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Chemical pesticides and fertilizers are used in agricultural production worldwide to prevent damage from plant pathogenic microorganisms, insects, and nematodes, to minimize crop losses and to preserve crop quality. However, the use of chemical pesticides and fertilizers can severely pollute soil, water, and air, posing risks to the environment and human health. Consequently, developing new, alternative, environment-friendly microbial soil treatment interventions for plant protection and crop yield increase has become indispensable. Members of the filamentous fungal genus Trichoderma (Ascomycota, Sordariomycetes, Hypocreales) have long been known as efficient antagonists of plant pathogenic microorganisms based on various beneficial traits and abilities of these fungi. This minireview aims to discuss the advances in the field of Trichoderma-containing multicomponent microbiological inoculants based on recent experimental updates. Trichoderma strains can be combined with each other, with other fungi and/or with beneficial bacteria. The development and field performance of such inoculants will be addressed, focusing on the complementarity, synergy, and compatibility of their microbial components.
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Inoculantes Agrícolas , Praguicidas , Trichoderma , Humanos , Fertilizantes , SoloRESUMO
Fungal parasitism depends on the ability to invade host organisms and mandates adaptive cell wall remodeling to avoid detection and defense reactions by the host. All plant and human pathogens share invasive strategies, which aid to escape the chitin-triggered and chitin-targeted host immune system. Here we describe the full spectrum of the chitin/chitosan-modifying enzymes in the mycoparasite Trichoderma atroviride with a central role in cell wall remodeling. Rapid adaption to a variety of growth conditions, environmental stresses and host defense mechanisms such as oxidative stress depend on the concerted interplay of these enzymes and, ultimately, are necessary for the success of the mycoparasitic attack. To our knowledge, we provide the first in class description of chitin and associated glycopolymer synthesis in a mycoparasite and demonstrate that they are essential for biocontrol. Eight chitin synthases, six chitin deacetylases, additional chitinolytic enzymes, including six chitosanases, transglycosylases as well as accessory proteins are involved in this intricately regulated process. Systematic and biochemical classification, phenotypic characterization and mycoparasitic confrontation assays emphasize the importance of chitin and chitosan assembly in vegetative development and biocontrol in T. atroviride. Our findings critically contribute to understanding the molecular mechanism of chitin synthesis in filamentous fungi and mycoparasites with the overarching goal to selectively exploit the discovered biocontrol strategies.
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Quitina/metabolismo , Quitosana/metabolismo , Trichoderma/metabolismo , Parede Celular/metabolismo , Quitina/fisiologia , Quitina Sintase/metabolismo , Regulação Fúngica da Expressão Gênica/genética , Glicosídeo Hidrolases , Filogenia , Plantas/metabolismo , Trichoderma/crescimento & desenvolvimento , Trichoderma/patogenicidadeRESUMO
Recombination shapes the evolutionary trajectory of populations and plays an important role in the faithful transmission of chromosomes during meiosis. Levels of sexual reproduction and recombination are important properties of host-pathogen interactions because the speed of antagonistic co-evolution depends on the ability of hosts and pathogens to generate genetic variation. However, our understanding of the importance of recombination is limited because large taxonomic groups remain poorly investigated. Here, we analyze recombination rate variation in the basidiomycete fungus Armillaria ostoyae, which is an aggressive pathogen on a broad range of conifers and other trees. We analyzed a previously constructed, dense genetic map based on 198 single basidiospore progeny from a cross. Progeny were genotyped at a genome-wide set of single-nucleotide polymorphism (SNP) markers using double digest restriction site associated DNA sequencing. Based on a linkage map of on 11,700 SNPs spanning 1007.5 cM, we assembled genomic scaffolds into 11 putative chromosomes of a total genome size of 56.6 Mb. We identified 1984 crossover events among all progeny and found that recombination rates were highly variable along chromosomes. Recombination hotspots tended to be in regions close to the telomeres and were more gene-poor than the genomic background. Genes in proximity to recombination hotspots were encoding on average shorter proteins and were enriched for pectin degrading enzymes. Our analyses enable more powerful population and genome-scale studies of a major tree pathogen.
