Dynamic multifunctional devices enabled by ultrathin metal nanocoatings with optical/photothermal and morphological versatility.

Inspired by the intriguing adaptivity of natural life, such as squids and flowers, we propose a series of dynamic and responsive multifunctional devices based on multiscale structural design, which contain metal nanocoating layers overlaid with other micro-/nanoscale soft or rigid layers. Since the optical/photothermal properties of a metal nanocoating are thickness dependent, metal nanocoatings with different thicknesses were chosen to integrate with other structural design elements to achieve dynamic multistimuli responses. The resultant devices demonstrate 1) strain-regulated cracked and/or wrinkled topography with tunable light-scattering properties, 2) moisture/photothermal-responsive structural color coupled with wrinkled surface, and 3) mechanically controllable light-shielding properties attributed to the strain-dependent crack width of the nanocoating. These devices can adapt external stimuli, such as mechanical strain, moisture, light, and/or heat, into corresponding changes of optical signals, such as transparency, reflectance, and/or coloration. Therefore, these devices can be applied as multistimuli-responsive encryption devices, smart windows, moisture/photothermal-responsive dynamic optics, and smartphone app-assisted pressure-mapping sensors. All the devices exhibit high reversibility and rapid responsiveness. Thus, this hybrid system containing ultrathin metal nanocoatings holds a unique design flexibility and adaptivity and is promising for developing next-generation multifunctional devices with widespread application.

Global patterns of illegal marine turtle exploitation.

Human exploitation of wildlife for food, medicine, curios, aphrodisiacs, and spiritual artifacts represents a mounting 21st-century conservation challenge. Here, we provide the first global assessment of illegal marine turtle exploitation across multiple spatial scales (i.e., Regional Management Units [RMUs] and countries) by collating data from peer-reviewed studies, grey literature, archived media reports, and online questionnaires of in-country experts spanning the past three decades. Based on available information, we estimate that over 1.1 million marine turtles were exploited between 1990 and 2020 against existing laws prohibiting their use in 65 countries or territories and in 44 of the world's 58 marine turtle RMUs, with over 44,000 turtles exploited annually over the past decade. Exploitation across the 30-year period primarily consisted of green (56%) and hawksbill (39%) turtles when identified by species, with hawksbills (67%) and greens (81%) comprising the majority of turtles exploited in the 1990s and 2000s, respectively, and both species accounting for similar levels of exploitation in the 2010s. Although there were no clear overarching trends in the magnitude or spatial patterns of exploitation across the three decades, there was a 28% decrease in reported exploitation from the 2000s to the 2010s. The 10 RMUs with the highest exploitation in the 2010s included seven green and three hawksbill turtle RMUs, with most reported exploitation occurring in RMUs that typically exhibit a low risk of population decline or loss of genetic diversity. Over the past decade, the number of RMUs with "moderate" or "high" exploitation impact scores decreased. Our assessment suggests that illegal exploitation appears to have declined over the past decade and, with some exceptions, is primarily occurring in large, stable, and genetically diverse marine turtle populations.

Super Stretchable and Compressible Hydrogels Inspired by Hook-and-Loop Fasteners.

Inspired by hook-and-loop fasteners, we designed a hydrogel network containing α-zirconium phosphate (ZrP) two-dimensional nanosheets with a high density of surface hydroxyl groups serving as nanopatches with numerous "hooks," while polymer chains with plentiful amine functional groups serve as "loops." Our multiscale molecular simulations confirm that both the high density of hydroxyl groups on nanosheets and the large number of amine functional groups on polymer chains are essential to achieve reversible interactions at the molecular scale, functioning as nano hook-and-loop fasteners to dissipate energy. As a result, the synthesized hydrogel possesses superior stretchability (>2100% strain), resilience to compression (>90% strain), and durability. Remarkably, the hydrogel can sustain >5000 cycles of compression with torsion in a solution mimicking synovial fluid, thus promising for potential biomedical applications such as artificial articular cartilage. This hook-and-loop model can be adopted and generalized to design a wide range of multifunctional materials with exceptional mechanical properties.

