Carbon nanotube enhanced membrane distillation for salty and dyeing wastewater treatment by electrospinning technology.

Membrane distillation (MD) is considered as a promising and attractive technology due to its effective production of fresh water. However, the low permeability and easy wetting of MD membranes limit its practical applications. Herein carbon nanotubes (CNTs) and polyvinylidene fluoride-co-hexafluoropropylene (PcH) were used to fabricate nanofiber membranes by electrospinning. Effects of heat-press temperature and CNTs concentration on the morphology and performance of the as-fabricated membranes were systematically investigated. Dye rejections of CNTs/PcH membranes were also studied and role of CNTs played in the as-prepared MD membranes were analyzed. Results suggest that heat-press treatment effectively improved the mechanical strength as well as liquid entry pressure of membranes, and the optimal heat-press temperature was 150 °C. CNTs were proved to be successfully blended in nanofibers. Hydrophobicity and mechanical strength of membranes increased with CNTs incorporation. The 0.5 wt % CNTs loaded membrane heat-pressed at 150 °C exhibited the highest permeate flux (16.5-18.5 L m-2 h-1), which signified an increase of 42-50 % compared to the commercial MD membrane (11-13 L m-2 h-1) when 35 and 70 g L-1 NaCl solutions were used as feed solutions, respectively. It was noteworthy that salt rejection efficiencies of tested membranes achieved more than 99.99 %. When CNTs/PcH nanofiber membrane was applied to the treatment of dyeing wastewater, the removal rates of acid red and acid yellow reached 100 %. The removal rates of methylene blue and crystal violet were 99.41 % and 99.91 %, respectively. The present study suggested that the as-prepared membranes showed high potential towards MD application.

Two new ent-kaurane diterpenes from the stems of Eurya chinensis.

Two new ent-kaurane diterpenes (1-2), together with five known analogs, were isolated from the stems of Eurya chinensis. The structures of new compounds were established by extensive analysis of mass spectrometric and 1D and 2D NMR spectroscopic data. Compound 3 exhibited noticeable anti-inflammatory activity as denoted by inhibiting LPS-induced nitric oxide (NO) production in RAW264.7 cells with an IC50 value of 7.82 µM. Compound 4 showed potent cytotoxic activity against human cancer cell lines NCI-H46, HepG2 and SW480 with IC50 values ranging from 7.45 to 8.54 µM.

Anti-inflammatory and antimicrobial coumarins from the stems of Eurya chinensis.

Two new coumarins, named (±)-euryacoumarin A (1) and 6-demethylobtusinin (2), and one new natural coumarin, named euryacoumarin B (3), along with two known compounds, scopoletin (4) and obtusinol (5), were isolated from the stems of Eurya chinensis. Their structures were elucidated by means of extensive spectroscopic methods and comparison with data reported in the literatures. Compound 1 exhibited significant inhibition of LPS-induced nitric oxide (NO) production in RAW264.7 cells with IC50 value of 35.64 ± 1.73 µM, and showed marginal antibacterial activities against Bacillus subtilis and B. cereus with MIC values of 50.59 ± 2.12 and 35.42 ± 0.96 µM, respectively.

[Chemical Constituents from Mitrasacme pygmaea].

Objective: To study the chemical constituents from Mitrasacme pygmaea. Methods: The compounds were isolated and purified by column chromatography and their structure were identified by NMR and MS,and comparison spectral data with literature. Results: Eleven compounds were isolated and identified as tricin-7-O-ß-D-glucopyranoside( 1),massonianoid A( 2),kaempferol( 3),cinnamic acid( 4),quercetin( 5),tiliroside( 6),tricin( 7),ß-sitosterol( 8),adenosine( 9),α-tocopherolquinone( 10)and ß-daucosterol( 11). Conclusion: All the compounds are isolated from this genus for the first time.

Mechanistic prediction and validation of Brevilin A Therapeutic effects in Lung Cancer.