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Armillaria , Genoma Fúngico , Recombinação Genética , Armillaria/genética , Cromossomos Fúngicos , Florestas , Ligação Genética , Polimorfismo de Nucleotídeo ÚnicoRESUMO
Filamentous fungi exhibit a broad spectrum of heritable growth patterns and morphological variations reflecting the adaptation of the different species to distinct ecological niches. But also within species, isolates show considerable variation in growth rates and other morphological characteristics. The genetic basis of this intraspecific variation in mycelial growth and morphology is currently poorly understood. By chance, a growth mutant in the root rot pathogen Armillaria ostoyae was discovered. The mutant phenotype was characterized by extremely compact and slow growth, as well as shorter aerial hyphae and hyphal compartments in comparison to the wildtype phenotype. Genetic analysis revealed that the abnormal phenotype is caused by a recessive mutation, which segregates asa single locus in sexual crosses. In order to identify the genetic basis of the mutant phenotype, we performed a quantitative trait locus (QTL) analysis. A mapping population of 198 haploid progeny was genotyped at 11,700 genome-wide single nucleotide polymorphisms (SNPs) making use of double digest restriction site associated DNA sequencing (ddRADseq). In accordance with the genetic analysis, a single significant QTL was identified for the abnormal growth phenotype. The QTL confidence interval spans a narrow, gene dense region of 87kb in the A. ostoyae genome which contains 37 genes. Overall, our study reports the first high-density genetic map for an Armillaria species and shows its successful application in forward genetics by resolving the genetic basis of a mutant phenotype with a severe defect in hyphal growth.
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Armillaria/genética , Armillaria/citologia , Armillaria/crescimento & desenvolvimento , Mapeamento Cromossômico , Cruzamentos Genéticos , Elementos de DNA Transponíveis , Genes Fúngicos , Genótipo , Mutação , Pinus sylvestris/microbiologia , Doenças das Plantas/microbiologia , Raízes de Plantas/microbiologia , Locos de Características QuantitativasRESUMO
[This corrects the article DOI: 10.3389/fbioe.2023.1189640.].
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Genes involved in mycoremediation were identified by comparative genomics analysis in 10 armillarioid species and selected groups of white-rot Basidiomycota (14) and soft-rot Ascomycota (12) species to confine the distinctive bioremediation capabilities of the armillarioids. The genomes were explored using phylogenetic principal component analysis (pPCA), searching for genes already documented in a biocatalysis/biodegradation database. The results underlined a distinct, increased potential of aromatics-degrading genes/enzymes in armillarioids, with particular emphasis on a high copy number and diverse spectrum of benzoate 4-monooxygenase [EC:1.14.14.92] homologs. In addition, other enzymes involved in the degradation of various monocyclic aromatics were more abundant in the armillarioids than in the other white-rot basidiomycetes, and enzymes involved in the degradation of polycyclic aromatic hydrocarbons (PAHs) were more prevailing in armillarioids and other white-rot species than in soft-rot Ascomycetes. Transcriptome profiling of A. ostoyae and A. borealis isolates confirmed that several genes involved in the degradation of benzoates and other monocyclic aromatics were distinctively expressed in the wood-invading fungal mycelia. Data were consistent with armillarioid species offering a more powerful potential in degrading aromatics. Our results provide a reliable, practical solution for screening the likely fungal candidates for their full biodegradation potential, applicability, and possible specialization based on their genomics data.
