RESUMO
INTRODUCTION: Fluid resuscitation has long been a cornerstone of pre-hospital trauma care, yet its optimal approach remains undetermined. Although a liberal approach to fluid resuscitation has been linked with increased complications, the potential survival benefits of a restrictive approach in blunt trauma patients have not been definitively established. Consequently, equipoise persists regarding the optimal fluid resuscitation strategy in this population. METHODS: We analysed data from the two largest European trauma registries, the UK Trauma Audit and Research Network (TARN) and the German TraumaRegister DGU® (TR-DGU), between 2004 and 2018. All adult blunt trauma patients with an Injury Severity Score > 15 were included. We examined annual trends in pre-hospital fluid resuscitation, admission coagulation function, and mortality rates. RESULTS: Over the 15-year study period, data from 68,510 patients in the TARN cohort and 82,551 patients in the TR-DGU cohort were analysed. In the TARN cohort, 3.4% patients received pre-hospital crystalloid fluids, with a median volume of 25 ml (20-36 ml) administered. Conversely, in the TR-DGU cohort, 91.1% patients received pre-hospital crystalloid fluids, with a median volume of 756 ml (750-912 ml) administered. Notably, both cohorts demonstrated a consistent year-on-year decrease in the volume of pre-hospital fluid administered, accompanied by improvements in admission coagulation function and reduced mortality rates. CONCLUSION: Considerable variability exists in pre-hospital fluid resuscitation strategies for blunt trauma patients. Our data suggest a trend towards reduced pre-hospital fluid administration over time. This trend appears to be associated with improved coagulation function and decreased mortality rates. However, we acknowledge that these outcomes are influenced by multiple factors, including other improvements in pre-hospital care over time. Future research should aim to identify which trauma populations may benefit, be harmed, or remain unaffected by different pre-hospital fluid resuscitation strategies.
Assuntos
Traumatismo Múltiplo , Ferimentos não Penetrantes , Adulto , Humanos , Estudos Retrospectivos , Ferimentos não Penetrantes/terapia , Escala de Gravidade do Ferimento , Soluções Cristaloides , Hospitais , Sistema de Registros , Alemanha/epidemiologia , Traumatismo Múltiplo/complicaçõesRESUMO
BACKGROUND: The only accepted way to avoid transfusion-associated graft-versus-host disease is irradiation of blood components. With respect to irradiation and associated storage time, different recommendations exist. We examined early irradiated (day +3) leucoreduced red blood cell units for irradiation-associated damages during storage. STUDY DESIGN AND METHODS: We studied 80 leucoreduced units from two manufacturers. All RBCs were stored in the additive solution saline-adenine-glucose-mannitol (SAG-M) and leucoreduced on collection day. Forty components were irradiated on day +3 with 30 Gy, 40 served as non-irradiated controls. Samples were drawn and analysed from these 80 units on days +3, +7, +14, +21, +28, +35 and +42. RESULTS: From day +14, there was a significant difference in the in vitro haemolysis rate between the non-irradiated and the irradiated components. Two of the irradiated units showed a haemolysis rate over the recommended limit of 0·8% on day +42. Potassium and lactate dehydrogenase activity increased faster in irradiated groups during storage. CONCLUSIONS: Our findings show that leucoreduced RBCs which are irradiated early after collection show an obvious radiation damage over storage period. Interestingly, on days +28, +35 and +42 the quality of RBCs which were irradiated on day +3 was nearly identical to the quality of RBCs which were irradiated on day +14 in a former series. Early irradiation does not cause more damage of RBCs during subsequent storage than irradiation on day +14. The maximum storage period of irradiated RBCs should remain restricted to 28 days from collection, however independently from the day of irradiation within this period.
