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1.
Clin Exp Ophthalmol ; 45(5): 520-528, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28013525

RESUMO

BACKGROUND: Microinvasive glaucoma surgery (MIGS) is a relatively new addition to the glaucoma treatment paradigm. Small metallic stents are inserted into the trabecular meshwork in order to increase aqueous humour drainage. MIGS procedures are rapidly being adopted owing to a more favourable side effect profile when compared with traditional surgery. Remarkably, this rapid rate of utilization has occurred without any published studies on the effect of metal alloys used in these stents on human trabecular meshwork cells (HTMCs). Therefore, this study aimed to determine the effect of candidate metal alloys for MIGS on HTMC morphology, viability and function. METHODS: Human trabecular meshwork cells were cultured on the surfaces of titanium (polished and sandblasted), a titanium-nickel (nitinol) alloy and glass (as control substratum). Fluorescence imaging was used to assess cell morphology and spreading. A lactate dehydrogenase cytotoxicity assay, cell death detection ELISA, MTT cell viability assay, BrdU cell proliferation assay and fibronectin ELISA were also conducted. RESULTS: Cells cultured on sandblasted titanium exhibited significantly greater spreading than cells cultured on other substrata. In comparison, HTMCs cultured on nitinol displayed poor spreading. Significantly more cell death, by both necrosis and apoptosis, occurred on nitinol than on titanium and glass. Also, cell viability and proliferation were suppressed on nitinol compared with titanium or glass. Finally, HTMCs on both titanium and nitinol produced greater amounts of fibronectin than cells grown on glass. CONCLUSIONS: Substratum topography and metal alloy composition were found to impact morphology, viability and function of primary HTMC cultures.


Assuntos
Ligas/farmacologia , Cirurgia Filtrante/métodos , Implantes para Drenagem de Glaucoma , Glaucoma/patologia , Procedimentos Cirúrgicos Minimamente Invasivos , Malha Trabecular/ultraestrutura , Apoptose , Proliferação de Células , Sobrevivência Celular , Células Cultivadas , Colorimetria , Ensaio de Imunoadsorção Enzimática , Glaucoma/metabolismo , Glaucoma/cirurgia , Humanos , Microscopia Eletrônica de Varredura , Microscopia de Fluorescência , Malha Trabecular/efeitos dos fármacos , Malha Trabecular/metabolismo
2.
Implant Dent ; 26(2): 225-231, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27918313

RESUMO

PURPOSE: Mechanical loosening remains a common complication associated with mini-implant failure. The purpose of this study was to compare common mechanical measures of mini-implant stability to determine their association and reliability. MATERIALS AND METHODS: Ninety self-drilling orthodontic mini-implants from 6 manufacturers were inserted into artificial bone blocks. Insertion torques (ITs) and Periotest values (PVs) were measured. Subsequently, mini-implants underwent pull-out testing for measures of pull-out load (POL) and screw displacement (ScrD). Stability measurements were compared using one-way ANOVA, associations among them were assessed using correlation analyses, and reliability was evaluated using coefficients of variation (COVs). RESULTS: Variations in stability of mini-implants were found, specific to the mechanical measure used for assessment (P < 0.05). The strongest correlations were found between IT and PV (r = -0.68) and between IT and POL (r = 0.66). Overall, PV showed the greatest variability (COV: 11%-100%) compared with IT (≤11%), POL (≤4%), and ScrD (≤19%). CONCLUSIONS: IT, PV, and POLs only agreed moderately in their assessment of mini-implant stability, and Periotest showed the least reliability in predicting mini-implant stability. As such, independent and interchangeable use of these stability measures should be avoided.


