Electrochemical promotion of organic waste fermentation: Research advances and prospects.

The current methods of treating organic waste suffer from limited resource usage and low product value. Research and development of value-added products emerges as an unavoidable trend for future growth. Electro-fermentation (EF) is a technique employed to stimulate cell proliferation, expedite microbial metabolism, and enhance the production of value-added products by administering minute voltages or currents in the fermentation system. This method represents a novel research direction lying at the crossroads of electrochemistry and biology. This article documents the current progress of EF for a range of value-added products, including gaseous fuels, organic acids, and other organics. It also presents novel value-added products, such as 1,3-propanediol, 3-hydroxypropionic acid, succinic acid, acrylic acid, and lysine. The latest research trends suggest a focus on EF for cogeneration of value-added products, studying microbial community structure and electroactive bacteria, exploring electron transfer mechanisms in EF systems, developing effective methods for nutrient recovery of nitrogen and phosphorus, optimizing EF conditions, and utilizing biosensors and artificial neural networks in this area. In this paper, an analysis is conducted on the challenges that currently exist regarding the selection of conductive materials, optimization of electrode materials, and development of bioelectrochemical system (BES) coupling processes in EF systems. The aim is to provide a reference for the development of more efficient, advanced, and value-added EF technologies. Overall, this paper aims to provide references and ideas for the development of more efficient and advanced EF technology.

Exploring the maximum nitrite production rate through the granular sludge-type reactor to match the needs of anammox process realizing efficient nitrogen removal.

The reliable and efficient nitrite production rate (NPR) through nitritation process is the prerequisite for the efficient running of subsequent processes, like the anammox process and the nitrite shunt. However, there has been scant research on stable and productive nitritation process in recent years. In this study, at a stable hydraulic retention time of 12.0 h and with precise and strict DO control, the upper limit of the NPR was initially investigated using a continuous-flow granular sludge reactor. The NPR of 1.69 kg/m3/d with a nitrite production efficiency of 81.97% was finally achieved, which set a record until now in similar research. The median sludge particle size of 270.0 µm confirmed the development of clearly defined granular sludge. The genus Nitrosomonas was the major ammonium oxidizing bacteria. In conclusion, this study provides valuable insights for the practical application of the effective nitritation process driving subsequent nitrogen removal processes.

From waste to wealth: Innovations in organic solid waste composting.

Organic solid waste (OSW) is not only a major source of environmental contamination, but also a vast store of useful materials due to its high concentration of biodegradable components that can be recycled. Composting has been proposed as an effective strategy for recycling OSW back into the soil in light of the necessity of a sustainable and circular economy. In addition, unconventional composting methods such as membrane-covered aerobic composting and vermicomposting have been reported more effective than traditional composting in improving soil biodiversity and promoting plant growth. This review investigates the current advancements and potential trends of using widely available OSW to produce fertilizers. At the same time, this review highlights the crucial role of additives such as microbial agents and biochar in the control of harmful substances in composting. Composting of OSW should include a complete strategy and a methodical way of thinking that can allow product development and decision optimization through interdisciplinary integration and data-driven methodologies. Future research will likely concentrate on the potential in controlling emerging pollutants, evolution of microbial communities, biochemical composition conversion, and the micro properties of different gases and membranes. Additionally, screening of functional bacteria with stable performance and exploration of advanced analytical methods for compost products are important for understanding the intrinsic mechanisms of pollutant degradation.

Bioconversion of biowaste into renewable energy and resources: A sustainable strategy.