Assuntos
Preservação de Sangue/métodos , Eritrócitos/efeitos da radiação , Crioprotetores/farmacologia , Eritrócitos/citologia , Eritrócitos/metabolismo , Raios gama , Hemólise/efeitos dos fármacos , Hemólise/efeitos da radiação , Humanos , Lactato Desidrogenases/metabolismo , Potássio/análiseRESUMO
BACKGROUND AND OBJECTIVES: Regulatory T cells (Tregs) and other T-cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic setting. MATERIAL AND METHODS: We characterized 53 donors, patients and peripheral blood stem cell concentrates (PBSC) regarding the content of CD45(+) cells, lymphocytes, CD3(+) cells, CD3(+) CD4(+) T cells, CD3(+) CD4(+) CD25(+) T cells, CD3(+) CD4(+) CD25(+) CD127(low/negative) Tregs and CD34(+) cells and calculated cell yields, recruitment factors and collection efficiency for all cell types. We compared allogeneic data with autologous data. RESULTS: Autologous PBSC show significantly lower concentrations of T-cell subsets compared to allogeneic PBSC (17,112/µl CD4(+), 14,858/µl CD4(+) CD25(+) and 1579/µl CD3(+) CD4(+) CD25(+) CD127(low/negative) Tregs in autologous compared to 65,539/µl CD4(+), 44,208(+) /µl CD4(+) CD25(+) and 5040/µl CD3(+) CD4(+) CD25(+) CD127(low/negative) Tregs in allogeneic PBSC, respectively), in contrast to CD34(+) concentrations (5342/µl CD34(+) in autologous compared to 2367/µl CD34(+) in allogeneic PBSC, respectively). Accordantly, all T-cell yields are lower in the autologous setting compared to allogeneic PBSC. However, recruitment factor and collection efficiency of all cell types are higher in autologous compared to allogeneic PBSC, but not all parameters differ significantly when groups are compared. CONCLUSION: T-cell subsets and especially Tregs are a substantial part of PBSC transplantation, as considerable recruitment during apheresis occurs. In large volume apheresis, the collection efficiency of Treg is comparable to that of CD34(+) cells, while recruitment factors are even higher.
Assuntos
Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Células-Tronco Hematopoéticas/imunologia , Linfócitos T Reguladores/imunologia , Adolescente , Adulto , Idoso , Transfusão de Sangue Autóloga/efeitos adversos , Pré-Escolar , Feminino , Transplante de Células-Tronco Hematopoéticas/métodos , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Linfócitos T Reguladores/transplante , Transplante Homólogo/efeitos adversosRESUMO
ThromboLUX (TLX)-Score was compared with hypotonic shock response (HSR) and extent of shape change (ESC) in 99 samples from 42 platelet concentrates. Tests were performed in parallel and duplicate. Mean values for TLX Score, HSR and ESC were 30.3 ± 3.8%, 69.0 ± 12.2% and 23.2 ± 4.9%, respectively. We found no significant correlation between TLX Score and HSR or ESC (r = -0.158, P = 0.118 and r = -115, P = 0.255, respectively), whereas HSR and ESC correlated significantly (r = 0.351, P < 0.001). As TLX Score did not show significant correlation with HSR and ESC, the value of TLX for platelet quality testing remains unclear. Studies comparing these parameters with transfusion outcome are needed.
Assuntos
Plaquetas/fisiologia , Preservação de Sangue/métodos , Pressão Osmótica , Estresse Fisiológico , Plaquetas/citologia , Preservação de Sangue/efeitos adversos , Forma Celular , HumanosRESUMO
The novel allele HLA-B*08:113 identified in two related individuals of Caucasian origin is described.
Assuntos
Alelos , Doadores de Sangue , Sangue Fetal/metabolismo , Antígenos HLA-B/genética , Sequência de Bases , Éxons/genética , Feminino , Alemanha , Humanos , Masculino , Dados de Sequência Molecular , Mães , Alinhamento de SequênciaRESUMO
BACKGROUND AND OBJECTIVES: Since 2001, all blood components in Germany must be leucocyte depleted. Recently, a new method for quality control of depletion was introduced. Our study aimed at the validation of the method for routine use in apheresis platelet concentrates. MATERIALS AND METHODS: We compared the new ADAM-rWBC device with manual counting in the Nageotte chamber and flow cytometry, two standard methods, by measuring residual leucocytes in 40 units of apheresis platelet concentrates and in six geometrical dilution series. RESULTS: Cell counts of residual leucocytes in the 40 units were below 10(6) cells per component with all methods, although mean cell counts were approximately 5 and 6 times higher in flow cytometry and ADAM-rWBC, respectively, compared to the Nageotte chamber. No unit with <10(6) leucocytes was regarded as contaminated. The dilution series showed acceptable accuracy, especially in the range around the cut-off (approximately 4·5 cells/µl in components with a volume of 220 ml) for regarding a concentrate as contaminated with leucocytes. No sample spiked with more than 4·5 cells/µl was counted as having less. CONCLUSION: In comparison with manual counting and flow cytometry, the ADAM-rWBC device performed equally. The method is suitable for routine screening of leucocyte contamination of apheresis platelets.
