Evaluation of acid-phase digestion as a pretreatment to enhance co-digestion of source separated organics and municipal sewage sludges.

This work assessed if acid-phase digestion could improve volatile solids (VS) destruction and methane yield when co-digesting municipal sewage sludges (primary and waste activated sludge) and source separated organics (SSO). The SSO was made up of food waste and the organic fraction of municipal solid waste. Two laboratory-scale acid-phase digesters and three laboratory-scale methane-phase digesters were employed in order to determine the impacts of SSO co-digestion with municipal sludges both with and without acid-phase digestion as a pretreatment step. Reactors were operated at 35 °C using volatile solids loading rates of 34.2-44.1 g VS/LR-day for acid-phase digesters and 1.2-2.4 1 g VS/LR-day for methane-phase digesters. Solids retention times ranging from 1.2 to 1.5 day and 20.7 to 23.2 days were employed for acid-phase and methane-phase digesters, respectively. VS destruction ranged from 62% to 67%, with reactors receiving SSO achieving higher VS destruction. Results also show that reactors receiving SSO were able to handle organic loading increases of at least 39% without showing signs of overloading. Microbial community analysis revealed that SSO had a noticeable impact on acid-phase digestion with Megasphaera emerging as the most abundant genus.

Influent wastewater microbiota and temperature influence anaerobic membrane bioreactor microbial community.

Sustainable municipal wastewater recovery scenarios highlight benefits of anaerobic membrane bioreactors (AnMBRs). However, influences of continuous seeding by influent wastewater and temperature on attached-growth AnMBRs are not well understood. In this study, four bench-scale AnMBR operated at 10 and 25°C were fed synthetic (SPE) and then real (PE) primary effluent municipal wastewater. Illumina sequencing revealed different bacterial communities in each AnMBR in response to temperature and bioreactor configuration, whereas differences were not observed in archaeal communities. Activity assays revealed hydrogenotrophic methanogenesis was the dominant methanogenic pathway at 10°C. The significant relative abundance of Methanosaeta at 10°C concomitant with low acetoclastic methanogenic activity may indicate possible Methanosaeta-Geobacter direct interspecies electron transfer. When AnMBR feed was changed to PE, continual seeding with wastewater microbiota caused AnMBR microbial communities to shift, becoming more similar to PE microbiota. Therefore, influent wastewater microbiota, temperature and reactor configuration influenced the AnMBR microbial community.