Assuntos
Implantes Dentários , Análise do Estresse Dentário/métodos , Falha de Restauração Dentária , Humanos , Procedimentos de Ancoragem Ortodôntica/métodos
3.
Molecules ; 22(11)2017 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-29143782

RESUMO

In this study bio-based bio-phenol-formaldehyde (BPF) resoles were prepared using hydrolytically depolymerized Kraft lignin (DKL) as bio-phenol to partially substitute phenol. The effects of phenol substitution ratio, weight-average molecular weight (Mw) of DKL and formaldehyde-to-phenol (F/P) ratio were also investigated to find the optimum curing temperature for BPF resoles. The results indicated that DKL with Mw ~ 1200 g/mol provides a curing temperature of less than 180 °C for any substitution level, provided that F/P ratios are controlled. Incorporation of lignin reduced the curing temperature of the resin, however, higher Mw DKL negatively affected the curing process. For any level of lignin Mw, the curing temperature was found to increase with lower F/P ratios at lower phenol substitution levels. At 25% and 50% phenol substitution, increasing the F/P ratio allows for synthesis of resoles with lower curing temperatures. Increasing the phenol substitution from 50% to 75% allows for a broader range of lignin Mw to attain low curing temperatures.


Assuntos
Formaldeído/química , Lignina/química , Fenol/química , Hidrólise , Temperatura
4.
J Prosthet Dent ; 116(2): 277-85, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26996934

RESUMO

STATEMENT OF PROBLEM: Most ceramic abutments are fabricated from yttria-stabilized tetragonal zirconia (Y-TZP). However, Y-TZP undergoes hydrothermal degradation, a process that is not well understood. PURPOSE: The purpose of this in vitro study was to assess the effects of artificial aging conditions on the fracture load, phase stability, and surface microstructure of a Y-TZP abutment. MATERIAL AND METHODS: Thirty-two prefabricated Y-TZP abutments were screwed and tightened down to external hexagon implants and divided into 4 groups (n = 8): C, control; MC, mechanical cycling (1×10(6) cycles; 10 Hz); AUT, autoclaving (134°C; 5 hours; 0.2 MPa); and TC, thermal cycling (10(4) cycles; 5°/55°C). A single-load-to-fracture test was performed at a crosshead speed of 0.5 mm/min to assess the assembly's resistance to fracture (ISO Norm 14801). X-ray diffraction (XRD) analysis was applied to observe and quantify the tetragonal-monoclinic (t-m) phase transformation. Representative abutments were examined with high-resolution scanning electron microscopy (SEM) to observe the surface characteristics of the abutments. Load-to-fracture test results (N) were compared by ANOVA and Tukey test (α=.05). RESULTS: XRD measurements revealed the monoclinic phase in some abutments after each aging condition. All the aging conditions reduced the fracture load significantly (P<.001). Mechanical cycling reduced the fracture load more than autoclaving (P=.034). No differences were found in the process of surface degradation among the groups; however, the SEM detected grinding-induced surface flaws and microcracks. CONCLUSIONS: The resistance to fracture and the phase stability of Y-TZP implant abutments were susceptible to hydrothermal and mechanical conditions. The surface microstructure of Y-TZP abutments did not change after aging conditions.


Assuntos
Ítrio/química , Zircônio/química , Dente Suporte , Análise do Estresse Dentário , Humanos , Técnicas In Vitro , Microscopia Eletrônica de Varredura , Propriedades de Superfície
5.
J Can Dent Assoc ; 80: e12, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24598328

RESUMO

OBJECTIVE: This study was undertaken to determine the effect of temperature on the microhardness and viscosity of 4 resin composite materials. METHODS: To investigate microhardness, samples of each of the 4 composite materials, prepared by standard insertion of resin into prefabricated moulds, were divided into 2 groups (n = 10 per group). On the first group, the resin composite materials were inserted into the moulds at room temperature and cured. On the second group, the resin composite materials were pre-heated in a heating device, inserted into the moulds and immediately cured. Microhardness after curing (both immediately and after 24 hours of storage) was determined (using a 300 g load applied for 10 seconds) and averaged for 5 randomly selected points on the top and bottom surfaces of each sample. To investigate viscosity, 0.5 g samples of room temperature or preheated resin composite (n = 15 per group) were placed under a 454 g load for 45 seconds before light-curing (40 seconds). After curing, each sample was photographed and the surface area calculated. Data were analyzed by t tests or one-way analysis of variance and Tukey's test. RESULTS: Preheating the resin composites increased the microhardness and decreased the viscosity of the samples. Filtek Supreme Ultra resin composite had the highest mean microhardness, and Vit-l-escence resin composite had the lowest viscosity. CONCLUSIONS: The effects of preheating resin composites may allow easier placement of restorations and greater monomer conversion.


