RESUMO
Rat hepatitis E virus (HEV) is related to human HEV and has been detected in wild rats worldwide. Here, the complete genome of rat HEV strain R63/DEU/2009 was cloned downstream of the T7 RNA polymerase promoter and capped genomic RNA generated by in vitro transcription was injected into nude rats. Rat HEV RNA could be detected in serum and faeces of rats injected intrahepatically, but not in those injected intravenously. Rat HEV RNA-positive faecal suspension was intravenously inoculated into nude rats and Wistar rats leading to rat HEV RNA detection in serum and faeces of nude rats, and to seroconversion in Wistar rats. In addition, rat HEV was isolated in PLC/PRF/5 cells from the rat HEV RNA-positive faecal suspension of nude rats and then passaged. The cell culture supernatant was infectious for nude rats. Genome analysis identified nine point mutations of the cell-culture-passaged virus in comparison with the originally cloned rat HEV genome. The results indicated that infectious rat HEV could be generated from the cDNA clone. As rats are widely used and well-characterized laboratory animals, studies on genetically engineered rat HEV may provide novel insights into organ tropism, replication and excretion kinetics as well as immunological changes induced by hepeviruses.
Assuntos
DNA Complementar/genética , DNA Viral/genética , Vírus da Hepatite E/genética , Vírus da Hepatite E/fisiologia , RNA Viral/genética , Animais , Clonagem Molecular/métodos , Fezes/virologia , Feminino , Injeções Intravenosas , Masculino , RNA Viral/biossíntese , Ratos Nus , Ratos Wistar , Soro/virologia , Transcrição Gênica , Virologia/métodosRESUMO
Ferret hepatitis E virus (HEV), a novel hepatitis E-like virus, has been identified in ferrets in the Netherlands, Japan, and the US. To determine whether ferret HEV transmits to other animals, we inoculated laboratory rats (Wistar), nude rats (Long-Evans-rnu/rnu), and cynomolgus monkeys with ferret HEV (F4351) by intravenous injection. None of the animals demonstrated a positive sign for virus replication, indicating that rats and monkeys are not susceptible to ferret HEV.
Assuntos
Suscetibilidade a Doenças/veterinária , Furões , Vírus da Hepatite E/imunologia , Vírus da Hepatite E/fisiologia , Hepatite E/veterinária , Doenças dos Macacos/virologia , Doenças dos Roedores/virologia , Animais , Anticorpos Anti-Hepatite/sangue , Hepatite E/transmissão , Hepatite E/virologia , Macaca fascicularis , RNA Viral , Ratos , Ratos Long-Evans , Ratos Wistar , Replicação ViralRESUMO
The complete genome of hepatitis E virus (HEV) from laboratory ferrets imported from the United States was identified. This virus shared only 82.4%-82.5% nt sequence identities with strains from the Netherlands, which indicated that the ferret HEV genome is genetically diverse. Some laboratory ferrets were contaminated with HEV.
Assuntos
Furões/virologia , Genoma Viral , Vírus da Hepatite E/genética , Animais , Variação Genética , Hepatite E/virologia , Países Baixos , Filogenia , Análise de Sequência de DNA/métodos , Estados UnidosRESUMO
We amplified the complete genome of the rat hepatitis E virus (HEV) Vietnam strain (V-105) and analyzed the nucleotide and amino acid sequences. The entire genome of V-105 shared only 76.8%-76.9% nucleotide sequence identities with rat HEV strains from Germany, which suggests that V-105 is a new genotype of rat HEV.
Assuntos
Animais Selvagens/virologia , Genoma Viral , Vírus da Hepatite E/genética , Hepatite E/virologia , RNA Viral/genética , Ratos/virologia , Animais , Sequência de Bases , Primers do DNA , Genótipo , Vírus da Hepatite E/classificação , Vírus da Hepatite E/isolamento & purificação , Dados de Sequência Molecular , Tipagem Molecular , Filogenia , RNA Viral/classificação , RNA Viral/isolamento & purificação , Ratos Wistar , Homologia de Sequência do Ácido Nucleico , VietnãRESUMO
We examined 85 fecal samples from pet ferrets in 10 animal hospitals in Japan for the detection of ferret hepatitis E virus (HEV) RNA. We found that 6 (7.1%) of the samples were positive for ferret HEV RNA. Phylogenetic analysis based on the partial ORF1 indicated that these ferret HEV strains were clearly separated from the Netherlands strains and were divided into 2 distinct clusters. These results suggest that ferret HEV is genetically diverse, and since ferrets are not indigenous to Japan, ferret HEV has been introduced into Japan through importation.