Assuntos
Citometria de Fluxo/métodos , Contagem de Leucócitos , Microscopia/instrumentação , Plaquetoferese , Transfusão de Componentes Sanguíneos , Humanos , Controle de Qualidade , Reprodutibilidade dos TestesRESUMO
BACKGROUND: Uveal melanoma is the most common primary intraocular malignancy in adults. Iris melanomas are rare tumours: they account for 2-3â% of all uveal melanomas. The clinical differentiation between benign iris nevi and malignant iris melanomas can be difficult. PATIENTS AND METHODS: The aim of this study was the registration, analysis and observation of all patients with tumours of the anterior uvea who had been treated surgically between 1992 and 2011 at the ophthalmic department of the University Hospital of Jena. 40 patients were analysed and compared concerning their preoperative states, operating methods including complications, histological results, postoperative function, subjective complaints as well as the risk of metastasis and the associated dependence on mortality of the dignity of the tumours. In this time period 26 patients has been observed in a follow-up visit. Patients with a malignant tumour were offered an examination. RESULTS: The histological examination revealed for 24 patients a benign tumour and for 16 patients a malignant tumour. After an exact analysis of multiple parameters there was only a statistically significant difference in the preoperative visual acuity (p = 0.025) and in the tumour size (p = 0.011) between the two analysed groups of patients. The rate of serious postoperative complications was 11.4â%. One fourth of the patients complained of subjective problems after the surgical intervention. In the follow-up visit a visual acuity of 0.5 or better was achieved in 68â% of all interventions. CONCLUSIONS: A reliable diagnosis is only possible after histological examination. The analysed parameters can only give indications for the dignity of the tumour. The strategy of the ophthalmic department of the University Hospital of Jena to remove a tumour of uncertain dignity at an early state makes sense, because there are few postoperative complications, few patients complain about subjective problems and the chances for achieving good visual acuity are high.
Assuntos
Iridectomia , Neoplasias da Íris/diagnóstico , Neoplasias da Íris/cirurgia , Melanoma/diagnóstico , Melanoma/cirurgia , Transtornos da Visão/prevenção & controle , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Alemanha , Humanos , Neoplasias da Íris/patologia , Estudos Longitudinais , Masculino , Melanoma/complicações , Melanoma/patologia , Pessoa de Meia-Idade , Resultado do Tratamento , Transtornos da Visão/diagnóstico , Transtornos da Visão/etiologia , Adulto JovemRESUMO
BACKGROUND AND OBJECTIVES: Blood gas analysers measuring pH at 37°C (pH37) are widely used for pH determination of platelet (PLT) concentrates (PCs). For reporting pH at 22°C (pH22), converting of pH37 using the correct conversion factor is mandatory. For PCs stored in PLT additive solution (PAS), such conversion factors are not yet widely available. We studied pH in samples of PCs with different PAS/plasma ratios during warming from 22 to 37°C. MATERIALS AND METHODS: We measured pH in 39 samples containing modified PAS-III (PAS-IIIM) with a plasma carryover of 20%, 30% or 40% or no PAS-IIIM. Differences between pH22 and pH37 (dpH) were compared within and between study groups. Correlation between pH22 and dpH was tested. Additional measurements in 33 samples with three different PLT counts were performed to study the influence of PLT count on dpH. RESULTS: pH22 and pH37 within each group and dpH or dpH/dT between study groups differed significantly. The dpH was 0·135 ± 0·040, 0·021 ± 0·009, 0·033 ± 0·011 and 0·048 ± 0·017 for samples containing 100%, 20%, 30% or 40% plasma, respectively. Correlation between dpH and pH22 was strong in 100% (r = 0·696, P < 0·001), weaker in 30% and 40% (r = 0·367, P = 0·022 and r = 0·345, P = 0·032, respectively) and not existing in 20% plasma (r = 0·153, P = 0·354). PLT count did not influence the dpH significantly. CONCLUSION: The dpH is dependent on different PAS-IIIM/plasma ratios and pH range. For precise reporting of pH22, the respective dpH must be used if converting is necessary. Preferably, the pH should be reported at 37°C or measured directly at 22°C.
