A chromosome-scale assembly of Brassica carinata (BBCC) accession HC20 containing resistance to multiple pathogens and an early generation assessment of introgressions into B. juncea (AABB).

Brassica carinata (BBCC) commonly referred to as Ethiopian mustard is a natural allotetraploid containing the genomes of Brassica nigra (BB) and Brassica oleracea (CC). It is an oilseed crop endemic to the northeastern regions of Africa. Although it is under limited cultivation, B. carinata is valuable as it is resistant/highly tolerant to most of the pathogens affecting widely cultivated Brassica species of the U's triangle. We report a chromosome-scale genome assembly of B. carinata accession HC20 using long-read Oxford Nanopore sequencing and Bionano optical maps. The assembly has a scaffold N50 of ~39.8 Mb and covers ~1.11 Gb of the genome. We compared the long-read genome assemblies of the U's triangle species and found extensive gene collinearity between the diploids and allopolyploids with no evidence of major gene losses. Therefore, B. juncea (AABB), B. napus (AACC), and B. carinata can be regarded as strict allopolyploids. We cataloged the nucleotide-binding and leucine-rich repeat immune receptor (NLR) repertoire of B. carinata and, identified 465 NLRs, and compared these with the NLRs in the other Brassica species. We investigated the extent and nature of early-generation genomic interactions between the constituent genomes of B. carinata and B. juncea in interspecific crosses between the two species. Besides the expected recombination between the constituent B genomes, extensive homoeologous exchanges were observed between the A and C genomes. Interspecific crosses, therefore, can be used for transferring disease resistance from B. carinata to B. juncea and broadening the genetic base of the two allotetraploid species.

Enhanced exopolysaccharide production by multi metal tolerant Klebsiella variicolaSMHMZ46 isolated from mines area and application in metal bioremediation.

The current study aimed to enhance exopolysaccharide production by Klebsiella variicolaSMHMZ46 isolated from the Zawar mines area in Udaipur, Rajasthan, India, by optimizing the medium with OFAT and a central composite design. The trial including sucrose (9.5%), casein hydrolysate (3%), and NaCl (0.5%) yielded the maximum EPS production as indicated by applying the CCD-RSM biostatistical program. The composition of exopolysaccharides produced by Klebsiella variicolaSMHMZ46 culture was characterized. Growth under Pb(II), Cd(II), and Ni(II) metal amended conditions induced EPS production relative to control. TLC was used for identifying the sugar residues of EPS, in addition to determination of both total carbohydrate and protein contents. According to FT-IR analysis, EPS can interact with metal ions via their functional chemical groups, thereby supporting their bioremediation potential. The metal removal efficiency of bacteria and their produced EPS in broth individually spiked with Pb(II), Ni(II), and Cd(II) was 99.18%, 97.60%, and 98.20%, respectively, and powdered EPS from contaminated water was 85.76%, 72.40%, and 71.53%, respectively. According to FEG-SEM observations, the surface morphology of EPS becomes rough, demonstrating sharp bumps after metal binding. A FEG-SEM analysis of the structure of EPS was performed; the surface structure of EPS (with metal) was more rigid than that of control EPS (without metal). The interaction between the EPS system and Pb(II) ions was investigated using FEG-SEM coupled with energy dispersive X-ray spectra, and a strong peak of C, O, and Pb elements was observed, indicating successful Pb adsorption. These findings suggest that EPS from Klebsiella variicolaSMHMZ46 has a good metal adsorbing nature and could be a promising biosorbent for metal bioremediation of contaminated water.

Treatment of pharmaceutical wastewater through activated sludge process-a critical review.

The occurrence of pharmaceutical compounds in water is a rising issue in the environment. These drugs in the waste may be toxic to aquatic organisms and humans as they disrupt the endocrine system, cause genotoxicity, etc. Several techniques were used for the treatment of pharmaceutical wastewater, such as physical, chemical, physiochemical, and biological processes like adsorption, chemical coagulation, and activated sludge processes, but these techniques possess several merits and demerits, such as higher installation and operation costs. This technique is used to remove color and turbidity; reduce biochemical oxygen demand (BOD), chemical oxygen demand (COD), and total suspended solids (TSS) to permissible limits for reuse of effluent; and prevent diseases caused by pharmaceutical wastewater. This review focuses on the treatment of pharmaceutical wastewater containing drugs like antibiotics, depressants, and hormones, with the activated sludge process having several advantages like good quality effluent and low installation costs.

