1.
Nat Methods
; 7(6): 451-3, 2010 Jun.
Artigo
em Inglês
| MEDLINE
| ID: mdl-20418868
RESUMO
We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.