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Activation of a silent gene cluster in Streptomyces nodosus leads to synthesis of a cinnamoyl-containing non-ribosomal peptide (CCNP) that is related to skyllamycins. This novel CCNP was isolated and its structure was interrogated using mass spectrometry and nuclear magnetic resonance spectroscopy. The isolated compound is an oxidised skyllamycin A in which an additional oxygen atom is incorporated in the cinnamoyl side-chain in the form of an epoxide. The gene for the epoxide-forming cytochrome P450 was identified by targeted disruption. The enzyme was overproduced in Escherichia coli and a 1.43 Å high-resolution crystal structure was determined. This is the first crystal structure for a P450 that forms an epoxide in a substituted cinnamoyl chain of a lipopeptide. These results confirm the proposed functions of P450s encoded by biosynthetic gene clusters for other epoxidized CCNPs and will assist investigation of how epoxide stereochemistry is determined in these natural products.
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Sistema Enzimático do Citocromo P-450 , Depsipeptídeos , Streptomyces , Sistema Enzimático do Citocromo P-450/química , Peptídeos Cíclicos/químicaRESUMO
Glycosylated polyene macrolides are important antifungal agents that are produced by many actinomycete species. Development of new polyenes may deliver improved antibiotics. Here, Streptomyces nodosus was genetically re-programmed to synthesise pentaene analogues of the heptaene amphotericin B. These pentaenes are of interest as surrogate substrates for enzymes catalysing unusual, late-stage biosynthetic modifications. The previous deletion of amphotericin polyketide synthase modules 5 and 6 generated S. nodosus M57, which produces an inactive pentaene. Here, the chain-terminating thioesterase was fused to module 16 to generate strain M57-16TE, in which cycles 5, 6, 17 and 18 are eliminated from the biosynthetic pathway. Another variant of M57 was obtained by replacing modules 15, 16 and 17 with a single 15-17 hybrid module. This gave strain M57-1517, in which cycles 5, 6, 15 and 16 are deleted. M57-16TE and M57-1517 gave reduced pentaene yields. Only M57-1517 delivered its predicted full-length pentaene macrolactone in low amounts. For both mutants, the major pentaenes were intermediates released from modules 10, 11 and 12. Longer pentaene chains were unstable. The novel pentaenes were not glycosylated and were not active against Candida albicans. However, random mutagenesis and screening may yet deliver new antifungal producers from the M57-16TE and M57-1517 strains.
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Anfotericina B , Policetídeo Sintases , Anfotericina B/farmacologia , Policetídeo Sintases/genética , Policetídeo Sintases/metabolismo , Polienos/metabolismo , Antifúngicos/farmacologia , Antifúngicos/metabolismo , Macrolídeos/metabolismo , AntibacterianosRESUMO
The accumulation of reducing sugars in cold-stored tubers, known as cold-induced sweetening (CIS), negatively affects potato processing quality. The starch to sugar interconversion pathways that are altered in cold-stored CIS tubers have been elucidated, but the mechanism that regulates them remains largely unknown. This study identified a CBF/DREB transcription factor (StTINY3) that enhances CIS resistance by both activating starch biosynthesis and repressing the hydrolysis of sucrose to reducing sugars in detached cold-stored tubers. Silencing StTINY3 in a CIS-resistant genotype decreased CIS resistance, while overexpressing StTINY3 in a CIS-sensitive genotype increased CIS resistance, and altering StTINY3 expression was associated with expression changes in starch resynthesis-related genes. We showed first that overexpressing StTINY3 inhibited sucrose hydrolysis by enhancing expression of the invertase inhibitor gene StInvInh2, and second that StTINY3 promoted starch resynthesis by up-regulating a large subunit of the ADP-glucose pyrophosphorylase gene StAGPaseL3, and the glucose-6-phosphate transporter gene StG6PT2. Using electrophoretic mobility shift assays, we revealed that StTINY3 is a nuclear-localized transcriptional activator that directly binds to the dehydration-responsive element/CRT cis-element in the promoters of StInvInh2 and StAGPaseL3. Taken together, these findings established that StTINY3 influences CIS resistance in cold-stored tubers by coordinately modulating the starch to sugar interconversion pathways and is a good target for improving potato processing quality.
