[Study of the genetic markers of thrombosis in hypertensive patients of the native population in the Republic of Mordovia].

The genetic markers (encoding factor of blood coagulation, the PAI-1 gene, the prothrombin gene, the GP IIb/IIIa gene, and the MTHFR gene) of thrombosis were studied in the Mordovians and Russians with essential hypertension (EH), who were residents of the Republic of Mordovia. One hundred and forty patients with TH and 90 healthy individuals of three nationalities, such as Russian, Moksha-Mordovian, and Erzya-Mordovian, were examined. Along with the routine clinical and instrumental studies, alleles of polymorphic markers were identified by the polymerase chain reaction (PCR). The polymorphic markers of the Arg 506 Gln gene, encoding factor V of blood coagulation, 4G5G of the PAI-1 gene, the G 20210 A prothrombin gene, the GP IIb/IIIa gene, and the A 1298 C MTHFR gene were also studied. The data were statistically analyzed using a package of the programs: Statistica for Windows 6.0 (Stat Soft), SPSS (version 14.0), and MS Excel XP (Microsoft). The authors used a chi-2 (chi-square) test was used to compare the incidence of genotypes and alleles in the patient groups. Hypertensive and normotensive persons were found to have no significant differences in the distribution of genotypes (encoding factor of blood coagulation, the PAI-1 gene, the prothrombin gene, and the GP IIb/IIIa gene) with regard to ethnicity in the Russian, and Moksha-Mordovian, and Erzya-Mordovian groups). Meanwhile, there was a significant preponderance of MTHFR gene mutation with the unfavorable genotype for Moksha-Mordovian patients with EH. The findings suggest that drug therapy for preventing venous thrombosis in patients with EH should be adjusted in relation to ethnicity.

[Possibilities of treatment optimization in children and adolescents with epilepsy and disturbances of emotion and volition (disphoria)]. / Vozmozhnosti optimizatsii terapii detei i podrostkov, stradayushchikh epilepsiei s emotsional'no-volevymi narusheniyami (disforii).

AIM: To optimize the treatment of dysphoriain children and adolescents in regard to sex and disease severity. MATERIAL AND METHODS: Seventy children and adolescents (boys - 45, girls - 25), aged from 6 to 18 years, with different forms of epilepsy and emotion and dysphoric disturbances were studied using CPRS andGCIscales Depending on dysphoria severity, patients were stratified into three groups: mild (n=19 (27.1%), moderate (n=27 (38.6%)) and severe (n=24 (34.3%)). RESULTS AND CONCLUSION: Dysphoric disorders were significantly more prevalent in boys, hostility and aggression were characteristic of boys as well. These facts impactedtreatment options. Neuroleptics were more frequently used in boys (35.5%) compared to girls(16%).Mild dysphoria didn't require additional treatment besides AED in 78,4%. In 75% cases of moderate dysphoria,systemic treatment with neuroleptics for 6 months was necessary. One-time recommendations for neuroleptic treatment were made in all three groups with the prevalence in a groupof children with severe and moderate dysphoria.

[The isolation and characteristics of continuous virus-producing lymphoid suspension cultures of African green monkey cells]. / Poluchenie i kharakteristika perevivaemykh virusprodutsiruiushchikh limfoidnykh suspenzionnykh kul'tur kletok afrikanskikh zelenykh martyshek.

Five continuous lymphoid suspension cell cultures were established by cultivation of lymphocytes from African green monkeys. The immunological typing revealed the mixed pattern of cultures consisting of B and T cell types. Two types of viruses: herpesvirus and retrovirus C-type were demonstrated by electron microscopy. Indirect immunofluorescence and molecular hybridization studies showed one of the viruses to be an EBV-related herpesvirus of green monkeys designated HVGM and the other to belong to H (S) TLV-1 group.

[Disease characteristics of the population on the territory of the city of Kirovo-Chepetsk]. / Kharakteristika zabolivaemosti naseleniia na territorii g. Kirovo- Chepetska.

Medical and ecologic evaluation of ecologic situation in Kirovo-Tchepetsk (Kirov region) included study of morbidity in the town and referent territories. Levels and dynamics of morbidity, prevalence, number of pathologic processes in children and other parameters could characterize the town as an ecologically unfavorable territory.

[The microcirculatory reorganization of the lesser circulation during the rehabilitation of myocardial infarct patients]. / Perestroika mikrotsirkuliatsii malogo kruga v protsesse reabilitatsii bol'nykh infarktom miokarda.

A study was made of the regional distribution of the pulmonary blood flow by means of perfusion scintigraphy of the lungs with 99mTc-albumin macroaggregates with relation to a clinical course of CHD, the state of intracardiac and pulmonary hemodynamics. The time course of microcirculation in the lesser blood flow during rehabilitation of patients after myocardial infarction was assessed. Redistribution of the pulmonary blood flow was shown to be connected with the clinical features of a course of CHD, severity of myocardial lesion, and a degree of disorder of intracardiac and pulmonary hemodynamics. Therefore these changes can be of important prognostic value in patients after myocardial infarction.

[Comparative analysis of genomes of oncogenic B-lymphotropic herpesviruses reproducing in cell lines obtained from different monkey species]. / Sravnitel'nyi analiz genomov onkogennykh B-limfotropnykh virusov gerpesa, reprodutsiruiushchikhsia v kletochnykh liniiakh, poluchennykh ot obez'ian razlichnykh vidov.

The aim of this work was to study genomes of B-lymphotropic oncogenic herpesviruses of Macaca arctoides (HVMA), Papio hamadryas (HVP) and Cercopithecus aethiops (HVGM). Blot-hybridization detected inter- and intraspecific genome heterogeneity of investigated viruses in restriction sites EcoR1, Pst1 and Hind111. Virus-specific sequences in two-cell lines obtained from HVMA-induced rabbit tumours can prove HVMA a role in malignant tumour induction in rabbits.