Weekend sleep after early and later school start times confirmed a model-predicted failure to catch up sleep missed on weekdays.

BACKGROUND: Many people believe they sleep for longer time on weekend nights to make up for sleep lost on weekdays. However, results of simulations of risetimes and bedtimes on weekdays and weekends with a sleep-wake regulating model revealed their inability to prolong weekend sleep. In particular, they predicted identical durations of weekend sleep after weeks with relatively earlier and relatively later risetime on weekdays. In the present study, this paradoxical prediction was empirically confirmed. METHODS: Times in bed were calculated from weekday and weekend risetimes and bedtimes in pairs of samples of students with early and later school start time and in subsets of samples from 7 age groups with weekday risetime earlier and later than 7:00 a.m. RESULTS: Among 35 pairs of students, mean age ± standard deviation was 14.5 ± 2.9 years and among the age group samples, 21.6 ± 14.6 years. As predicted by the simulations, times in bed on weekends were practically identical in the samples with early and later school start time and in two subsets with earlier and later weekday risetime. CONCLUSIONS: The model-based simulations of sleep times can inform an individual about an amount of irrecoverable loss of sleep caused by an advance shift of wakeups on weekdays.

Sleep during "lockdown" highlighted the need to rethink the concept of weekend catch-up sleep.

PURPOSE: Many people believe in their ability to sleep for longer time on weekends to make up for sleep lost due to early wakeups on weekdays. This widely held belief was not supported by the simulations of rise- and bedtimes on weekdays and weekends with a sleep-wake regulating model. The simulations suggested the inability to extend sleep on any of two weekend nights and they predicted identical weekend sleep durations for weeks with relatively earlier and relatively later weekday risetimes. By April 2020, about half of the world's population was under some form of "lockdown" due to the COVID-19 pandemic. This "lockdown" provided a new opportunity to demonstrate the predictive power of the sleep-wake regulating models. Therefore, the purpose of this report was to support the prediction of identity of weekend sleep durations after weeks with earlier and later weekday wakeups. METHODS: Weekend and weekday rise- and bedtimes before and during "lockdown" for 31 samples were taken from recent journal publications. Time in bed on weekends and 12 other measures of sleep duration and timing were calculated and simulated. RESULTS: For only one of 13 measures, weekend time in bed, statistical analysis did not yield a statistically significant difference between the estimates obtained before and during "lockdown". The model-based simulations pointed to the 0.3-h delay of the sleep-wake cycle in response to the 1-h delay of weekday risetime during "lockdown". CONCLUSION: The model-based prediction was confirmed, thus, highlighting again the necessity to rethink the concept of weekend catch-up sleep.

Prospects of Testing Diurnal Profiles of Expressions of TSH-R and Circadian Clock Genes in Thyrocytes for Identification of Preoperative Biomarkers for Thyroid Carcinoma.

Thyroid Nodules (TN) are frequent but mostly benign, and postoperative rate of benign TN attains the values from 70% to 90%. Therefore, there is an urgent need for identification of reliable preoperative diagnosis markers for patients with indeterminate thyroid cytology. In this study, an earlier unexplored design of research on preoperative biomarkers for thyroid malignancies was proposed. Evaluation of reported results of studies addressing the links of thyroid cancer to the circadian clockwork dysfunctions and abnormal activities of Thyroid-Stimulating Hormone (TSH) and its receptor (TSH-R) suggested diagnostic significance of such links. However, there is still a gap in studies of interrelationships between diurnal profiles of expression of circadian clock genes and TSH-R in indeterminate thyroid tissue exposed to different concentrations of TSH. These interrelationships might be investigated in future in vitro experiments on benign and malignant thyrocytes cultivated under normal and challenged TSH levels. Their design requires simultaneous measurement of diurnal profiles of expression of both circadian clock genes and TSH-R. Experimental results might help to bridge previous studies of preoperative biomarkers for thyroid carcinoma exploring diagnostic value of diurnal profiles of serum TSH levels, expression of TSH-R, and expression of circadian clock genes.