BACKGROUND: Traditional Chinese medicine (TCM) has been found widespread application in neoplasm treatment, yielding promising therapeutic candidates. Previous studies have revealed the anti-cancer properties of Brevilin A, a naturally occurring sesquiterpene lactone derived from Centipeda minima (L.) A.Br. (C. minima), a TCM herb, specifically against lung cancer. However, the underlying mechanisms of its effects remain elusive. This study employs network pharmacology and experimental analyses to unravel the molecular mechanisms of Brevilin A in lung cancer. METHODS: The Batman-TCM, Swiss Target Prediction, Pharmmapper, SuperPred, and BindingDB databases were screened to identify Brevilin A targets. Lung cancer-related targets were sourced from GEO, Genecards, OMIM, TTD, and Drugbank databases. Utilizing Cytoscape software, a protein-protein interaction (PPI) network was established. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene set enrichment analysis (GSEA), and gene-pathway correlation analysis were conducted using R software. To validate network pharmacology results, molecular docking, molecular dynamics simulations, and in vitro experiments were performed. RESULTS: We identified 599 Brevilin A-associated targets and 3864 lung cancer-related targets, with 155 overlapping genes considered as candidate targets for Brevilin A against lung cancer. The PPI network highlighted STAT3, TNF, HIF1A, PTEN, ESR1, and MTOR as potential therapeutic targets. GO and KEGG analyses revealed 2893 enriched GO terms and 157 enriched KEGG pathways, including the PI3K-Akt signaling pathway, FoxO signaling pathway, and HIF-1 signaling pathway. GSEA demonstrated a close association between hub genes and lung cancer. Gene-pathway correlation analysis indicated significant associations between hub genes and the cellular response to hypoxia pathway. Molecular docking and dynamics simulations confirmed Brevilin A's interaction with PTEN and HIF1A, respectively. In vitro experiments demonstrated Brevilin A-induced dose- and time-dependent cell death in A549 cells. Notably, Brevilin A treatment significantly reduced HIF-1α mRNA expression while increasing PTEN mRNA levels. CONCLUSIONS: This study demonstrates that Brevilin A exerts anti-cancer effects in treating lung cancer through a multi-target and multi-pathway manner, with the HIF pathway potentially being involved. These results lay a theoretical foundation for the prospective clinical application of Brevilin A.

Pancancer analysis of the prognostic and immunological role of FANCD2: a potential target for carcinogenesis and survival.

Recent evidence has shed light on the significant role of FANCD2 in cancer initiation, development, and progression. However, a comprehensive pan-cancer analysis of FANCD2 has been lacking. In this study, we have conducted a thorough investigation into the expression profiles and prognostic significance of FANCD2, as well as its correlation with clinicopathological parameters and immune cell infiltration, using advanced bioinformatic techniques. The results demonstrate that FANCD2 is significantly upregulated in various common cancers and is associated with prognosis. Notably, higher expression levels of FANCD2 are linked to poor overall survival, as indicated by Cox regression and Kaplan-Meier analyses. Additionally, we have observed a decrease in the methylation of FANCD2 DNA in some cancers, and this decrease is inversely correlated with FANCD2 expression. Genetic alterations in FANCD2 predominantly manifest as mutations, which are associated with overall survival, disease-specific survival, disease-free survival, and progression-free survival in certain tumor types. Moreover, FANCD2 exhibits a strong correlation with infiltrating cell levels, immune checkpoint genes, tumor mutation burden (TMB), and microsatellite instability (MSI). Enrichment analysis further highlights the potential impact of FANCD2 on Fanconi anemia (FA) pathway and cell cycle regulation. Through this comprehensive pan-cancer analysis, we have gained a deeper understanding of the functions of FANCD2 in oncogenesis and metastasis across different types of cancer.

[Chemical constituents from flowers of Gardenia jasminoides].

OBJECTIVE: To study the chemical constituents from flowers of Gardenia jasminoides. METHODS: The compounds were isolated and purified by column chromatography and their structures were elucidated through spectroscopic techniques (NMR) and physicochemical properties. RESULTS: 15 compounds were isolated from flowers of G. jasminoides, and identified as 5, 7, 3'-trihydroxy-6, 4', 5'-trimethoxyflavone (1), 5, 7, 3', 5'- tetrahydroxy-6, 4'-dymethoxyflavone (2), kaempferol (3), quercetin (4), 3beta,23- dihydroxyurs-12-en-28-oic acid (5), 3beta,19alpha-dihydroxy-urs-12-en-28-oic acid (6), 3beta,19alpha,23-trihydroxy-urs-12-en-28-oic acid (7), emodin (8), physcion (9), crocin-I (10), beta-daucosterol (11), beta-sitosterol (12), stearic acid (13), palmitic acid (14), oleic acid (15). CONCLUSION: Compounds 1 - 15 are isolated from flowers of G. jasminoides and compounds 5 and 6 are isolated from genus Gardenia for the first time.

