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1.
Diabetologia ; 67(4): 738-754, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38236410

RESUMO

AIMS/HYPOTHESIS: Sodium-glucose co-transporter 2 (SGLT2) inhibitors (SGLT2i) are antihyperglycaemic drugs that protect the kidneys of individuals with type 2 diabetes mellitus. However, the underlying mechanisms mediating the renal benefits of SGLT2i are not fully understood. Considering the fuel switches that occur during therapeutic SGLT2 inhibition, we hypothesised that SGLT2i induce fasting-like and aestivation-like metabolic patterns, both of which contribute to the regulation of metabolic reprogramming in diabetic kidney disease (DKD). METHODS: Untargeted and targeted metabolomics assays were performed on plasma samples from participants with type 2 diabetes and kidney disease (n=35, 11 women) receiving canagliflozin (CANA) 100 mg/day at baseline and 12 week follow-up. Next, a systematic snapshot of the effect of CANA on key metabolites and pathways in the kidney was obtained using db/db mice. Moreover, the effects of glycine supplementation in db/db mice and human proximal tubular epithelial cells (human kidney-2 [HK-2]) cells were studied. RESULTS: Treatment of DKD patients with CANA for 12 weeks significantly reduced HbA1c from a median (interquartile range 25-75%) of 49.0 (44.0-57.0) mmol/mol (7.9%, [7.10-9.20%]) to 42.2 (39.7-47.7) mmol/mol (6.8%, [6.40-7.70%]), and reduced urinary albumin/creatinine ratio from 67.8 (45.9-159.0) mg/mmol to 47.0 (26.0-93.6) mg/mmol. The untargeted metabolomics assay showed downregulated glycolysis and upregulated fatty acid oxidation. The targeted metabolomics assay revealed significant upregulation of glycine. The kidneys of db/db mice undergo significant metabolic reprogramming, with changes in sugar, lipid and amino acid metabolism; CANA regulated the metabolic reprogramming in the kidneys of db/db mice. In particular, the pathways for glycine, serine and threonine metabolism, as well as the metabolite of glycine, were significantly upregulated in CANA-treated kidneys. Glycine supplementation ameliorated renal lesions in db/db mice by inhibiting food intake, improving insulin sensitivity and reducing blood glucose levels. Glycine supplementation improved apoptosis of human proximal tubule cells via the AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway. CONCLUSIONS/INTERPRETATION: In conclusion, our study shows that CANA ameliorates DKD by inducing fasting-like and aestivation-like metabolic patterns. Furthermore, DKD was ameliorated by glycine supplementation, and the beneficial effects of glycine were probably due to the activation of the AMPK/mTOR pathway.


Assuntos
Diabetes Mellitus Tipo 2 , Nefropatias Diabéticas , Inibidores do Transportador 2 de Sódio-Glicose , Camundongos , Animais , Humanos , Feminino , Canagliflozina/farmacologia , Canagliflozina/uso terapêutico , Diabetes Mellitus Tipo 2/metabolismo , Nefropatias Diabéticas/metabolismo , Reprogramação Metabólica , Proteínas Quinases Ativadas por AMP/metabolismo , Transportador 2 de Glucose-Sódio/metabolismo , Estivação , Inibidores do Transportador 2 de Sódio-Glicose/farmacologia , Inibidores do Transportador 2 de Sódio-Glicose/uso terapêutico , Inibidores do Transportador 2 de Sódio-Glicose/metabolismo , Rim/metabolismo , Jejum , Serina-Treonina Quinases TOR/metabolismo , Glicina/metabolismo , Mamíferos/metabolismo
2.
Mol Med ; 30(1): 71, 2024 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-38797859

RESUMO

Diabetes mellitus, a chronic metabolic disease, often leads to numerous chronic complications, significantly contributing to global morbidity and mortality rates. High glucose levels trigger epigenetic modifications linked to pathophysiological processes like inflammation, immunity, oxidative stress, mitochondrial dysfunction, senescence and various kinds of cell death. Despite glycemic control, transient hyperglycemia can persistently harm organs, tissues, and cells, a latent effect termed "metabolic memory" that contributes to chronic diabetic complications. Understanding metabolic memory's mechanisms could offer a new approach to mitigating these complications. However, key molecules and networks underlying metabolic memory remain incompletely understood. This review traces the history of metabolic memory research, highlights its key features, discusses recent molecules involved in its mechanisms, and summarizes confirmed and potential therapeutic compounds. Additionally, we outline in vitro and in vivo models of metabolic memory. We hope this work will inform future research on metabolic memory's regulatory mechanisms and facilitate the development of effective therapeutic compounds to prevent diabetic complications.