Linking stages of non-rapid eye movement sleep to the spectral EEG markers of the drives for sleep and wake.

The conventional staging classification reduces all patterns of sleep polysomnogram signals to a small number of yes-or-no variables labeled wake or a stage of sleep (e.g., W, N1, N2, N3, and R for wake, the first, second, and third stages of non-rapid eye movement sleep and rapid eye movement sleep, respectively). However, the neurobiological underpinnings of such stages remained to be elucidated. We tried to evaluate their link to scores on the first and second principal components of the EEG spectrum (1PCS and 2PCS), the markers of two major groups of promoters/inhibitors of sleep/wakefulness delineated as the drives for sleep and wake, respectively. On two occasions, polysomnographic records were obtained from 69 university students during 50-min afternoon naps and 30-s stage epochs were assigned to 1PCS and 2PCS. Results suggested two dimensionality of the structure of individual differences in amounts of stages. Amount of N1 loaded exclusively on one of two dimensions associated with 1PCS, amounts of W and N2 loaded exclusively on another dimension associated with 2PCS, and amount of N3 was equally loaded on both dimensions. Scores demonstrated stability within each stage, but a drastic change in just one of two scores occurred during transitions from one stage to another on the way from wakefulness to deeper sleep (e.g., 2PCS changed from >0 to <0 during transition W→N1, 1PCS changed from <0 to >0 during transition N1→N2). Therefore, the transitions between stages observed during short naps might be linked to rapid changes in the reciprocal interactions between the promoters/inhibitors of sleep/wakefulness.NEW & NOTEWORTHY In the present nap study, two dimensionality of the structure of individual differences in sleep stages was revealed. These results also suggested that individual variation in the sleep and wake drives associated with the first and second principal components of the EEG spectrum might underlie this structure. It seemed that each stage might be related to a certain, stage-specific combination of wake-sleep promoting/inhibiting influences associated with these drives for sleep and wake.

Differential relationship of two measures of sleepiness with the drives for sleep and wake.

PURPOSE: Since disagreement has been found between an objective sleep propensity measured by sleep onset latency (SOL) and subjective sleepiness assessment measured by the Epworth sleepiness scale (ESS) score, distinct underlying causes and consequences were suggested for these two sleepiness measures. We addressed the issue of validation of the ESS against objective sleepiness and sleep indexes by examining the hypothesis that these two sleepiness measures are disconnected due to their differential relationship with the antagonistic drives for sleep and wake. METHODS: The polysomnographic records of 50-min napping attempts were collected from 27 university students on three occasions. Scores on the first and second principal components of the electroencephalographic (EEG) spectrum were calculated to measure the sleep and wake drives, respectively. Self-assessments of subjective sleepiness and sleep were additionally collected in online survey of 633 students at the same university. RESULTS: An ESS score was disconnected with the polysomnographic and self-assessed SOL in the nap study and online survey, respectively. An ESS score but not SOL was significantly linked to the spectral EEG measure of the sleep drive, while SOL but not ESS showed a significant association with the spectral EEG measure of the opposing wake drive. CONCLUSIONS: Each of two sleepiness measures was validated against objective indicators of the opposing sleep-wake regulating processes, but different underlying causes were identified for two distinct aspects of sleepiness. A stronger sleep drive and a weaker opposing drive for wake seem to contribute to a higher ESS score and to a shorter SOL, respectively.

Differences between male and female university students in sleepiness, weekday sleep loss, and weekend sleep duration.