Assuntos
Resinas Compostas/química , Dureza , Temperatura Alta , Iluminação/instrumentação , Teste de Materiais , Propriedades de Superfície , Viscosidade
6.
J Prosthodont ; 23(6): 462-6, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24597786

RESUMO

PURPOSE: This study aimed to investigate the influence of ceramic thickness and shade on the Knoop hardness and dynamic elastic modulus of a dual-cured resin cement. MATERIALS AND METHODS: Six ceramic shades (Bleaching, A1, A2, A3, A3.5, B3) and two ceramic thicknesses (1 mm, 3 mm) were evaluated. Disk specimens (diameter: 7 mm; thickness: 2 mm) of the resin cement were light cured under a ceramic block. Light-cured specimens without the ceramic block at distances of 1 and 3 mm were also produced. The Knoop hardness number (KHN), density, and dynamic Young's moduli were determined. Statistical analysis was conducted using ANOVA and a Tukey B rank order test (p = 0.05). RESULTS: The bleaching 1-mm-thick group exhibited significantly higher dynamic Young's modulus. Lower dynamic Young's moduli were observed for the 3-mm-thick ceramic groups compared to bleaching 3-mm-thick group, and no difference was found among the other 3-mm groups. For the KHN, when A3.5 3-mm-thick was used, the KHN was significantly lower than bleaching and A1 1-mm-thick ceramic; however, no difference was exhibited between the thicknesses of the same shade. CONCLUSIONS: The dual-cured resin cement studied irradiated through the 1-mm-thick ceramic with the lightest shade (bleaching ceramic) exhibited a better elastic modulus, and there was no effect in KHN of the resin cement when light cured under different ceramic shades and thicknesses (1 and 3 mm), except when the A3.5 3-mm-thick ceramic was used. CLINICAL SIGNIFICANCE: Variolink II irradiated through ceramic with the lowest chroma exhibited the highest elastic modulus; therefore, the light activation method might not be the same for all clinical situations.


Assuntos
Cerâmica/química , Porcelana Dentária/química , Cimentos de Resina/química , Cor , Módulo de Elasticidade , Dureza , Humanos , Cura Luminosa de Adesivos Dentários , Teste de Materiais , Polimerização , Autocura de Resinas Dentárias , Estresse Mecânico , Propriedades de Superfície
7.
Adv Healthc Mater ; : e2401218, 2024 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-39036851

RESUMO

Native tissues, comprising multiple cell types and extracellular matrix components, are inherently composites. Mimicking the intricate structure, functionality, and dynamic properties of native composite tissues represents a significant frontier in biomaterials science and tissue engineering research. Biomimetic composite biomaterials combine the benefits of different components, such as polymers, ceramics, metals, and biomolecules, to create tissue-template materials that closely simulate the structure and functionality of native tissues. While the design of composite biomaterials and their in vitro testing are frequently reviewed, there is a considerable gap in whole animal studies that provides insight into the progress toward clinical translation. Herein, we provide an insightful critical review of advanced composite biomaterials applicable in several tissues. The incorporation of bioactive cues and signaling molecules into composite biomaterials to mimic the native microenvironment is discussed. Strategies for the spatiotemporal release of growth factors, cytokines, and extracellular matrix proteins are elucidated, highlighting their role in guiding cellular behavior, promoting tissue regeneration, and modulating immune responses. Advanced composite biomaterials design challenges, such as achieving optimal mechanical properties, improving long-term stability, and integrating multifunctionality into composite biomaterials and future directions, are discussed. We believe that this manuscript provides the reader with a timely perspective on composite biomaterials.

