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1.
Res Vet Sci ; 155: 150-155, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36696786

RESUMO

The present study compared multiplex PCR (mPCR) and Whole Genome Sequencing (WGS) using the SCCmecFinder database to identify the Staphylococcal Cassette Chromosome (SCC) mec in five Staphylococcus aureus (SA) and nine non-aureus staphylococci (NAS) isolated from dairy cattle. mPCR identified an SCCmecIV in four SA and one NAS, but could not differentiate between SCCmecII and IV in the fifth SA, that all harbored the mecA gene and were phenotypically resistant to cefoxitin. SCCmecFinder confirmed the presence of an SCCmecIVc(2B) in four SA and of the SCCmecIVa(2B) in the fifth SA and the one NAS. Both methods also detected one untypeable SCCmec in another cefoxitin-resistant NAS harboring the mecA gene and a pseudo SCCmec in one cefoxitin-sensitive NAS harboring one mecC-related gene. No SCCmec elements were identified either in one cefoxitin-sensitive NAS harboring the mecA2 gene, or in five NAS (one resistant and four sensitive to cefoxitin) harboring the mecA1 gene. SCCmecFinder could even not identify the presence of any mecA1 gene in these five NAS, whose presence was nevertheless confirmed by ResFinder. The conclusions of this study are: (i) mPCR and WGS sequencing using SCCmecFinder are complementary methodologies to identify SCCmec; (ii) SCCmecFinder and ResFinder to a lesser extent cannot identify all mec gene allotypes; (iii) a specific classification of the SCCmec in NAS would be epidemiologically helpful; (iv) presence of a mecA gene and a complete SCCmec is linked to cefoxitin resistance, whereas presence of other mec genes and of pseudo or no SCCmec is not.


Assuntos
Doenças dos Bovinos , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Animais , Bovinos , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Bélgica , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/microbiologia , Cefoxitina/farmacologia , Cromossomos , Testes de Sensibilidade Microbiana/veterinária , Reação em Cadeia da Polimerase Multiplex/veterinária , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/veterinária , Staphylococcus/genética , Staphylococcus aureus/genética , Sequenciamento Completo do Genoma/veterinária
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