INTRODUCTION: Women and men experience sleep differently and the difference in intrinsic desire for sleep might underlie some of the observed male-female differences. The objective of this cross-sectional questionnaire study of university students was to determine male-female differences in self-reported sleepiness and sleep-wake patterns. METHODS: Five questionnaires were completed by 1650 students at four Russian universities. RESULTS: Compared to male students, female students reported a lower subjective sleep quality score, had a higher morning sleepability score and lower nighttime and daytime wakeability scores. They more often reported excessive daytime sleepiness and expected to be sleepier at any time of the day with the largest male-female difference around the times of sleep onset and offset. On free days, they reported a longer sleep duration and an earlier sleep onset. Free-weekday difference was larger for sleep duration and smaller for sleep onset. Such male-female differences showed similarity to the differences observed in university and high school students from different countries around the globe. There was no significant male-female difference in weekly averaged sleep duration, weekday sleep duration, hours slept, midpoint of sleep on free days, free-weekday difference in sleep offset, social jetlag, and morningness-eveningness score. Therefore, when studies rely on these self-reports, the most salient male-female differences might not be immediately evident. CONCLUSIONS: It seems that the intrinsic desire for longer sleep duration might contribute to a higher susceptibility of female students to weekday sleep loss. Among these students, negative effects of reduced sleep duration might be more common and more detrimental.

State- and trait-like variation in morning and evening components of morningness-eveningness in winter depression.

PURPOSE: Evening preference (eveningness) can be a risk factor for depression and a shift toward morning preference (morningness) can occur in response to treatment. A study of winter depression provides possibility to longitudinally evaluate state- and trait-like variation in morningness-eveningness during treatment in winter and remission in summer. MATERIAL AND METHODS: Female patients with winter depression and controls without a psychiatric history (n = 54 and 32 with mean age ± standard deviation of 34.4 ± 11.0 and 35.7 ± 9.5 years, respectively) were treated with two-hour bright light for a week during winter period. Some of them (n = 40 and 19, respectively) were then restudied in summer. Measures obtained during the winter period from patients before and after treatment were compared to those obtained from controls and from the same patients in the summer period. Among compared measures, there were self-assessments of state- and trait-like differences in morning and evening components of morningness-eveningness. RESULTS: The groups of depressed patients and controls differed in self-assessments of morning but not evening component of morningness-eveningness. The difference in state-like variation in morning component became non-significant after treatment and in summer. On the other hand, trait-like variation in this component demonstrated adequate test-retest (winter-summer) reliability, i.e. a shift toward trait-like eveningness persisted in patients in the summer. CONCLUSIONS: The observed normalization of state-like variation in morning component of morning-evening preference can be mainly explained by the disappearance of such depressive symptoms as lack of energy, social withdrawal, loss of interest in once enjoyable activities, etc.

Alpha attenuation soon after closing the eyes as an objective indicator of sleepiness.

Attenuation of alpha rhythm in occipital derivation serves as a reliable electroencephalographic (EEG) marker of sleep onset. If such attenuation not only coincides with but also anticipates sleep onset, objective evaluation of sleepiness of permanently waking individuals might be facilitated by probing alpha attenuation immediately after closing eyes. We tested whether alpha-based EEG indexes reflect self-scored sleepiness and objectively measured waking ability. A total of 15 young adults self-scored their sleepiness before and after recording of their resting EEG with a 2-h interval in the course of 43-61-h wakefulness. For each EEG record, power spectra were calculated on 2-min intervals of the eyes open section and on five following 1-min intervals of the eyes closed section. Aking ability was assessed as latency to sleep onset marked by zero-crossing decline of such EEG indexes as alpha-theta power difference in occipital derivation and scores on the second principal component of the EEG spectrum in frontal and occipital derivations. Alpha attenuation during the first minute with eyes closed was found to be significantly related to the levels of subjective sleepiness and waking ability. The relationship between alpha attenuation and subjective sleepiness was confirmed by analysing 1-min eyes closed EEG recordings obtained with a 3-h interval in the course of 24-h sustained wakefulness of 130 adolescents and adults. We concluded that such 1-min eyes closed EEG recordings might be used for simple and quick measurements of sleepiness and waking ability in experimental and field studies of permanently waking individuals.

Can the Brain's Thermostatic Mechanism Generate Sleep-Wake and NREM-REM Sleep Cycles? A Nested Doll Model of Sleep-Regulating Processes.