Application of cellulose to chromatographic media: Cellulose dissolution, and media fabrication and derivatization.

As the cornerstone of chromatographic technology, the development of high-performance chromatographic media is a crucial means to enhance the purification efficiency of biological macromolecules. Cellulose is a popular biological separation medium due to its abundant hydroxyl group on the surface, easy modification and, weak non-specific adsorption. In this paper, the development of cellulosic solvent systems, typical preparation methods of cellulosic chromatographic media, and the enhancement of chromatographic properties of cellulosic chromatographic media by polymeric ligand grafting strategies and their mechanism of action are reviewed. Ultimately, based on the current research status, a promising outlook for the preparation of high-performance cellulose-based chromatographic media was presented.

MIL-101(Fe) based biomass as permeable reactive barrier applied to EK-PRB remediation of antimony contaminated soil.

Numerous studies have demonstrated that electrokinetic-permeable reactive barrier (EK-PRB) can be used for the remediation of heavy metal contaminated soils, and their remediation efficiency is mainly determined by the filler material selected. By growing MIL-101(Fe) in situ on hollow loofah fiber (HLF), a novel material entitled HLF@MIL-101(Fe) was developed. The morphological characteristics and loading conditions were investigated, the adsorption characteristics were analyzed, and finally the synthesized composite material was applied to treat antimony-contaminated soil with EK-PRB as the reaction medium. The results show that MIL-101(Fe) is stably loaded on HLF. The adsorption capacity of Sb(III) can reach up to 82.31 mg g-1, and the adsorption is in accordance with the quasi-secondary kinetic model, which indicates that chemisorption is dominant. The isothermal adsorption model indicates that the adsorption form of HLF@MIL-101(Fe) is mainly monolayer adsorption with more uniform adsorption binding energy. In the EK-PRB experiment, when ethylenediaminetetraacetic acid (EDTA) is used as the cathodic electrolyte, it can effectively enhance the electromigration and electroosmotic effects, and the overall remediation efficiency of the soil is increased by 38.12% compared with the citric acid (CA) group. These demonstrate the feasibility of HLF@MIL-101(Fe) in collaboration with EK-PRB in the treatment of antimony-contaminated soil.

A Review on Electrospinning as Versatile Supports for Diverse Nanofibers and Their Applications in Environmental Sensing.

Rapid industrialization is accompanied by the deterioration of the natural environment. The deepening crisis associated with the ecological environment has garnered widespread attention toward strengthening environmental monitoring and protection. Environmental sensors are one of the key technologies for environmental monitoring, ultimately enabling environmental protection. In recent decades, micro/nanomaterials have been widely studied and applied in environmental sensing owing to their unique dimensional properties. Electrospinning has been developed and adopted as a facile, quick, and effective technology to produce continuous micro- and nanofiber materials. The technology has advanced rapidly and become one of the hotspots in the field of nanomaterials research. Environmental sensors made from electrospun nanofibers possess many advantages, such as having a porous structure and high specific surface area, which effectively improve their performance in environmental sensing. Furthermore, by introducing functional nanomaterials (carbon nanotubes, metal oxides, conjugated polymers, etc.) into electrospun fibers, synergistic effects between different materials can be utilized to improve the catalytic activity and sensitivity of the sensors. In this review, we aimed to outline the progress of research over the past decade on electrospinning nanofibers with different morphologies and functional characteristics in environmental sensors.

Centipeda minima active components and mechanisms in lung cancer.