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Armillaria ostoyae, a species among the destructive forest pathogens from the genus Armillaria, causes root rot disease on woody plants worldwide. Efficient control measures to limit the growth and impact of this severe underground pathogen are under investigation. In a previous study, a new soilborne fungal isolate, Trichoderma atroviride SZMC 24276 (TA), exhibited high antagonistic efficacy, which suggested that it could be utilized as a biocontrol agent. The dual culture assay results indicated that the haploid A. ostoyae-derivative SZMC 23085 (AO) (C18/9) is highly susceptible to the mycelial invasion of TA. In the present study, we analyzed the transcriptome of AO and that of TA in in vitro dual culture assays to test the molecular arsenal of Trichoderma antagonism and the defense mechanisms of Armillaria. We conducted time-course analysis and functional annotation and analyzed enriched pathways and differentially expressed genes including biocontrol-related candidate genes from TA and defense-related candidate genes from AO. The results indicated that TA deployed several biocontrol mechanisms when confronted with AO. In response, AO initiated multiple defense mechanisms to protect against the fungal attack. To our knowledge, the present study offers the first transcriptome analysis of a biocontrol fungus attacking AO. Overall, this study provides insights that aid the further exploration of plant pathogen-biocontrol agent interaction mechanisms. IMPORTANCE Armillaria species can survive for decades in the soil on dead woody debris, develop rapidly under favorable conditions, and harmfully infect newly planted forests. Our previous study found Trichoderma atroviride to be highly effective in controlling Armillaria growth; therefore, our current work explored the molecular mechanisms that might play a key role in Trichoderma-Armillaria interactions. Direct confrontation assays combined with time course-based dual transcriptome analysis provided a reliable system for uncovering the interactive molecular dynamics between the fungal plant pathogen and its mycoparasitic partner. Furthermore, using a haploid Armillaria isolate allowed us to survey the deadly prey-invading activities of the mycoparasite and the ultimate defensive strategies of its prey. Our current study provides detailed insights into the essential genes and mechanisms involved in Armillaria defense against Trichoderma and the genes potentially involved in the efficiency of Trichoderma to control Armillaria. In addition, using a sensitive haploid Armillaria strain (C18/9), with its complete genome data already available, also offers the opportunity to test possible variable molecular responses of Armillaria ostoyae toward diverse Trichoderma isolates with various biocontrol abilities. Initial molecular tests of the dual interactions may soon help to develop a targeted biocontrol intervention with mycoparasites against plant pathogens.
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Armillaria , Trichoderma , Armillaria/genética , RNA-Seq , Haploidia , Plantas/genéticaRESUMO
The fungal genus Armillaria contains necrotrophic pathogens and some of the largest terrestrial organisms that cause tremendous losses in diverse ecosystems, yet how they evolved pathogenicity in a clade of dominantly non-pathogenic wood degraders remains elusive. Here we show that Armillaria species, in addition to gene duplications and de novo gene origins, acquired at least 1,025 genes via 124 horizontal gene transfer events, primarily from Ascomycota. Horizontal gene transfer might have affected plant biomass degrading and virulence abilities of Armillaria, and provides an explanation for their unusual, soft rot-like wood decay strategy. Combined multi-species expression data revealed extensive regulation of horizontally acquired and wood-decay related genes, putative virulence factors and two novel conserved pathogenicity-induced small secreted proteins, which induced necrosis in planta. Overall, this study details how evolution knitted together horizontally and vertically inherited genes in complex adaptive traits of plant biomass degradation and pathogenicity in important fungal pathogens.
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Armillaria , Armillaria/genética , Armillaria/metabolismo , Biomassa , Transferência Genética Horizontal , Ecossistema , PlantasRESUMO
The green and facile synthesis of metallic silver nanoparticles (AgNPs) is getting tremendous attention for exploring superior applications because of their small dimensions and shape. AgNPs are already proven materials for superior coloration, biocidal, thermal, UV-protection, and mechanical performance. Originally, some conventional chemical-based reducing agents were used to synthesize AgNPs, but these posed potential risks, especially for enhanced toxicity. This became a driving force to innovate plant-based sustainable and green metallic nanoparticles (NPs). Moreover, the synthesized NPs using plant-based derivatives could be tuned and regulated to achieve the required shape and size of the AgNPs. AgNPs synthesized from naturally derived materials are safe, economical, eco-friendly, facile, and convenient, which is also motivating researchers to find greener routes and viable options, utilizing various parts of plants like flowers, stems, heartwood, leaves and carbohydrates like chitosan to meet the demands. This article intends to provide a comprehensive review of all aspects of AgNP materials, including green synthesis methodology and mechanism, incorporation of advanced technologies, morphological and elemental study, functional properties (coloration, UV-protection, biocidal, thermal, and mechanical properties), marketing value, future prospects and application, especially for the last 20 years or more. The article also includes a SWOT (Strengths, weaknesses, opportunities, and threats) analysis regarding the use of AgNPs. This report would facilitate the industries and consumers associated with AgNP synthesis and application through fulfilling the demand for sustainable, feasible, and low-cost product manufacturing protocols and their future prospects.
