Exploring bacterial community assembly in vadose and saturated zone soil for tailored bioremediation of a long-term hydrocarbon-contaminated site.

Indigenous soil microbial communities play a pivotal role in the in situ bioremediation of contaminated sites. However, research on the distribution characteristics of microbial communities at various soil depths remains limited. In particular, there is little information on the assembly of microbial communities, especially those with degradation potential, in the vadose and saturated zones of hydrocarbon-contaminated sites. In this study, 18 soil samples were collected from the vadose zone and saturated zone at a long-term hydrocarbon-contaminated site. The diversity, composition, and driving factors of assembly of the soil bacterial community were determined by high-throughput sequencing analysis. Species richness and diversity were significantly higher in the vadose zone soils than in the saturated zone soils. Significant differences in abundance at both the phylum and genus levels were observed between the two zones. Soil bacterial community assembly was driven by the combination of pollution stress and nutrients in the vadose zone but by nutrient limitations in the saturated zone. The abundance of dechlorinating bacteria was greater in the saturated zone soils than in the vadose zone soils. Compared with contaminant concentrations, nutrient levels had a more pronounced impact on the abundance of dechlorinating bacteria. In addition, the interactions among dechlorinating bacterial populations were stronger in the saturated zone soils than in the vadose zone soils. These findings underscore the importance of comprehensively understanding indigenous microbial communities, especially those with degradation potential, across different soil layers to devise specific, effective in situ bioremediation strategies for contaminated sites.

The Effect of Manure Application on Arsenic Mobilization and Methylation in Different Paddy Soils.

Organic matter plays an important role in controlling arsenic(As) release and transformation in soil, however, little is known about the effect of manure application on As behavior in soils with different As contents. In this study, waterlogged incubations using various As-contaminated paddy soils with manure amendment were conducted to investigate how manure application influence As mobilization and methylation in different paddy soils. The results indicated that manure application increased As release in paddy soils with high As (> 30 mg kg-1) contents. Moreover, our findings also showed that manure application increased the relative abundance of arsM-harboring Euryacheota and Planctomycetes at the phylum level and arsM-harbouring Methanocellaceae, Anaerolinea and Bellinea at genus level, thereby promoting As methylation. These results provide important insights for the significant variation in As mobilization and methylation in paddy soils amended with manure. Moreover, our results suggest that serious consideration should be given to the manure application in As-contaminated paddy soil.

Synergistic biodegradation of trichloroethylene-contaminated soil using Typha angustifolia L. and an anaerobic degrading bacterial consortium.

Plant-microorganism combined bioremediation is a highly efficient and environmentally sustainable method for the remediation of contaminated soils. Despite its potential, the synergistic effects of wetland plants and anaerobic microbial consortium on the removal of chlorinated hydrocarbons (CAHs) from soil remain inadequately understood. In this study, an anaerobic bacterial consortium, capable of completely dechlorinating trichloroethylene (TCE), was enriched and screened from long-term CAH-contaminated soil. Subsequently, the combined effects of the wetland plant Typha angustifolia L. and the anaerobic bacterial consortium on the removal of TCE from soil were investigated, along with the underlying microbial mechanisms. The results demonstrated that the anaerobic bacterial consortium was able to completely convert 0.5 mM TCE to vinyl chloride (VC) within 7 days, and subsequently degrade VC to ethylene within 48 days. The integration of Typha angustifolia L. with the anaerobic bacterial consortium significantly enhanced both the removal and complete dechlorination of TCE from the soil compared to either treatment alone. Furthermore, this combination substantially increased the diversity and richness of soil bacterial communities, enriched dechlorinating microorganisms, and elevated the relative abundance of dehalogenating enzymes and peroxidases involved in pollutant degradation. In addition, the combination resulted in a more complex soil bacterial community, closer microbial interactions, and a more stable microbial co-occurrence network. This study extends the scope of plant-microorganism combined bioremediation for CAH- contaminated soils.

Vegetation restoration improved aggregation stability and aggregated-associated carbon preservation in the karst areas of Guizhou Province, southwest China.

Background: The change in the soil carbon bank is closely related to the carbon dioxide in the atmosphere, and the vegetation litter input can change the soil organic carbon content. However, due to various factors, such as soil type, climate, and plant species, the effects of vegetation restoration on the soil vary. Currently, research on aggregate-associated carbon has focused on single vegetation and soil surface layers, and the changes in soil aggregate stability and carbon sequestration under different vegetation restoration modes and in deeper soil layers remain unclear. Therefore, this study aimed to explore the differences and relationships between stability and the carbon preservation capacity (CPC) under different vegetation restoration modes and to clarify the main influencing factors of aggregate carbon preservation. Methods: Grassland (GL), shrubland (SL), woodland (WL), and garden plots (GP) were sampled, and they were compared with farmland (FL) as the control. Soil samples of 0-40 cm were collected. The soil aggregate distribution, aggregate-associated organic carbon concentration, CPC, and stability indicators, including the mean weight diameter (MWD), fractal dimension (D), soil erodibility (K), and geometric mean diameter (GMD), were measured. Results: The results showed that at 0-40 cm, vegetation restoration significantly increased the >2 mm aggregate proportions, aggregate stability, soil organic carbon (SOC) content, CPC, and soil erosion resistance. The >2 mm fractions of the GL and SL were at a significantly greater proportion at 0-40 cm than that of the other vegetation types but the CPC was only significantly different between 0 and 10 cm when compared with the other vegetation types (P < 0.05). The >2 mm aggregates showed a significant positive correlation with the CPC, MWD, and GMD (P < 0.01), and there was a significant negative correlation with the D and K (P < 0.05). The SOC and CPC of all the vegetation types were mainly distributed in the 0.25-2 mm and <0.25 mm aggregate fractions. The MWD, GMD, SOC, and CPC all gradually decreased with increasing soil depth. Overall, the effects of vegetation recovery on soil carbon sequestration and soil stability were related to vegetation type, aggregate particle size, and soil depth, and the GL and SL restoration patterns may be more suitable in this study area. Therefore, to improve the soil quality and the sequestration of organic carbon and reduce soil erosion, the protection of vegetation should be strengthened and the policy of returning farmland to forest should be prioritized.

