RESUMO
DNA viruses can hijack and manipulate the host chromatin state to facilitate their infection. Multiple lines of evidences reveal that DNA virus infection results in the host chromatin relocation, yet there is little known about the effects of viral infection on the architecture of host chromatin. Here, a combination of epigenomic, transcriptomic and biochemical assays were conducted to investigate the temporal dynamics of chromatin accessibility in response to Bombyx mori nucleopolyhedrovirus (BmNPV) infection. The high-quality ATAC-seq data indicated that progressive chromatin remodeling took place following BmNPV infection. Viral infection resulted in a more open chromatin architecture, along with the marginalization of host genome and nucleosome disassembly. Moreover, our results revealed that chromatin accessibility in uninfected cells was regulated by euchromatic modifications, whereas the viral-induced highly accessible chromatin regions were originally associated with facultative heterochromatic modification. Overall, our findings illustrate for the first time the organization and accessibility of host chromatin in BmNPV-infected cells, which lay the foundation for future studies on epigenomic regulation mediated by DNA viruses.
Assuntos
Baculoviridae/fisiologia , Bombyx , Eucromatina , Genoma de Inseto , Interações Hospedeiro-Patógeno , Animais , Bombyx/genética , Bombyx/metabolismo , Bombyx/virologia , Linhagem Celular , Eucromatina/genética , Eucromatina/metabolismo , Eucromatina/virologiaRESUMO
ORF40 (bm40) of the Bombyx mori nucleopolyhedrovirus (BmNPV) encodes a homologue of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) AC51 and is a highly conserved gene in sequenced alphabaculoviruses. To investigate the role of bm40 in the baculovirus infection cycle, a bm40 knockout BmNPV bacmid was constructed via homologous recombination in Escherichia coli. Western blotting analysis revealed that bm40 is a late gene during virus infection. Compared with wild-type and repair viruses, the knockout virus exhibited a single-cell infection phenotype. Titration assays confirmed that no infectious budded viruses (BVs) were produced due to the bm40 deletion, while there was no effect on viral DNA replication. Electron microscopy revealed that Bm40 is required for nucleocapsid egress from the nucleus to the cytoplasm, nucleocapsid envelopment to form occlusion-derived viruses (ODVs) and subsequent embedding of ODVs into polyhedra. Confocal microscopy showed that Bm40 was predominantly localized in the nuclei from 48 h post-infection and subsequently condensed on the nuclear membrane and polyhedra at the late phase of infection. Taken together, these results demonstrate that Bm40 plays an essential role in BV production and ODV envelopment in the BmNPV infection cycle.
Assuntos
Nucleopoliedrovírus/genética , Fases de Leitura Aberta/genética , Liberação de Vírus , Replicação Viral , Animais , Bombyx/citologia , Bombyx/virologia , Linhagem Celular , Núcleo Celular/virologia , Citoplasma/virologia , Técnicas de Inativação de Genes , Nucleocapsídeo/metabolismo , Nucleopoliedrovírus/fisiologia , FilogeniaRESUMO
Bombyx mori nucleopolyhedrovirus (BmNPV) orf133 (bm133) and orf134 (bm134), the orthologues of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac4 and ac5, are two adjacent genes with opposite transcriptional orientations and are highly conserved in all sequenced group I nucleopolyhedroviruses (NPVs). A double bm133-bm134 knockout bacmid was generated to enable the functional study of each gene independently or together. Compared with wild-type and double-repair viruses, deletion of both bm133 and bm134 did not affect budded virus (BV) production or viral DNA replication in transfected BmN cells. Electron microscopy revealed that the double knockout did not affect nucleocapsid assembly, virus-induced intranuclear microvesicle formation or occlusion-derived virus (ODV) production, but the number of virions embedded in the polyhedra decreased significantly. Further investigations showed that disruption of either gene was unable to recover the defect of ODV occlusion, suggesting that Bm133 and Bm134 are indispensable to the embedding of ODVs into polyhedra. Confocal microscopy analysis showed that Bm133 and Bm134 distributed throughout the whole cell during viral infection and Bm134 concentrated on the mature polyhedra in lysed cells. These results suggest that although Bm133 and Bm134 are not essential for BV or ODV development, they play vital roles in polyhedra morphogenesis.
