RESUMO
Heme-oxygenase 1 (HO-1) is a stress-response protein with anti-inflammatory activity. This study has examined the regulation of HO-1 expression by the anti-inflammatory factor, interleukin (IL)-10 and whether HO-1 could account for the function of the cytokine. IL-10-induced expression of HO-1 required the activation of signal transducer and activator of transcription (STAT)-3 but not p38 mitogen-activated protein kinase. However, expression of HO-1 also required the activation of the phosphatidylinositol-3 kinase pathway, a signaling mechanism not required for the anti-inflammatory activity of IL-10. Moreover, induction of HO-1 expression was not restricted to IL-10, as IL-6, a cytokine known to activate STAT-3, could also induce the protein. In human macrophages, lipopolysaccharide inhibited HO-1 expression induced by IL-10. Also, inhibition of HO-1 activity by the specific inhibitor zinc-II-protoporphyrin-IX had no effect on the anti-inflammatory function of IL-10. In summary, although IL-10 does regulate HO-1 expression, it does not appear to play a significant role in the anti-inflammatory activity of the cytokine.
Assuntos
Anti-Inflamatórios/metabolismo , Proteínas de Ligação a DNA/metabolismo , Heme Oxigenase (Desciclizante)/metabolismo , Interleucina-10/metabolismo , Macrófagos/enzimologia , Fosfatidilinositol 3-Quinases/metabolismo , Transativadores/metabolismo , Animais , Anti-Inflamatórios/imunologia , Células Cultivadas , Proteínas de Ligação a DNA/efeitos dos fármacos , Retroalimentação Fisiológica/efeitos dos fármacos , Retroalimentação Fisiológica/imunologia , Heme Oxigenase (Desciclizante)/efeitos dos fármacos , Heme Oxigenase (Desciclizante)/imunologia , Heme Oxigenase-1 , Humanos , Inflamação/induzido quimicamente , Inflamação/enzimologia , Inflamação/imunologia , Interleucina-10/imunologia , Interleucina-10/farmacologia , Interleucina-6/metabolismo , Interleucina-6/farmacologia , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Masculino , Proteínas de Membrana , Camundongos , Camundongos Endogâmicos BALB C , Monócitos/efeitos dos fármacos , Monócitos/enzimologia , Monócitos/imunologia , Fosfatidilinositol 3-Quinases/efeitos dos fármacos , Protoporfirinas/farmacologia , Fator de Transcrição STAT3 , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/imunologia , Transativadores/efeitos dos fármacosRESUMO
Teratomas are neoplasms of the embryonic tissues that typically arise in the gonadal and sacrococcygeal regions of adults and children. Primary adult retroperitoneal teratomas are rare and demand challenging management options. We report a case of a unilateral primary retroperitoneal mature cystic teratoma mimicking an adrenal mass in a 28-year-old female patient. Complete resection of the mass was performed by a laparotomy approach. Because of the risk of malignancy, follow-up radiographic studies were performed to ensure the oncologic efficacy of resection. The patient remains free of recurrence to date.