8.
J Adhes Dent ; 15(5): 461-6, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23593644

RESUMO

PURPOSE: To evaluate the microtensile bond strength (MTBS) of ceramic cemented to dentin varying the resin cement and ceramic shades. MATERIALS AND METHODS: Two VITA VM7 ceramic shades (Base Dentine 0M1 and Base Dentine 5M3) were used. A spectrophotometer was used to determine the percentage translucency of ceramic (thickness: 2.5 mm). For the MTBS test, 80 molar dentin surfaces were etched and an adhesive was applied. Forty blocks (7.2 x 7.2 x 2.5 mm) of each ceramic shade were produced and the ceramic surface was etched (10% hydrofluoric acid) for 60 s, followed by the application of silane and resin cement (A3 yellow and transparent). The blocks were cemented to dentin using either A3 or transparent cement. Specimens were photoactivated for 20 s or 40 s, stored in distilled water (37°C/24 h), and sectioned. Eight experimental groups were obtained (n = 10). Specimens were tested for MTSB using a universal testing machine. Data were statistically analyzed using ANOVA and Tukey's post-hoc tests (α <= 0.05). RESULTS: The percentage translucency of 0M1 and 5M3 ceramics were 10.06 (± 0.25)% and 1.34 (± 0.02)%, respectively. The lowest MTBS was observed for the ceramic shade 5M3. For the 0M1 ceramic, the A3 yellow cement that was photocured for 20 s exhibited the lowest MTBS, while the transparent cement that was photocured for 40 s presented the highest MTBS. CONCLUSIONS: For the 2.5-mm-thick 5M3 ceramic restorations, the MTBS of ceramic cemented to dentin significantly increased. The dual-curing cement Variolink II photocured for 40 s is not recommended for cementing the Base Dentine 5M3 feldspathic ceramic to dentin.


Assuntos
Colagem Dentária , Porcelana Dentária/química , Dentina/anatomia & histologia , Cimentos de Resina/química , Condicionamento Ácido do Dente/métodos , Silicatos de Alumínio/química , Cimentação/métodos , Cerâmica/química , Cor , Cimentos Dentários/química , Análise do Estresse Dentário/instrumentação , Humanos , Ácido Fluorídrico/química , Cura Luminosa de Adesivos Dentários , Teste de Materiais , Metacrilatos/química , Microscopia Eletrônica de Varredura , Ácidos Fosfóricos/química , Compostos de Potássio/química , Silanos/química , Espectrofotometria , Estresse Mecânico , Propriedades de Superfície , Temperatura , Resistência à Tração , Fatores de Tempo , Água/química
9.
Gen Dent ; 61(1): 36-40; quiz 41, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23302361

RESUMO

This article describes CAD/CAM technology used in dentistry and different restorative materials used in conjunction with adhesive cementation with particular attention given to the evolution of the CEREC system, as well as various ceramics developed for this system. Advantages and limitations of materials and technique are also discussed.


Assuntos
Desenho Assistido por Computador/instrumentação , Porcelana Dentária/química , Planejamento de Prótese Dentária , Restauração Dentária Permanente/métodos , Estética Dentária
10.
Compend Contin Educ Dent ; 34(1): 62-3, 65-8, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23550333

RESUMO

OBJECTIVE: This study aimed to evaluate and compare the mechanical properties of five commercial core materials using fracture toughness (FT), Knoop hardness number (KHN), diametral tensile strength (DTS), and dynamic elastic moduli (DEM). METHODS: Composite material specimens were produced (Rock Core, CosmeCore, ParaCore, MultiCore Flow, and Filtek Supreme Plus). The FT test (n = 15) was performed using notchless triangular prism (NTP) specimens. FT was determined using an Instron testing machine. KHN (n = 3) was evaluated using three indentations applied on each specimen. DTS test (n = 15) was measured using an Instron testing machine. The density. of the specimens (n = 3) was determined by water displacement method. Dynamic Young's, shear moduli, and Poisson's ratio (n = 3) were measured by an ultrasonic method. Statistical analysis was conducted using ANOVA and a Tukey B rank order test (P = 0.05). RESULTS: Rock Core presented the lowest FT values. Filtek Supreme Plus and CosmeCore exhibited significantly higher KHN values than the rest of the materials. CosmeCore had the highest DTS value, which was statistically significant only compared to Rock Core. For DEM, Filtek Supreme Plus exhibited significantly higher Young's and shear moduli than the rest of the materials (P < 0.05). CONCLUSIONS: Results demonstrated significant differences in the FT, KHN, and DTS values of the core build-up materials tested. According to the elastic behavior of the core composite materials, Rock Core had the lowest Young's values.