Assuntos
Nucleopoliedrovírus/genética , Fases de Leitura Aberta/genética , Montagem de Vírus/genética , Replicação Viral , Animais , Linhagem Celular , Técnicas de Inativação de Genes , Microscopia Eletrônica , Nucleocapsídeo/genética , Nucleocapsídeo/fisiologia , Nucleocapsídeo/ultraestrutura , Nucleopoliedrovírus/fisiologia , Nucleopoliedrovírus/ultraestrutura , Células Sf9 , Proteínas Virais , Liberação de VírusRESUMO
After ingestion of occlusion bodies, the occlusion-derived viruses (ODVs) of the baculoviruses establish the first round of infection within the larval host midgut cells. Several ODV envelope proteins, called per os infectivity factors (PIFs), have been shown to be essential for oral infection. Eight PIFs have been identified to date, including P74, PIFs 1-6 and Ac110. At least six PIFs, P74, PIFs 1-4 and PIF6, together with three other ODV-specific proteins, Ac5, P95 (Ac83) and Ac108, have been reported to form a complex on the ODV surface. In this study, in order to understand the interactions of these PIFs, the direct protein-protein interactions of the nine components of the Autographa californica multiple nucleopolyhedrovirus PIF complex were investigated using yeast two-hybrid (Y2H) screening combined with bimolecular fluorescence complementation (BiFC) assay. Six direct interactions, comprising PIF1-PIF2, PIF1-PIF3, PIF1-PIF4, PIF1-P95, PIF2-PIF3 and PIF3-PIF4, were identified in the Y2H analysis, and these results were further verified by BiFC. For P74, PIF6, Ac5 and Ac108, no direct interaction was identified. P95 (Ac83) was identified to interact with PIF1, and further Y2H analysis of the truncation and deletion mutants showed that the predicted P95 chitin-binding domain and amino acids 100-200 of PIF1 were responsible for P95 interaction with PIF1. Furthermore, a summary of the protein-protein interactions of PIFs reported so far, comprising 10 reciprocal interactions and two self-interactions, is presented, which will facilitate our understanding of the characteristics of the PIF complex.
Assuntos
Baculoviridae/fisiologia , Mapeamento de Interação de Proteínas , Proteínas do Envelope Viral/metabolismo , Animais , Imagem Óptica , Células Sf9 , Spodoptera , Técnicas do Sistema de Duplo-HíbridoRESUMO
Bombyx mori nucleopolyhedrovirus (BmNPV) is a major pathogen that specifically infects the domestic silkworm and causes serious economic loss to sericulture around the world. The function of BmNPV Bm59 gene in the viral life cycle is inconclusive. To investigate the role of Bm59 during viral infection, the transcription initiation site and temporal expression of Bm59 were analyzed, and Bm59-knockout virus was generated through homologous recombination in Escherichia coli. The results showed that Bm59 is an early transcription gene with an atypia early transcriptional start motif. Budded virion (BV) production and DNA replication in the BmN cells transfected with the Bm59-knockout virus bacmid were similar to those in the cells transfected with the wild-type virus. Electron microscopy revealed that the occlusion-derived virus can be produced in cells infected with the Bm59-knockout virus. These results indicated that Bm59 is an early gene and is not essential for viral replication or assembly of BmNPV. These findings suggested that non-essential gene (Bm59) remained in the viral genome, which may interact with other viral/host genes in a certain situation.
Assuntos
Bombyx/virologia , Nucleopoliedrovírus/genética , Proteínas Virais/genética , Montagem de Vírus/genética , Animais , Linhagem Celular , Replicação do DNA/genética , Regulação Viral da Expressão Gênica/genética , Técnicas de Inativação de Genes/métodos , Microscopia Eletrônica/métodos , Sítio de Iniciação de Transcrição/fisiologia , Transcrição Gênica/genética , Transfecção/métodos , Replicação Viral/genéticaRESUMO
BACKGROUND: Bombyx mori nucleopolyhedrovirus (BmNPV) orf64 (Bm64, a homologue of ac78) is a core baculovirus gene. Recently, Li et al. reported that Ac78 was not essential for budded viruses (BVs) production and occlusion-derived viruses (ODVs) formation (Virus Res 191:70-82, 2014). Conversely, Tao et al. demonstrated that Ac78 was localized to the BV and ODV envelopes and was required for BV production and ODV formation (J Virol 87:8441-50, 2013). In this study, the function of Bm64 was characterized to determine the role of Bm64 in the BmNPV infection cycle. METHOD: The temporal expression of Bm64 was examined using total RNA extracted from BmNPV-infected BmN cells at different time points by reverse-transcription PCR (RT-PCR) and 5' RACE analysis. To determine the functions of Bm64 in viral replication and the viral phenotype throughout the viral life cycle, a deletion virus (vBm(64KO)) was generated via homologous recombination in Escherichia coli. Viral replication and BV production were determined by real-time PCR. Electron microscopy was used to detect virion morphogenesis. The subcellular localization of Bm64 was determined by microscopy, and per os infectivity was used to determine its role in the baculovirus oral infection cycle. RESULTS: Viral plaque and titer assay results showed that a few infectious BVs were produced by vBm(64KO), suggesting that deletion of Bm64 affected BV production. Viral DNA replication was detected and polyhedra were observed in vBm(64KO)-transfected cells. Microscopy analysis revealed that Bm64 was predominantly localized to the ring zone of the nuclei during the infection cycle. Electron microscopy showed that Bm64 was not essential for the formation of ODVs or the subsequent occlusion of ODV into polyhedra. The per os infectivity results showed that the polyhedra of vBm(64KO) were unable to infect silkworm larvae. CONCLUSION: In conclusion, our results suggest that Bm64 plays an important role in BV production and per os infection, but is not required for viral DNA replication or ODV maturation.