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Solanum tuberosum , Carboidratos , Temperatura Baixa , Hidrólise , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Tubérculos/metabolismo , Solanum tuberosum/metabolismo , Amido/metabolismo , Sacarose/metabolismo , Açúcares/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
Harmful algal blooms have deleterious effects on aquatic ecosystems and human health. The application of algicidal bacteria is a promising and environmentally friendly method of preventing and eradicating harmful algal blooms. In this study, a screen for algicidal agents against harmful algal blooms was used to identify an algicidal bacterial strain (strain O-4) isolated from a Karenia mikimotoi culture. Strain O-4 exhibited a strong inhibitory effect on harmful K. mikimotoi and was identified as Paracoccus homiensis via 16S rRNA gene sequence analysis. This strain killed K. mikimotoi by secreting active algicidal compounds, which were stable at temperatures of -80-121 °C but were sensitive to strongly acidic conditions (pH = 2). The algicidal properties of strain O-4 against K. mikimotoi were cell density- and time-dependent. No significant changes or negative effects were noted for two other Chlorophyta species, which highlighted the specificity of the studied algicidal substance. Finally, single-factor experiments revealed the optimum growth conditions of strain O-4 under different pH and temperature conditions. Therefore, strain O-4 has the potential to be used as a bio-agent for reducing the biomass of harmful K. mikimotoi blooms.
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Dinoflagellida , Proliferação Nociva de Algas , Bactérias , Ecossistema , Humanos , Paracoccus , RNA Ribossômico 16S/genética , Microbiologia da ÁguaRESUMO
Mink Aleutian disease (AMD) is the first of the three major diseases of fur animals. It is a common immunosuppressive disease in mink farms worldwide, which seriously endangers the development of the mink farming industry. Strengthening the understanding of the positive serum rate and spatial distribution of AMD is of great significance for the prevention and control of disease caused by the Aleutian virus. Therefore, we conducted a systematic review and meta-analysis to assess the seroprevalence of AMD in China. We extracted 45 studies related to the seroprevalence of Chinese AMD, with samples taken between 1981 and 2017. Our systematic review and meta-analysis results show that, during the selected period, the overall positive rate of AMD in China was 55.3% (95% CI 48.5-62.0). The results from subgroups analysis of the potential risk factors showed that the seroprevalence rate of AMD in China in the past 36 years rose from 48% (95% CI 37.0-60.5) in 1981-2009 to 61.4% (95% CI 43.6-79.3) in 2010-2017. The date of the spatial difference in AMD seroprevalence indicated that AMD seroprevalence was unevenly distributed in different regions: the number of mink in eastern China and northeastern China was relatively high, and the seroprevalence rates were 57.9%, (95% CI 46.2-69.7) and 61.3% (95% CI 53.1-69.5), respectively. Central China had the highest seroprevalence rate of AMD at 69.8% (95% CI 64.4-75.2). At the provincial level, the AMD seroprevalence rate in Jiangsu was as high as 96% (95% CI 94.1-97.8), and the AMD seroprevalence rate in Shaanxi was the lowest at 22.1% (95% CI 20.3-23.9). This suggested that the AMD seroprevalence rate in China was unevenly distributed. In other subgroups, the positive rate of AMD in adult mink was higher than in juvenile mink. This implied that the high prevalence of AMD in China was caused by multiple factors. The meta-regression results indicated that the detection method subgroup (P = 0.008) may be the source of heterogeneity. Our data system evaluated the prevalence of Aleutian disease in China in the last 37 years and a preliminary discussion on the risk factors of AMD. It may help prevent and control AMD in China. It is recommended to conduct further epidemiological testing and develop a comprehensive testing plan to determine the risk factors associated with Aleutian disease and improve the Aleutian disease control strategy.