Assuntos
Resinas Compostas/química , Materiais Dentários/química , Algoritmos , Compostos de Bário/química , Bis-Fenol A-Glicidil Metacrilato/química , Análise do Estresse Dentário/instrumentação , Módulo de Elasticidade , Dureza , Humanos , Teste de Materiais , Fenômenos Mecânicos , Metacrilatos/química , Polietilenoglicóis/química , Ácidos Polimetacrílicos/química , Técnica para Retentor Intrarradicular , Dióxido de Silício/química , Estresse Mecânico , Resistência à Tração , Uretana/análogos & derivados , Uretana/química
11.
J Orofac Orthop ; 84(Suppl 2): 65-73, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34779866

RESUMO

PURPOSE: The aim of this study was to compare classical friction (FR) in passive self-ligating brackets (P-SLBs), active self-ligating brackets (A-SLBs) and a traditional twin bracket, in vitro, and to identify the point of initiation of bracket-archwire engagement. METHODS: Nine bracket systems of 0.022 in slot size were FR tested: 5 P­SLB systems; 4 A­SLB systems; and a control group of twin brackets with elastomeric ligatures. Single upper right central incisor brackets were mounted on a custom metal fixture for testing. Straight sections of various round and rectangular nickel-titanium (NiTi) archwires (0.016, 0.018, 0.018â€¯× 0.018, 0.020â€¯× 0.020, 0.016â€¯× 0.022, 0.017â€¯× 0.025, 0.019â€¯× 0.025, and 0.021â€¯× 0.025 in) were ligated to the bracket and peak static FR (cN) was measured with an Instron Universal Testing Machine. Ten unique tests each utilizing a new bracket and new archwire were conducted for each group in the dry state. RESULTS: FR was significantly different between control, P­SLB and A­SLB systems (P < 0.001). P­SLB groups displayed no significant differences in FR between each other, regardless of archwire size. A­SLB groups did exhibit significant differences in FR between each other depending on both the bracket system and archwire size. Each A­SLB system tested possessed a distinctly different pattern of initiation of bracket-archwire engagement. CONCLUSIONS: FR between the archwire and bracket slot differs between P­SLB and A­SLB systems, with a distinct pattern of FR and bracket-archwire engagement for each A­SLB system. Understanding the different bracket-wire interactions of SLB systems should help orthodontic clinicians to plan effective and efficient biomechanics with the bracket system of their choice.


Assuntos
Ligas Dentárias , Braquetes Ortodônticos , Desenho de Aparelho Ortodôntico , Fricção , Fios Ortodônticos , Aço Inoxidável , Teste de Materiais , Análise do Estresse Dentário , Titânio
12.
Materials (Basel) ; 17(1)2023 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-38203994

RESUMO

In recent years, the field of mandibular reconstruction has made great strides in terms of hardware innovations and their clinical applications. There has been considerable interest in using computer-aided design, finite element modelling, and additive manufacturing techniques to build patient-specific surgical implants. Moreover, lattice implants can mimic mandibular bone's mechanical and structural properties. This article reviews current approaches for mandibular reconstruction, their applications, and their drawbacks. Then, we discuss the potential of mandibular devices with lattice structures, their development and applications, and the challenges for their use in clinical settings.