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(1) Background: Bacillus velezensis and Bacillus amyloliquefaciens are closely related members of the "operational group B. amyloliquefaciens", a taxonomical unit above species level within the "Bacillus subtilis species complex". They have similar morphological, physiological, biochemical, phenotypic, and phylogenetic characteristics. Thus, separating these two taxa from each another has proven to be difficult to implement and could not be pushed easily into the line of routine analyses. (2) Methods: The aim of this study was to determine whether whole FAME profiling could be used to distinguish between these two species, using both type strains and environmental isolates. Initially, the classification was determined by partial sequences of the gyrA and rpoB genes and the classified isolates and type strains were considered as samples to develop the identification method, based on FAME profiles. (3) Results: The dissimilarities in 16:0, 17:0 iso, and 17:0 FA components have drawn a distinction between the two species and minor differences in FA 14:0, 15:0 iso, and 16:0 iso were also visible. The statistical analysis of the FA profiles confirmed that the two taxa can be distinguished into two separate groups, where the isolates are identified without misreading. (4) Conclusions: Our study proposes that the developed easy and fast-automated identification tool based on cellular FA profiles can be routinely applied to distinguish B. velezensis and B. amyloliquefaciens.
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The geographical environment fundamentally influences the transport and deposition of sediments, including microplastics. In addition, the socioeconomic differences inherent in transboundary catchments result in various waste management strategies among the different countries influencing the input of microplastics into rivers. The catchment of the Tisza River in Central Europe is shared by five countries with different economic statuses and wastewater treatment practices. The aim of this research is to study the spatial changes in microplastic debris deposition along the Tisza and its main tributaries. The mean number of microplastic particles in the sediments of the Tisza was 3177 ± 1970 items/kg, while 3808 ± 1605 items/kg were counted in the sediments of the tributaries. Most of the particles were fibres, indicating the dominance of municipal wastewater input; this is especially the case in the upstream sub-catchments, where there are low degrees of wastewater management. The highest amount of microplastics was found in the sediments of the most-upstream section, where a low number of households are connected to wastewater treatment plants. Thus, it is hypothesized that suburban areas where high population densities and improper waste management co-exist may contribute to the direct input of microplastics into river systems and the development of local microplastic contamination hotspots. In addition, a high microplastic concentration was measured at the furthest downstream section, resulting from the decreased flow velocity related to impoundment by a dam. The efficiency of the microplastic trapping of the various depositionary forms varies along the river, suggesting various influencing factors and the complexity of the process. The higher concentration of microplastics observed in the tributaries compared to that observed in sediments of the main stream may reflect the importance of local sources and the complexity of source-to-sink relations for microplastic routes through the fluvial system; these processes do not always reflect progressive downstream increases.
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Hydrodynamic cavitation treatment was used for the functional inactivation of quorum-sensing lactone molecules of Pseudomonas aeruginosa. Hydroxyl radicals formed as well as the shear effects during the cavitation process induced the inactivation of the signal molecules through hydrolysis reaction coupled with bacterial destruction. Concentration of two different types of homoserine lactones (HSL) molecules was tested after the treatment at various rotational speeds. It was found that the strongest effects can be achieved at speeds > 2000 rpm. This value is considered as an onset speed of dominant cavitation, and it is in agreement with literature data. The experimental trends were in agreement with the calculations based on the finite element modelling, which show a significant increase in average shear stress at higher rotational speeds. Overall, the work has demonstrated the possible effects of hydrodynamic cavitation on the quorum-sensing molecules of Pseudomonas aeruginosa for the first time.
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Pseudomonas aeruginosa , Purificação da Água , 4-Butirolactona , Hidrodinâmica , Lactonas , Percepção de QuorumRESUMO
Wood-decaying Basidiomycetes are among the most efficient degraders of plant cell walls, making them key players in forest ecosystems, global carbon cycle, and in bio-based industries. Recent insights from -omics data revealed a high functional diversity of wood-decay strategies, especially among the traditional white-rot and brown-rot dichotomy. We examined the mechanistic bases of wood-decay in the conifer-specialists Armillaria ostoyae and Armillaria cepistipes using transcriptomic and proteomic approaches. Armillaria spp. (Fungi, Basidiomycota) include devastating pathogens of temperate forests and saprotrophs that decay wood. They have been discussed as white-rot species, though their response to wood deviates from typical white-rotters. While we observed an upregulation of a diverse suite of plant cell wall degrading enzymes, unlike white-rotters, they possess and express an atypical wood-decay repertoire in which pectinases and expansins are enriched, whereas lignin-decaying enzymes (LDEs) are generally downregulated. This combination of wood decay genes resembles the soft-rot of Ascomycota and appears widespread among Basidiomycota that produce a superficial white rot-like decay. These observations are consistent with ancestral soft-rot decay machinery conserved across asco- and Basidiomycota, a gain of efficient lignin-degrading ability in white-rot fungi and repeated, complete, or partial losses of LDE encoding gene repertoires in brown- and secondarily soft-rot fungi.