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Vírus da Doença Aleutiana do Vison/isolamento & purificação , Doença Aleutiana do Vison/sangue , Doença Aleutiana do Vison/epidemiologia , Doença Aleutiana do Vison/virologia , Vírus da Doença Aleutiana do Vison/classificação , Vírus da Doença Aleutiana do Vison/genética , Vírus da Doença Aleutiana do Vison/imunologia , Animais , Anticorpos Antivirais/sangue , China/epidemiologia , Feminino , Masculino , Vison/imunologia , Vison/virologia , Filogenia , Prevalência , Estudos SoroepidemiológicosRESUMO
Eutrophication is a global problem, and bacterial diversity and community composition are usually affected by eutrophication. However, limited information on the ecological significance of bacterial community during algae blooms of rivers has been given, more studies should be focused on the bacterial diversity and distribution characteristics in eutrophic rivers. In this study, we explored the spatial variations of bacterial biomass, community structure, and their relationship with environmental factors in the eutrophic Xiangxi River. The content of Chlorophyll (Chl) was about 16 mg/L in the midstream (S2, S3), which was in the range of light eutrophication. Significant spatial variation of bacterial community structure was found at different sites and depths (p < 0.05), and the driving environmental factor was found to be nitrogen, mainly detected as total nitrogen (TN), Kjeldahl nitrogen (KN), and ammonia nitrogen (NH4+) (p < 0.05). The midstream sites had some significantly different bacteria, including algicidal bacteria and dominant lineages during algal blooms. This result was consistent with the functional prediction, where significant higher abundance of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was associated with algicidal substances in the midstream. At different water depths, some populations adapted to the surface layer, such as the class Flavobacteriia, and others preferred to inhabit in the bottom layer, such as Betaproteobacteria and Acidobacteria. The bacterial biomass was higher in the bottom layer than that in the surface and middle layer, and temperature and pH were found to be the major driving factors. The bacterial diversity increased with the increasing of depths in most sampling sites according to operational taxonomic units (OTUs), Chao1 and ACE indexes, and PO43- was demonstrated to be the most significant factor. In summary, this study offered the evidence for microbial distribution characteristics across different sites and depths in summer, and its relationship with environmental variables in a eutrophic river.
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Monitoramento Ambiental/métodos , Eutrofização , Microbiota , Proteobactérias/crescimento & desenvolvimento , Rios , Biomassa , China , Clorofila/análise , Microbiota/genética , Nitrogênio/análise , Fosfatos/análise , Fósforo/análise , Proteobactérias/classificação , Proteobactérias/genética , RNA Ribossômico 16S/genética , Rios/química , Rios/microbiologia , Estações do Ano , TemperaturaRESUMO
In this study, we report the characteristics of a microbial community in sampled groundwater and elucidate the effects of temperature and pH disturbances on bacterial structure and nitrogen-cycling functions. The predominant phyla of candidate OD1, candidate OP3, and Proteobacteria represented more than half of the total bacteria, which clearly manifested as a "low nucleic acid content (LNA) bacteria majority" type via flow cytometric fingerprint. The results showed that LNA bacteria were more tolerant to rapid changes in temperature and pH, compared to high nucleic acid content (HNA) bacteria. A continuous temperature increase test demonstrated that the LNA bacterial group was less competitive than the HNA bacterial group in terms of maintaining their cell intactness and growth potential. In contrast, the percentage of intact LNA bacteria was maintained at nearly 70% with pH decrease, despite a 50% decrease in total intact cells. Next-generation sequencing results revealed strong resistance and growth potential of phylum Proteobacteria when the temperature increased or the pH decreased in groundwater, especially for subclasses α-, ß-, and γ-Proteobacteria. In addition, relative abundance of nitrogen-related functional genes by qPCR showed no difference in nitrifiers or denitrifiers within 0.45 µm-captured and 0.45 µm-filterable bacteria due to phylogenetic diversity. One exception was the monophyletic anammox bacteria that belong to the phylum Planctomycetes, which were mostly captured on a 0.45-µm filter. Furthermore, we showed that both temperature increase and pH decrease could enhance the denitrification potential, whereas the nitrification and anammox potentials were weakened.