Assuntos
Complicações do Diabetes , Humanos , Animais , Complicações do Diabetes/metabolismo , Diabetes Mellitus/metabolismo , Diabetes Mellitus/etiologia , Epigênese Genética , Estresse Oxidativo , Hiperglicemia/metabolismo
3.
FASEB J ; 37(6): e22938, 2023 06.
Artigo em Inglês | MEDLINE | ID: mdl-37130011

RESUMO

Diabetic kidney disease (DKD) develops in ~40% of patients with diabetes and is the leading cause of chronic kidney disease worldwide. We used single-cell RNA-sequencing and spatial transcriptomic analyses of kidney specimens from patients with DKD. Unsupervised clustering revealed distinct cell clusters, including epithelial cells and fibroblasts. We also identified differentially expressed genes (DEGs) and assessed enrichment, and cell-cell interactions. Specific enrichment of DKD was evident in venous endothelial cells (VECs) and fibroblasts with elevated CCL19 expression. The DEGs in most kidney parenchymal cells in DKD were primarily enriched in inflammatory signaling pathways. Intercellular crosstalk revealed that most cell interactions in DKD are associated with chemokines. Spatial transcriptomics revealed that VECs co-localized with fibroblasts, with most immune cells being enriched in areas of renal fibrosis. These results provided insight into the cell populations, intercellular interactions, and signaling pathways underlying the pathogenesis and potential targets for treating DKD.


Assuntos
Diabetes Mellitus , Nefropatias Diabéticas , Humanos , Nefropatias Diabéticas/metabolismo , Células Endoteliais/metabolismo , Transcriptoma , Análise da Expressão Gênica de Célula Única , Rim/metabolismo , Diabetes Mellitus/metabolismo
4.
Appl Opt ; 63(10): 2621-2629, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38568545

RESUMO

When a binocular vision sensor (BVS) is installed in a narrow space, traditional calibration methods are limited as all targets should be placed in more than three different positions. To solve this problem, an on-site calibration method based on the phase-shift algorithm is proposed in our paper. Intrinsic parameters of these two cameras should be first calibrated offline. Series of phase-shift patterns are projected onto any one target with known three-dimensional information to determine the relationship between two cameras. The target utilized in our proposed method can be selected arbitrarily, which is suitable to achieve the on-site calibration of BVS, especially in industrial vibration environments. Experiments are conducted to validate the effectiveness and robustness of our proposed method.

5.
J Cell Physiol ; 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36790954

RESUMO

Bacillus species act as plant growth-promoting rhizobacteria (PGPR) that can produce a large number of bioactive metabolites. Bacillaene, a linear polyketide/nonribosomal peptide produced by Bacillus strains, is synthesized by the trans-acyltransferase polyketide synthetase. The complexity of the chemical structure, particularity of biosynthesis, potent bioactivity, and the important role of competition make Bacillus an ideal antibiotic weapon to resist other microbes and maintain the optimal rhizosphere environment. This review provides an updated view of the structural features, biological activity, biosynthetic regulators of biosynthetic pathways, and the important competitive role of bacillaene during Bacillus survival.