Synergistic optimization of thermoelectric performance in earth-abundant Cu2ZnSnS4 by inclusion of graphene nanosheets.

Earth-abundant quaternary chalcogenide semiconductors with complex structures, such as copper zinc tin sulphide (Cu2ZnSnS4; CZTS), have the potential to become economic and non-toxic thermoelectric materials. However, the inferior power factor of CZTS, due to its insignificant electrical conductivity, negates the advantage of inherent small thermal conductivity. In the present report, the thermoelectric properties of CZTS composites integrated with graphene nanosheets (GNs) CZTS/x (x = 0.25, 0.5, 0.75 or 1 wt% GNs) were synergistically optimized. The inclusion of GNs (⩽0.75 wt%) simultaneously enhanced the carrier transport (electrical conductivity σ) by providing conductive pathways as well as suppressed lattice thermal conductivity (κ L) due to the enhanced grain barriers in addition to interface scattering. This synergistic optimization enhanced the figure of merit, ZT, of CZTS/GN nanocomposites to its highest value (∼0.5) at 623 K for the addition of 0.75 wt% GNs, which is nearly a seven-fold enhancement over the pristine sample. The novel strategy of fabricating CZTS/GN nanocomposites by utilizing GNs is an alternative way to obtain the highest thermoelectric performance (ZT ∼ 0.5) in CZTS, and can be extended to other environmentally friendly quaternary chalcogenides.

Prevalence of Integrase Strand Inhibitor Resistance (INSTI)in New Orleans, Louisiana, 2012-2013.

Integrase strand inhibitors (INSTI) are the newest class of antiretroviral (ARV) therapies available to patients. There is little surveillance data on INSTI resistance, particularly in the South. A retrospective review on all patients who underwent PhenoSense Integrase testing between January 1, 2012 and June 30, 2013 was performed to better identify who should be screened for integrase strand transfer inhibitor (INSTI) resistance at the Interim Louisiana State University Hospital (ILH). Fifty-three patients underwent PhenoSense testing for elvitegravir/raltegravir resistance during this time period. Resistance was significantly associated with INSTI experience but not with other characteristics including sex, age, race, CD4 cell count, or concurrent reverse transcriptase inhibitor or protease inhibitor mutations. The frequency of reduced elvitegravir/ raltegravir susceptibility among INSTI experienced patients with virologic failure at ILH was 14/41 (34%). Future surveillance monitoring will be useful for determining if baseline INSTI resistance testing needs to be performed for INSTI naïve patients in the future at ILH.

Unraveling Factors Shaping the Acceptance and Non-acceptance of Non-scalpel Vasectomy in Rural Central India: A Cross-Sectional Study.

Background India, with a population exceeding 1.43 billion, faces significant demographic challenges, necessitating effective family planning measures. Non-scalpel vasectomy (NSV) is a less complex and cost-effective male contraceptive, yet its acceptance remains low, especially compared to female sterilization. Understanding the determinants of NSV acceptance is crucial for informed decisions on family planning methods. Methods This cross-sectional study investigated NSV acceptance among males who underwent non-scalpel vasectomy (acceptors of NSV) and spouses of women who underwent tubectomy (non-acceptors of NSV). The study was conducted among 116 NSV acceptors and 116 non-acceptors from rural Central India. Data were collected over six months, employing a pre-designed questionnaire covering socio-demographic details, reasons for acceptance/non-acceptance, and information sources for the same. Statistical analysis was done utilizing Epi Info 7.2.6 (Centers for Disease Control and Prevention [CDC], Atlanta, GA), employing descriptive statistics and tests of association. Results Significant associations were found between NSV acceptance (p<0.05) and the age and education of study subjects, age of wife, duration since marriage, and total number of children. Incentives also played a significant role (p=0.014). Opposition to NSV, mainly from wives, was a key factor for non-acceptance (38%). Reasons for acceptance included a previous cesarean section (40.52%), the simplicity of the NSV procedure (26.72%), and the wife's illness (23.28%). Non-acceptance reasons comprised family/friend opposition (38%), lack of awareness/ignorance (25.00%), and fear of surgery (23.28%). Study subjects perceived community reluctance to NSV as mainly due to misbeliefs (30.17%), fear of surgery (27.58%), and illiteracy (26.29%). Conclusion The study highlights socio-demographic factors influencing NSV acceptance and identifies key reasons for acceptance/non-acceptance. Community-based interventions, increased advertisement, and health provider counseling were suggested for enhancing acceptance. Despite challenges, participant satisfaction with NSV was high. These findings contribute to understanding the complex dynamics surrounding NSV acceptance in rural Central India, informing future family planning strategies.