13.
Int J Prosthodont ; 0(0): 0, 2023 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-37196037

RESUMO

PURPOSE: To evaluate the effect of restoration design on fracture resistance and stress distribution of veneered and monolithic 3-unit zirconia fixed partial dentures (FDPs) using finite element analysis (FEA). MATERIALS AND METHODS: Identical epoxy resin replicas of mandibular second premolar and second molar (to serve as abutment for the 3-unit bridge) were divided into four groups (n = 10): monolithic zirconia (MZ) restorations; conventional layering veneering technique (ZL), heat-pressed technique (ZP), or CAD/CAM lithium disilicate glass ceramic (CAD-on). Specimens were subjected to compressive cyclic loading on the mesio-buccal cusp of the pontic (load range 50 to 600 N; aqueous environment; 500,000 cycles) in a universal testing machine. Data were statistically analyzed at 5% significance level with Fisher exact test and Kaplan-Meier survival analysis. 3D models were constructed in accordance with experimental groups. The stress distribution in each model was analyzed and evaluated according to the location and magnitude of the maximum principal stresses (MPS) using ANSYS software. RESULTS: Specimens from ZL and ZP groups failed at different stages of the 500,000 cycles fatigue, while CAD-on and MZ restorations survived fatigue test. Statistically, there was a significant difference between the groups (P < .001). The MPS were located under the mesial connector in both monolithic and bilayered 3-unit zirconia FDPs. These stresses were found to be higher in monolithic geometries compared to bilayered zirconia FDPs. CONCLUSION: Monolithic 3-unit zirconia and CAD-on zirconia frameworks resulted in superior fracture resistance. Restoration design significantly affected the stress distribution of 3-unit zirconia FDPs.

14.
Gels ; 9(12)2023 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-38131909

RESUMO

Hydrogels are three-dimensional (3D) water-swellable polymeric matrices that are used extensively in tissue engineering and drug delivery. Hydrogels can be conformed into any desirable shape using 3D bio-printing, making them suitable for personalized treatment. Among the different 3D bio-printing techniques, digital light processing (DLP)-based printing offers the advantage of quickly fabricating high resolution structures, reducing the chances of cell damage during the printing process. Here, we have used DLP to 3D bio-print biocompatible gelatin methacrylate (GelMA) scaffolds intended for bone repair. GelMA is biocompatible, biodegradable, has integrin binding motifs that promote cell adhesion, and can be crosslinked easily to form hydrogels. However, GelMA on its own is incapable of promoting bone repair and must be supplemented with pharmaceutical molecules or growth factors, which can be toxic or expensive. To overcome this limitation, we introduced zinc-based metal-organic framework (MOF) nanoparticles into GelMA that can promote osteogenic differentiation, providing safer and more affordable alternatives to traditional methods. Incorporation of this nanoparticle into GelMA hydrogel has demonstrated significant improvement across multiple aspects, including bio-printability, and favorable mechanical properties (showing a significant increase in the compressive modulus from 52.14 ± 19.42 kPa to 128.13 ± 19.46 kPa with the addition of ZIF-8 nanoparticles). The designed nanocomposite hydrogels can also sustain drug (vancomycin) release (maximum 87.52 ± 1.6% cumulative amount) and exhibit a remarkable ability to differentiate human adipose-derived mesenchymal stem cells toward the osteogenic lineage. Furthermore, the formulated MOF-integrated nanocomposite hydrogel offers the unique capability to coat metallic implants intended for bone healing. Overall, the remarkable printability and coating ability displayed by the nanocomposite hydrogel presents itself as a promising candidate for drug delivery, cell delivery and bone tissue engineering applications.

