Schistosomiasis in migrant children and adolescents in a paediatric tropical referral unit in Spain: diagnosis and long-term management challenges.

Globalisation and population movement have led to an increasing number of migrant children residing in areas non-endemic for schistosomiasis. However, diagnosing and managing schistosomiasis in children remain controversial. This study aims to investigate the prevalence of schistosomiasis in migrant children and to describe the diagnostic approach and management strategies, including long-term follow-up, to explore the potential role of serological tests in evaluating treatment response. We conducted a retrospective descriptive study spanning from January 2014-July 2021 at a referral unit for Paediatric Tropical Diseases in Madrid (Spain). The study included patients under 18 years diagnosed with schistosomiasis. Of 679 children screened for schistosomiasis, 73 (10.8%) tested positive. The median age was 16.3 years [IQR 9-17.6], 74% male. The majority originated from Sub-Saharan Africa (47%) and Asia (47%). Only 40% presented with symptoms, with gastrointestinal (18%) and cutaneous (17%) manifestations being the most common. Eosinophilia was observed in 43% (median [IQR]: 1103/mm3 [671-1536]), and ova were visualised in the urine of 2/50 (4.0%). Praziquantel treatment was administered to 92%, and 5 patients required retreatment. Follow-up data were available for 58 (80%) over a median period of 9 months [IQR 6-19.8], revealing a progressive decline in eosinophil count, IgE titres, and ELISA optical density.    Conclusion: In this series, the prevalence of schistosomiasis among migrant children was significant (10%), highlighting the importance of including serological tests in migrant health screening. The disease is largely asymptomatic, eosinophilia is often absent, and visualisation of ova in urine is exceedingly rare. Eosinophil count, IgE titres, and ELISA optical density could prove valuable as an initial approach for monitoring inflammation during follow-up assessments. What is Known: • The burden of disease related to schistosomiasis is significant, particulary in children, and it is advisable to screen this vulnerable population. What is New: • Eosinophilia may not be present in parasitic infections, so serological tests are crucial for screening migrant children. • Serological monitoring facilitates long-term management of migrant children with schistosomiasis.

Synergism between remdesivir and ribavirin leads to SARS-CoV-2 extinction in cell culture.

BACKGROUND AND PURPOSE: There is a need for effective anti-COVID-19 treatments, mainly for individuals at risk of severe disease such as the elderly and the immunosuppressed. Drug repositioning has proved effective in identifying drugs that can find a new application for the control of coronavirus disease, in particular COVID-19. The purpose of the present study was to find synergistic antiviral combinations for COVID-19 based on lethal mutagenesis. EXPERIMENTAL APPROACH: The effect of combinations of remdesivir and ribavirin on the infectivity of SARS-CoV-2 in cell culture has been tested. Viral populations were monitored by ultra-deep sequencing, and the decrease of infectivity as a result of the treatment was measured. KEY RESULTS: Remdesivir and ribavirin exerted a synergistic inhibitory activity against SARS-CoV-2, quantified both by CompuSyn (Chou-Talalay method) and Synergy Finder (ZIP-score model). In serial passage experiments, virus extinction was readily achieved with remdesivir-ribavirin combinations at concentrations well below their cytotoxic 50 value, but not with the drugs used individually. Deep sequencing of treated viral populations showed that remdesivir, ribavirin, and their combinations evoked significant increases of the number of viral mutations and haplotypes, as well as modification of diversity indices that characterize viral quasi-species. CONCLUSION AND IMPLICATIONS: SARS-CoV-2 extinction can be achieved by synergistic combination treatments based on lethal mutagenesis. In addition, the results offer prospects of triple drug treatments for effective SARS-CoV-2 suppression.

CENTRAL NERVOUS SYSTEM INFECTION BY GROUP A STREPTOCOCCUS IN CHILDREN IN SPAIN (2019-2023).

Central nervous system infections in children caused by group A Streptococcus are rare. This study, conducted across 52 hospitals in Spain from 2019 to 2023, identified 32 cases of central nervous system infections in children caused by group A Streptococcus, with a significant increase from October 2022 onward (1.1% vs. 5.9%, P = 0.002). Half required pediatric intensive care unit admission, 12.5% exhibited sequelae and the mortality rate was 6.2%. Mastoiditis was the predominant primary infection.

Efecto de intervenciones de psicología positiva y ejercicio físico en el bienestar y malestar psicológico de estudiantes universitarios / Effect of positive psychology interventions and physical exercise on the well-being and psychological distress of university students

Resumen El propósito de este estudio fue analizar el efecto de intervenciones de Psicología positiva y de ejercicio físico sobre indicadores de bienestar y malestar psicológico en estudiantes universitarios costarricenses. Se realizó una investigación cuasiexperimental, de diseño factorial (4 x 2) con mediciones pre y post, en el que 233 estudiantes universitarios (18.97 ± 2.18 años, 135 mujeres, 98 hombres) se vieron expuestos a una de cuatro diferentes condiciones: intervenciones de psicología positiva (PP), intervenciones de ejercicio físico (E), intervenciones combinadas de PP y E y control (C). Se midieron indicadores de bienestar y malestar psicológico. Las intervenciones de Psicología positiva mejoraron el indicador de malestar psicológico "emociones negativas" (p = .022), pero no impactaron significativamente los indicadores de bienestar psicológico (p > .05). La combinación las intervenciones de PP y E generó los mismos efectos que cuando únicamente se utilizan las estrategias derivadas de la psicología positiva, esto es, se presentó una reducción significativa en el indicador "emociones negativas" (p = .028). Dicho efecto puede contribuir a la mejora de la vivencia de la vida universitaria y proteger a los estudiantes de los riesgos asociados con experiencias de malestar psicológico en diversas esferas de su vida. Las intervenciones de ejercicio por sí mismas no modificaron de manera significativa los indicadores de bienestar ni malestar psicológico (p > .05).

Abstract: The purpose of this study was to analyze the effect of positive psychology and exercise interventions on psychological well-being and ill-being indicators in Costa Rican college students. This study had a quasi-experimental factorial (4 x 2) design, 233 college students (18.97 ± 2.18 years, 135 females, 98 males) were assigned to one of four different conditions: positive psychological interventions (PP), exercise interventions (E), combined (PP + E) interventions, and control group (C). Psychological well-being and ill-being indicators were measured pre and post interventions. Positive psychological interventions improved the "negative emotions" indicator (p = .022); but did not have a significant impact on well-being indicators (p > .05). The combination of PP and E interventions produced the same effects as PP alone; a decrease in "negative emotions" was observed (p = .028). This effect might contribute to improve the experience of university life for higher education students and protect them from the risks associated with psychological ill-being. Exercise interventions alone did not change either the psychological well-being or ill-being indicators (p > .05).