Evidence is gradually accumulating in support of the hypothesis that a process of thermostatic brain cooling and warming underlies sleep cycles, i.e., the alternations between non-rapid-eye-movement and rapid-eye-movement sleep throughout the sleep phase of the sleep-wake cycle. A mathematical thermostat model predicts an exponential shape of fluctuations in temperature above and below the desired temperature setpoint. If the thermostatic process underlies sleep cycles, can this model explain the mechanisms governing the sleep cyclicities in humans? The proposed nested doll model incorporates Process s generating sleep cycles into Process S generating sleep-wake cycles of the two-process model of sleep-wake regulation. Process s produces ultradian fluctuations around the setpoint, while Process S turns this setpoint up and down in accord with the durations of the preceding wake phase and the following sleep phase of the sleep-wake cycle, respectively. Predictions of the model were obtained in an in silico study and confirmed by simulations of oscillations of spectral electroencephalographic indexes of sleep regulation obtained from night sleep and multiple napping attempts. Only simple-inverse exponential and exponential-functions from the thermostatic model were used for predictions and simulations of rather complex and varying shapes of sleep cycles during an all-night sleep episode. To further test the proposed model, experiments on mammal species with monophasic sleep are required. If supported, this model can provide a valuable framework for understanding the involvement of sleep-wake regulatory processes in the mechanism of thermostatic brain cooling/warming.

Two Old Wild-Type Strains of Drosophila melanogaster Can Serve as an Animal Model of Faster and Slower Aging Processes.

BACKGROUND: Drosophila melanogaster provides a powerful platform to study the physiology and genetics of aging, i.e., the mechanisms underpinnings healthy aging, age-associated disorders, and acceleration of the aging process under adverse environmental conditions. Here, we tested the responses of daily rhythms to age-accelerated factors in two wild-type laboratory-adapted strains, Canton-S and Harwich. METHODS: On the example of the 24 h patterns of locomotor activity and sleep, we documented the responses of these two strains to such factors as aging, high temperature, carbohydrate diet, and diet with different doses of caffeine-benzoate sodium. RESULTS: The strains demonstrated differential responses to these factors. Moreover, compared to Canton-S, Harwich showed a reduced locomotor activity, larger amount of sleep, faster rate of development, smaller body weight, lower concentrations of main sugars, lower fecundity, and shorter lifespan. CONCLUSIONS: It might be recommended to use at least two strains, one with a relatively fast and another with a relatively slow aging process, for the experimental elaboration of relationships between genes, environment, behavior, physiology, and health.

Aging, Sleep and Sleepiness Self-Assessment, and the Underlying Drives for Sleep and Wake.

In 2016, a mini-issue of Current Aging Science (CAS) entitled "Effects of Aging on Circadian and Sleep Timing" has been published to report the state of the art in the studies of the effects of aging on the circadian and sleep regulating processes. The emphasis has been given to the regulatory processes involved in age-specific problems with sleep timing, continuity, and duration. Such problems can serve as targets for novel treatments for geriatric and sleep disorders. In the following 6 years, some new findings provided further insight into the previously recognized age-specific problems and highlighted new questions of research on the relation of aging to the regulatory mechanisms underlying circadian rhythmicity, sleep, and sleepiness. The theoretic framework of one of the directions of this research regards the interaction between the competing drives for sleep and wake as one of the basic features of regulatory processes underlying circadian rhythms, including such rhythms as the sleep-wake cycle and the diurnal variation in alertnesssleepiness levels. Here, earlier and more recently highlighted questions of the research in this framework were briefly reviewed.

Reaction of the endogenous regulatory mechanisms to early weekday wakeups: a review of its popular explanations in light of model-based simulations.