Due to its high amount of organic and biodegradable components that can be recycled, biowaste is not only a major cause of environmental contamination, but also a vast store of useful materials. The transformation of biowaste into energy and resources via biorefinery is an unavoidable trend, which could aid in reducing carbon emissions and alleviating the energy crisis in light of dwindling energy supplies and mounting environmental difficulties related with solid waste. In addition, the current pandemic and the difficult worldwide situation, with their effects on the economic, social, and environmental aspects of human life, have offered an opportunity to promote the transition to greener energy and sources. In this context, the current advancements and possible trends of utilizing widely available biowaste to produce key biofuels (such as biogas and biodiesel) and resources (such as organic acid, biodegradable plastic, protein product, biopesticide, bioflocculant, and compost) are studied in this review. To achieve the goal of circular bioeconomy, it is necessary to turn biowaste into high-value energy and resources utilizing biological processes. In addition, the usage of recycling technologies and the incorporation of bioconversion to enhance process performance are analyzed critically. Lastly, this work seeks to reduce a number of enduring obstacles to the recycling of biowaste for future use in the circular economy. Although it could alleviate the global energy issue, additional study, market analysis, and finance are necessary to commercialize alternative products and promote their future use. Utilization of biowaste should incorporate a comprehensive approach and a methodical style of thinking, which can facilitate product enhancement and decision optimization through multidisciplinary integration and data-driven techniques.

A state-of-the-art review of CO2 enhanced oil recovery as a promising technology to achieve carbon neutrality in China.

Although there are some review papers on carbon capture, utilization and storage (CCUS), hardly any of these reviews are focused on the role of CO2 enhanced oil recovery (EOR) in accelerating carbon neutrality in China. In this review, strategies to achieve carbon neutrality is briefly but critically discussed, followed by a review of CO2-EOR as a promising technology. Especially, data analysis, including the number of publications on China's carbon neutrality, per capita CO2 emissions, China's power generation, and the crude oil production of China's large oilfields, is carried out to make the discussion more comprehensive. Given the large amount of coal consumed in China, the high percent of electricity generated with coal, and the slow penetration of renewables already observed, it seems unlikely that 2060 targets will be met without CCUS. In order to achieve carbon neutrality, both reduction in carbon emissions and increase in carbon sequestration are inevitable. Furthermore, it is concluded that CO2 storage through EOR is likely to have a bright future. However, there are some critical issues to be solved, including the technical issues, leakage and safety issues, cost issues, policy issues, etc. In order to turn CO2-EOR into a reliable and more favorable technology, more research and efforts are needed to solve these issues, including advancing carbon capture technologies, improving storage technologies, developing effective monitoring technologies, deploying government support and incentive policies, etc.

Impact of influent strengths on nitrous oxide emission and its molecular mechanism in constructed wetlands treating swine wastewater.

Constructed wetlands (CWs) can remove nitrogen (N) through plant assimilation and microbial nitrification and denitrification, while it also releases large greenhouse gas nitrous oxide (N2O) into the atmosphere. However, N2O emissions and the underlying microbial mechanisms of CWs when treating high-strength wastewater have not been systematically surveyed. Here, the effect of three influent strengths on N2O emissions in a pilot-scale CW treatment of swine wastewater was determined and the underlying microbial mechanisms were explored. The results showed that the removal rates of ammonium (NH4+) and total nitrogen (TN) increased significantly with the increasing influent strengths, however, the ratio of N2O emission/TN removal rose by 1.5 times at the same time. Quantitation of microorganisms responsible for N-cycle in the sediment indicated that the abundance of ammonia-oxidizing bacteria (AOB) in high influent strengths (COD, 962.38 ± 3.05 mg/L; NH4+, 317.89 ± 4.24 mg/L) was 51.6-fold compared with that in low influent strengths (COD, 516.94 ± 4.18 mg/L; NH4+, 100.65 ± 2.65), and AOB gradually replaced ammonia-oxidizing archaea (AOA) to dominate ammonia oxidizers. Structural equation models demonstrated that NO2- accumulations promoted the ratio of AOB/AOA, which further led to an increase in the ratio of N2O emission/TN removal. It is worth noting both the N removal rates and N2O emissions increased with the increasing influent strength. To obtain reduced N2O emissions, pretreatment technology for strength reduction should be supplemented before high-strength wastewater enters the CWs. This study may shed new light on the sustainable operation and application of CWs.

Transcriptomic Sequencing and Co-Expression Network Analysis on Key Genes and Pathways Regulating Nitrogen Use Efficiency in Myriophyllum aquaticum.