Assuntos
Acetatos/sangue , Análise Química do Sangue/métodos , Plaquetas/química , Plaquetas/metabolismo , Citratos/sangue , Cloreto de Sódio/sangue , Acetatos/química , Preservação de Sangue , Citratos/química , Humanos , Concentração de Íons de Hidrogênio , Cloreto de Sódio/química , TemperaturaRESUMO
BACKGROUND: There is a lack of data on the usage of plasma derived/recombinant coagulation factor concentrates (PD/RCFC) regarding diagnostic categories. An audit of PD/RCFC and blood component usage at a tertiary care teaching hospital in northern Bavaria was conducted. STUDY DESIGN AND METHODS: All blood components and PD/RCFC transfused at a university hospital (Erlangen, Germany) during the year 2006 were analysed. Transfused blood products were listed by major diagnostic categories (MDC) formed from principal diagnoses of recipients according to the International Classification of Diseases, tenth revision and German modification. RESULTS: Blood component usage has markedly increased since last surveyed in 1994 through 1996. The diagnostic categories responsible for most transfusions have not changed since. Antithrombin is the PD/RCFC used most, whereas most money for PD/RCFC was spent on FVIII concentrates. Polytrauma patients need most fibrinogen, whereas most of FXIII is needed in patients with malignancies. Patients with prolonged artificial ventilation receive PCC most often. Altogether, three MDCs (Pre, 17, 05) accounted for 80·5% of costs created by PD/RCFC transfusion. CONCLUSION: This study provides for the first time combined data on blood component and PD/RCFC usage in a German university hospital. It shows that the MDCs responsible for most of the costs in transfusion therapy with blood components and with PD/RCFC are few, and are the same. At the same time, blood bank information software should be further improved in order to be able to identify new trends in hemotherapy in more detail.
Assuntos
Fatores de Coagulação Sanguínea , Hospitais de Ensino , Auditoria Médica , Proteínas Recombinantes , Alemanha , HumanosRESUMO
BACKGROUND: In a prospective clinical study we examined the course of the optical density of the macular pigment (MPOD) in patients with idiopathic macular holes before and after pars-plana vitrectomy. PATIENTS AND METHODS: Of the 26 patients four had macular holes stage 1, six patients had stage 2 or stage 4 holes and ten people had a macular hole stage 3. The surgical procedure consisted always of a standard 3-port pars plana vitrectomy (PPV) with colouring assisted peeling of the internal limiting membrane. The optical density of macular pigment was determined by the 1-wavelength reflection method before pars plana vitrectomy and after macular hole closure. RESULTS: The macular hole was completely closed in 25 of 26 eyes (96.15â%). This anatomical success is also the basis for a postoperative improvement in the retinal function. In the case of macular holes from stages 3 and 4 the surgery achieved an increase in visual acuity and an increase of the optical density of the the macular pigment. In patients with macular holes stage 2 the visual acuity and the optical density of the macular pigment were reduced after vitrectomy. In the case of stage 1 holes the visual acuity and the volume of macular pigment also were reduced after the operation. DISCUSSION: In the group of stage 4 macular holes there was a significant increase in maximum optical density and volume of macular pigment after successful surgical hole closure, so the functional profit of PPV is very high in these patients. Even in the case of macular holes of stage 3 the PPV effected a functional improvement in the sense of an increase of the optical density of macular pigment. The increase in volume proved to be significant. For macular holes stage 2 the vitrectomy as a therapy option is generally recognised but PPV does not effect positively the height of the optical density of macular pigment. In these patients both the maximum optical density as well as the volume of macular pigment were reduced in the therapeutic course. Whether surgical intervention is indicated in stage 1 is still controversial. Taking into account the development of MPOD the profit from surgery for the patients is very low. This led to a decrease in volume of macular pigment and only a slight increase in maximum optical density after pars plana vitrectomy.