6.
Plant Dis ; 107(8): 2325-2334, 2023 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-37596715

RESUMO

Banana (Musa spp.) is an important fruit and food crop worldwide. In recent years, banana sheath rot has become a major problem in banana cultivation, causing plant death and substantial economic losses. Nevertheless, the pathogen profile of this disease has not been fully characterized. Klebsiella variicola is a versatile bacterium capable of colonizing different hosts, such as plants, humans, insects, and animals, and is recognized as an emerging pathogen in various hosts. In this study, we obtained 12 bacterial isolates from 12 different banana samples showing banana sheath rot in Guangdong and Guangxi Provinces, China. Phylogenetic analysis based on 16S rRNA sequences confirmed that all 12 isolates were K. variicola strains. We sequenced the genomes of these strains, performed comparative genomic analysis with other sequenced K. variicola strains, and found a lack of consistency in accessory gene content among these K. variicola strains. However, prediction based on the pan-genome of K. variicola revealed 22 unique virulence factors carried by the 12 pathogenic K. variicola isolates. Microbiome and microbial interaction network analysis of endophytes between the healthy tissues of diseased plants and healthy plants of two cultivars showed that Methanobacterium negatively interacts with Klebsiella in banana plants and that Herbaspirillum might indirectly inhibit Methanobacterium to promote Klebsiella growth. These results suggest that banana sheath rot is caused by the imbalance of plant endophytes and opportunistic pathogenic bacteria, providing an important basis for research and control of this disease.[Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.


Assuntos
Musa , Animais , Humanos , Filogenia , RNA Ribossômico 16S/genética , China , Klebsiella/genética , Endófitos
7.
Lab Invest ; 101(11): 1484-1493, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34446806

RESUMO

Leydig cells (LCs) apoptosis is responsible for the deficiency of serum testosterone in Late-onset hypogonadism (LOH), while its specific mechanism is still unknown. This study focuses on the role of long noncoding RNA (lncRNA) MIR22HG in LC apoptosis and aims to elaborate its regulatory mechanism. MIR22HG was up-regulated in the testicular tissues of mice with LOH and H2O2-treated TM3 cells (mouse Leydig cell line). Interference of MIR22HG ameliorated cell apoptosis and upregulated miR-125a-5p expression in H2O2-treated TM3 cells. Then, the interaction between MIR22HG and miR-125a-5p was confirmed with RIP and RNA pull-down assay. Further study showed that miR-125a-5p downregulated N-Myc downstream-regulated gene 2 (NDRG2) expression by targeting its 3'-UTR of mRNA. What's more, MIR22HG overexpression aggravated cell apoptosis and reduced testosterone production in TM3 cells via miR-125a-5p/NDRG2 pathway. MIR22HG knockdown elevated testosterone levels in LOH mice. In conclusion, MIR22HG up-regulated NDRG2 expression through targeting miR-125a-5p, thus promoting LC apoptosis in LOH.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Hipogonadismo/etiologia , Células Intersticiais do Testículo/fisiologia , MicroRNAs/metabolismo , MicroRNAs/fisiologia , Animais , Apoptose , Linhagem Celular , Masculino , Camundongos , Testosterona/metabolismo
8.
J Cell Physiol ; 235(2): 1001-1012, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31240707

RESUMO

Emerging evidence has indicated that long noncoding RNA (lncRNAs) play crucial roles in regulating thyroid cancer (TC) development. Linc00210 is a newly identified lncRNA which plays an oncogenic role in hepatocellular carcinoma and nasopharyngeal carcinoma, but whether Linc00210 can modulate the development of TC remains elusive. Here, we found that Linc00210 expression was upregulated in TC tissues compared to the matched noncancerous tissues. Overexpression of Linc00210 augmented the proliferation, migration, and invasion of TC cells. Mechanistically, Linc00210 served as a sponge for miR-195-5p, thereby counteracting its ability in downregulating the expression of IGF1R and the activation of PI3K/Akt signaling. Moreover, inhibition of Linc00210 suppressed the growth of TC cells in nude mice. Our findings for the first time uncovered the oncogenic property of Linc00210 in TC.


Assuntos
MicroRNAs/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , RNA Longo não Codificante/metabolismo , Receptor IGF Tipo 1/metabolismo , Neoplasias da Glândula Tireoide/fisiopatologia , Animais , Linhagem Celular , Movimento Celular , Regulação Neoplásica da Expressão Gênica , Inativação Gênica , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Nus , MicroRNAs/genética , Invasividade Neoplásica , Neoplasias Experimentais , Proteínas Proto-Oncogênicas c-akt/genética , RNA Longo não Codificante/genética , Receptor IGF Tipo 1/genética , Glândula Tireoide/metabolismo , Regulação para Cima
9.
Opt Express ; 28(14): 20573-20586, 2020 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-32680114