Massively input and accumulated ammonium is one of the main causes of eutrophication in aquatic ecosystems, which severely deteriorates water quality. Previous studies showed that one of the commonly used macrophytes, Myriophyllum aquaticum, was capable of not only withstanding ammonium of high concentration, but also efficiently assimilating extracellular ammonium to constitutive amino acids and proteins. However, the genetic mechanism regulating such efficient nitrogen metabolism in M. aquaticum is still poorly understood. Therefore, RNA-based analysis was performed in this study to understand the ammonium regulatory mechanism in M. aquaticum in response to various concentrations of ammonium. A total of 7721 genes were differentially expressed, of which those related to nitrogen-transport, assimilation, and remobilization were highly-regulated in response to various concentrations of ammonium. We have also identified transcription factors and protein kinases that were rapidly induced in response to ammonium, which suggests their involvement in ammonium-mediated signalling. Meanwhile, secondary metabolism including phenolics and anthocyanins biosynthesis was also activated in response to various concentrations of ammonium, especially at high ammonium concentrations. These results proposed a complex physiological and genetic regulation network related to nitrogen, carbohydrate, transcription factors, and secondary metabolism for nitrogen use efficiency in M. aquaticum.

Enhancement of facultative anaerobic denitrifying communities by oxygen release from roots of the macrophyte in constructed wetlands.

The radial oxygen loss (ROL) of wetland plants is a crucial factor that can influence the efficiency required for nitrogen (N) removal and microbial activities responsible for N removal in constructed wetlands (CWs). However, the shift of microbial community in different niches in response to ROL has been rarely studied. This study aims to unravel the link between the ROL and microbial response in sediment, water and rhizoplane by a surface flow CW planted with Myriophyllum aquaticum for treating high-strength swine wastewater. Ti3+-citrate colorimetric method demonstrated that M. aquaticum was a wetland species with a ROL of 0.019 mg/h/plant. Using quantitative polymerase chain reactions (qPCR) and high-throughput sequencing of microbial 16S rRNA gene, we demonstrated that the abundance of facultative anaerobic denitrifiers in the rhizoplane was the most of the three niches, that in the water (5-10 cm) was the less and that in the sediment was the least. Acinetobacter was enriched and dominated amongst denitrifiers in the water. Denitrifiers in the rhizoplane were mainly dominated by enriched Pseudomonas, Aeromonas, and Acinetobacter. The theoretical calculation of oxygen sources and consumptions indicated that water reaeration should support the oxygen demands for nitrification in the aerobic layer (0-5 cm), and the ROL could stimulate the growth of facultative anaerobic denitrifiers in the rhizoplane and water (5-10 cm) to achieve denitrification within CW systems.

Mitigation of nitrous oxide emissions from acidic soils by Bacillus amyloliquefaciens, a plant growth-promoting bacterium.

Nitrous oxide (N2 O) is a long-lived greenhouse gas that can result in the alteration of atmospheric chemistry and cause accompanying changes in global climate. To date, many techniques have been used to mitigate the emissions of N2 O from agricultural fields, which represent one of the most important sources of N2 O. In this study, we designed a greenhouse pot experiment and a microcosmic serum bottle incubation experiment using acidic soil from a vegetable farm to study the effects of Bacillus amyloliquefaciens (BA) on plant growth and N2 O emission rates. The addition of BA to the soil promoted plant growth enhanced the soil pH and increased the total nitrogen (TN) contents in the plants. At the same time, it decreased the concentrations of ammonium (NH4+ ), nitrate (NO3- ) and TN in the soil. Overall, the addition of BA resulted in a 50% net reduction of N2 O emissions compared with the control. Based on quantitative PCR and the network analysis of DNA sequencing, it was demonstrated that BA partially inhibited the nitrification process through the significant reduction of ammonia oxidizing bacteria. Meanwhile, it enhanced the denitrification process, mainly by increasing the abundance of N2 O-reducing bacteria in the treatment with BA. The results of our microcosm experiment provided evidence that strongly supported the above findings under more strictly controlled laboratory conditions. Taken together, the results of our study evidently demonstrated that BA has dual effects on the promotion of plant growth and the dramatic reduction of greenhouse emissions, thus suggesting the possibility of screening beneficial microbial organisms from the environment that can promote plant growth and mitigate greenhouse trace gases.