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Bactérias/isolamento & purificação , Água Subterrânea/química , Água Subterrânea/microbiologia , Bactérias/classificação , Bactérias/genética , Bactérias/metabolismo , Biodiversidade , Desnitrificação , Citometria de Fluxo , Concentração de Íons de Hidrogênio , Nitrificação , Nitrogênio/metabolismo , Filogenia , TemperaturaRESUMO
Infrared photodetectors are gaining remarkable interest due to their widespread civil and military applications. Low-dimensional materials such as quantum dots, nanowires, and two-dimensional nanolayers are extensively employed for detecting ultraviolet to infrared lights. Moreover, in conjunction with plasmonic nanostructures and plasmonic waveguides, they exhibit appealing performance for practical applications, including sub-wavelength photon confinement, high response time, and functionalities. In this review, we have discussed recent advances and challenges in the prospective infrared photodetectors fabricated by low-dimensional nanostructured materials. In general, this review systematically summarizes the state-of-the-art device architectures, major developments, and future trends in infrared photodetection.
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Ocean acidification (OA) driven by elevated carbon dioxide (CO2) levels is expected to disturb marine ecological processes, including the formation and control of harmful algal blooms (HABs). In this study, the effects of rising CO2 on the allelopathic effects of macroalgae Ulva pertusa to a toxic dinoflagellate Karenia mikimotoi were investigated. It was found that high level of CO2 (1000 ppmv) promoted the competitive growth of K. mikimotoi compared to the group of present ambient CO2 level (420ppmv), with the number of algal cell increased from 32.2 × 104 cells/mL to 36.75 × 104 cells/mL after 96 h mono-culture. Additionally, rising CO2 level weakened allelopathic effects of U. pertusa on K. mikimotoi, as demonstrated by the decreased inhibition rate (50.6 % under the original condition VS 34.3 % under the acidified condition after 96 h co-culture) and the decreased reactive oxygen species (ROS) level, malondialdehyde (MDA) content, antioxidant enzymes activity (superoxide dismutase (SOD), peroxidase (POD), glutathione peroxidase (GPX), glutathione reductase (GR) and catalase (CAT) and non-enzymatic antioxidants (glutathione (GSH) and ascorbic acid (ascorbate, vitamin C). Indicators for cell apoptosis of K. mikimotoi including decreased caspase-3 and -9 protease activity were observed when the co-cultured systems were under rising CO2 exposure. Furthermore, high CO2 level disturbed fatty acid synthesis in U. pertusa and significantly decreased the contents of fatty acids with allelopathy, resulting in the allelopathy weakening of U. pertusa. Collectively, rising CO2 level promoted the growth of K. mikimotoi and weakened allelopathic effects of U. pertusa on K. mikimotoi, indicating the increased difficulties in controlling K. mikimotoi using macroalgae in the future.