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Armillarioids, including the genera Armillaria, Desarmillaria and Guyanagaster, represent white-rot specific fungal saprotrophs with soilborne pathogenic potentials on woody hosts. They propagate in the soil by root-like rhizomorphs, connecting between susceptible root sections of their hosts, and often forming extended colonies in native forests. Pathogenic abilities of Armillaria and Desarmillaria genets can readily manifest in compromised hosts, or hosts with full vigour can be invaded by virulent mycelia when exposed to a larger number of newly formed genets. Armillaria root rot-related symptoms are indicators of ecological imbalances in native forests and plantations at the rhizosphere levels, often related to abiotic environmental threats, and most likely unfavourable changes in the microbiome compositions in the interactive zone of the roots. The less-studied biotic impacts that contribute to armillarioid host infection include fungi and insects, as well as forest conditions. On the other hand, negative biotic impactors, like bacterial communities, antagonistic fungi, nematodes and plant-derived substances may find applications in the environment-friendly, biological control of armillarioid root diseases, which can be used instead of, or in combination with the classical, but frequently problematic silvicultural and chemical control measures.
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Lingering in forests around the world, some of the largest and oldest terrestrial organisms on earth hide in plain sight. In this Quick Guide, Sipos et al. shed light on the biology of the Armillaria fungi.
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Armillaria/fisiologia , Doenças das Plantas/microbiologia , Raízes de Plantas/microbiologia , Armillaria/classificação , Armillaria/patogenicidade , Especificidade de Hospedeiro , Interações Hospedeiro-PatógenoRESUMO
In the version of this Article originally published, it was incorrectly stated that "16,687 protein-coding genes were inferred for the most recent common ancestor (MRCA) of Armillaria"; the value was incorrect and it should have read "15,787". This has now been corrected.
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SOI3 was identified by a mutation, soi3-1, that suppressed a mutant trans-Golgi network (TGN) localization signal in the Kex2p cytosolic tail. SOI3, identical to RAV1, encodes a protein important for regulated assembly of vacuolar ATPase. Here, we show that Soi3/Rav1p is required for transport between the early endosome and the late endosome/prevacuolar compartment (PVC). By electron microscopy, soi3-1 mutants massively accumulated structures that resembled early endosomes. soi3Delta mutants exhibited a kinetic delay in transfer of the endocytic tracer dye FM4-64, from the 14 degrees C endocytic intermediate to the vacuole. The soi3Delta mutation delayed vacuolar degradation but not internalization of the a-factor receptor Ste3p. By density gradient fractionation, Soi3/Rav1p associated as a peripheral protein with membranes of a density characteristic of early endosomes. The soi3 null mutation markedly reduced the rate of Kex2p transport from the TGN to the PVC but had no effect on vacuolar protein sorting or cycling of Vps10p. These results suggest that assembly of vacuolar ATPase at the early endosome is required for transport of both Ste3p and Kex2p from the early endosome to the PVC and support a model in which cycling through the early endosome is part of the normal itinerary of Kex2p and other TGN-resident proteins.