RESUMO

In this paper, a new method to calibrate a trinocular vision sensor is presented. A planar target with several parallel lines is utilized. The trifocal tensor of three image planes can be calculated out according to line correspondences. Compatible essential matrix between each two cameras can be obtained. Then, rotation matrix and translation matrix can be deduced base on singular value decomposition of their corresponding essential matrix. In our proposed calibration method, image rectification is carried out to remove perspective distortion. As the feature utilized is straight line, precise point to point correspondence is not necessary. Experimental results show that our proposed calibration method can obtain precise results. Moreover, the trifocal tensor can also give a strict constraint for feature matching as descripted in our previous work. Root mean square error of measured distances is 0.029 mm with regards to the view field of about 250×250 mm. As parallel feature exists widely in natural scene, our calibration method also provides a new approach for self-calibration of a trinocular vision sensor.

10.
J Opt Soc Am A Opt Image Sci Vis ; 37(7): 1076-1082, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32609668

RESUMO

A three-dimensional laser scanner has been designed and widely utilized in many fields. The lens plane is tilted according to the Scheimpflug condition and the optical axis is not perpendicular to the charge-coupled device plane. In this case, depth of view can be extended significantly. In this paper, analytical models for a camera with a tilted lens and a laser scanner meeting the Scheimpflug condition are presented. Based on these models, a calibration procedure is detailed. We propose a simple calibration method to determine the intrinsic parameters of a Scheimpflug camera. Meanwhile, two calibration methods for a laser scanner in the Scheimpflug condition are detailed. According to the obtained intrinsic parameters, the laser scanner can be calibrated directly. Moreover, a simple calibration for a three-dimensional laser scanner without the help of a precise positioning system is described. Experimental results show the effectiveness and high measurement accuracy of our calibration methods.

11.
Appl Microbiol Biotechnol ; 103(10): 4153-4165, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30949808

RESUMO

Bacillus spp. are important producers of bioactive natural products with potential applications in medicine and agriculture. Bacillus sp. SCSIO 05476 from a deep-sea sediment exhibits broad-spectrum antimicrobial activities and strong cytotoxic activity. Here, an integrative approach combining genome mining and metabolic profiling has been applied to decipher the chemical origins of this strain's varied and significant biological activities. First, genome mining revealed 19 candidate gene clusters encoding the biosynthesis of diverse secondary metabolites. Then, a series of bacillibactins, fengycins, bacillomycins, surfactins, bacillaenes, macrolactins, and related species were found by LC-DAD-MS. Finally, three new linear bacillibactins, linbacillibactins A-C (1-3), along with 11 known secondary metabolites, bacillibactin (4), normal-C13 Val7 surfactin (5), anteiso-C13 Leu7 surfactin (6), iso-C14 Leu7 surfactin (7), normal-C14 Leu7 surfactin (8), anteiso-C14 Leu7 surfactin (9), macrolactin D (10), normal-C14 bacillomycin D (11), iso-C16 bacillomycin D (12), normal-C17 bacillomycin D (13), and iso-C17 bacillomycin D (14), were obtained and elucidated by bioactivity and structure-guided isolation from the fermentation of strain SCSIO 05746. Among them, new compounds 1-3 show significant siderophore activities comparable to that of bacillibactin (4), compounds 13 and 14 exhibit strong cytotoxic activity. At the same time, the strain classification status was confirmed by genomic analyses, and the complete genome sequence of Bacillus siamensis was presented firstly. This study provides a foundation for understanding the mechanisms driving SCSIO 05746's multiple bioactivities and demonstrates a successful way of discovering bioactive metabolites using a combination of genome mining and metabolic profiling methods.