Assuntos
Densitometria , Membrana Epirretiniana/patologia , Membrana Epirretiniana/cirurgia , Complicações Pós-Operatórias/diagnóstico , Perfurações Retinianas/diagnóstico , Perfurações Retinianas/cirurgia , Vitrectomia/métodos , Idoso , Feminino , Angiofluoresceinografia , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Oftalmoscopia , Complicações Pós-Operatórias/fisiopatologia , Estudos Prospectivos , Perfurações Retinianas/classificação , Tomografia de Coerência Óptica , Acuidade Visual/fisiologiaRESUMO
The novel allele HLA-B*07:68:02 identified in two related individuals of Caucasian origin is described.
Assuntos
Sangue Fetal/imunologia , Antígenos HLA-B/genética , Teste de Histocompatibilidade/métodos , Alelos , Sequência de Bases , Doadores de Sangue , Éxons , Pai , Feminino , Alemanha , Antígeno HLA-B7 , Humanos , Imunogenética , Masculino , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Polimorfismo GenéticoRESUMO
BACKGROUND AND OBJECTIVES: Recently, it was reported that leucocytes obtained from leucoreduction system chambers (LRSCs) after plateletapheresis show excellent quality due to culture of dendritic cells. This study analysed apoptosis of mononuclear cells derived from LRSCs of single platelet units (SPUs) and double platelet units (DPUs) during storage. MATERIALS AND METHODS: This randomized prospective study compared eighteen single and double platelet units produced with the Trima Accel cell separator. Buffy coat was drained from the LRSCs and analysed after 1, 6, 24, 48 and 72 h. CD45+ lymphocytes and CD14+ monocytes cells as well as Annexin-V+ and 7-AAD+ mononuclear cells were measured by flow cytometry. RESULTS: The WBC concentration of LRSCs obtained from SPUs and DPUs differed significantly (SPUs: 0·93 ± 0·32 ×10(5) per µl WBCs; DPUs: 1·71 ± 0·55 ×10(5) per µl WBCs; P<0·001). Processed blood volume (PBV) correlated significantly with WBC concentration (r(2)=0·75, P<0·001). Fifty percent of monocytes were Annexin-V-positive 1 h after production decreasing to 30% during 24 h of storage. Compared to that, the part of late apoptotic or necrotic PBMCs increased later on, after 24 h. After 24 h, Annexin-V- and 7-AAD-positive, late apoptotic and necrotic lymphocytes and monocytes doubled. CONCLUSION: PBMCs stored in autologous plasma in PVC-bags at room temperature did not show an increase of 7-AAD-positive PBMCs during 24 h prior to cell processing but increased significantly thereafter.
Assuntos
Apoptose/fisiologia , Preservação de Sangue/métodos , Procedimentos de Redução de Leucócitos/métodos , Leucócitos Mononucleares/citologia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Plaquetoferese/métodos , Estudos Prospectivos , Adulto JovemRESUMO
BACKGROUND: There exists only few data on in vitro and in vivo effects of gamma irradiation of leucoreduced red blood cells (RBCs). Reported studies reflect the effects of early irradiation and subsequent storage. The effects of irradiation on RBCs shortly before the end of their shelf-life have not been examined. STUDY DESIGN AND METHODS: We studied 160 RBC units that were stored in the additive solution saline-adenine-glucose-mannitol and leucoreduced on the collection day. Forty components were irradiated on day +14 with 30 Gy, 40 on day +28, 40 on day +35, and 40 served as nonirradiated controls. In vitro evaluation of all units was performed on days +3, +7, +14, +21, +28, +35, and +42 from the collection day. RESULTS: Gamma irradiation induced leakage of potassium ions and lactate dehydrogenase and enhanced in vitro haemolysis rate in the irradiated components, which started to increase faster than that of nonirradiated RBCs from the day of irradiation, i.e. from day +14 in units that were irradiated on day +14, from day +28 in units that were irradiated on day +28, and from day +35 in units that were irradiated on day +35. CONCLUSIONS: This study presents data on the in vitro quality of leucoreduced RBCs that have been irradiated on days +14, +28, or +35 after collection. Our findings support the proposal that the current limitation of the age of RBCs on the day of gamma irradiation may be replaced by staged limitations depending on the time of irradiation.