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Dinoflagellida , Alga Marinha , Dióxido de Carbono/toxicidade , Concentração de Íons de Hidrogênio , Água do Mar , Dinoflagellida/fisiologia , Proliferação Nociva de AlgasRESUMO
Chitosan samples were prepared from the shells of marine animals (crab and shrimp) and the cell walls of fungi (agaricus bisporus and aspergillus niger). Fourier-transform infrared spectroscopy (FT-IR) was used to detect their molecular structures, while headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) was employed to analyze their odor composition. A total of 220 volatile organic compounds (VOCs), including esters, ketones, aldehydes, etc., were identified as the odor fingerprinting components of chitosan for the first time. A principal component analysis (PCA) revealed that chitosan could be effectively identified and classified based on its characteristic VOCs. The sum of the first three principal components explained 87% of the total variance in original information. An orthogonal partial least squares discrimination analysis (OPLS-DA) model was established for tracing and source identification purposes, demonstrating excellent performance with fitting indices R2X = 0.866, R2Y = 0.996, Q2 = 0.989 for independent variable fitting and model prediction accuracy, respectively. By utilizing OPLS-DA modeling along with a heatmap-based tracing path study, it was found that 29 VOCs significantly contributed to marine chitosan at a significance level of VIP > 1.00 (p < 0.05), whereas another set of 20 VOCs specifically associated with fungi chitosan exhibited notable contributions to its odor profile. These findings present a novel method for identifying commercial chitosan sources, which can be applied to ensure biological safety in practical applications.
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Background: Deer tuberculosis is a chronic zoonotic infectious disease, despite the existence of socio-economic and zoonotic risk factors, but at present, there has been no systematic review of deer tuberculosis prevalence in mainland China. The aim of this meta-analysis was to estimate the overall prevalence of deer TB in mainland China and to assess possible associations between potential risk factors and the prevalence of deer tuberculosis. Methodology: This study was searched in six databases in Chinese and English, respectively (1981 to December 2023). Four authors independently reviewed the titles and abstracts of all retrieved articles to establish the inclusion exclusion criteria. Using the meta-analysis package estimated the combined effects. Cochran's Q-statistic was used to analyze heterogeneity. Funnel plots (symmetry) and used the Egger's test identifying publication bias. Trim-and-fill analysis methods were used for validation and sensitivity analysis. we also performed subgroup and meta-regression analyses. Results: In this study, we obtained 4,400 studies, 20 cross-sectional studies were screened and conducted a systematic review and meta-analysis. Results show: The overall prevalence of tuberculosis in deer in mainland China was 16.1% (95% confidence interval (CI):10.5 24.6; (Deer tuberculosis infected 5,367 out of 22,215 deer in mainland China) 5,367/22215; 1981 to 2023). The prevalence in Central China was the highest 17.5% (95% CI:14.0-21.9; 63/362), and among provinces, the prevalence in Heilongjiang was the highest at 26.5% (95% CI:13.2-53.0; 1557/4291). Elaphurus davidianus was the most commonly infected species, with a prevalence of 35.3% (95% CI:18.5-67.2; 6/17). We also assessed the association between geographic risk factors and the incidence of deer tuberculosis. Conclusion: Deer tuberculosis is still present in some areas of China. Assessing the association between risk factors and the prevalence of deer tuberculosis showed that reasonable and scientific-based breeding methods, a suitable breeding environment, and rapid and accurate detection methods could effectively reduce the prevalence of deer tuberculosis. In addition, in the management and operation of the breeding base, improving the scientific feed nutrition standards and establishing comprehensive standards for disease prevention, immunization, quarantine, treatment, and disinfection according to the breeding varieties and scale, are suggested as ways to reduce the prevalence of deer tuberculosis.
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Cyanobacterial blooms caused by eutrophication have become a major environmental problem in aquatic ecosystems worldwide over the last few decades. Phosphorus is a limiting nutrient that affects the growth of cyanobacteria and plays a role in dynamic changes in algal density and the formation of cyanobacterial blooms. Therefore, identifying the association between phosphorus sources and Microcystis, which is the most representative and harmful cyanobacteria, is essential for building an understanding of the ecological risks of cyanobacterial blooms. However, systematic reviews summarizing the relationships between Microcystis and phosphorus in aquatic environments are rare. Thus, this study provides a comprehensive overview of the physiological and ecological interactions between phosphorus sources and Microcystis in aquatic environments from the following perspectives: (i) the effects of phosphorus source and concentration on Microcystis growth, (ii) the impacts of phosphorus on the environmental behaviors of Microcystis, (iii) mechanisms of phosphorus-related metabolism in Microcystis, and (iv) role of Microcystis in the distribution of phosphorus sources within aquatic environments. In addition, relevant unsolved issues and essential future investigations (e.g., secondary ecological risks) have been highlighted and discussed. This review provides deeper insights into the relationship between phosphorus sources and Microcystis and can serve as a reference for the evaluation, monitoring, and effective control of cyanobacterial blooms.