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Vesículas Citoplasmáticas/fisiologia , Endocitose/fisiologia , Proteínas de Membrana/metabolismo , Proteínas de Membrana/fisiologia , Proteínas de Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/fisiologia , Saccharomyces cerevisiae/fisiologia , Rede trans-Golgi/fisiologia , Proteínas de Transporte de Cátions/análise , Proteínas de Transporte de Cátions/metabolismo , Endocitose/genética , Endossomos/fisiologia , Proteínas de Ligação ao GTP/metabolismo , Proteínas de Membrana/análise , Proteínas de Membrana/genética , Mutação/genética , Pró-Proteína Convertases/genética , Pró-Proteína Convertases/metabolismo , Transporte Proteico/genética , Transporte Proteico/fisiologia , Receptores Acoplados a Proteínas G/análise , Receptores Acoplados a Proteínas G/metabolismo , Receptores de Fator de Acasalamento , Receptores de Feromônios/análise , Receptores de Feromônios/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/imunologia , Proteínas de Saccharomyces cerevisiae/análise , Proteínas de Saccharomyces cerevisiae/genética , Deleção de Sequência/genética , Vacúolos/imunologia , Vacúolos/fisiologia , Vacúolos/ultraestrutura , Proteínas de Transporte Vesicular/análise , Proteínas de Transporte Vesicular/fisiologiaRESUMO
Armillaria species are both devastating forest pathogens and some of the largest terrestrial organisms on Earth. They forage for hosts and achieve immense colony sizes via rhizomorphs, root-like multicellular structures of clonal dispersal. Here, we sequenced and analysed the genomes of four Armillaria species and performed RNA sequencing and quantitative proteomic analysis on the invasive and reproductive developmental stages of A. ostoyae. Comparison with 22 related fungi revealed a significant genome expansion in Armillaria, affecting several pathogenicity-related genes, lignocellulose-degrading enzymes and lineage-specific genes expressed during rhizomorph development. Rhizomorphs express an evolutionarily young transcriptome that shares features with the transcriptomes of both fruiting bodies and vegetative mycelia. Several genes show concomitant upregulation in rhizomorphs and fruiting bodies and share cis-regulatory signatures in their promoters, providing genetic and regulatory insights into complex multicellularity in fungi. Our results suggest that the evolution of the unique dispersal and pathogenicity mechanisms of Armillaria might have drawn upon ancestral genetic toolkits for wood-decay, morphogenesis and complex multicellularity.
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Armillaria/genética , Proteínas Fúngicas/genética , Genoma Fúngico , Proteômica , Análise de Sequência de RNA , Especificidade da Espécie , TranscriptomaRESUMO
Pleiotropic drug resistance (PDR) is a well-described phenomenon occurring in fungi. PDR shares several similarities with processes in bacteria and higher eukaryotes. In mammalian cells, multidrug resistance (MDR) develops from an initial single drug resistance, eventually leading to a broad cross-resistance to many structurally and functionally unrelated compounds. Notably, a number of membrane-embedded energy-consuming ATP-binding cassette (ABC) transporters have been implicated in the development of PDR/MDR phenotypes. The yeast Saccharomyces cerevisiae genome harbors some 30 genes encoding ABC proteins, several of which mediate PDR. Therefore, yeast served as an important model organism to study the functions of evolutionary conserved ABC genes, including those mediating clinical antifungal resistance in fungal pathogens. Moreover, yeast cells lacking endogenous ABC pumps are hypersensitive to many antifungal drugs, making them suitable for functional studies and cloning of ABC transporters from fungal pathogens such as Candida albicans. This review discusses drug resistance phenomena mediated by ABC transporters in the model system S. cerevisiae and certain fungal pathogens.
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Transportadores de Cassetes de Ligação de ATP/metabolismo , Farmacorresistência Fúngica/genética , Fungos/efeitos dos fármacos , Fungos/metabolismo , Animais , Fungos/genética , Humanos , Micoses/tratamento farmacológico , Micoses/microbiologia , Fenótipo , Especificidade por SubstratoRESUMO
Endogenous cytokinins were quantified in synchronized Chlorella minutissima Fott et Novákova (MACC 361) and Chlorella sp. (MACC 458) grown in a 14:10 light:dark (L:D) photoperiod. In 24 h experiments, cell division occurred during the dark period, and cells increased in size during the light period. Cytokinin profiles were similar in both strains, consisting of five cis-zeatin (cZ) and three N(6) -(2-isopentenyl)adenine (iP) derivatives. Cytokinin concentrations were low during the dark period and increased during the light period. In 48 h experiments using synchronized C. minutissima (MACC 361), half the cultures were maintained in continuous dark conditions for the second photoperiod. Cell division occurred during both dark periods, and cells increased in size during the light periods. Cultures kept in continuous dark did not increase in size following cell division. DNA analysis confirmed these results, with cultures grown in light having increased DNA concentrations prior to cell division, while cultures maintained in continuous dark had less DNA. Cytokinins (cZ and iP derivatives) were detected in all samples with concentrations increasing over the first 24 h. This increase was followed by a large increase, especially during the second light period where cytokinin concentrations increased 4-fold. Cytokinin concentrations did not increase in cultures maintained in continuous dark conditions. In vivo deuterium-labeling technology was used to measure cytokinin biosynthetic rates during the dark and light periods in C. minutissima with highest biosynthetic rates measured during the light period. These results show that there is a relationship between light, cell division, and cytokinins.