Effect of oral vitamin C administration along with iron supplementation for treating anaemia among adolescent girls - Protocol for systematic review and meta-analysis.

Background: Anemia among adolescent girls is a worldwide public health problem. Although anemia has several causes, the most common and significant is iron deficiency. Treatment of anemia with iron alone may result in reduced compliance on account of adverse effects. Various studies have revealed that use of vitamin C in addition to iron improves its absorption and reduces the ill effects of the therapy. However, a concrete evidence of adding vitamin C to iron for treating iron deficiency anemia is lacking. Aims: Protocol for current systematic review was prepared and registered with the aim to generate evidence on role of vitamin C in addition to iron for treatment of anemia. Materials and Methods: Present protocol has been prepared based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and registered with International Prospective Register of Systematic Reviews. Data extraction will be done by the reviewers independently. Cochrane risk of bias assessment tool will be used for risk of bias assessment. Results: For primary outcome and each of the other outcomes pairwise random effects meta-analysis and network meta-analysis will be performed. Sensitivity analysis will be performed in case of any heterogeneity is detected to find out the difference of the effect estimation between subsets. Conclusions: The evidence for role of vitamin C as an adjunct in iron and folic acid for treatment of anemia in adolescent girls will be generated once the systematic review and meta-analysis is completed.

Biofilm-forming plant growth-promoting rhizobacterial consortia isolated from mines and dumpsites assist green remediation of toxic metal (Ni and Pb) using Brassica juncea.

To study how biofilm-forming rhizobacteria isolated from mines and dumpsites improved the phytoremediation efficacy of B. juncea in metal-contaminated soil. Out of 91 isolates, six were chosen for research based on their tolerance to metals, and their efficient PGPR properties, and subjected to the design of a consortium. A compatibility study revealed no antagonistic interaction between rhizobacterial-consortiums. The results of the biofilm formation and FEG-SEM studies revealed that a consortium-BC8 formed a strong biofilm on the root surface of B. juncea seedlings. Based on results obtained with the phytoextraction efficiency of B. juncea in consortium-BC8 (SMHMZ46 and SMHMP23), they were identified as Klebsiella variicola and Pseudomonas otitidis, respectively, and submitted to NCBI GenBank with accession numbers MZ145092 and OK560623. This rhizobacteria is the first to be reported as assisting Ni and Pb phytoremediation by employing B. juncea. Soil inoculation with consortium-BC8 increased the amount of soluble Ni and Pb by 13.25-fold and 10.69-fold, respectively, when compared to the control. These consortiums-BC8 significantly increased vegetative growth and metal accumulation in root and shoot with a translocation-factor of 1.58 for Ni and soil to root with a bioconcentration-factor of 1.3 for Pb in B. juncea grown in individual soil contamination with 96.05 mg/kg NiCl2 and 89.63 mg/kg Pb(NO3)2, which are significantly higher than other consortium treatments and the non-inoculated control. B. juncea amendments with a biofilm-forming consortium-BC8 having TF, BCF, and BAC > 1 for Ni, whereas BCF > 1, TF, and BAC < 1 for Pb, are appropriate for green remediation of Ni and phytostabilization of Pb.

Epidemiology of Primary Headache and Its Associated Psychosocial Factors Amongst Undergraduate Medical Students: A Cross-Sectional Study From the Vidarbha Region.