Assuntos
Bacillus/química , Produtos Biológicos/análise , Genoma Bacteriano , Genômica , Redes e Vias Metabólicas/genética , Metabolômica , Bacillus/genética , Mineração de Dados
12.
Mar Drugs ; 17(2)2019 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-30795576

RESUMO

Aborycin is a ribosomally synthesized member of the type I lasso peptide natural products. In the present study, aborycin was isolated and identified from the deep-sea-derived microbe Streptomyces sp. SCSIO ZS0098. The aborycin biosynthetic gene cluster (abo) was identified on the basis of genome sequence analyses and then heterologously expressed in Streptomyces coelicolor M1152 to effectively produce aborycin. Aborycin generated in this fashion exhibited moderate antibacterial activity against 13 Staphylococcus aureus strains from various sources with minimum inhibitory concentrations MICs = 8.0~128 µg/mL, against Enterococcus faecalis ATCC 29212 with an MIC = 8.0 µg/mL, and against Bacillus thuringiensis with MIC = 2.0 µg/mL. Additionally, aborycin displayed potent antibacterial activity (MIC = 0.5 µg/mL) against the poultry pathogen Enterococcus gallinarum 5F52C. The reported abo cluster clearly has the potential to provide a means of expanding the repertoire of anti-infective type I lasso peptides.


Assuntos
Peptídeos/farmacologia , Streptomyces/genética , Sequência de Aminoácidos , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Bacillus thuringiensis/efeitos dos fármacos , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/farmacologia , Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Produtos Biológicos/farmacologia , Enterococcus/efeitos dos fármacos , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/metabolismo , Família Multigênica , Peptídeos/química , Peptídeos/genética , Peptídeos/isolamento & purificação , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/metabolismo , Streptomyces/metabolismo , Streptomyces coelicolor/efeitos dos fármacos , Streptomyces coelicolor/metabolismo
13.
Molecules ; 23(4)2018 04 19.
Artigo em Inglês | MEDLINE | ID: mdl-29671780

RESUMO

Four metabolites (1⁻4), including a new macrolide, O-demethylated-zeaenol (2), and three known compounds, zeaenol (1), adenosine (3), and ergosta-5,7,22-trien-3b-ol (4) were isolated and purified from Curvularia crepinii QTYC-1, a fungus residing in the gut of Pantala flavescens. The structures of isolated compounds were identified on the basis of extensive spectroscopic analysis and by comparison of the corresponding data with those reported in the literature previously. The new compound 2 showed good phytotoxic activity against Echinochloa crusgalli with an IC50 value of less than 5 µg/mL, which was comparable to that of positive 2,4-dichlorophenoxyacetic acid (2,4-D). Compound 1 exhibited moderate herbicidal activity against E. crusgalli with an IC50 value of 28.8 µg/mL. Furthermore, the new metabolite 2 was found to possess moderate antifungal activity against Valsa mali at the concentration of 100 µg/mL, with the inhibition rate of 50%. These results suggest that the new macrolide 2 and the known compound 1 have potential to be used as biocontrol agents in agriculture.


Assuntos
Antifúngicos/química , Trato Gastrointestinal/microbiologia , Odonatos/microbiologia , Adenosina/química , Animais , Ascomicetos/efeitos dos fármacos , Macrolídeos/química
14.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 35(5): 638-643, 2018 Oct 10.
Artigo em Zh | MEDLINE | ID: mdl-30298485

RESUMO

OBJECTIVE: To detect potential mutations of PHEX gene in four pedigrees affected with hypophosphatemic rickets (HR) and provide prenatal diagnosis for a fetus at 13th gestational week. METHODS: The coding regions and exon/intron boundaries of PHEX, FGF23, DMP1, ENPP1, CLCN5 and SLC34A3 genes of the probands were analyzed by targeted next-generation sequencing (NGS). Suspected mutations were verified by Sanger sequencing among unaffected relatives and 200 unrelated healthy individuals. Deletions were confirmed by multiplex ligation-dependent probe amplification (MLPA) detection of probands, unaffected relatives and 20 unrelated healthy individuals. Prenatal diagnosis for a fetus with high risk was carried out through MLPA analysis. RESULTS: Four PHEX mutations were respectively detected in the pedigrees, which included c.850-3C>G, exon 11 deletion, exon 13 deletion and c.1753G>A (p.G585R). Among these, exon 11 deletion, exon 13 deletion and c.1753G>A (p.G585R) were novel mutations and not found among unaffected relatives and healthy controls. In pedigree 3, the same mutation was not found in the fetus. CONCLUSION: Mutations of the PHEX gene probably underlies the disease among the four pedigrees. NGS combined with Sanger sequencing and/or MLPA detection can ensure accurate diagnosis for this disease.