15.
Heliyon ; 9(7): e17787, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37449193

RESUMO

Objective: To evaluate the mechanical properties of different CAD/CAM ceramic systems and the post-fatigue fracture and stress distribution when used as cemented crowns. Materials and methods: Sixty (60) CAD/CAM monolithic crowns were milled using three different ceramic materials (FD - Feldspathic [Vita Mark II]), LE - Leucite-based ceramic [IPS Empress CAD] and LD - Lithium Disilicate [IPS e.max CAD]) and adhesively cemented on resin composite dyes. Specimens were stored in distillated water (37 °C) for 7 days. After, half of the crowns were submitted to immediate fracture load test while the other half was submitted to fatigue cycling. The average cement layer of approximately 80 µm was assessed using scanning electron microscopy (SEM). The average thickness was used in the three-dimensional (3D) Finite Element Analysis (FEA). For each ceramic material, the density, Poisson ratio, shear modulus, Young modulus, fracture toughness, and true hardness were assessed (n = 3). The data was used to assess the Maximum Principal Stress throughout 3D-FEA according to each material during load to fail and post-fatigue. Data were submitted to two-way ANOVA and Tukey test (α = 0.05). Results: LD showed the highest compression load, density, shear modulus, Young modulus, fracture toughness and true hardness values. While LE presented the lowest mechanical properties values. There is no difference in the Poisson ratio between the evaluated ceramics. Conclusion: LD was susceptible to aging process but presented stronger physicomechanical properties, showing the highest post-fatigue fracture load and highest stress magnitude.

16.
Am J Pathol ; 179(5): 2189-98, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21907695

RESUMO

Efficient deposition of type I collagen is fundamental to healing after myocardial infarction. Whether there is also a role for cleavage of type I collagen in infarct healing is unknown. To test this, we undertook coronary artery occlusion in mice with a targeted mutation (Col1a1(r/r)) that yields collagenase-resistant type I collagen. Eleven days after infarction, Col1a1(r/r) mice had a lower mean arterial pressure and peak left ventricular systolic pressure, reduced ventricular systolic function, and worse diastolic function, compared with wild-type littermates. Infarcted Col1a1(r/r) mice also had greater 30-day mortality, larger left ventricular lumens, and thinner infarct walls. Interestingly, the collagen fibril content within infarcts of mutant mice was not increased. However, circular polarization microscopy revealed impaired collagen fibril organization and mechanical testing indicated a predisposition to scar microdisruption. Three-dimensional lattices of collagenase-resistant fibrils underwent cell-mediated contraction, but the fibrils did not organize into birefringent collagen bundles. In addition, time-lapse microscopy revealed that, although cells migrated smoothly on wild-type collagen fibrils, crawling and repositioning on collagenase-resistant collagen was impaired. We conclude that type I collagen cleavage is required for efficient healing of myocardial infarcts and is critical for both dynamic positioning of collagen-producing cells and hierarchical assembly of collagen fibrils. This seemingly paradoxical requirement for collagen cleavage in fibrotic repair should be considered when designing potential strategies to inhibit matrix degradation in cardiac disease.


Assuntos
Colágeno Tipo I/metabolismo , Colagenases/fisiologia , Fibroblastos/enzimologia , Infarto do Miocárdio/enzimologia , Cicatrização/fisiologia , Animais , Movimento Celular , Colágeno Tipo I/genética , Cadeia alfa 1 do Colágeno Tipo I , Colagenases/genética , Constrição , Vasos Coronários , Fibroblastos/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mutação/genética
17.
Langmuir ; 28(8): 3871-80, 2012 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-22242934

RESUMO

The behavior of cells responsible for bone formation, osseointegration, and bone bonding in vivo are governed by both the surface chemistry and topography of scaffold matrices. Bone-like apatite coatings represent a promising method to improve the osteoconductivity and bonding of synthetic scaffold materials to mineralized tissues for regenerative procedures in orthopedics and dentistry. Polycaprolactone (PCL) films were coated with calcium phosphates (CaP) by incubation in simulated body fluid (SBF). We investigated the effect of SBF ion concentration and soaking time on the surface properties of the resulting apatite coatings. CaP coatings were examined by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectrometry (FTIR), and energy dispersive X-ray spectrometry (EDX). Young's modulus (E(s)) was determined by nanoindentation, and surface roughness was assessed by atomic force microscopy (AFM) and mechanical stylus profilometry. CaP such as carbonate-substituted apatite were deposited onto PCL films. SEM and AFM images of the apatite coatings revealed an increase in topographical complexity and surface roughness with increasing ion concentration of SBF solutions. Young's moduli (E(s)) of various CaP coatings were not significantly different, regardless of the CaP phase or surface roughness. Thus, SBF with high ion concentrations may be used to coat synthetic polymers with CaP layers of different surface topography and roughness to improve the osteoconductivity and bone-bonding ability of the scaffold.