Introduction The present study was carried out to investigate the epidemiology of primary headaches amongst undergraduate medical students by determining the prevalence of primary headaches and their associated psychosocial factors. Methods A cross­sectional study was conducted at a medical college in the Vidarbha region of India from January 2023 to February 2023 amongst 471 medical students. Diagnosis of tension-type headache (TTH) was made according to the International Classification of Headache Disorders (ICHD-3) criteria. Data were collected by interview technique using a pre-designed and pre-tested questionnaire. that consisted of socio-demographic variables and psychosocial factors. Statistical analysis was done using SPSS 24.0 (IBM Corp., Armonk, NY, USA).  Results Prevalence of headache was 80% and was higher in females (87%) than in males (71%). TTH is the most common type with a prevalence of 76% in females followed by 64% in males. Psychosocial factors associated with presence of headache in study subjects were disappointment in relation to academic performance (OR 3.85, CI 1.68-2.71), poor socio-economic status (OR 2.69, CI 1.58-4.57), work overload (OR 0.41, CI 0.24-0.68), irritability (OR 0.33, CI 0.19-0.57) and frequent conflicts (OR 1.45, CI 0.78-2.70). Stress (OR 0.27, CI 0.11-0.71) and anxiety (OR 3.45, CI 1.31-9.08) were associated with headaches only in females and depression (OR 0.50, CI 0.25-1.01) was found to be associated with headaches only in males. Conclusions Psychosocial factors from the personal sphere like stress, overwork, and anxiety were highly prevalent amongst students and these factors need to be addressed meticulously in order to mitigate the problem of primary headache disorders amongst medical undergraduates.

Effect of Dietary Modification and Physical Activity on Obese Young Adults Going to Gym for Weight Loss in Central India: A Before and After Study.

BACKGROUND: An adequate diet is a prerequisite for appropriate growth and development so as to remain active. Balanced nutrition coupled with physical activity forms a healthy lifestyle which eventually leads to multiple health benefits such as positive mental health and a lower risk of noncommunicable diseases like diabetes, hypertension, etc. It has become an upcoming trend for young adults to join a gym. In order to maintain weight members, go to the gym because they consider it as a positive opportunity to boost self-esteem and to make appropriate health decisions to feel better. There is a need to highlight with the help of research studies that lifestyle modification in the form of diet and physical activity on a regular basis can help in controlling obesity. METHODS: A before and after the study was carried out in Nagpur city located in Central India for a period of 8 months (April 1, 2022 to November 30, 2022). The study subjects were obese young adults going to the gym in Nagpur city. Diet and physical activity interventions were given for a duration of three months to the study subjects. The study instrument was a predesigned and pretested questionnaire. RESULTS: In the present study, total 110 study subjects responded by completely filling out the questionnaire The total calorie and protein intake of the study subjects before and after giving intervention for three months was found to be highly significant (p-value < 0.0001). The change in mean anthropometric parameters of the study subjects before and after giving intervention for three months was found to be highly significant (p-value < 0.0001). CONCLUSIONS: Dietary modification combined with physical activity for an average of 75 minutes is the most effective short-term intervention for weight loss. The present study concludes that lifestyle modifications can reverse the trend of obesity. It is reversible, and obese individuals can normalize their BMI with appropriate interventions as was performed with the present obese study subjects.

Age-Old Dilemma of Pregnancy During Residency: A Cross-Sectional Study From Central India on Perceptions and Experiences of Female Medicos.

Introduction The integration of family and career poses a significant problem for women in the medical profession. Balancing residency program demands with burgeoning family issues has always been a dilemma for female medicos. Lack of support and sometimes hostility from life partners, program administrators, teachers, and other residents have been reported. The present study is an attempt to assess perceptions and experiences of female medicos regarding pregnancy during residency. Methods The present descriptive cross-sectional study was carried out in a government medical college and hospital, which is a tertiary care center as well as a public sector teaching and training institute located in central India. Data were collected by interview technique using a predesigned and pretested questionnaire. Data were analyzed using the statistical software Epi Info version 7.2.5 (CDC, Atlanta, Georgia). Mean and standard deviations were calculated for continuous variables and the chi-square test was applied for categorical variables. Results Of the 612 study subjects, 409 (66.8%) belonged to the clinical disciplines and 203 (33.2%) were from nonclinical and paraclinical disciplines. A total of 66 (32.5%) subjects from the paraclinical and nonclinical sides had experienced pregnancy during residency, whereas only 54 (13.2%) from the clinical side were pregnant during residency. Positive influences for pregnancy during residency were concerns about age and fertility, pressure from in-laws and parents, desire for family and pregnancy, etc., all with a mean score of 3.5 and above on a five-point Likert scale. Tight schedules, availability of childcare arrangements, support from faculty and other residents, etc. were relatively negative influences with a mean score of less than 3.5. Around 66% of those from nonclinical and paraclinical groups had conceived before 26 years of their age, whereas only 30% of residents from clinical departments had experienced pregnancy before that age. Thus, the age at conception was relatively lower in residents from nonclinical and paraclinical disciplines as compared to their counterparts from clinical disciplines, and this difference was found to be statistically significant (p < 0.001). The complications during pregnancy were more in clinical residents than in those from the nonclinical and paraclinical side. Conclusions This study concludes that concerns about age and fertility, pressure from in-laws and parents, desire for family and pregnancy, and enjoying children are relatively positive influences on the occurrence of pregnancy, whereas tight schedule, availability of childcare arrangements, support from faculty and other residents, and timing professionally are relatively negative influences.

