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1.
Sheng Wu Gong Cheng Xue Bao ; 17(3): 288-92, 2001 May.
Artigo em Zh | MEDLINE | ID: mdl-11517602

RESUMO

An efficient and rapid gene transformation system of rice mediated by Agrobacterium tumefaciens was used. Calli induced from immature and mature embryos of Zhonghua No. 11, a japonic rice variety, were cultured with the A. tumefaciens strain EHA105 harboring the superbinary plasmid pDsBar1300 or pUBITs separately, and more than 400 independent transgenic lines inserted Ds element or Ac fragment were obtained. Some visible mutants in T0 or T1 generation were found, consisting of disease resistance, albino, dwarf, male sterile, chlorosis, early heading, late heading, stripe, etc. From the phenotype analysis, a few mutants such as dwarf and male sterile seemed to be linked to the Basta resistance and the transposon.


Assuntos
Elementos de DNA Transponíveis , Mutação , Oryza/genética , Zea mays/genética , Agrobacterium tumefaciens/genética , Plantas Geneticamente Modificadas , Reação em Cadeia da Polimerase , Técnica de Amplificação ao Acaso de DNA Polimórfico
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