Assuntos
Materiais Biocompatíveis/química , Biomimética/métodos , Fosfatos de Cálcio/química , Materiais Revestidos Biocompatíveis/química , Módulo de Elasticidade , Microscopia Eletrônica de Varredura , Poliésteres/química , Espectrometria por Raios X , Propriedades de Superfície , Difração de Raios X
18.
Gen Dent ; 60(4): e215-20, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22782054

RESUMO

The aim of this study was to determine how resin cement, self-adhesive resin cement, and resin-modified glass ionomer cement affected shear bond strength to dentin. Sixty composite resin disks (3 mm in diameter x 3 mm in length) were prepared and divided into four groups (n = 15): Group 1, composite disk bonded to dentin with composite resin and a bonding agent; Group 2, composite disk bonded to dentin with a self-adhesive resin cement; Group 3, composite disk bonded to dentin with a different self-adhesive resin cement; and Group 4, composite disk bonded to dentin with a resin-modified glass ionomer cement. The composite resin was loaded into a syringe (internal diameter 3 mm), photocured in an oven, and cut into 3 mm slices with a low-speed saw. The samples were bonded to dentin per the manufacturer's instructions. All specimens were stored in distilled water (at 37 degrees C) for 24 hours. The shear bond strength test was conducted using a universal testing machine at a crosshead speed of 0.5 mm/min until failure. Conventional resin cement and a bonding agent exhibited significantly higher shear bond strength values than all other materials tested.


Assuntos
Colagem Dentária , Dentina/ultraestrutura , Cimentos de Resina/química , Condicionamento Ácido do Dente/métodos , Adesividade , Bis-Fenol A-Glicidil Metacrilato/química , Resinas Compostas/química , Corrosão Dentária/métodos , Materiais Dentários/química , Análise do Estresse Dentário/instrumentação , Cimentos de Ionômeros de Vidro/química , Humanos , Teste de Materiais , Polietilenoglicóis/química , Polimerização , Ácidos Polimetacrílicos/química , Cimentos de Resina/classificação , Resistência ao Cisalhamento , Estresse Mecânico , Propriedades de Superfície , Temperatura , Fatores de Tempo , Água/química
19.
Gen Dent ; 59(6): e219-23, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22313926

RESUMO

This study evaluated the diametral tensile strength of composite resin core materials with and without fiber dowels. Eight groups were established (n = 20), four with composite resins and four with fiber dowels. Samples were tested using a universal testing machine and evaluated using scanning electron microscopy. One-way ANOVA and a Tukey B-rank order test (P = 0.05) indicated that the tensile values of two of the four composite resins decreased significantly when their matching fiber dowels were introduced.


Assuntos
Resinas Compostas/química , Materiais Dentários/química , Técnica para Retentor Intrarradicular/instrumentação , Falha de Restauração Dentária , Análise do Estresse Dentário/instrumentação , Humanos , Microscopia Eletrônica de Varredura , Cimentos de Resina/química , Estresse Mecânico , Propriedades de Superfície , Resistência à Tração
20.
Gen Dent ; 59(2): 125-8; quiz 129-30, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21903522

RESUMO

The aim of this study was to determine the influence of different types of posts and post head designs on the fracture resistance of a composite resin core material using the diametral tensile strength (DTS ). Seventy-five disc specimens were prepared using a composite core and prefabricated glass fiber posts and were divided into four test groups and one control group (n=15). The use of fiber posts reduced the DTS of the composite core material; the DTS value of the control material was significantly higher (p=0.05) than all of the test groups.


Assuntos
Resinas Compostas/química , Materiais Dentários/química , Vidro/química , Técnica para Retentor Intrarradicular/instrumentação , Planejamento de Prótese Dentária , Análise do Estresse Dentário/instrumentação , Humanos , Teste de Materiais , Processos Fotoquímicos , Polimerização , Polímeros/química , Estresse Mecânico , Temperatura , Resistência à Tração , Fatores de Tempo , Água/química
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