An Observational Study of Indian Medical Students: Are We Truly Aware of Monkeypox?

Introduction Monkeypox is a zoonotic disease caused by an enveloped double-stranded DNA virus from the Poxviridae family. As the future front-liners of healthcare, it is crucial to equip medical students with adequate knowledge of diseases like monkeypox that pose a potential pandemic threat. Aim This study was planned to evaluate the level of awareness of monkeypox among Indian medical students. Methodology This was a cross-sectional, questionnaire-based research conducted using a web-based platform. The study population consisted of medical (MBBS) students from India studying in their first, second, third, final, and internship years. Data collection was done using a self-designed, semi-structured questionnaire. Results Out of the 511 students who filled out the form, 280 (54.79%) were males and 230 (45.01%) were females. Of the study respondents, 459 (89.82%) had heard about monkeypox. The internet (60.5%) and social media (55.3%) were the most common sources of information about monkeypox. The study participants were found to be fairly aware of the different modes of monkeypox transmission. A significant majority (about three-fourths) of internship-year students demonstrated knowledge about preventive measures for monkeypox, with only a minimal 5% reporting a lack of awareness. In contrast, a substantial percentage of first-year students (38%) and second-year students (37.6%) admitted to being unaware of prevention methods, with a statistically significant difference (p<0.001). Conclusion The overall knowledge levels were generally satisfactory, with respondents demonstrating awareness of different modes of monkeypox transmission. However, a concerning proportion of first-year (38%) and second-year (37.6%) students reported being unaware of prevention methods.

Smartphone Usage Patterns Among Postgraduate Medical Students: A Central India Perspective on Adaptive Learning in Medicine.

Introduction Technological developments are drawn on a path of continuous inventions. Smartphones have been used in educational activities to access course content, acquire information related to students' performance, and encourage discussion and sharing between students and teachers. Students as learners are the drivers of using new technology for their learning needs, and this is always evolving. Aim The aim of the study is to assess the usage of smartphones for academic learning among postgraduate medical students in a teaching institute in Central India. Materials and methods This cross-sectional, questionnaire-based study was conducted on 130 postgraduate medical students for a period of four months from 1 January 2022 to 30 April 2022. Results The mean age of the study subjects was 28.34 ± 3.22 years with the range being 23 to 44 years. Smartphones had a significant impact on the academic learning of postgraduate medical students. The major impacts of smartphones on academic learning were in the form of improved learning skills (60.91%), timely completion of assignments (40%), increased participation in discussions (34.55%), enhanced academic performance (33.64%), and development of critical and innovative thinking (29.09%). Conclusion A significant proportion of medical postgraduates are utilizing smartphones and social media for academic purposes. Although this new technology offers the potential to enhance learning and patient care, it has some inherent problems associated with its use. However, it may go a long way in redefining how we manage information in medicine.

Prevalence, Pattern, and Reasons for Self-Medication: A Community-Based Cross-Sectional Study From Central India.

Introduction Self-medication is an important public health problem, with varied prevalence across the world. The high prevalence of self-medication in India is one of the important factors contributing to the development of antimicrobial resistance. Self-medication without medical guidance can lead to inappropriate, incorrect, or undue therapy, missed diagnosis, delays in appropriate treatment, pathogen resistance, and increased morbidity. The growing trend of self-medication can be attributed to various factors like the urge for self-care, sympathy toward sick family members, inaccessible health services and nonavailability of drugs, time and financial constraints, ignorance, misbeliefs, extensive advertisement and availability of drugs in places other than drug shops. Methodology The present community-based descriptive cross-sectional study was conducted in an urban field practice area of a tertiary health care center (UHTC) in Central India. Individuals above 18 years of age and present at home at the time of the house-to-house survey comprised the study participants. A total of 400 participants were enrolled in the study. Data were collected using a predesigned and pretested questionnaire by the face-to-face interview technique. Results The prevalence of self-medication in the area was 60 % (240). The most widely used drugs for self-medication were analgesics (159; 66.25%) and antipyretics (142; 59.16%). Common ailments for which self-medication was used frequently were fever, body aches, common cold, and cough. It was observed that female participants were twice more likely to self-medicate as compared to male participants (Odds Ratio (OR): 2.04; Prevalence (p) = 0.014, Confidence Interval (CI) 95% = 1.15-3.62). Additionally, those having education above the high school level had more chances of self-medicating than those educated less than high school (OR: 1.25; p≤0.014, CI 95%=1.05-1.50). The commonest reasons for resorting to self-medication as per the findings of the present study are that it saves time and the condition was not serious enough to warrant a physician's consultation.