Introduction: Several widely held explanations of the mechanisms underlying the responses of endogenous sleep-wake-regulating processes to early weekday wakeups have been proposed. Here, they were briefly reviewed and validated against simulations based on the rhythmostatic version of a two-process model of sleep-wake regulation. Methods: Simulated sleep times on weekdays and weekends were compared with the times averaged over 1,048 samples with either earlier or later weekday risetimes. In total, 74 paired samples were collected before and during lockdown, and 93 paired samples were collected during early and later school start times. Results: The counterintuitive predictions of the simulations included the following: 1) only one night of ad lib sleep is sufficient to restore the endogenously determined sleep times after 1 day/5 days of larger/smaller reduction/extension of the sleep/wake phase of the circadian sleep-wake cycle; 2) sleep loss on weekdays is irrecoverable; 3) irrespective of the amount of such deadweight loss, sleep on weekends is not prolonged; and 4) the control of the circadian clocks over the sleep-wake cyclicity is not disrupted throughout the week. Discussion: The following popular explanations of the gaps between weekends and weekdays in sleep timing and duration were not supported by these simulations: 1) early weekday wakeups cause "social jetlag," viewed as the weekend and weekday (back and forth) shifts of the sleep phase relative to the unchanged phase of the circadian clocks, and 2) early weekday wakeups cause an accumulation of "sleep debt paid back" on weekends, or, in other terms, people can "catch-up" or "compensate" sleep on weekends.

A Review of Evidence for the Involvement of the Circadian Clock Genes into Malignant Transformation of Thyroid Tissue.

(1) Background: In 2013, the results of a pioneer study on abnormalities in the levels and circadian rhythmicity of expression of circadian clock genes in cancerous thyroid nodules was published. In the following years, new findings suggesting the involvement of circadian clockwork dysfunction into malignant transformation of thyroid tissue were gradually accumulating. This systematic review provides an update on existing evidence regarding the association of these genes with thyroid tumorigenesis. (2) Methods: Two bibliographic databases (Scopus and PubMed) were searched for articles from inception to 20 March 2023. The reference lists of previously published (nonsystematic) reviews were also hand-searched for additional relevant studies. (3) Results: Nine studies published between 2013 and 2022 were selected. In total, 9 of 12 tested genes were found to be either up- or downregulated. The list of such genes includes all families of core circadian clock genes that are the key components of three transcriptional-translational feedback loops of the circadian clock mechanism (BMAL1, CLOCK, NPAS2, RORα, REV-ERBα, PERs, CRYs, and DECs). (4) Conclusions: Examination of abnormalities in the levels and circadian rhythmicity of expression of circadian clock genes in thyroid tissue can help to reduce the rate of inadequate differential preoperative diagnosis for thyroid carcinoma.

Motus Vita Est: Fruit Flies Need to Be More Active and Sleep Less to Adapt to Either a Longer or Harder Life.

BACKGROUND: Activity plays a very important role in keeping bodies strong and healthy, slowing senescence, and decreasing morbidity and mortality. Drosophila models of evolution under various selective pressures can be used to examine whether increased activity and decreased sleep duration are associated with the adaptation of this nonhuman species to longer or harder lives. METHODS: For several years, descendants of wild flies were reared in a laboratory without and with selection pressure. To maintain the "salt" and "starch" strains, flies from the wild population (called "control") were reared on two adverse food substrates. The "long-lived" strain was maintained through artificial selection for late reproduction. The 24 h patterns of locomotor activity and sleep in flies from the selected and unselected strains (902 flies in total) were studied in constant darkness for at least, 5 days. RESULTS: Compared to the control flies, flies from the selected strains demonstrated enhanced locomotor activity and reduced sleep duration. The most profound increase in locomotor activity was observed in flies from the starch (short-lived) strain. Additionally, the selection changed the 24 h patterns of locomotor activity and sleep. For instance, the morning and evening peaks of locomotor activity were advanced and delayed, respectively, in flies from the long-lived strain. CONCLUSION: Flies become more active and sleep less in response to various selection pressures. These beneficial changes in trait values might be relevant to trade-offs among fitness-related traits, such as body weight, fecundity, and longevity.

Evening chronotype, insufficient weekday sleep, and weekday-weekend gap in sleep times: What is really to blame for a reduction in self-perceived health among university students?