BACKGROUND: Traditional Chinese medicine (TCM) has been extensively used for neoplasm treatment and has provided many promising therapeutic candidates. We previously found that Centipeda minima (C. minima), a Chinese medicinal herb, showed anti-cancer effects in lung cancer. However, the active components and underlying mechanisms remain unclear. In this study, we used network pharmacology to evaluate C. minima active compounds and molecular mechanisms in lung cancer. METHODS: We screened the TCMSP database for bioactive compounds and their corresponding potential targets. Lung cancer-associated targets were collected from Genecards, OMIM, and Drugbank databases. We then established a drug-ingredients-gene symbols-disease (D-I-G-D) network and a protein-protein interaction (PPI) network using Cytoscape software, and we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses using R software. To verify the network pharmacology results, we then performed survival analysis, molecular docking analysis, as well as in vitro and in vivo experiments. RESULTS: We identified a total of 21 C. minima bioactive compounds and 179 corresponding targets. We screened 804 targets related to lung cancer, 60 of which overlapped with C. minima. The top three candidate ingredients identified by D-I-G-D network analysis were quercetin, nobiletin, and beta-sitosterol. PPI network and core target analyses suggested that TP53, AKT1, and MYC are potential therapeutic targets. Moreover, molecular docking analysis confirmed that quercetin, nobiletin, and beta-sitosterol, combined well with TP53, AKT1, and MYC respectively. In vitro experiments verified that quercetin induced non-small cell lung cancer (NSCLC) cell death in a dose-dependent manner. GO and KEGG analyses found 1771 enriched GO terms and 144 enriched KEGG pathways, including a variety of cancer related pathways, the IL-17 signaling pathway, the platinum drug resistance pathway, and apoptosis pathways. Our in vivo experimental results confirmed that a C. minima ethanol extract (ECM) enhanced cisplatin (CDDP) induced cell apoptosis in NSCLC xenografts. CONCLUSIONS: This study revealed the key C. minima active ingredients and molecular mechanisms in the treatment of lung cancer, providing a molecular basis for further C. minima therapeutic investigation.

Waste lignin-based cationic flocculants treating dyeing wastewater: Fabrication, performance, and mechanism.

Lignin is often considered to be a complex polymeric structural material with excellent scalability. Reduced pressure distillation, a novel effective way, was proposed to recover reusable waste lignin from textile degumming black liquor. The structure of the recovered material was determined by Fourier Transform Infrared Spectroscopy (FT-IR), Gel Permeation Chromatography (GPC) and Klason Component Analysis. Recycled lignin (RL) was used as the basis for the synthesis of a cationic recycled lignin-based polymers (CRLM) through graft polymerizing cationic monomer (DMC). The optimum synthesis conditions were obtained by conducting orthogonal experiments using the cationicity as the studied parameter, while selecting pH, DMC/RL, reaction temperature and time as independent variables. Recovery experiments showed that the maximum recovery concentration of RL in the black liquor was 5 g/L, with a purity of approximately 83 %. Orthogonal experiments showed that a low DMC/RL ratio was crucial for the synthesis of flocculants. When the molar ratio of DMC/RL was 3:1, the cationicity of the prepared CRLM was as high as 11.32 %. Zeta potential and decolorization experiments also confirmed the stable decolorization performance of CRLM in three kinds of anionic dye wastewater. The experimental results showed that charge neutralization, chemical bonding forces and auxiliary effects play great role to remove anionic dyes, resulting in 94 %, 89 % and 94.9 % removal against Reactive Red 195 (RR195), Acid Red 18 (AR18) and Direct 168 (DB168) respectively. Therefore, this study demonstrated the potential of using recycled waste lignin as synthesize lignin-based flocculants in the field of printing and dyeing wastewater by treating waste with waste.

Iron cobalt-doped carbon nanofibers anode to simultaneously boost bioelectrocatalysis and direct electron transfer in microbial fuel cells: Characterization, performance, and mechanism.

A self-supporting electrode (FeCo-MOF/CNFs) combining iron cobalt bimetallic metal-organic frameworks (FeCo-MOFs) with carbon nanofibers (CNFs) was applied as the anode of a microbial fuel cell (MFC). The introduction of FeCo-MOFs enhanced graphitization degree and electrical conductivity, which endowed FeCo-MOF/CNFs with excellent electrocatalytic performance and good biocompatibility. The hierarchical porous structure of FeCo-MOF/CNFs provided abundant attachment sites for electroactive bacteria (EAB) and facilitated rapid electron transfer. The MFC equipped with FeCo-MOF/CNFs anode (FeCo/CNFs-MFC) exhibited considerable power generation output (maximum power density: 5.3 ± 0.2 W/m2, coulombic efficiency: 54 ± 4 %). In addition, FeCo/CNFs-MFC achieved a direct electron transfer (DET) catalytic current density of 0.63 A/m2. FeCo-MOF/CNFs could simultaneously enhance the bioelectrocatalysis activity and promote the DET process of EAB, which provided an effective way to improve the sluggish extracellular electron transport process of the MFC anode.

PFDN2 promotes cell cycle progression via the hnRNPD-MYBL2 axis in gastric cancer.