Bioinformatic prospecting and phylogenetic analysis reveals 94 undescribed circular bacteriocins and key motifs.

BACKGROUND: Circular bacteriocins are antimicrobial peptides produced by bacteria with a N and C termini ligation. They have desirable properties such as activity at low concentrations along with thermal, pH and proteolytic resistance. There are twenty experimentally confirmed circular bacteriocins as part of bacteriocin gene clusters, with transport, membrane and immunity proteins. Traditionally, novel antimicrobials are found by testing large numbers of isolates against indicator strains, with no promise of corresponding novel sequence. RESULTS: Through bioprospecting publicly available sequence databases, we identified ninety-nine circular bacteriocins across a variety of bacteria bringing the total to 119. They were grouped into two families within class I modified bacteriocins (i and ii) and further divided into subfamilies based on similarity to experimentally confirmed circular bacteriocins. Within subfamilies, sequences overwhelmingly shared similar characteristics such as sequence length, presence of a polybasic region, conserved locations of aromatic residues, C and N termini, gene clusters similarity, translational coupling and hydrophobicity profiles. At least ninety were predicted to be putatively functional based on gene clusters. Furthermore, bacteriocins identified from Enterococcus, Staphylococcus and Streptococcus species may have activity against clinically relevant strains, due to the presence of putative immunity genes required for expression in a toxin-antitoxin system. Some strains such as Paenibacillus larvae subsp. pulvifaciens SAG 10367 contained multiple circular bacteriocin gene clusters from different subfamilies, while some strains such as Bacillus cereus BCE-01 contained clusters with multiple circular bacteriocin structural genes. CONCLUSIONS: Sequence analysis provided rapid insight into identification of novel, putative circular bacteriocins, as well as conserved genes likely essential for circularisation. This represents an expanded library of putative antimicrobial proteins which are potentially active against human, plant and animal pathogens.

Connectivity of the Cingulate Sulcus Visual Area (CSv) in the Human Cerebral Cortex.

The human cingulate sulcus visual area (CSv) responds selectively to visual and vestibular cues to self-motion. Although it is more selective for visual self-motion cues than any other brain region studied, it is not known whether CSv mediates perception of self-motion. An alternative hypothesis, based on its location, is that it provides sensory information to the motor system for use in guiding locomotion. To evaluate this hypothesis we studied the connectivity pattern of CSv, which is completely unknown, with a combination of diffusion MRI and resting-state functional MRI. Converging results from the 2 approaches suggest that visual drive is provided primarily by areas hV6, pVIP (putative intraparietal cortex) and PIC (posterior insular cortex). A strong connection with the medial portion of the somatosensory cortex, which represents the legs and feet, suggests that CSv may receive locomotion-relevant proprioceptive information as well as visual and vestibular signals. However, the dominant connections of CSv are with specific components of the motor system, in particular the cingulate motor areas and the supplementary motor area. We propose that CSv may provide a previously unknown link between perception and action that serves the online control of locomotion.

The role of spatial structure in the collapse of regional metapopulations.

Many wildlife populations are either naturally, or as a result of human land use, patchily distributed in space. The degree of fragmentation-specifically the remaining patch sizes and habitat configuration-is an important part of population dynamics. Demographic stochasticity is also likely to play an important role in patchy habitats that host small local populations. We develop a simulation model to evaluate the significance of demographic stochasticity and the role fragmentation plays in the determination of population dynamics and the risk of extinction of populations on habitat patches. Our model is formulated as a Markov-chain stochastic process on a finite, spatially explicit array of patches in which probability of successful dispersal is a function of interpatch distance. Unlike past work, we explicitly model local population dynamics and examine how these scale up to the entire population. As a test case, we apply the model to the American pika (Ochotona princeps) population living on the ore dumps in the ghost mining town of Bodie, California. This population has been studied nearly continuously for over four decades and has been of conservation concern as the southern half of the population declined precipitously beginning in 1989. Our model suggests that both the specific configuration of habitat and landscape heterogeneity are necessary and sufficient predictors of the eventual extinction of the southern constellation of patches. This example has important implications, as it suggests that fragmentation alone can lead to regional extinctions within metapopulations.