The association of insufficient sleep with reduced self-perceived health was previously well established. Moreover, it was sometimes shown that the indicators of poorer health were significantly related to chronotype and weekday-weekend gaps in sleep timing and duration. It remains to be elucidated, however, whether chronotype and these gaps can contribute to the reduced health self-ratings independently from shortened sleep duration or, alternatively, their relationship with health can be simply explained by their association with insufficient sleep on weekdays. In an online survey, we tested whether the self-rated health of university students can be predicted by several individual characteristics of the sleep-wake cycles, such as chronotype, weekday and weekend sleep times, weekday-weekend gap in sleep times, sleepability and wakeability at different times of the day, etc. Responses to a question about general health and to items of several questionnaires for chronobiological assessment were collected from smartphones of 1582 university students (mean age ± standard deviation was 19.5 ± 1.7 y). The results of regression analyses suggested that lower odds of having good self-rated health were significantly associated with an earlier weekday risetime, a later weekday bedtime, and, consequently, a shorter weekday time in bed. After accounting for weekday sleep, self-rated health showed significant association with neither chronotype nor weekday-weekend differences in sleep duration and timing. Besides, the adverse health effects of reduced weekday sleep were independent from the significant adverse effects of several other individual sleep-wake characteristics including poorer nighttime sleepability and lower daytime wakeability. We concluded that university students perceive the negative health impacts of losing sleep by waking up early on weekdays irrespective of their night sleep quality and daytime level of alertness. Their chronotype and weekday-weekend difference in sleep times might not be among significant contributors to this perception. It is of practical importance to consider the reduction of weekday sleep losses among the interventions aimed at preventing sleep and health problems.

Prevalence of Insomnia and Sleep Habits during the First and Second Wave of COVID-19 in Belgium.

Belgium has one of the highest numbers of COVID-19 cases per 1 million inhabitants. The pandemic has led to significant societal changes with repercussions on sleep and on mental health. We aimed to investigate the effect of the first and the second wave of COVID-19 on the sleep of the Belgian populationWe launched two online questionnaires, one during the first lockdown (7240 respondents) and one during the second (3240 respondents), to test differences in self-reported clinical insomnia (as measured by the Insomnia Severity Index) and sleep habits during the two lockdowns in comparison with the pre-COVID period. The number of persons with clinical insomnia rose during the first lockdown (19.22%) and further during the second (28.91%) in comparison with pre-lockdown (7.04-7.66%). Bed and rise times were delayed and there was an increased time in bed and sleep onset latency. There was further a decrease in total sleep time and in sleep efficiency during both confinements. The prevalence of clinical insomnia quadrupled during the second wave in comparison with the pre-lockdown situation. Sleep habits were most altered in the younger population, indicating a greater risk for this group to develop a sleep-wake rhythm disorder.

What Can Make the Difference Between Chronotypes in Sleep Duration? Testing the Similarity of Their Homeostatic Processes.

The two-process conceptualization of sleep-wake regulation suggests that the biological underpinnings of the differences between morning and evening types in sleep timing and duration might be related to either the circadian process or the homeostatic process or both. The purpose of this report was to test whether morning and evening types might have similar homeostatic processes to achieve such ultimate goal of homeostatic sleep regulation as taking an adequate amount of sleep on free days. Weekend and weekday rise- and bedtimes reported for 50 paired samples of morning and evening types were averaged and simulated with a model of sleep-wake regulation. In morning and evening types of the same age, the homeostatic components of the sleep-wake regulation were found to be identical. Therefore, the difference in the circadian process between chronotypes of similar age can account for the observed differences between them in sleep timing and duration on weekdays and weekends. It was also demonstrated that the model-based simulations might have practical implications for informing an individual about the extent of unrecoverable reduction of his/her sleep on weekdays.

When early and late risers were left to their own devices: six distinct chronotypes under "lockdown" remained dissimilar on their sleep and health problems.