Gastric cancer (GC) is a major health burden worldwide, but our understanding of GC is limited, and the prognosis is poor. Novel therapeutic strategies and biomarkers are urgently needed to improve GC patient outcomes. Previously, we identified PFDN2 as a novel key gene in gastric cancer based on its differential expression between cancer and normal tissues. However, the role and underlying mechanisms of PFDN2 in GC remain elusive. In this article, we demonstrated that PFDN2 is highly expressed in GC and that upregulation of PFDN2 is associated with the progression of GC. We further found that PFDN2 could promote cell cycle progression by promoting MYBL2 expression. Mechanistically, we demonstrated that PFDN2 could upregulate MYBL2 expression by facilitating the nuclear translocation of hnRNPD, and thus promoting MYBL2 transcriptional program. In conclusion, we found that PFDN2 promotes cell cycle progression via the hnRNPD-MYBL2 axis and may serve as a potential biomarker and therapeutic target for GC.

BGN/FAP/STAT3 positive feedback loop mediated mutual interaction between tumor cells and mesothelial cells contributes to peritoneal metastasis of gastric cancer.

Peritoneal metastasis (PM) is most frequent in gastric cancer (GC) and cancer-associated fibroblasts (CAFs) play a critical role in this process. However, the concrete mechanism of crosstalk between CAFs and cancer cells in PM of GC remains unclear. Microarray sequencing of GC focus and PM lesions was performed, and biglycan (BGN) was screened for further study. Clinically, BGN expression was higher in GC tissues than adjacent normal tissues, and high expression correlated with poor prognosis. In vitro experiments demonstrated that BGN promoted tumor progression and the transformation of mesothelial cells (MCs) into cancer-associated fibroblasts like cells (CAFLCs). In turn, CAFLCs-derived fibroblast activation protein (FAP) facilitated the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of GC cells. GC-derived BGN combined with toll like receptor 2 (TLR2)/TLR4 on MCs to activate the NF-κB pathway and promote the transformation of MCs into CAFLCs by the recovery experiment, coimmunoprecipitation assay, nuclear and cytoplasmic protein extraction assay. CAFLCs-derived FAP could activate the JAK2/STAT3 signaling pathway in GC. Finally, activated STAT3 promoted BGN transcription in GC, resulting in a BGN/FAP-STAT3 positive feedback loop. Taken together, mutual interaction between tumor cells and activated MCs mediated by a BGN/FAP-STAT3 positive feedback loop facilitates PM of GC and provides a potential biomarker and therapeutic target for GC metastasis.

Carbon-doped WO3 electrochemical aptasensor based on Box-Behnken strategy for highly-sensitive detection of tetracycline.

Aptamer has been proved to be an important probe for antibiotic detection. Here, the electrical signal was doubly amplified by the synergistic effect of C-WO3 and AuNPs. The probe structure has a specific recognition effect on tetracycline, which improves the selectivity and anti-interference of the sensor. With the assistance of BBD strategy, the experimental errors of the C-WO3@AuNPs aptasensor were reduced and the best conditions for its preparation were obtained. This was conducive to obtain the best electrical signal transmission capacity of the electrode, greatly improved the sensor sensitivity. Under this mechanism, the antibiotic sensor achieved a low detection range (0.1 nM-100 nM) and a low detection limit (4.8 × 10-2 nM). The sensor showed excellent selectivity even in the presence of coexisting pollutants. This work explored the mechanism of charge change and demonstrated the role of probes in antibiotic sensing, providing important prospects of future applications in electrochemical sensors.

Preparation of cellulose-based chromatographic medium for biological separation: A review.

Cellulose is a kind of renewable linear polysaccharide with good safety, hydrophilicity, biocompatibility and biodegradability and has become a commonly used chromatographic medium for biological separation and purification. The purpose of this paper is to describe the recent developments in the applications of cellulose-based absorbents as chromatographic medium. This review also attempts to explain the current situation of cellulose-based chromatographic medium from the aspects of cellulose dissolution, common strategies for generating spherical particles of cellulose and its derivatives (such as titration, emulsification, microfluidic and other synthesis methods) and improvement of adsorption properties. Furthermore, non-spherical cellulose-based chromatographic medium are also described briefly.

Identification of Marek's disease virus pUL56 homologue and analysis of critical amino acid stretches indispensable for its intracellular localization.