Processing of Egomotion-Consistent Optic Flow in the Rhesus Macaque Cortex.

The cortical network that processes visual cues to self-motion was characterized with functional magnetic resonance imaging in 3 awake behaving macaques. The experimental protocol was similar to previous human studies in which the responses to a single large optic flow patch were contrasted with responses to an array of 9 similar flow patches. This distinguishes cortical regions where neurons respond to flow in their receptive fields regardless of surrounding motion from those that are sensitive to whether the overall image arises from self-motion. In all 3 animals, significant selectivity for egomotion-consistent flow was found in several areas previously associated with optic flow processing, and notably dorsal middle superior temporal area, ventral intra-parietal area, and VPS. It was also seen in areas 7a (Opt), STPm, FEFsem, FEFsac and in a region of the cingulate sulcus that may be homologous with human area CSv. Selectivity for egomotion-compatible flow was never total but was particularly strong in VPS and putative macaque CSv. Direct comparison of results with the equivalent human studies reveals several commonalities but also some differences.

Versatile Nanostructures from Rice Husk Biomass for Energy Applications.

Converting biomass into valuable products has great benefits in terms of both economic and environmental considerations, and has attracted considerable attention in recent years. Rice husk biomass was initially utilized to produce bulk materials for conventional applications while a variety of advanced nanostructures (NSs) have been fabricated over the past few years. In addition to their low cost and environmental friendliness, RH-derived NSs (RH-NSs) exhibit versatile properties, which are promising for broad applications in various fields. In this Review, we summarize the latest research on RH-NSs, covering their design, fabrication, properties, and applications in the modern energy field. Based on the unique structure and components of RHs, a series of carbon/silicon-based novel NSs with outstanding performances have been exploited, which are difficult to be synthesized using conventional chemical reagents. We also discuss perspective uses of RH-NSs on the basis of the current research progress.

Global Motion Processing in Human Visual Cortical Areas V2 and V3.

UNLABELLED: Global motion perception entails the ability to extract the central direction tendency from an extended area of visual space containing widely disparate local directions. A substantial body of evidence suggests that local motion signals generated in primary visual cortex (V1) are spatially integrated to provide perception of global motion, beginning in the middle temporal area (MT) in macaques and its counterpart in humans, hMT. However, V2 and V3 also contain motion-sensitive neurons that have larger receptive fields than those found in V1, giving the potential for spatial integration of motion signals. Despite this, V2 and V3 have been overlooked as sites of global motion processing. To test, free of local-global confounds, whether human V2 and V3 are important for encoding global motion, we developed a visual stimulus that yields a global direction yet includes all possible local directions and is perfectly balanced at the local motion level. We then attempted to decode global motion direction in such stimuli with multivariate pattern classification of fMRI data. We found strong sensitivity to global motion in hMT, as expected, and also in several higher visual areas known to encode optic flow. Crucially, we found that global motion direction could be decoded in human V2 and, particularly, in V3. The results suggest the surprising conclusion that global motion processing is a key function of cortical visual areas V2 and V3. A possible purpose is to provide global motion signals to V6. SIGNIFICANCE STATEMENT: Humans can readily detect the overall direction of movement in a flock of birds despite large differences in the directions of individual birds at a given moment. This ability to combine disparate motion signals across space underlies many aspects of visual motion perception and has therefore received considerable research attention. The received wisdom is that spatial integration of motion signals occurs in the cortical motion complex MT+ in both human and nonhuman primates. We show here that areas V2 and V3 in humans are also able to perform this function. We suggest that different cortical areas integrate motion signals in different ways for different purposes.