Assuntos
Bombyx/virologia , Nucleopoliedrovírus/fisiologia , Proteínas Virais/metabolismo , Liberação de Vírus , Animais , Linhagem Celular , Deleção de Genes , Perfilação da Expressão Gênica , Microscopia Eletrônica de Transmissão , Nucleopoliedrovírus/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Ensaio de Placa Viral , Proteínas Virais/genética , Vírion/ultraestruturaRESUMO
Baculoviruses have been known to induce hyperactive behavior in their lepidopteran hosts for over a century. As a typical lepidopteran insect, the silkworm Bombyx mori displays enhanced locomotor activity (ELA) following infection with B. mori nucleopolyhedrovirus (BmNPV). Some investigations have focused on the molecular mechanisms underlying this abnormal hyperactive wandering behavior due to the virus; however, there are currently no reports about B. mori. Based on previous studies that have revealed that behavior is controlled by the central nervous system, the transcriptome profiles of the brains of BmNPV-infected and non-infected silkworm larvae were analyzed with the RNA-Seq technique to reveal the changes in the BmNPV-infected brain on the transcriptional level and to provide new clues regarding the molecular mechanisms that underlies BmNPV-induced ELA. Compared with the controls, a total of 742 differentially expressed genes (DEGs), including 218 up-regulated and 524 down-regulated candidates, were identified, of which 499, 117 and 144 DEGs could be classified into GO categories, KEGG pathways and COG annotations by GO, KEGG and COG analyses, respectively. We focused our attention on the DEGs that are involved in circadian rhythms, synaptic transmission and the serotonin receptor signaling pathway of B. mori. Our analyses suggested that these genes were related to the locomotor activity of B. mori via their essential roles in the regulations of a variety of behaviors and the down-regulation of their expressions following BmNPV infection. These results provide new insight into the molecular mechanisms of BmNPV-induced ELA.
Assuntos
Bombyx/virologia , Encéfalo/metabolismo , Interações Hospedeiro-Patógeno/fisiologia , Atividade Motora , Nucleopoliedrovírus , Animais , Perfilação da Expressão Gênica , Atividade Motora/fisiologia , Reação em Cadeia da Polimerase , TranscriptomaRESUMO
Bombyx mori nucleopolyhedrovirus (BmNPV) BmP95 is a highly conserved gene that is found in all of the baculovirus genomes sequenced to date and is also found in nudiviruses. To investigate the role of BmP95 in virus infection in vitro, a BmP95 deletion virus (vBmP95-De) was generated by homologous recombination in Escherichia coli. Fluorescence and light microscopy and titration analysis indicated that the BmP95 deletion bacmid led to a defect in production of infectious budded virus (BV). However, deletion of BmP95 did not affect viral DNA replication. Electron microscopy showed that masses of aberrant tubular structures were present in cells transfected with the BmP95 deletion bacmid, indicating that deletion of BmP95 affected assembly of the nucleocapsid. This defect could be rescued by insertion of full-length BmP95 into the polyhedrin locus of the BmP95-knockout bacmid but not the N-terminal domain of BmP95. Together, these results showed that full-length BmP95 is essential for BV production and is required for nucleocapsid assembly.
Assuntos
Bombyx/virologia , Genes Essenciais , Nucleocapsídeo/metabolismo , Nucleopoliedrovírus/metabolismo , Proteínas Virais/metabolismo , Liberação de Vírus , Animais , Linhagem Celular , Replicação do DNA , DNA Viral/biossíntese , DNA Viral/genética , Deleção de Genes , Microscopia Eletrônica , Nucleopoliedrovírus/genética , Transfecção , Proteínas Virais/genética , Montagem de VírusRESUMO
Lipases play crucial roles in food digestion by degrading dietary lipids into free fatty acids and glycerols. The domesticated silkworm (Bombyx mori) has been widely used as an important Lepidopteran model for decades. However, little is known about the lipase gene family in the silkworm, especially their hydrolytic activities as digestive enzymes. In this study, a total of 38 lipase genes were identified in the silkworm genome. Phylogenetic analysis indicated that they were divided into three major groups. Twelve lipases were confirmed to be expressed in the midgut at both transcriptional and translational levels. They were grouped into the same gene cluster, suggesting that they could have similar physiological functions. Quantitative real-time PCR (qRT-PCR) analyses indicated that lipases were mainly expressed in anterior and middle midgut regions, and their expression levels varied greatly along the length of midgut. A majority of lipases were down-regulated in the midgut when larvae stopped feeding. However, a unique lipase gene (Bmlip10583) showed low expression level during feeding stage, but it was significantly up-regulated during the larvae-pupae transition. These results demonstrated that expression of silkworm lipases was spatially and temporally regulated in the midgut during larval development. Taken together, our results provide a fundamental research of the lipase gene family in the silkworm.