Linking N2O Emissions from Biofertilizer-Amended Soil of Tea Plantations to the Abundance and Structure of N2O-Reducing Microbial Communities.

Nitrous oxide (N2O) contributes up to 8% of global greenhouse gas emissions, with approximately 70% from terrestrial sources; over one-third of this terrestrial emission has been linked to increased agricultural fertilizer use. Much of the nitrogen in fertilizers is converted to N2O by microbial processes in soil. However, the potential mechanism of biofertilizers and the role of microbial communities in mitigating soil N2O emissions are not fully understood. Here, we used a greenhouse-based pot experiment with tea plantation soil to investigate the effect of Trichoderma viride biofertilizer on N2O emission. The addition of biofertilizer reduced N2O emissions from fertilized soil by 67.6%. Quantitative PCR (qPCR) analysis of key functional genes involved in N2O generation and reduction ( amoA, nirK, nirS, and nosZ) showed an increased abundance of nirS and nosZ genes linked to the pronounced reduction in N2O emissions. High-throughput sequencing of nosZ showed enhanced relative abundance of nosZ-harboring denitrifiers in the T. viride biofertilizer treatments, thus linking greater N2O reduction capacity to the reduced emissions. Our findings showed that biofertilizers can affect the microbial nitrogen transformation process and reduce N2O emissions from agroecosystems.

Performance and recent improvement in microbial fuel cells for simultaneous carbon and nitrogen removal: A review.

Microbial fuel cells (MFCs) have become a promising technology for wastewater treatment accompanying electricity generation. Carbon and nitrogen removal can be achieved by utilizing the electron transfer between the anode and cathode in an MFC. However, large-scale power production and high removal efficiency must be achieved at a low cost to make MFCs practical and economically competitive in the future. This article reviews the principles, feasibility and bottlenecks of MFCs for simultaneous carbon and nitrogen removal, the recent advances and prospective strategies for performance improvement, as well as the involved microbes and electron transfer mechanisms.

A comprehensive review on microbial lipid production from wastes: research updates and tendencies.

Microbial lipids have recently attracted attention as an intriguing alternative for the biodiesel and oleochemical industries to achieve sustainable energy generation. However, large-scale lipid production remains limited due to the high processing costs. As multiple variables affect lipid synthesis, an up-to-date overview that will benefit researchers studying microbial lipids is necessary. In this review, the most studied keywords from bibliometric studies are first reviewed. Based on the results, the hot topics in the field were identified to be associated with microbiology studies that aim to enhance lipid synthesis and reduce production costs, focusing on the biological and metabolic engineering involved. The research updates and tendencies of microbial lipids were then analyzed in depth. In particular, feedstock and associated microbes, as well as feedstock and corresponding products, were analyzed in detail. Strategies for lipid biomass enhancement were also discussed, including feedstock adoption, value-added product synthesis, selection of oleaginous microbes, cultivation mode optimization, and metabolic engineering strategies. Finally, the environmental implications of microbial lipid production and possible research directions were presented.

A review on three-dimensional electrochemical technology for the antibiotic wastewater treatment.

The potential genotoxicity and non-biodegradability of antibiotics in the natural water bodies threaten the survival of various living things and cause serious environmental pollution and destruction. Three-dimensional (3D) electrochemical technology is considered a powerful means for antibiotic wastewater treatment as it can degrade non-biodegradable organic substances into non-toxic or harmless substances and even completely mineralize them under the action of electric current. Therefore, antibiotic wastewater treatment using 3D electrochemical technology has now become a hot research topic. Thus, in this review, a detailed and comprehensive investigation was conducted on the antibiotic wastewater treatment using 3D electrochemical technology, including the structure of the reactor, electrode materials, the influence of operating parameters, reaction mechanism, and combination with other technologies. Many studies have shown that the materials of electrode, especially particle electrode, have a great effect on the antibiotic wastewater treatment efficiency. The influence of operating parameters such as cell voltage, solution pH, and electrolyte concentration was very significant. Combination with other technologies such as membrane and biological technologies has effectively increased antibiotic removal and mineralization efficiency. In conclusion, the 3D electrochemical technology is considered as a promising technology for the antibiotic wastewater treatment. Finally, the possible research directions of the 3D electrochemical technology for antibiotic wastewater treatment were proposed.