Risk factors for SARS-CoV-2 infection among health workers in India: a case control study.

Background: COVID-19 was declared as a Public Health Emergency of International Concern on 30th January 2020. Compared to the general population, healthcare workers and their families have been identified to be at a higher risk of getting infected with COVID-19. Therefore, it is crucial to understand the risk factors responsible for the transmission of SARS-CoV-2 infection among health workers in different hospital settings and to describe the range of clinical presentations of SARS-CoV-2 infection among them. Methodology: A nested case-control study was conducted among healthcare workers who were involved in the care of COVID-19 cases for assessing the risk factors associated with it. To get a holistic perspective, the study was conducted in 19 different hospitals from across 7 states (Kerala, Tamil Nadu, Andhra Pradesh, Karnataka, Maharashtra, Gujarat, and Rajasthan) of India covering the major government and private hospitals that were actively involved in COVID-19 patient care. The study participants who were not vaccinated were enrolled using the incidence density sampling technique from December 2020 to December 2021. Results: A total of 973 health workers consisting of 345 cases and 628 controls were recruited for the study. The mean age of the participants was observed to be 31.17 ± 8.5 years, with 56.3% of them being females. On multivariate analysis, the factors that were found to be significantly associated with SARS-CoV-2 were age of more than 31 years (adjusted odds ratio [aOR] 1.407 [95% CI 1.53-1.880]; p = 0.021), male gender (aOR 1.342 [95% CI 1.019-1.768]; p = 0.036), practical mode of IPC training on personal protective equipment (aOR 1. 1.935 [95% CI 1.148-3.260]; p = 0.013), direct exposure to COVID-19 patient (aOR 1.413 [95% CI 1.006-1.985]; p = 0.046), presence of diabetes mellitus (aOR 2.895 [95% CI 1.079-7.770]; p = 0.035) and those received prophylactic treatment for COVID-19 in the last 14 days (aOR 1.866 [95% CI 0.201-2.901]; p = 0.006). Conclusion: The study was able to highlight the need for having a separate hospital infection control department that implements IPC programs regularly. The study also emphasizes the need for developing policies that address the occupational hazards faced by health workers.

Identification of follistatin-like 1 by expression cloning as an activator of the growth differentiation factor 15 gene and a prognostic biomarker in acute coronary syndrome.

BACKGROUND: Growth differentiation factor 15 (GDF15) is a stress-responsive cytokine and biomarker that is produced after myocardial infarction and that is related to prognosis in acute coronary syndrome (ACS). We hypothesized that secreted proteins that activate GDF15 production may represent new ACS biomarkers. METHODS: We expressed clones from an infarcted mouse heart cDNA library in COS1 cells and assayed for activation of a luciferase reporter gene controlled by a 642-bp fragment of the mouse growth differentiation factor 15 (GDF15) gene promoter. We measured the circulating concentrations of follistatin-like 1 (FSTL1) and GDF15 in 1369 patients with ACS. RESULTS: One cDNA clone that activated the GDF15 promoter-luciferase reporter encoded the secreted protein FSTL1. Treatment with FSTL1 activated GDF15 production in cultured cardiomyocytes. Transgenic production of FSTL1 stimulated GDF15 production in the murine heart, whereas cardiomyocyte-selective deletion of FSTL1 decreased production of GDF15 in cardiomyocytes, indicating that FSTL1 is sufficient and required for GDF15 production. In ACS, FSTL1 emerged as the strongest independent correlate of GDF15 (partial R(2) = 0.26). A total of 106 patients died of a cardiovascular cause during a median follow-up of 252 days. Patients with an FSTL1 concentration in the top quartile had a 3.7-fold higher risk of cardiovascular death compared with patients in the first 3 quartiles (P < 0.001). FSTL1 remained associated with cardiovascular death after adjustment for clinical, angiographic, and biochemical variables. CONCLUSIONS: Our study is the first to use expression cloning for biomarker discovery upstream of a gene of interest and to identify FSTL1 as an independent prognostic biomarker in ACS.