Assuntos
Bombyx/enzimologia , Proteínas de Insetos/biossíntese , Lipase/biossíntese , Animais , Bombyx/genética , Sistema Digestório/enzimologia , Expressão Gênica , Estudo de Associação Genômica Ampla/métodos , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Larva/enzimologia , Larva/genética , Lipase/genética , Lipase/metabolismo , Filogenia , Processamento de Proteína Pós-Traducional , Proteômica/métodos , TranscriptomaRESUMO
Insect midgut plays a central role in food digestion and nutrition absorption. Larval silkworm midgut could be divided into 3 distinct regions based on their morphological colors. However, it remains rudimentary of regional gene expression and physiological function in larval silkworm midgut. Through transcriptome sequencing of 3 midgut compartments, a comprehensive analysis of gene expression atlas along the anterior-posterior axis was conducted. Posterior midgut was found transcriptionally divergent from anterior and middle midgut. Differentially expressed gene analysis revealed the regional specialization of digestive enzyme production, transmembrane transport, chitin metabolism, and hormone regulation in different midgut regions. In addition, gene subsets of pan-midgut and region-specific transcription factors (TFs) along the length of midgut were also identified. The results suggested that homeobox TFs might play an essential role in transcriptional variations across the midgut. Altogether, our study provided the first fundamental resource to investigate physiological function and regulation mechanism in larval midgut compartmentalization.
Assuntos
Bombyx , Animais , Bombyx/genética , Bombyx/metabolismo , Quitina/metabolismo , Perfilação da Expressão Gênica , Hormônios/metabolismo , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Larva/genética , Larva/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , TranscriptomaRESUMO
Bombyx mori nucleopolyhedrovirus (BmNPV) is highly pathogenic to Bombyx mori, silkworm, which causes serious cytopathic effects (CPEs) during infection. However, the role of viral protein in the virus-induced CPEs remains unclear. Here, we discovered that BmNPV infection induced severe CPEs including titer-dependent cell floating and changes in cellular surface morphology. Further explorations revealed the involvement of F-like protein (Bm14), a viral envelope protein, in inducing cytotoxicity and detachment of adherent BmN cells, and its disruption significantly impaired the virus infection-mediated CPEs. Intriguingly, transcriptomic analysis identified the tight association of Bm14 deletion with the activation of cellular oxidative phosphorylation pathway, consistent with the elevated mitochondrial membrane potential (MMP) levels and ATP concentrations as well as reduced ROS levels. Collectively, our results characterized for the first time the novel role of Bm14 in accelerating viral-induced cytopathogenicity via suppressing the cellular oxidative phosphorylation levels and upregulating the ROS levels.
Assuntos
Bombyx/virologia , Efeito Citopatogênico Viral , Nucleopoliedrovírus/metabolismo , Fosforilação Oxidativa , Espécies Reativas de Oxigênio/metabolismo , Proteínas do Envelope Viral/fisiologia , Viroses/metabolismo , Animais , Linhagem Celular , Perfilação da Expressão Gênica , Interações entre Hospedeiro e Microrganismos , Mutação , RNA-Seq , Regulação para CimaRESUMO
The insect brain plays crucial roles in the regulation of growth and development and in all types of behavior. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis and high-performance liquid chromatography - electron spray ionization tandem mass spectrometry (ESI-MS/MS) shotgun to identify the proteome of the silkworm brain, to investigate its protein composition and to understand their biological functions. A total of 2210 proteins with molecular weights in the range of 5.64-1539.82 kDa and isoelectric points in the range of 3.78-12.55 were identified. These proteins were annotated according to Gene Ontology Annotation into the categories of molecular function, biological process and cellular component. We characterized two categories of proteins: one includes behavior-related proteins involved in the regulation of behaviors, such as locomotion, reproduction and learning; the other consists of proteins related to the development or function of the nervous system. The identified proteins were classified into 283 different pathways according to KEGG analysis, including the PI3K-Akt signaling pathway which plays a crucial role in mediating survival signals in a wide range of neuronal cell types. This extensive protein profile provides a basis for further understanding of the physiological functions in the silkworm brain.