[Evolution Characteristics of Soil Active Organic Carbon and Carbon Pool Management Index Under Vegetation Restoration in Karst Area].

Vegetation restoration affects the carbon cycle of terrestrial ecosystems by changing the rate of carbon input and conversion. In order to explore the evolution characteristics of soil active organic carbon components and carbon pool management index during vegetation restoration in karst areas, the soil of a grassland sequence(5, 10, 15, and 20 a), shrub sequence(5, 10, 15, and 20 a), and garden sequence(5, 10, and 15 a) in a karst area was taken as the research object, and the adjacent farmland was taken as the control(CK). The effects of different vegetation restoration years on the evolution of soil organic carbon(SOC), readily oxidizable organic carbon(ROC333, ROC167, and ROC33 were all soil active organic carbon that could be oxidized by 333, 167, and 33 mmol·L-1 KMnO4), microbial biomass carbon(MBC), dissolved organic carbon(DOC), and carbon pool management index(CPMI) were analyzed. The results showed that compared with that of CK, the average grassland, shrub, and garden SOC contents in the 0-40 cm soil layer increased by 70.77%, 114.40%, and 50.17%, respectively. In the 0-20 cm soil layer, with the increase in restoration years, the SOC content of the grassland sequence and garden sequence increased first and then decreased, and that of the shrub sequence increased first, then decreased, and then increased again. ROC333, ROC167, and ROC33 were consistent with the SOC change trend of the corresponding sequence. In the 20-40 cm soil layer, the change trend of ROC333, ROC167, and ROC33 of each sequence was inconsistent with the SOC of the corresponding sequence. In the 0-40 cm soil layer, the MBC content of the grassland sequence decreased first, then increased, and then decreased, and the maximum value of MBC in each soil layer was in G15. The shrub sequence in the 0-10 cm soil layer increased first, then decreased, and then increased, and in the 10-40 cm soil layer it increased first and then decreased. The garden sequence increased first and then decreased in the 0-30 cm soil layer and gradually increased in the 30-40 cm soil layer. Kos of the three sequences decreased first, then increased, and then decreased, whereas L and LI showed the opposite of Kos. CPI increased first and then decreased; the CPMI of the grassland and garden sequences increased first and then decreased, whereas the CPMI of the shrub sequence increased first, then decreased, and then increased again. The contents of SOC, ROC333, ROC167, ROC33, and MBC and the annual growth of Kos were shrub>grassland>orchard, and the annual growth of DOC and CPMI were orchard>grassland>shrub. The contents of SOC and its components in the three sequences decreased with the increase in soil layer and had obvious surface aggregation. Redundancy analysis showed that alkali-hydrolyzable nitrogen(AN) was the main environmental factor affecting soil active organic carbon components and soil organic carbon pool under the vegetation restoration in the karst area. In summary, soil active organic carbon components and CPMI evolved with vegetation restoration years. Different vegetation restorations could increase the content of SOC and its components in karst areas to a certain extent, and shrub restoration promotes the accumulation of SOC.

[Vulnerability assessment of agro-ecological environment in Karst mountain based on set pair analysis model of grey trigonometrically whitening weight]. / 基于灰色三角白化权集对分析模型的喀斯特山区农业生态环境脆弱性评价.

Ecological vulnerability is a hot issue in the study of global change and sustainable development. Understanding the vulnerability of agro-ecological environment is conducive to rational utilization of regional agricultural resources, which could put forward effective measures for prote-cting agro-ecological environment. Given that the evaluation of agricultural eco-environment vulnerability generally does not consider the relationships among different indicators in different evaluation levels, we used the grey trigonometrically whitening weight set pair analysis (SPA) model to evaluate the vulnerability of agricultural eco-environment in Karst mountain by selecting 11 indicators such as population density, per capita arable land area and per capita afforestation area from the external vulnerability of ecological environment. The results showed that the vulnerability degree of agro-ecological environment in the study area was very high, mainly at the extreme, high and medium vulnerability grades. The proportion of extremely, highly, moderately, mildly and slightly vulnerable areas was 32.4%, 14.1%, 17.7%, 23.6% and 12.2% respectively. This result was consistent with the status of agricultural ecological environment vulnerability in the study area. It was feasible to evaluate the vulnerability of agro-ecosystem with the SPA Model of grey trigonometrically whitening weight, which provided a new method for evaluating agricultural ecological environment vulnerability.