RESUMO
The fabrication of electroactive nanostructures with high electron concentration and specific electron transport is crucial for electrochemical sensing. In this study, a plasmon-enhanced electrochemical sensor has been developed for the detection of extracellular hydrogen peroxide (H2O2) from cancer cells, utilizing Pd-tipped Au nanobipyramids (PTA NBPs) as the electrocatalysts. Plasmonic PTA NBPs were synthesized by depositing Pd nanoparticles onto the tips of Au nanobipyramids (Au NBPs). Under excitation of localized surface plasmon resonance (LSPR), the PTA NBPs generate high-energy electron-hole pairs (e-/h+) on their surface. The generated electrons (e-) significantly enhance the electrochemical reduction of H2O2. Based on this, a plasmon-enhanced H2O2 electrochemical sensor is constructed with high sensitivity (986.57 µA mM-1 cm-2), low detection limit (0.02 µM), wide linear range (0.1 µM to 980 µM), and good stability and repeatability. Moreover, this sensor also enables the measurement of extracellular H2O2 derived from cancer cells (MCF-7), highlighting its potential applications in cellular biology and biomedical research.
Assuntos
Nanopartículas Metálicas , Nanoestruturas , Peróxido de Hidrogênio/química , Nanopartículas Metálicas/química , Ouro/química , Ressonância de Plasmônio de Superfície , Técnicas EletroquímicasRESUMO
A cascade 6-endo-dig cyclization reaction was developed for the switchable synthesis of halogen and non-halogen-functionalized pyrazolo[3,4-b]pyridines from 5-aminopyrazoles and alkynyl aldehydes via C≡C bond activation with silver, iodine, or NBS. In addition to its wide substrate scope, the reaction showed good functional group tolerance as well as excellent regional selectivity. This new protocol manipulated three natural products, and the arylation, alkynylation, alkenylation, and selenization of iodine-functionalized products. These reactions demonstrated the potential applications of this new method.