Intermittent energization improves microbial electrolysis cell-assisted thermophilic anaerobic co-digestion of food waste and spent mushroom substance.

Microbial electrolysis cell-assisted thermophilic anaerobic digestion (MEC-TAD) is a promising method to improve anaerobic co-digestion efficiency; however, its application is restricted by high energy consumption. To improve the energy use efficiency of MEC-TAD, this study investigated the effect of different intermittent energization strategies on thermophilic co-digestion performance. Results revealed that an 18 h-ON/6h-OFF energization schedule resulted in the fastest electron transfer rate and the highest methane yield (364.3 mL/g VS). Mechanistic analysis revealed that 18 h-ON/6h-OFF resulted in the enrichment of electroactive microorganisms and increased abundance of enzyme-coding genes associated with energy metabolism (ntp, nuo, atp), electron transfer (pilA, nfrA2, ssuE), and the hydrogenotrophic methanogenic pathway. Finally, energy balance analysis revealed that 18 h-ON/6h-OFF had the highest net energy benefit (2.52 kJ) and energy conversion efficiency (110.76 %). Therefore, intermittent energization of MEC-TAD using an 18 h-ON/6h-OFF schedule can provide improved performance and more energy savings.

Highly efficient oriented bioconversion of food waste to lactic acid in an open system: Microbial community analysis and biological carbon fixation evaluation.

The valorization of organic solid waste to lactic acid (LA) in open fermentation systems has attracted tremendous interest in recent years. In this study, a highly efficient oriented LA bioconversion system from food waste (FW) in open mode was established. The maximum LA production was 115 g/L, with a high yield of 0.97 g-LA/g-total sugar. FW is a low-cost feedstock for LA production, containing indigenous hydrolysis and LA-producing bacteria (LAB). Saccharification and real-time pH control were found to be essential for maintaining LAB dominantly in open systems. Furthermore, microbial community analysis revealed that Enterococcus mundtii adapted to complex FW substrates and dominated the subsequent bioconversion process. The oriented LA bioconversion exhibited the capacity for biological carbon fixation by reducing CO2 emissions by at least 21 kg per ton of FW under anaerobic conditions.

Effect of biodrying of lignocellulosic biomass on humification and microbial diversity.

By optimizing the carbon to nitrogen (C/N) ratio, this study accomplished an improved level of humification and microbial diversity in the biodrying process of lignocellulosic biomass. The results demonstrated that C/N ratio of 20 accelerated the decomposition of refractory lignocellulose, resulting in lower greenhouse gas emissions and the production of highly mature fertilizer with a germination index of 119.0% and a humic index of 3.2. Moreover, C/N ratio of 20 was found to diversify microbial communities, including Pseudogracilibacillus, Sinibacillus, and Georgenia, which contributed to the decomposition of lignocellulosic biomass and the production of humic acid. Hence, it is recommended to regulate the C/N ratio to 20:1 during the biodrying of biogas residue and wood chips to promote the economic feasibility and bioresource recycling.

Economic and environmental evaluation for a closed loop of crude glycerol bioconversion to biodiesel.

Crude glycerol, a byproduct of biodiesel production, was utilized as a carbon source to produce microbial lipids by the oleaginous yeast Rhodotorula toruloides in this study. The maximum lipid production and lipid content were 10.56 g/L and 49.52%, respectively, by optimizing fermentation conditions. The obtained biodiesel met the standards of China, the United States, and the European Union. The economic value of biodiesel produced from crude glycerol increased by 48% compared with the sale of crude glycerol. In addition, biodiesel production from crude glycerol could reduce 11,928 tons of carbon dioxide emissions and 55 tons of sulfur dioxide emissions. This study provides a strategy for a closed loop of crude glycerol to biofuel and ensures sustainable and stable development of the biodiesel industries.

The stable operation of nitritation process with the continuous granular sludge-type reactor and microbial community analysis.