Neural mechanisms for discounting head-roll-induced retinal motion.

An extensive series of physiological studies in macaques shows the existence of neurons in three multisensory cortical regions, dorsal medial superior temporal area (MSTd), ventral intraparietal area (VIP), and visual posterior sylvian area (VPS), that are tuned for direction of self-motion in both visual and vestibular modalities. Some neurons have congruent direction preferences, suggesting integration of signals for optimum encoding of self-motion trajectory; others have opposite preferences and could be used for discounting retinal motion that arises from perceptually irrelevant head motion. Whether such a system exists in humans is unknown. Here, artificial vestibular stimulation was administered to human participants during fMRI scanning in conjunction with carefully calibrated visual stimulation that emulated either congruent or opposite stimulation conditions. Direction and speed varied sinusoidally, such that the two conditions contained identical vestibular stimulation and identical retinal stimulation, differing only in the relative phase of the two components. In human MST and putative VIP, multivoxel pattern analysis permitted classification of stimulus phase based on fMRI time-series data, consistent with the existence of separate neuron populations responsive to congruent and opposite cue combinations. Decoding was also possible in the vicinity of parieto-insular vestibular cortex, possibly in a homolog of macaque VPS.

Macrolactam analogues of macrolide natural products.

The chemical modification of macrolide natural products into aza- or lactam analogues is a strategy employed to improve their metabolic stability and biological activity. The methods for the synthesis of several lactam analogues of macrolide natural products are highlighted and aspects of their biological properties presented.

LaGomiCs-Lagomorph Genomics Consortium: An International Collaborative Effort for Sequencing the Genomes of an Entire Mammalian Order.

The order Lagomorpha comprises about 90 living species, divided in 2 families: the pikas (Family Ochotonidae), and the rabbits, hares, and jackrabbits (Family Leporidae). Lagomorphs are important economically and scientifically as major human food resources, valued game species, pests of agricultural significance, model laboratory animals, and key elements in food webs. A quarter of the lagomorph species are listed as threatened. They are native to all continents except Antarctica, and occur up to 5000 m above sea level, from the equator to the Arctic, spanning a wide range of environmental conditions. The order has notable taxonomic problems presenting significant difficulties for defining a species due to broad phenotypic variation, overlap of morphological characteristics, and relatively recent speciation events. At present, only the genomes of 2 species, the European rabbit (Oryctolagus cuniculus) and American pika (Ochotona princeps) have been sequenced and assembled. Starting from a paucity of genome information, the main scientific aim of the Lagomorph Genomics Consortium (LaGomiCs), born from a cooperative initiative of the European COST Action "A Collaborative European Network on Rabbit Genome Biology-RGB-Net" and the World Lagomorph Society (WLS), is to provide an international framework for the sequencing of the genome of all extant and selected extinct lagomorphs. Sequencing the genomes of an entire order will provide a large amount of information to address biological problems not only related to lagomorphs but also to all mammals. We present current and planned sequencing programs and outline the final objective of LaGomiCs possible through broad international collaboration.

Activity in the human superior colliculus relating to endogenous saccade preparation and execution.

In recent years a small number of studies have applied functional imaging techniques to investigate visual responses in the human superior colliculus (SC), but few have investigated its oculomotor functions. Here, in two experiments, we examined activity associated with endogenous saccade preparation. We used 3-T fMRI to record the hemodynamic activity in the SC while participants were either preparing or executing saccadic eye movements. Our results showed that not only executing a saccade (as previously shown) but also preparing a saccade produced an increase in the SC hemodynamic activity. The saccade-related activity was observed in the contralateral and to a lesser extent the ipsilateral SC. A second experiment further examined the contralateral mapping of saccade-related activity with a larger range of saccade amplitudes. Increased activity was again observed in both the contralateral and ipsilateral SC that was evident for large as well as small saccades. This suggests that the ipsilateral component of the increase in BOLD is not due simply to small-amplitude saccades producing bilateral activity in the foveal fixation zone. These studies provide the first evidence of presaccadic preparatory activity in the human SC and reveal that fMRI can detect activity consistent with that of buildup neurons found in the deeper layers of the SC in studies of nonhuman primates.