Assuntos
Preservação de Sangue/métodos , Eritrócitos/efeitos da radiação , Raios gama , Adenina , Glucose , Hemólise/efeitos da radiação , Humanos , Procedimentos de Redução de Leucócitos , Manitol , Permeabilidade/efeitos da radiação , Cloreto de Sódio , Fatores de TempoRESUMO
Black cohosh is one of the most popular herbal therapies for premenstrual discomfort, hot flushes and other climacteric and menopausal symptoms. Most often, it is tolerated well. However, there are some recent reports on serious adverse events, probably associated with this complementary and alternative herbal medicine. We report a case of coagulation activation, fluid retention and transient autoimmune hepatitis most likely triggered by the use of black cohosh. Diagnostic procedures aimed to explain lower leg edema are not uncommon in the age group of women suffering from climacteric and menopausal symptoms. Therefore, black cohosh-induced fluid retention and coagulation activation should be considered in differential diagnosis, especially if thrombosis has been excluded.
Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Cimicifuga/efeitos adversos , Edema/induzido quimicamente , Fitoterapia , Extratos Vegetais/efeitos adversos , Síndrome Pré-Menstrual/tratamento farmacológico , Adulto , Cimicifuga/química , Edema/patologia , Feminino , Hepatite Autoimune/etiologia , Humanos , Fígado/enzimologia , Extratos Vegetais/uso terapêuticoRESUMO
OBJECTIVE: Purpose of these study was the evaluation of a new method for the measurement of haemoglobin oxygenation in retinal vessels. Patients with branch retinal artery occlusion have been measured before and after a rheological therapy. METHODS: The haemoglobin oxygenation in retinal vessels was measured by the "oxygen module" (Imedos GmbH, Jena, Germany) which uses a special filter (transmission at 548 and 610 nm, band width 10 nm) in the illumination path of a fundus camera. Fundus images were acquired by a colour CCD camera and specific software was used for vessel tracking and calculation of their haemoglobin oxygen saturation. 11 patients with branch retinal artery occlusions, average age 63.4 ± 11.7 years were investigated at time of diagnosis and at the 5th day of a rheological and intraocular pressure reduction therapy. RESULTS: 10 out of 11 patients have shown an increase of haemoglobin oxygenation over time (on average from 73 ± 16 % to 87 ± 11 %). This increase was statistically significant (p < 0.05, Wilcoxon test). Non-affected retinal areas have also shown an increase of haemoglobin oxygenation. The visual acuity improved by one line. This increase in visual acuity was, however, not statistically significant. CONCLUSIONS: We observed an increase of the arterial haemoglobin oxygenation. One possible reason of the improvement of the haemoglobin oxygenation by the therapy may be an increase of the rheological properties of the blood. All patients showed an increase or at least a stabilisation of their visual acuity. Retinal vessel oximetry might be used as an additional parameter in the follow-up of retinal artery occlusion.