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Cianobactérias , Microcystis , Microcystis/fisiologia , Fósforo , Ecossistema , Eutrofização , Lagos/microbiologiaRESUMO
Linoleic acid (LA) shows great potential in inhibiting the growth of multiple red tide microalgae by disturbing algal physio-biochemical processes. However, our knowledge on the mechanisms of algal mortality at metabolic level remains limited. Herein, the response of K. mikimotoi to LA was evaluated using metabolomics, stable isotope techniques (SIT), and physiological indicators. Results showed that 100 µg/L LA promoted the growth of K. mikimotoi, which was significantly inhibited by 500 µg/L LA, along with a significant reduction of photosynthetic pigments and a significant increase of reactive oxygen species (ROS). SIT showed that LA entered algal cells, and 56 isotopologues involved in ferroptosis, carotenoid biosynthesis, and porphyrin metabolism were identified. Non-targeted metabolomics identified 90 and 111 differential metabolites (DEMs) belonging to 11 metabolic pathways under the 500 µg/L and 100 µg/L LA exposure, respectively. Among them, 34 DEMs were detected by SIT. Metabolic pathway analysis showed that 500 µg/L LA significantly promoted ferroptosis, and significantly inhibited carotenoid biosynthesis, porphyrin metabolism, sphingolipid metabolism, and lipopolysaccharide biosynthesis, presenting changes opposite to those observed in 100 µg/L LA-treated K. mikimotoi. Overall, this study revealed the metabolic response of K. mikimotoi to LA, enriching our understanding on the allelochemical mechanism of LA on K. mikimotoi.
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Dinoflagellida , Porfirinas , Ácido Linoleico/metabolismo , Ácido Linoleico/farmacologia , Feromônios/metabolismo , Feromônios/farmacologia , Fotossíntese , Carotenoides/metabolismoRESUMO
Antibiotic resistance genes (ARGs) have caused widespread concern because of their potential harm to environmental safety and human health. As substitutes for conventional plastics, the toxic effects of short-term degradation products of biodegradable plastics (polylactic acid (PLA) and polyhydroxyalkanoates (PHA)) on bacteria and their impact on ARGs transfer were the focus of this study. After 60 days of degradation, more secondary nanoplastics were released from the biodegradable plastics PLA and PHA than that from the conventional plastics polystyrene (PS). All kinds of nanoplastics, no matter released from biodegradable plastics or conventional plastics, had no significant toxicity to bacteria. Nanoplastic particles from biodegradable plastics could significantly increase the transfer efficiency of ARGs. Although the amount of secondary nanoplastics produced by PHA microplastics was much higher than that of PLA, the transfer frequency after exposure to PLA was much higher, which may be due to the agglomeration of PHA nanoplastics caused by plastic instability in solution. After exposure to the 60 d PLA nanoplastics, the transfer frequency was the highest, which was approximately 28 times higher than that of control. The biodegradable nanoplastics significantly enhanced the expression of the outer membrane pore protein genes ompA and ompC, which could increase cell membrane permeability. The expression levels of trfAp and trbBp were increased by repressed major global regulatory genes korA, korB, and trbA, which eventually led to an increase in conjugative transfer frequency. This study provides important insights into the evaluation of the environmental and health risks caused by secondary nanoplastics released from biodegradable plastics.