The nitritation step is the fundament for the biological nitrogen removal regardless of the traditional nitrification and denitrification process, the nitrite shunt process or the anammox process. Thus, exploring the effective nitritation performance is an important aspect of biological nitrogen removal. This study explored the upper limit of nitritation rate by increasing hydraulic residence time with the well-mixed and continuous granular sludge-type reactor characterized with low complexity and easy operation. The results showed that with the nitrogen loading rate of 1.0 kg/m3/d, the nitrite production rate could reach up to 0.65 kg/m3/d with the nitrite production efficiency of 63.49%, which is remarkable compared to that in the previously similar research. The microbial analysis indicated that ammonia-oxidizing bacteria was successfully enriched (13.27%) and genus Nitrosomonas was the dominant bacteria type. Besides, the activity of ammonium oxidizing bacteria in the continuous flow reactor was higher than that of other reactor types. The growth of vorticella on the sludge was also found in the reactor. The test of specific sludge activity and the microbe analysis both indicated that the nitrite-oxidizing bacteria was well inhibited during the whole experiment, which indicated the strategy of simply adjusting the dissolved oxygen is effective for running of nitritation process. The phosphorus removal performance was also achieved with a removal efficiency of 23.53%. The functional composition of the microbial community in the samples was predicted and finally transformation mechanism of nitrogen in sludge was drawn. In sum, this study indicated the superior performance of the granule sludge-type nitritation process and give a reference for the application of biological nitrogen removal technology.

Improving lactic acid yield of hemicellulose from garden garbage through pretreatment of a high solid loading coupled with semi-hydrolysis using low enzyme loading.

Byproduct (acetate and ethanol) generation and carbon catabolite repression are two critical impediments to lactic acid production from the hemicellulose of lignocellulosic biomass. To reduce byproduct generations, acid pretreatment with high solid loading (solid-liquid ratio 1:7) of garden garbage was conducted. The byproduct yield was only 0.30 g/g during in the subsequent lactic acid fermentation from acid pretreatment liquid and 40.8% lower than that of low solid loading (0.48 g/g). Furthermore, semi-hydrolysis with low enzyme loading (10 FPU/g garden garbage cellulase) was conducted to regulate and reduce glucose concentration in the hydrolysate, thereby relieving carbon catabolite repression. During the lactic acid fermentation process, the xylose conversion rate was restored from 48.2% (glucose-oriented hydrolysis) to 85.7%, eventually achieving a 0.49 g/g lactic acid yield of hemicellulose. Additionally, RNA-seq revealed that semi-hydrolysis with low enzyme loading down-regulated the expression of ptsH and ccpA, thereby relieving carbon catabolite repression.

A review on nirS-type and nirK-type denitrifiers via a scientometric approach coupled with case studies.

The denitrification process plays an important role in improving water quality and is a source/sink of nitrous oxide to the atmosphere. The second important rate-limiting step of the denitrification process is catalyzed by two enzymes with different structures and unrelated evolutionary relationships, namely, the Cu-type nitrite reductase encoded by the nirK gene and the cytochrome cd1-type nitrite reductase encoded by the nirS gene. Although some relevant reviews have been published on denitrifiers, most of these reviews do not include statistical analysis, and do not compare the nirS and nirK communities in-depth. However, a systematic study of the nirS-type and nirK-type denitrifying communities and their response to environmental factors in different ecosystems is needed. In this review, a scientometric approach combined with case studies was used to study the nirS-type and nirK-type denitrifiers. The scientometric approach demonstrated that Pseudomonas, Paracoccus, and Thauera are the most frequently mentioned nirS-type denitrifiers, while Pseudomonas and Bradyrhizobium are the top two most frequently mentioned nirK-type denitrifiers. Among various environmental factors, the concentrations of nitrite, nitrate and carbon sources were widely reported factors that can influence the abundance and structure of nirS-type and nirK-type denitrifying communities. Case studies indicated that Bradyrhizobium was the major genus detected by high-throughput sequencing in both nirS and nirK-type denitrifiers in soil systems. nirS-type denitrifiers are more sensitive to the soil type, soil moisture, pH, and rhizosphere effect than nirK. To clarify the relationships between denitrifying communities and environmental factors, the DNA stable isotope probe combined with metagenomic sequencing is needed for new denitrifier detections.