Assuntos
Raquitismo Hipofosfatêmico/genética , Adulto , Sequência de Bases , Pré-Escolar , Canais de Cloreto/genética , Análise Mutacional de DNA , Proteínas da Matriz Extracelular/genética , Feminino , Fator de Crescimento de Fibroblastos 23 , Fatores de Crescimento de Fibroblastos/genética , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Íntrons , Masculino , Dados de Sequência Molecular , Mutação , Linhagem , Fosfoproteínas/genética , Diester Fosfórico Hidrolases/genética , Gravidez , Pirofosfatases/genética , Raquitismo Hipofosfatêmico/diagnóstico , Proteínas Cotransportadoras de Sódio-Fosfato Tipo IIc/genética
15.
Mol Cell Biochem ; 420(1-2): 95-106, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27449933

RESUMO

Insufficient insulin produced by pancreatic ß-cells in the control of blood sugar is a central feature of the etiology of diabetes. Reports have shown that endoplasmic reticulum (ER) stress is fundamentally involved in ß-cell dysfunction. In this study, we hypothesized that NAD-dependent deacetylase sirtuin-3 (SIRT3), an important regulator of cell metabolism, protects pancreatic ß-cells from ER stress-mediated apoptosis. To validate our hypothesis, a rat diabetic model was established by a high-fat diet (HFD). We found that SIRT3 expression was markedly decreased in NIT1 and INS1 cells incubated with palmitate. Palmitate treatment significantly decreased ß-cell viability and insulin secretion, and promoted malondialdehyde (MDA) formation. However, SIRT3 overexpression in NIT1 and INS1 cells reversed these effects, resulting in higher insulin secretion, decreased ß-cell apoptosis, and downregulation of the expression of ER stress-associated genes. Moreover, SIRT3 overexpression also inhibited calcium influx and the hyperacetylation of glucose-regulated protein of 78 kDa (GRP78). SIRT3 knockdown effectively enhanced the upregulation of phospho-extracellular regulated protein kinases (pERK), inositol-requiring enzyme-1 (IRE1), activating transcription factor 6 (ATF6), and C/EBP homologous protein (CHOP) induced by palmitate, and promoted palmitate-induced ß-cell apoptosis and dysfunction. Taken together, our results suggest that SIRT3 is an integral regulator of ER function and that its depletion might result in the hyperacetylation of critical ER proteins that protect against islet lipotoxicity under conditions of nutrient excess.


Assuntos
Apoptose , Sinalização do Cálcio , Estresse do Retículo Endoplasmático , Regulação Enzimológica da Expressão Gênica , Células Secretoras de Insulina/enzimologia , Sirtuínas/biossíntese , Animais , Células Secretoras de Insulina/patologia , Masculino , Ratos , Ratos Sprague-Dawley
16.
Wei Sheng Wu Xue Bao ; 56(9): 1513-20, 2016 Sep.
Artigo em Zh | MEDLINE | ID: mdl-29738224

RESUMO

Objective: To isolate the fungus with phytotoxic activity from the gut of Pantala flavescens larvae and find the phytotoxic lead compound from the fungal metabolites. Methods: QTYC01 was isolated from the gut of P. flavescens larvae by means of spread plate and identified by 5.8S rRNA sequence analysis and morphologic observation. Phytotoxic activities of the fermentation broth and ethyl acetate extracts against the radical growth of weeds as well as the safety of crude extract to the selected crops were tested by Petri dish bioassay. The herbicidal activity of QTYC01 against Echinochloa crusgalli seedlings was carried out by potted bioassay. Fermentation product was purified by recrystallization and identified by extensive spectroscopic analysis. Results: QTYC01 was identified as Curvularia crepinii. The fermentation broth of QTYC01 significantly inhibited the radical growth of E. crusgalli and Amaranthus retroflexus with the inhibition rate of 95.0% and 90.1%, respectively. The fermented liquid showed significant inhibitory activity to the seedling of E. crusgalli with the victimization rate of 71.1%. Under the concentration of 100 µg/mL, ethyl acetate extracts exhibited significant phytotoxic activities against the radical growth of E. crusgalli and A. retroflexus with inhibitory rates of 56.8% and 71.2%, respectively, and showed good security to the selected common crops with the inhibition rate of lower than 33%. Therewith, a bioactive compound was isolated from the ethyl acetate extract and determined as (5Z)-7-oxozeaenol. The compound showed good phytotoxic activity against A. retroflexus with the IC50 value of 4.8 µg/mL. Conclusion: Strain QTYC01 could be potentially developed as a new microbial herbicide.