Under national "lockdown," the habitual late risers need not wake up early, and, similarly to the early risers, they don't lose much sleep on weekdays. We tested whether, despite a decrease in weekday sleep loss, the difference between distinct chronotypes in health and sleep problems persisted during "lockdown." Two online surveys were conducted from 10th to 20th of May, 2020 and 2021, one of them after 6 non-working weeks and another after 14 working weeks (during and after "lockdown," respectively). Participants were students of the same grade at the same university department (572 and 773, respectively). The self-assessments included the Single-Item Chronotyping (SIC) designed for self-choosing chronotype among several their short descriptions and several questions about general health, mood state, outdoors and physical activity, and sleep concerns. The results suggested that the responses to each of the questions were not randomly distributed over 6 distinct chronotypes. Such a nonrandomness was identified within each of three pairs of these chronotypes, evening vs. morning types (with a rising throughout the day vs. a falling level of alertness, respectively), afternoon vs. napping types (with a peak vs. a dip of alertness in the afternoon, respectively), and vigilant vs. lethargic types (with the levels of alertness being permanently high vs. low, respectively). Morning, afternoon, and vigilant types reported healthier sleep/mood/behavior/habits than three other types. The most and the least healthy sleep/mood/behavior/habits were reported by morning and evening types, respectively. These relationships with health and sleep problems and the frequencies of 6 chronotypes remained unchanged after "lockdown." Such results, in particular, suggested that the association of evening types with poorer health and sleep might not be attributed to a big amount of weekday sleep loss. The accounting for this association might help in designing interventions purposed on reduction of sleep and health problems.

Association between the Effects of High Temperature on Fertility and Sleep in Female Intra-Specific Hybrids of Drosophila melanogaster.

Humans and fruit flies demonstrate similarity in sleep-wake behavior, e.g., in the pattern of sleep disturbances caused by an exposure to high temperature. Although research has provided evidence for a clear connection between sleeping problems and infertility in women, very little is known regarding the mechanisms underlying this connection. Studies of dysgenic crosses of fruit flies revealed that an exposure to elevated temperature induces sterility in female intra-specific hybrids exclusively in one of two cross directions (progeny of Canton-S females crossed with Harwich males). Given the complexity and limitations of human studies, this fruit flies' model of temperature-sensitive sterility might be used for testing whether the effects of high temperature on fertility and on 24-h sleep pattern are inter-related. To document this pattern, 315 hybrids were kept for at least five days in constant darkness at 20 °C and 29 °C. No evidence was found for a causal link between sterility and sleep disturbance. However, a diminished thermal responsiveness of sleep was shown by females with temperature-induced sterility, while significant responses to high temperature were still observed in fertile females obtained by crossing in the opposite direction (i.e., Canton-S males with Harwich females) and in fertile males from either cross.

Chronobiological traits predict the restrained, uncontrolled, and emotional eating behaviors of female university students.

Although significant associations between diurnal preference and restrained eating behaviors were previously reported, such reports are scarce and, in some respects, inconclusive. In this cross-sectional survey of 567 female university students aged between 17 and 23 years, we tried to clarify and extend the previous findings on chronobiological correlates of these behaviors. We administered the three-Factor-Eating-Questionnaire Revised and three questionnaires designed to assess trait-, ability-, and state-like differences in the domain of chronobiology, the Morningness-Eveningness Questionnaire (MEQ), the Sleep-Wake Pattern Assessment Questionnaire, and the Munich ChronoType Questionnaire, respectively. Statistical analyses included factor, correlation, and regression analyses. We found that any of three aspects of unhealthy eating behaviors (i.e., lack of cognitive eating restraint, uncontrolled eating, and emotional eating) was linked to one or more dimensions of individual chronobiological differences. We explained the previously reported inconclusive results by the differential relationship of two subconstructs of diurnal preferences to eating behaviors. For instance, such relationship was found for two (morning and evening) subscales of the MEQ. Cognitive eating restraint and uncontrolled eating were related to the morning subscale, while emotional eating was related to the evening subscale. These associations were supported by the associations revealed for morning vs. evening components of earliness-lateness assessed with two other questionnaires, (e.g., morning lateness and sleep offset vs. evening lateness and sleep onset, respectively). We conclude that unhealthy eating behaviors seem to be linked to unhealthy sleep-wake habits and behaviors and to inabilities to wake or sleep on demand at certain times of the day.