Marek's disease virus (MDV) is considered a unique member of the Alphaherpesvirinae subfamily that induces rapid onset of T cell lymphoma in chickens. Compared with other conserved UL56 gene homologues of herpesviruses, little is known about the roles of MDV UL56 gene, while recent studies of mammalian herpesvirus pUL56 proteins have revealed their involvement in promoting ubiquitination of the Nedd4 (neural precursor cell expressed developmentally down-regulated protein 4) -like E3 ubiquitin ligases for proteasomal degradation and in modulating host immune responses. To determine the expression kinetics of UL56 gene products, chicken embryo fibroblasts were infected with very virulent or attenuated MDV strain and analyzed by quantitative PCR and Western blotting. During the time course of infection, the levels of UL56 mRNA transcripts increased consistently. At the translational level, the pUL56 protein encoded by UL56 gene was expressed in the size of 32 kDa, which emerged as early as 12 h post-infection (hpi) but otherwise began to wane at 72 hpi thereafter. With the treatment of viral DNA synthesis inhibitors, the pUL56 expression was significantly reduced, featuring the dynamics of a late (γ)-gene product. By confocal imaging, pUL56 was found to reside in the Golgi compartment. Both the L-domain motifs and the C-terminal tail-anchored transmembrane were essential for its intracellular localization. Noticeably, pUL56 co-localized with a truncated mutant of the chicken Nedd4-like family protein harboring only the WW domains; however, co-immunoprecipitation assay established no direct interaction between them, and the ectopic expression of pUL56 did not alter the abundance of endogenous Nedd4-like protein. Overall, the present study provides a caveat that the pUL56 homologues of different herpesviruses with structural similarities might vary in expression patterns and probably in functional consequences. For this reason, further investigation should be encouraged to focus on the potential association between UL56 gene and MDV pathogenesis in the context of engineered viral mutants.

Research progress in external field intensification of forward osmosis process for water treatment: A critical review.

Forward osmosis (FO) is an emerging permeation-driven membrane technology that manifests advantages of low energy consumption, low operating pressure, and uncomplicated engineering compared to conventional membrane processes. The key issues that need to be addressed in FO are membrane fouling, concentration polarization (CP) and reverse solute diffusion (RSD). They can lead to problems about loss of draw solutes and reduced membrane lifetime, which not only affect the water treatment effectiveness of FO membranes, but also increase the economic cost. Current research has focused on FO membrane preparation and modification strategies, as well as on the selection of draw solutions. Unfortunately, these intrinsic solutions had limited success in unraveling these phenomena. In this paper, we provide a brief review of the current state of research on existing external field-assisted FO systems (including electric-, pressure-, magnetic-, ultrasonic-, light- and flow-assisted FO system), analyze their mitigation mechanisms for the above key problems, and explore potential research directions to aid in the further development of FO systems. This review aims to reveal the feasibility of the development of external field-assisted FO technology to achieve a more economical and efficient FO treatment process.

SNHG16 upregulation-induced positive feedback loop with YAP1/TEAD1 complex in Colorectal Cancer cell lines facilitates liver metastasis of colorectal cancer by modulating CTCs epithelial-mesenchymal transition.

Circulating tumor cells (CTCs) are important precursors of colorectal cancer (CRC) metastasis. The epithelial-mesenchymal transition (EMT) process facilitates CTC invasion by allowing these cells to evade antimetastatic checkpoints to mediate distant metastasis. However, the specific molecular mechanism of tumor EMT remains largely unknown. Based on our previous research on the YAP1 pathway, we further studied the upstream molecule small nucleolar RNA host gene 16 (SNHG16), whose expression was correlated with advanced TNM stage, distant metastasis, and poor prognosis in CRC patients. Furthermore, loss- and gain-of-function assays revealed that SNHG16 promoted CRC colony formation, proliferation, migration, invasion, EMT, mesenchymal-like CTC generation, and liver metastasis through YAP1. Mechanistically, SNHG16 acted as a miRNA sponge to sequester miR-195-5p on Ago2, thereby protecting YAP1 from repression. Moreover, YAP1 bound TEA domain transcription factor 1 (TEAD1) to form a YAP1/TEAD1 complex, which in turn bound two sites in the promoter of SNHG16 and regulate SNHG16 transcription. Finally, in vivo experiments showed that the inhibition of SNHG16 suppressed tumor progression, and that YAP1 rescued the effect of SNHG16 on tumor progression. Herein, we have clarified a hitherto unexplored SNHG16-YAP1/TEAD1 positive feedback loop, that may be a candidate target for CRC treatment.