Assuntos
Ascomicetos/química , Herbicidas/toxicidade , Odonatos/microbiologia , Amaranthus/efeitos dos fármacos , Amaranthus/crescimento & desenvolvimento , Animais , Ascomicetos/genética , Ascomicetos/isolamento & purificação , Ascomicetos/metabolismo , Echinochloa/efeitos dos fármacos , Echinochloa/crescimento & desenvolvimento , Trato Gastrointestinal/microbiologia , Herbicidas/química , Herbicidas/metabolismo , Larva/microbiologia , Filogenia
17.
Wei Sheng Wu Xue Bao ; 55(3): 292-8, 2015 Mar 04.
Artigo em Zh | MEDLINE | ID: mdl-26065271

RESUMO

OBJECTIVE: To study the inhibitory effect of wasp gut fungus against the radicle growth of Amaranthus retroflexus for the development of herbicides from microorganisms. METHODS: Eleven strains were isolated from wasp gut. Among them the fermentation broth of strain MF06 showed potent herbicidal activity against A. retroflexus. MF06 was identified by morphological observation and molecular biology identification. Fermentation product was isolated and purified by silica column chromatography, TLC and Sephadex LH-20 column chromatography. Metabolite 1 was obtained from fermentation product, and the inhibitory effect of metabolite 1 against the radicle growth of A. retrofexus was studied. The structure was determined by mass spectrum and nuclear magnetic resonance analyses. RESULTS: By the morphological observation and ITS sequence analysis, MF06 was identified as Fusarium oxysporum. The ethyl acetate extract of MF06 had strong activity against A. retrofexus with inhibition rate of more than 68% under the concentration of 100 µg/mL. It is better than other polarities crude extracts. Metabolite 1 was separated from ethyl acetate extract, and it was determined as a mixture of fusaric acid and 9 ,10-dehydrofusaric acid. The mixture is co-crystallizing in a 1:1 molar stoichiometry. It inhibited radical growth of A. retroflexus with IC50 value of (0.51 ± 0.18) µg/mL, comparable to that of 2-( 2,4- dichlorophenoxy) acetic acid (0.30 ± 0.14 µg/mL) used as a positive control. CONCLUSION: Strain MF06 could be potentially developed as a microbial herbicide.


Assuntos
Amaranthus/crescimento & desenvolvimento , Fusarium/química , Fusarium/isolamento & purificação , Vespas/microbiologia , Amaranthus/efeitos dos fármacos , Animais , Ácido Fusárico/metabolismo , Ácido Fusárico/farmacologia , Fusarium/genética , Fusarium/metabolismo , Trato Gastrointestinal/microbiologia , Dados de Sequência Molecular , Filogenia
18.
Front Pharmacol ; 15: 1379821, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39092227

RESUMO

Diabetic kidney disease (DKD) is characterized by complex pathogenesis and poor prognosis; therefore, an exploration of novel etiological factors may be beneficial. Despite glycemic control, the persistence of transient hyperglycemia still induces vascular complications due to metabolic memory. However, its contribution to DKD remains unclear. Using single-cell RNA sequencing data from the Gene Expression Omnibus (GEO) database, we clustered 12 cell types and employed enrichment analysis and a cell‒cell communication network. Fibrosis, a characteristic of DKD, was found to be associated with metabolic memory. To further identify genes related to metabolic memory and fibrosis in DKD, we combined the above datasets from humans with a rat renal fibrosis model and mouse models of metabolic memory. After overlapping, NDRG1, NR4A1, KCNC4 and ZFP36 were selected. Pharmacology analysis and molecular docking revealed that pioglitazone and resveratrol were possible agents affecting these hub genes. Based on the ex vivo results, NDRG1 was selected for further study. Knockdown of NDRG1 reduced TGF-ß expression in human kidney-2 cells (HK-2 cells). Compared to that in patients who had diabetes for more than 10 years but not DKD, NDRG1 expression in blood samples was upregulated in DKD patients. In summary, NDRG1 is a key gene involved in regulating fibrosis in DKD from a metabolic memory perspective. Bioinformatics analysis combined with experimental validation provided reliable evidence for identifying metabolic memory in DKD patients.

