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1.
Arch Bronconeumol ; 45(5): 230-4, 2009 May.
Artigo em Espanhol | MEDLINE | ID: mdl-19371995

RESUMO

INTRODUCTION: Chronic airflow obstruction in conditions such as chronic obstructive pulmonary disease is associated with respiratory muscle dysfunction. Our aim was to study the effects of salbutamol-a beta-adrenergic agonist known to improve muscle strength in physiologic and pathologic conditions-on diaphragm contractility in an animal model of chronic airway obstruction achieved by tracheal banding. MATERIALS AND METHODS: Twenty-four Sprague-Dawley rats were randomized into a control group and 3 tracheal banding groups, 1 that received acute salbutamol treatment, 1 that received chronic salbutamol treatment, and 1 that received nothing. Arterial blood gases, acid-base balance, and in vitro diaphragmatic contractility were evaluated by measuring peak twitch tension, contraction time, contraction velocity, half-relaxation time, relaxation velocity, and force-frequency curves. RESULTS: The 3 study groups had significantly reduced arterial pH and increased PaCO2 and bicarbonate levels compared to the control group (P<.05). The untreated tracheal banding group had significantly reduced peak twitch tension and contraction velocity, and a significantly lower force-frequency curve in comparison with the other groups (P<.05). The chronic treatment group had a higher relaxation velocity than the untreated study group (P<.05). The mean (SE) peak twitch tension values were 6.46 (0.90)N/cm(2) for the control group, 3.28 (0.55)N/cm(2) for the untreated tracheal banding group, 6.18 (0.71)N/cm(2) for the acute treatment group, and 7.09 (0.59)N/cm(2) for the chronic treatment group. CONCLUSIONS: Diaphragmatic dysfunction associated with chronic airflow obstruction improves with both the acute and chronic administration of salbutamol. The mechanisms involved in respiratory muscle dysfunction warrant further study.


Assuntos
Agonistas Adrenérgicos beta/uso terapêutico , Obstrução das Vias Respiratórias/tratamento farmacológico , Albuterol/uso terapêutico , Diafragma/efeitos dos fármacos , Agonistas Adrenérgicos beta/farmacologia , Obstrução das Vias Respiratórias/sangue , Obstrução das Vias Respiratórias/fisiopatologia , Albuterol/farmacologia , Alcalose/sangue , Alcalose/etiologia , Alcalose/prevenção & controle , Animais , Doença Crônica , Diafragma/fisiopatologia , Avaliação Pré-Clínica de Medicamentos , Hipercapnia/sangue , Hipercapnia/etiologia , Hipercapnia/prevenção & controle , Masculino , Contração Muscular/efeitos dos fármacos , Relaxamento Muscular/efeitos dos fármacos , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley
2.
Curr Protoc Immunol ; 120: 24.1.1-24.1.25, 2018 02 21.
Artigo em Inglês | MEDLINE | ID: mdl-29512144

RESUMO

Traumatic brain injury (TBI) is a leading cause of death and disability and is a risk factor for the later development of neuropsychiatric disorders and neurodegenerative diseases. Many models of TBI have been developed, but their further refinement and a more detailed long-term follow-up is needed. We have used the Thy1-YFP-H transgenic mouse line and the parallel rod floor test to produce an unbiased and robust method for the evaluation of the multiple effects of a validated model of controlled cortical injury. This approach reveals short- and long-term progressive changes, including compromised biphasic motor function up to 85 days post-lesion, which correlates with neuronal atrophy, dendrite and spine loss, and long-term axonal pathology evidenced by axon spheroids and fragmentation. Here we present methods for inducing a controlled cortical injury in the Thy1-YFP-H transgenic mouse line and for evaluating the resulting deficits in the parallel rod floor test. This technique constitutes a new, unbiased, and robust method for the evaluation of motor and behavioral alterations after TBI. © 2018 by John Wiley & Sons, Inc.


Assuntos
Lesões Encefálicas Traumáticas , Modelos Animais de Doenças , Animais , Astrócitos/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Comportamento Animal , Encéfalo/metabolismo , Encéfalo/patologia , Lesões Encefálicas Traumáticas/diagnóstico por imagem , Lesões Encefálicas Traumáticas/patologia , Lesões Encefálicas Traumáticas/fisiopatologia , Feminino , Proteína Glial Fibrilar Ácida/metabolismo , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Exame Neurológico , Neurônios/metabolismo , Neurônios/patologia , Ratos Sprague-Dawley
3.
In. Manzanares Castro, William; Aramendi Epstein, Ignacio; Pico, José Luis do. Disionías en el paciente grave: historias clínicas comentadas. Montevideo, Cuadrado, 2021. p.51-68, tab.
Monografia em Espanhol | LILACS, UY-BNMED, BNUY | ID: biblio-1344692
4.
In. Verga, Federico; Burghi, Gastón. Encares de paciente crítico. Montevideo, Oficina del Libro FEFMUR, 2020. p.41-57, tab, ilus.
Monografia em Espanhol | LILACS, UY-BNMED, BNUY | ID: biblio-1342636
5.
Montevideo; CLAEH; 2019. 270 p. ilus, tab, graf.(Medicina Esenciales, 1).
Monografia em Espanhol | LILACS, UY-BNMED, BNUY | ID: biblio-1342457
6.
In. Boggia, José; López, Alejandra; Bianchi, Sergio; Noboa, Oscar; Gadola, Liliana; Briva, Arturo; Hurtado, Javier; Grignola, Juan Carlos; Rodríguez, MaríaJosé. Fisiopatología: mecanismos de las disfunciones orgánicas. Montevideo, Oficina del Libro FEFMUR, 2a. ed; 2011. p.105-161.
Monografia em Espanhol | LILACS | ID: lil-759798
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