Flow sensing in developing Xenopus laevis is disrupted by visual cues and ototoxin exposure.

We explored how lateral line cues interact with visual cues to mediate flow sensing behaviors in the nocturnal developing frog, Xenopus laevis, by exposing animals to current flows under different lighting conditions and after exposure to the ototoxin gentamicin. Under dark conditions, Xenopus tadpoles move downstream at the onset of current flow, then turn, and orient toward the direction of the flow with high accuracy. Postmetamorphic froglets also exhibit positive rheotaxis but with less accuracy and longer latency. The addition of discrete light cues to an otherwise dark environment disrupts rheotaxis and positioning. Orientation is less accurate, latency to orient is longer, and animals do not move as far downstream in the presence of light. Compared with untreated tadpoles tested in the dark, tadpoles exposed to gentamicin show less accurate rheotaxis with longer latency and do not move as far downstream in response to flow. These effects are compounded by the presence of light cues. The disruptive effects of light on flow sensing in Xenopus emphasize the disturbances to natural behaviors that may be produced by anthropogenic illumination in nocturnal habitats.

A representation of changing heading direction in human cortical areas pVIP and CSv.

When we move around in the environment, we continually change direction. Much work has examined how the brain extracts instantaneous direction of heading from optic flow but how changes in heading are encoded is unknown. Change could simply be inferred cognitively from successive instantaneous heading values, but we hypothesize that heading change is represented as a low-level signal that feeds into motor control with minimal need for attention or cognition. To test this, we first used functional MRI to measure activity in several predefined visual areas previously associated with processing optic flow (hMST, hV6, pVIP, and CSv) while participants viewed flow that simulated either constant heading or changing heading. We then trained a support vector machine (SVM) to distinguish the multivoxel activity pattern elicited by rightward versus leftward changes in heading direction. Some motion-sensitive visual cortical areas, including hMST, responded well to flow but did not appear to encode heading change. However, visual areas pVIP and, particularly, CSv responded with strong selectivity to changing flow and also allowed direction of heading change to be decoded. This suggests that these areas may construct a representation of heading change from instantaneous heading directions, permitting rapid and accurate preattentive detection and response to change.

The pika and the watershed: The impact of small mammal poisoning on the ecohydrology of the Qinghai-Tibetan Plateau.

With approximately 20 % of the world's population living in its downstream watersheds, the Qinghai-Tibetan Plateau (QTP) is considered "Asia's Water Tower." However, grasslands of the QTP, where most of Asia's great rivers originate, are becoming increasingly degraded, which leads to elevated population densities of a native small mammal, the plateau pika (Ochotona curzoniae). As a result pikas have been characterized as a pest leading to wide-spread poisoning campaigns in an attempt to restore grassland quality. A contrary view is that pikas are a keystone species for biodiversity and that their burrowing activity provides a critical ecosystem service by increasing the infiltration rate of water, hence reducing overland flow. We demonstrate that poisoning plateau pikas significantly reduces infiltration rate of water across the QTP creating the potential for watershed-level impacts. Our results demonstrate the importance of burrowing mammals as ecosystem engineers, particularly with regard to their influence on hydrological functioning.

White matter tracts adjacent to the human cingulate sulcus visual area (CSv).