19.
Acta Diabetol ; 61(8): 963-974, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38583120

RESUMO

BACKGROUND: Metabolic unhealth (MUH) is closely associated with cardiovascular disease (CVD). Life's Essential 8 (LE8), a recently updated cardiovascular health (CVH) assessment, has some overlapping indicators with MUH but is more comprehensive and complicated than MUH. Given the close relationship between them, it is important to compare these two measurements. METHODS: This population-based cross-sectional survey included 20- to 80-year-old individuals from 7 National Health and Nutrition Examination Survey (NHANES) cycles between 2005 and 2018. Based on the parameters provided by the American Heart Association, the LE8 score (which ranges from 0 to 100) was used to classify CVH into three categories: low (0-49), moderate (50-79), and high (80-100). The MUH status was evaluated by blood glucose, blood pressure, and blood lipids. The associations were assessed by multivariable regression analysis, subgroup analysis, restricted cubic spline models, and sensitivity analysis. RESULTS: A total of 22,582 participants were enrolled (median of age was 45 years old), among them, 11,127 were female (weighted percentage, 49%) and 16,595 were classified as MUH (weighted percentage, 73.5%). The weighted median LE8 scores of metabolic health (MH) and MUH individuals are 73.75 and 59.38, respectively. Higher LE8 scores were linked to lower risks of MUH (odds ratio [OR] for every 10 scores increase, 0.53; 95% CI 0.51-0.55), and a nonlinear dose-response relationship was seen after the adjustment of potential confounders. This negative correlation between LE8 scores, and MUH was strengthened among elderly population. CONCLUSIONS: Higher LE8 and its subscales scores were inversely and nonlinearly linked with the lower presence of MUH. MUH is consistent with LE8 scores, which can be considered as an alternative indicator when it is difficult to collect the information of health behaviors.


Assuntos
Doenças Cardiovasculares , Inquéritos Nutricionais , Humanos , Feminino , Masculino , Pessoa de Meia-Idade , Estudos Transversais , Estados Unidos/epidemiologia , Idoso , Adulto , Doenças Cardiovasculares/epidemiologia , Idoso de 80 Anos ou mais , Adulto Jovem , Glicemia/metabolismo , Glicemia/análise , Pressão Sanguínea , Lipídeos/sangue
20.
Insect Sci ; 2024 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-38494587

RESUMO

Using synthetic microbial communities to promote host growth is an effective approach. However, the construction of such communities lacks theoretical guidance. Kin discrimination is an effective means by which strains can recognize themselves from non-self, and construct competitive microbial communities to produce more secondary metabolites. However, the construction of cooperative communities benefits from the widespread use of beneficial microorganisms. We used kin discrimination to construct synthetic communities (SCs) comprising 13 Bacillus subtilis strains from the surface and gut of black soldier fly (BSF) larvae. We assessed larval growth promotion in a pigeon manure system and found that the synthetic community comprising 4 strains (SC 4) had the most profound effect. Genomic analyses of these 4 strains revealed that their complementary functional genes underpinned the robust functionality of the cooperative synthetic community, highlighting the importance of strain diversity. After analyzing the bacterial composition of BSF larvae and the pigeon manure substrate, we observed that SC 4 altered the bacterial abundance in both the larval gut and pigeon manure. This also influenced microbial metabolic functions and co-occurrence network complexity. Kin discrimination facilitates the rapid construction of synthetic communities. The positive effects of SC 4 on larval weight gain resulted from the functional redundancy and complementarity among the strains. Furthermore, SC 4 may enhance larval growth by inducing shifts in the bacterial composition of the larval gut and pigeon manure. This elucidated how the SC promoted larval growth by regulating bacterial composition and provided theoretical guidance for the construction of SCs.

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