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Plásticos Biodegradáveis , Plásticos , Humanos , Plásticos/toxicidade , Microplásticos/toxicidade , Poliestirenos , Poliésteres , BactériasRESUMO
Acidification of seawater resulting from absorption of excessive carbon dioxide from the atmosphere is posing a serious threat to marine ecosystem. In this study, we hypothesized that acidified seawater attenuates allelopathic effects of macroalgae on red tide algae because the increase of dissolved carbon dioxide benefits algal growth, and investigated the allelopathic effects of Ulva pertusa on Karenia mikimotoi in response to seawater acidification by determining cell density, photosynthetic pigment content, chlorophyll fluorescence parameters, and chloroplast structure of K. mikimotoi under U. pertusa stress in original (pH=8.2) and acidified (pH=7.8) seawater. U. pertusa inhibited the growth of K. mikimotoi in the original and acidizing seawater, and the inhibition rate was positively correlated with treatment time and concentration of U. pertusa. However, acidizing condition significantly weakened the inhibition degree of U. pertusa on K. mikimotoi (P < 0.05), with the inhibition rates decreased from 51.85 to 43.16% at 10 gFW/L U. pertusa for 96 h. U. pertusa reduced contents of chlorophyll a, chlorophyll c, and carotenoid, maximum photochemical quantum yield (Fv/Fm), actual quantum yield, maximum relative electron transfer efficiency (rETRmax) of PSII, real-time fluorescence value (F), and maximum fluorescence value (Fm') of PSII of K. mikimotoi under original and acidified conditions. And, the inhibition degree of U. pertusa under acidizing condition was significantly lower than that of original seawater group. Furthermore, the damage degree of chloroplast structure of K. mikimotoi under U. pertusa stress was more serious under original seawater condition. These results indicate that acidification of seawater attenuates the allelopathic effects of U. pertusa on K. mikimotoi.
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Dinoflagellida , Ulva , Clorofila A , Dióxido de Carbono , Ecossistema , Dinoflagellida/fisiologia , Água do Mar/química , Concentração de Íons de HidrogênioRESUMO
Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses. However, the underlying roles of EPS in the interactions between microplastics (MPs) and microalgae remain poorly understood. In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 µm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure. The results indicated that the EPS compositions were different when M. aeruginosa was exposed to PS MPs of different sizes. The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress. With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 µm PS MPs to the algal surface, which could destroy the cell wall. In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively. High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa. Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
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Microalgas , Microcystis , Poluentes Químicos da Água , Microplásticos/toxicidade , Microcystis/metabolismo , Poliestirenos/metabolismo , Matriz Extracelular de Substâncias Poliméricas , Plásticos/metabolismo , Microalgas/metabolismo , Poluentes Químicos da Água/metabolismoRESUMO
Previously, we researched that bovine viral diarrhea virus (BVDV) induced a very significant increase in fibroblast growth factor 21 (FGF21) expression in mouse liver and that FGF21 was increased in the peripheral blood of BVD cattle and BVD mice. To determine the role of FGF21 in relieving clinical symptoms and inhibiting the intestinal damage caused by BVDV in BVD development in mice, BALB/c mice were intraperitoneally injected with cytopathic biotype (cp) BVDV-LS01 (isolated and identified by our group) to establish a BVD mouse model. The role of FGF21 in the BVD mouse model was investigated by injecting the mice with FGF21. The animals were divided into control, BVDV challenge, BVDV + FGF21, BVDV + FGF21Ab (anti-FGF21 antibody), and BVDV + IgG (immunoglobulin G) groups. The stool consistency, the degree of bloody diarrhea, histopathological changes, inflammatory cell infiltration, weight loss percentage, and detection of BVDV in the feces of the mice were examined, and the pathological changes and inflammatory cytokine expression were analyzed. The results showed that after BVDV challenge, the average BVD mouse model score of the BVDV mice was 11.6 points. In addition to mild diarrhea and tissue damage, BVDV was detected in the stools of 13 BVDV mice. Only two mice in the control group had scores (both, 1 point each). The comprehensive scoring results demonstrated the successful establishment of the BVD mouse model. FGF21 alleviated the clinical symptoms in the BVD mice and significantly improved weight loss. Furthermore, FGF21 inhibited the BVDV-induced leukocyte, platelet, and lymphocyte reduction while inhibiting the expression of BVDV-induced inflammatory factors. In the BVD mice, FGF21 promoted duodenal epithelial cell proliferation, thereby significantly improving the damage to the cells. In conclusion, FGF21 exerted a good therapeutic effect on the BVD mouse model.