Human cingulate sulcus visual area (CSv) was first identified as an area that responds selectively to visual stimulation indicative of self-motion. It was later shown that the area is also sensitive to vestibular stimulation as well as to bodily motion compatible with locomotion. Understanding the anatomical connections of CSv will shed light on how CSv interacts with other parts of the brain to perform information processing related to self-motion and navigation. A previous neuroimaging study (Smith et al. 2018, Cerebral Cortex, 28, 3685-3596) used diffusion-weighted magnetic resonance imaging (dMRI) to examine the structural connectivity of CSv, and demonstrated connections between CSv and the motor and sensorimotor areas in the anterior and posterior cingulate sulcus. The present study aimed to complement this work by investigating the relationship between CSv and adjacent major white matter tracts, and to map CSv's structural connectivity onto known white matter tracts. By re-analysing the dataset from Smith et al. (2018), we identified bundles of fibres (i.e. streamlines) from the whole-brain tractography that terminate near CSv. We then assessed to which white matter tracts those streamlines may belong based on previously established anatomical prescriptions. We found that a significant number of CSv streamlines can be categorised as part of the dorsalmost branch of the superior longitudinal fasciculus (SLF I) and the cingulum. Given current thinking about the functions of these white matter tracts, our results support the proposition that CSv provides an interface between sensory and motor systems in the context of self-motion.

Evaluating the Radiation Sensitivity Index and 12-chemokine gene expression signature for clinical use in a CLIA laboratory.

Background: The radiation sensitivity index (RSI) and 12-chemokine gene expression signature (12CK GES) are two gene expression signatures (GES) that were previously developed to predict tumor radiation sensitivity or identify the presence of tertiary lymphoid structures in tumors, respectively. To advance the use of these GES into clinical trial evaluation, their assays must be assessed within the context of the Clinical Laboratory Improvement Amendments (CLIA) process. Methods: Using HG-U133Plus 2.0 arrays, we first established CLIA laboratory proficiency. Then the accuracy (limit of detection and macrodissection impact), precision (variability by time and operator), sample type (surgery vs. biopsy), and concordance with reference laboratory were evaluated. Results: RSI and 12CK GES were reproducible (RSI: 0.01 mean difference, 12CK GES 0.17 mean difference) and precise with respect to time and operator. Taken together, the reproducibility analysis of the scores indicated a median RSI difference of 0.06 (6.47% of range) across samples and a median 12CK GES difference of 0.92 (12.29% of range). Experiments indicated that the lower limit of input RNA is 5 ng. Reproducibility with a second CLIA laboratory demonstrated reliability with the median RSI score difference of 0.065 (6% of full range) and 12CK GES difference of 0.93 (12 % of observed range). Conclusions: Overall, under CLIA, RSI and 12CK GES were demonstrated by the Moffitt Cancer Center Advanced Diagnostic Laboratory to be reproducible GES for clinical usage.

Implicit representations of luminance and the temporal structure of moving stimuli in multiple regions of human visual cortex revealed by multivariate pattern classification analysis.

The generation of a behaviorally relevant cue to the speed of objects around us is critical to our ability to navigate safely within our environment. However, our perception of speed is often distorted by prevailing conditions. For instance, as luminance is reduced, our perception of the speed of fast-moving patterns can be increased by as much as 30%. To investigate how the cortical representation of speed may vary under such conditions, we have measured the functional MRI blood oxygen level-dependent (BOLD) response of visual cortex to drifting sine gratings at two very different luminances. The average BOLD response in all areas was band-pass with respect to speed (or equivalently, temporal frequency) and thus contained no unambiguous speed information. However, a multivariate classifier was able to predict grating speed successfully in all cortical areas measured. Similarly, we find that a multivariate classifier can predict stimulus luminance. No differences in either the mean BOLD response or the multivariate classifier response with respect to speed were found as luminance changed. However, examination of the spatial distribution of speed preferences in the primary visual cortex revealed that perifoveal locations preferred slower speeds than peripheral locations at low but not high luminance. We conclude that although an explicit representation of perceived speed has yet to be demonstrated in the human brain, multiple visual regions encode both the temporal structure of moving stimuli and luminance implicitly.