Assessing the immediate impact of COVID-19 lockdown on the air quality of Kolkata and Howrah, West Bengal, India.

The worldwide spread of COVID-19 caused a nationwide lockdown in India from 24 March 2020 and was further extended up to 3 May 2020 to break off the transmission of novel Coronavirus. The study is designed to assess the changes in air quality from the pre-lockdown period to the during lockdown period in Kolkata and Howrah municipal corporation, West Bengal, India. GIS-based techniques include the spatial and temporal distribution of pollutants using interpolation method, and on the other hand, statistical methods like analysis of variance (ANOVA) was applied to determine the mean differences two phases and correlation matrix helps to understand the changing association of the pollutants in pre- and during lockdown phases. Significant correlations have been found among the pollutants, ANOVA (Two-Way) has shown the significant mean difference of NAQI between the two phases, F(1,611) = 465.723, p < 0.0001; pairwise comparison for Ballygunge has shown the highest mean difference 108.194 at p < 0.0001 significant level between lockdown and pre-lockdown phase. Significant positive correlation has been found between PM2.5, PM10 (0.99*); PM2.5, NO2 (0.81*); PM10, NO2 (0.81*); CO, NO2 (0.77*) and some negative correlations have also been found between O3, NO (- 0.15); O3 and NH3 (- 0.36) in the pre-lockdown phase. The reduction amount of mean concentration from the pre-lockdown phase to during lockdown of the main pollutants like PM2.5, PM10 and NO2 are ~ 58.71%, ~ 57.92% and ~ 55.23%. Near Rabindra Bharati University constant emission of PM2.5, 10 and NO2 have been recorded due to the nearby Cossipore thermal power station.

Neuronal expression of Mgat1 rescues the shortened life span of Drosophila Mgat11 null mutants and increases life span.

The enzyme UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I (GnT1, encoded by Mgat1) controls the synthesis of paucimannose N-glycans in Drosophila. We have previously reported that null mutations in Drosophila Mgat1 are viable but exhibit defects in locomotion, brain abnormalities, and a severely reduced life span. Here, we show that knockdown of Mgat1 in the central nervous system (CNS) of wild-type flies decreases locomotor activity and life span. This phenotype is similar to that observed in Drosophila Mgat1(1) null mutants, demonstrating that Mgat1 is required in the CNS. We also found that neuronal expression of a wild-type Mgat1 transgene rescued the shortened life span of Mgat1(1) null mutants and resulted in a dramatic 135% increase in mean life span relative to genetically identical controls. Neuronal expression of a wild-type Mgat1 transgene in wild-type flies resulted in a modest 9% increase in mean life span relative to genetically identical controls. In both Mgat1(1) null mutants and wild-type flies, neuronal expression of wild-type Mgat1 transgene resulted in a significant increase in GnT1 activity and resistance to oxidative stress. Whereas dietary restriction is not absolutely essential for the increased life span, it plays a role in the process. Interestingly, we observe a direct correlation between GnT1 activity and mean life span up to a maximum of appropriately 136 days, showing that the ability of GnT1 activity to increase life span is limited. Altogether, these observations suggest that Mgat1-dependent N-glycosylation plays an important role in the control of Drosophila life span.

Assessing the impact of precipitation on hardrock aquifer system using standard precipitation index and groundwater resilience index: a case study of Purulia, West Bengal, India.

Groundwater stored in the aquifers provides water security during natural hazards, e.g. clean water access during floods and droughts. Groundwater drought, a phenomenon closely linked with rainfall (climate) variability, is less researched, especially in India. This study aims to detect precipitation and groundwater droughts and comprehend the groundwater response to long-term precipitation trends (25 years). As a case study, the drought-affected and groundwater-depleted Purulia district in West Bengal, India, which is a part of the Chotanagpur plateau, was selected. Precipitation and groundwater droughts (in aquifer types of shallow, moderate and deep) are detected using the Standard Precipitation Index (SPI) and Groundwater Resilience Index (GRI). During the 25 year study period (1996-2020), Purulia had 13 (52%) rainfall deficiency years, with an annual average rainfall of 1382 mm. SPI detected four severe droughts and the most severe occurring in 2010-2011 (1.50). GRI found that aquifermedium had a 71% [Formula: see text] conditions and are the most resilient and aquiferdeep experienced maximum extreme drought events and is the most stressed. The cross-correlation coefficients (CCCs) between rainfall and groundwater is moderate in deep, shallow, and medium aquifers, with CCCs - 0.43, - 0.59, and - 0.49, respectively. Positive CCCs are found for seasonal lags of - 3, - 4, and - 7. The study found that during the monsoon, average depth to groundwater level is 1 - 4 m and it drops to 8 - 10 m during the lean period, more than 85% of wells are vulnerable to extreme droughts (SPI > 1.5), aquifer's response to rainfall is aquifershallow > aquifermoderate > aquiferdeep, and aquifer's may be arranged as aquifermoderate > aquifershallow > aquiferdeep depending on their drought resistance. This study, with the use of statistical tools and long term data, will aid in the management of groundwater at varying depths by creating basis for understanding the groundwater response to rainfall events.

Null mutations in Drosophila N-acetylglucosaminyltransferase I produce defects in locomotion and a reduced life span.

UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I (encoded by Mgat1) controls the synthesis of hybrid, complex, and paucimannose N-glycans. Mice make hybrid and complex N-glycans but little or no paucimannose N-glycans. In contrast, Drosophila melanogaster and Caenorhabditis elegans make paucimannose N-glycans but little or no hybrid or complex N-glycans. To determine the functional requirement for beta1,2-N-acetylglucosaminyltransferase I in Drosophila, we generated null mutations by imprecise excision of a nearby transposable element. Extracts from Mgat1(1)/Mgat1(1) null mutants showed no beta1,2-N-acetylglucosaminyltransferase I enzyme activity. Moreover, mass spectrometric analysis of these extracts showed dramatic changes in N-glycans compatible with lack of beta1,2-N-acetylglucosaminyltransferase I activity. Interestingly, Mgat1(1)/Mgat1(1) null mutants are viable but exhibit pronounced defects in adult locomotory activity when compared with Mgat1(1)/CyO-GFP heterozygotes or wild type flies. In addition, in null mutants males are sterile and have a severely reduced mean and maximum life span. Microscopic examination of mutant adult fly brains showed the presence of fused beta lobes. The removal of both maternal and zygotic Mgat1 also gave rise to embryos that no longer express the horseradish peroxidase antigen within the central nervous system. Taken together, the data indicate that beta1,2-N-acetylglucosaminyltransferase I-dependent N-glycans are required for locomotory activity, life span, and brain development in Drosophila.