Assuntos
Oximetria/instrumentação , Oxiemoglobinas/análise , Oclusão da Artéria Retiniana/sangue , Oclusão da Artéria Retiniana/diagnóstico , Artéria Retiniana/metabolismo , Biomarcadores/sangue , Desenho de Equipamento , Análise de Falha de Equipamento , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Estatística como Assunto , Resultado do TratamentoRESUMO
OBJECTIVES: Oxygen saturation in retinal vessels can be measured by spectrometry. However, there are no studies on the influence of different camera chips on these measurements. The presented study reports the effect of two different camera chips ("1-CCD" vs. "3-CCD") on the spectrometric measurements in the same patients. METHODS: 61 eyes of 61 patients were included in this study. The study was approved by the local ethics commission. Patients were separated in 3 groups: the first group contained healthy volunteers, the second group patients with diabetes mellitus but without previous laser treatment, the third group included patients with diabetes mellitus and previous laser coagulation treatment. The oxygen saturation was calculated from the difference between wavelengths on and right next to the retinal vessel. This methodology uses the different absorption spectra of oxy- and deoxyhaemoglobin. RESULTS: All 3 groups demonstrated valid outcomes of the retinal oxygen saturation in arterial and venous vessels. No significant differences we found in the arterial-venous oxygen difference between the two different camera chips (group 1 p = 0.063, group 2 p = 0.204, group 3 p = 0.059). CONCLUSION: As no significant differences could be found between the two camera chips, the less expensive system should be used if the spectrometric measurement of oxygen saturation is added to an analysis system already established on the market.
Assuntos
Retinopatia Diabética/diagnóstico , Retinopatia Diabética/metabolismo , Oximetria/instrumentação , Oxigênio/análise , Processamento de Sinais Assistido por Computador/instrumentação , Análise Espectral/instrumentação , Retinopatia Diabética/cirurgia , Desenho de Equipamento , Análise de Falha de Equipamento , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Oximetria/métodos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , TransdutoresAssuntos
Transplante de Células-Tronco de Sangue do Cordão Umbilical , Éxons/genética , Cadeias beta de HLA-DQ/genética , Sequência de Bases , Feminino , Alemanha , Teste de Histocompatibilidade , Humanos , Masculino , Dados de Sequência Molecular , Polimorfismo de Nucleotídeo Único , Alinhamento de Sequência , Doadores de Tecidos , População BrancaRESUMO
HLA-A*01:234 was identified by next-generation sequencing and confirmed by Sanger sequencing.
Assuntos
Sangue Fetal/fisiologia , Antígeno HLA-A1/genética , Alelos , Doadores de Sangue , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Teste de Histocompatibilidade , Humanos , MãesRESUMO
HLA-C*01:136 identified by next generation sequencing and confirmed by Sanger sequencing.
Assuntos
Alelos , Doadores de Sangue , Sangue Fetal/metabolismo , Antígenos HLA-C/genética , Azerbaijão , Sequência de Bases , Éxons/genética , Humanos , Alinhamento de SequênciaRESUMO
PURPOSE: To examine anterior chamber depth (ACD) and anterior chamber angle (ACA) in patients with glaucoma after cataract extraction with an anterior optical coherence tomography (OCT) system. METHODS: A new commercially available 1310 nm infrared light anterior segment OCT system was used for anterior chamber evaluation. Sixty patients (n=20 with primary open angle glaucoma [POAG], n=20 with angle closure glaucoma [ACG], and n=20 with no known glaucoma as control group) with a mean age of 68.8+/-13.9 years undergoing cataract surgery were enrolled. RESULTS: Before cataract surgery, ACD and ACA of the ACG group were significantly lower compared to the POAG and control groups (ACD p<0.005; ACA p<0.005). After cataract extraction, ACD and ACA increased significantly in the ACG group (3.1+/-0.4 mm vs 1.8+/-0.2 mm, p<0.005 and 32.3 degrees +/-7.7 degrees vs 16.0 degrees +/-4.7 degrees , p<0.005). In the POAG and control groups, ACD and ACA also increased postoperatively, but not as much as in the ACG group. After cataract extraction, IOP decreased significantly in the glaucoma groups (ACG: 15.6+/-6.1 vs 18.6+/-5.7 mm Hg, p=0.008; POAG: 16.2+/-3.4 vs 20.7+/-8.0 mmHg, p=0.02) and was almost the same in all three groups after surgery (control group after surgery: 15.2+/-2.8 mmHg). CONCLUSIONS: Both ACD and ACA increased significantly after cataract extraction and IOL implantation. IOP reduction after surgery was higher in the glaucoma groups compared to the control group.