column each, fed with urban (USB treatment) or industrial (ISB treatment) wastewater, respectively. Analysis of TGGE fingerprints revealed clear and significant differences of the community structure of Archaea between the wastewater treatments studied, primarily according to wastewater origin and the type of technology. Thirty-two different band classes were detected among the 23 sludge and biofilm samples analyzed, from which five were selected as dominant or distinctive of the four treatments studied. Sixteen predominant TGGE bands were identified, revealing that all of them were related to methanogenic Archaea. Neither other Euryarchaeota groups nor Crenarchaeota members were identified amongst the 16S-rRNA fragments sequenced from separated TGGE bands.
Assuntos
Archaea/crescimento & desenvolvimento , Archaea/genética , Reatores Biológicos/microbiologia , Filtração/instrumentação , Membranas Artificiais , Eliminação de Resíduos Líquidos/instrumentação , Purificação da Água/instrumentação , Biodegradação Ambiental , Análise por Conglomerados , Eletroforese em Gel de Ágar , Genes Arqueais/genética , Filogenia , RNA Ribossômico 16S/genéticaRESUMO
Phosphatases, glucosidase, protease, esterase and dehydrogenase activities in a MBR (membrane bioreactor) system equipped with ultrafiltration membranes for the treatment of real urban wastewater were measured at different volatile suspended solid (VSS) concentrations, total suspended solid (TSS) concentrations, hydraulic retention times (HRT), temperatures and inflow rates. The results showed the capacity of the MBR system to remove COD and BOD(5) at TSS between 7200 and 13,300 mg/L; HRT values of 8.05 and 15.27 h; inflow rates of 14.67 and 27.81 L/h; and temperatures between 4 and 27 degrees C. The enzymatic activities are influenced by increases in VSS and TSS concentrations. These results suggest that the ability to get adapted to environmental changes of the bacterial populations and their microbial enzymatic activities is essential to understand the biological processes that occur in MBR systems and crucial for proper urban wastewater treatment when using MBR technologies.
Assuntos
Enzimas/metabolismo , Esgotos , Poluentes da Água/isolamento & purificação , Projetos PilotoRESUMO
A pilot scale submerged ultra-filtration membrane bioreactor (MBR) was used for the aerobic treatment of domestic wastewater over 9 months of year 2006 (28th March to 21st December). The MBR was installed at a municipal wastewater facility (EMASAGRA, Granada, Spain) and was fed with real wastewater. The experimental work was divided in 4 stages run under different sets of operation conditions. Operation parameters (total and volatile suspended solids, dissolved oxygen concentration) and environmental variables (temperature, pH, COD and BOD(5) of influent water) were daily monitored. In all the experiments conducted, the MBR generated an effluent of optimal quality complying with the requirements of the European Law (91/271/CEE 1991). A cultivation-independent approach (polymerase chain reaction-temperature gradient gel electrophoresis, PCR-TGGE) was used to analyze changes in the structure of the bacterial communities in the sludge. Cluster analysis of TGGE profiles demonstrated significant differences in community structure related to variations of the operation parameters and environmental factors. Canonical correspondence analysis (CCA) suggested that temperature, hydraulic retention time and concentration of volatile suspended solids were the factors mostly influencing community structure. 23 prominent TGGE bands were successfully reamplified and sequenced, allowing gaining insight into the identities of predominantly present bacterial populations in the sludge. Retrieved partial 16S-rRNA gene sequences were mostly related to the alpha-Proteobacteria, beta-Proteobacteria and gamma-Proteobacteria classes. The community established in the MBR in each of the four stages of operation significantly differed in species composition and the sludge generated displayed dissimilar rates of mineralization, but these differences did not influence the performance of the bioreactor (quality of the permeate). These data indicate that the flexibility of the bacterial community in the sludge and its ability to get adapted to environmental changes play an important role for the stable performance of MBRs.
Assuntos
Aerobiose , Reatores Biológicos , Membranas Artificiais , Microbiologia da Água , Projetos Piloto , Reação em Cadeia da Polimerase , Proteobactérias/classificação , Proteobactérias/genética , Proteobactérias/isolamento & purificação , RNA Ribossômico 16S/genética , UltrafiltraçãoRESUMO
A pilot-scale submerged membrane bioreactor was used for the treatment of domestic wastewater in order to study the influence of the variations in the concentration of volatile suspended solids (VSS) on the enzymatic activities (acid and alkaline phosphatases, glucosidase, protease, esterase, and dehydrogenase) and biodiversity of the bacterial community in the sludge. The influence of VSS concentration was evaluated in two separated experiments, which were carried out in two different seasons of the year (experiment 1 through spring-summer and experiment 2 through autumn-winter). Cluster analysis of the temperature gradient gel electrophoresis (TGGE) profiles demonstrated that the community composition was significantly different in both experiments. Within the same experiment, the bacterial community experienced sequential shifts as the biomass accumulated, as shown by the evolution of the population profiles through time as VSS concentration increased. All enzymatic activities studied were significantly lower during experiment 2, except for glucosidase. Concentrations of VSS over 8 g/l induced a strong descent of all enzymatic activities, which overlapped with a significant modification of the community composition. Sequences of the major TGGE bands were identified as representatives of the Alpha-proteobacteria, filamentous bacteria (Thiotrix), and nitrite oxidizers (Nitrospira). Some sequences which were poorly related to any validated bacterial taxon were obtained.