Status of Respectful Maternity Care Among Women Availing Delivery Services at a Tertiary Care Center in Central India: A Cross-Sectional Study.

Introduction There are numerous reports of disrespectful, abusive, or neglectful treatment during childbirth from health facilities worldwide. Although India has substantially increased the number of hospital deliveries and reduced the maternal mortality ratio, the quality of intrapartum and immediate postpartum care for delivering mothers has not been given much importance. Therefore, assessing mistreatment and quality of care during childbirth is vital for promoting respectful maternity care.  Methods A descriptive hospital-based cross-sectional study was carried out in a tertiary care center in central India. A convenience sampling method was used, and a total of 150 consecutive consenting women aged 18-49 years who delivered in the study setting were included. Data was collected using a predesigned and pretested questionnaire based on seven major categories per the Respectful Maternity Care (RMC) Charter. Results All the 150 women in the present study, i.e., 100%, experienced at least one form of disrespect during their labor, childbirth, or postnatal period at the hospital. The mean scores for domains of non-confidential care (0.59), non-consented care (0.95), abandonment or denial of care (1.21), and physical abuse (1.26) are low. Conclusion The findings of the present study shed important light on the current state of respectful maternity care in the study area. Though mothers are noticing and reporting positive changes in maternity care practices, respectful maternity care still has a long way to go.

Awareness and Satisfaction About COVAXIN Vaccination Services at an Immunization Clinic in Nagpur: A Cross-Sectional Study.

Introduction Initially, coronavirus disease 2019 (COVID-19) vaccination was started in India for the elderly above 60 years of age. Adults with any comorbidity have been gradually included in the vaccination drive. It is empirical to gain insight into the satisfaction of these beneficiaries with the vaccination as it may act as an influencing factor for receiving the vaccine. Materials and methods This was a descriptive cross-sectional study carried out at the COVID-19 vaccination clinic of the Government Medical College and Hospital, Nagpur, among individuals above 60 years of age and those from 45 to 60 years of age with comorbidity. The survey tool was a predesigned structured questionnaire that had close-ended questions on various aspects of awareness about the COVID-19 vaccines and their satisfaction with the immunization center. Interviews were conducted by two interviewers on each day. Data were analyzed using open software Epi Info (CDC, Atlanta, Georgia). The chi-square test was applied as a test of significance. Results A total of 290 subjects participated in the study. The majority had correct knowledge about COVID-19 vaccination and appropriate COVID-19 behavior after vaccination. Fever and body ache were known to most of the subjects as adverse effects following immunization. Social media was the most common source of knowledge. The majority of the subjects were satisfied with the services provided at the vaccination center, but there was no difference as per age, gender, or residential status of the subjects. Conclusion Despite mixed rumors about the COVID-19 vaccine, the majority of the study subjects were well satisfied with the vaccination. They were apparently having fair awareness about the vaccine.

Rapid determination and optimisation of berberine from Himalayan Berberis lycium by soxhlet apparatus using CCD-RSM and its quality control as a potential candidate for COVID-19.

SARS-CoV-2 (or COVID-19) has become a global risk and scientists are attempting to investigate antiviral vaccine. Berberis are important plants due to the presence of bioactive phytochemicals, especially berberine from the protoberberine group of benzylisoquinoline and recent studies have shown its potential in treating COVID-19. B. lycium Royle growing in subtropical regions of Asia had wide applications in Indian system of medicine. Rapid determination and novel optimisation method for berberine extraction has been developed by Soxhlet extraction utilising central composite design-response surface methodology (CCD-RSM). Berberine was detected by high-performance liquid chromatography (HPLC), and the highest yield (13.39%) was obtained by maintaining optimal extraction conditions i.e., extraction time (7.28 hrs), ethyl alcohol (52.21%) and solvent to sample ratio (21.78 v/w). Investigation of two geographic regions (Ramnagar and Srinagar) showed high berberine content in lower altitude. This novel optimisation technique has placed berberine as a potential candidate for developing pharmaceutical products for human health care.