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In this study, the SNP-TiO2@Cu-MOF composite was prepared successfully by loading non-noble metal modified TiO2 (SNP-TiO2) on the surface of copper metal organic skeleton (Cu-MOF), and compared the inactivation efficiency of different photocatalysts to Karenia mikimotoi (K. mikimotoi) under visible light. The obtained photocatalyst had the characteristic crystal faces of Cu-MOF and SNP- TiO2, and contained functional groups such as Cu-O, -COOH, N-O, P-O, etc., which indicated the structural stability of the photocatalyst. The band gap of SNP-TiO2@Cu-MOF composite was 2.82 eV, and it had great light absorption ability in visible light region. It was proved to be a mesoporous adsorption material, which had a huge specific surface area (245 m2/g). Compared with other photocatalysts, SNP-TiO2@Cu-MOF composite showed the strongest photocatalytic activity. When the concentration of composite material was set to 100 mg/L and the exposure time was 6 h, the visible light photocatalytic inactivation efficiency of K. mikimotoi was 93.75 %. By measuring various metabolic indexes of K. mikimotoi under the action of different photocatalysts for 1 h, it was confirmed that cell inactivation was due to the increased membrane permeability and degradation of photosynthetic pigments and main life proteins. This research showed that SNP-TiO2@Cu-MOF composite material was full of great potential and application prospect in controlling the outbreak of eutrophication.
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Cobre , Estruturas Metalorgânicas , Cobre/química , Catálise , LuzRESUMO
Carbon-based all-inorganic perovskite solar cells (C-IPSCs) are stable, upscalable and have low CO2-footprint to fabricate. However, they are inefficient in converting light to electricity due to poor hole extraction at perovskite/carbon interface. Here we enable an efficient hole extraction in C-IPSCs with the aid of inorganic p-type nickel oxide nanoparticles (NiOx-NPs) at the interface and in carbon. By tailoring the work function (WF) of carbon, and reducing the energy-level misalignment at the perovskite/carbon interface, NiOx-NPs enable efficient hole transfer, reduce charge recombination and minimize energy loss. As a result, we report 15.01% and 11.02% efficiencies for CsPbI2Br and CsPbIBr2 C-IPSCs, respectively, with a high open-circuit voltage of â¼1.3 V. Unencapsulated interface-modified CsPbI2Br devices maintained 92.8% of their initial efficiency at ambient conditions after nearly 2,000 h; and 94.6% after heating at 60 °C for 170 h. This strategy to tailor carbon interface with perovskite offers an important knob in improving C-IPSCs performance.
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Mycobacterium tuberculosis is a pathogenic bacterium that is parasitic in macrophages and show high adaptation to the host's immune response. It can also trigger a complex immune response in the host. This relies on proteins encoded by a series of M. tuberculosis-encoded virulence genes. We found that the M. tuberculosis Rv3435c gene is highly conserved among pathogenic mycobacteria, and might be a virulence gene. To explore the gene function of Rv3435c, we used Mycobacterium smegmatis to construct a recombinant mycobacterium expressing Rv3435c heterologously. The results that Rv3435c is a cell wall-related protein that changes bacterial and colony morphology, inhibits the growth rate of recombinant mycobacteria, and enhances their resistance to various stresses. We also found that the fatty acid levels of the recombinant strain changed. Simultaneously, Rv3435c can inhibit the expression and secretion of inflammatory factors and host cell apoptosis, and enhance the survival of recombinant bacteria in macrophages. Experimental data indicated that Rv3435c might play an important role in Mycobacterium tuberculosis infection.