A global effect of capture saccades.

When two target elements are presented in close proximity, the endpoint of a saccade is generally positioned at an intermediate location ('global effect'). Here, we investigated whether the global effect also occurs for eye movements executed to distracting elements. To this end, we adapted the oculomotor capture paradigm such that on a subset of trials, two distractors were presented. When the two distractors were closely aligned, erroneous eye movements were initiated to a location in between the two distractors. Even though to a lesser extent, this effect was also present when the two distractors were presented further apart. In a second experiment, we investigated the global effect for eye movements in the presence of two targets. A strong global effect was observed when two targets were presented closely aligned, while this effect was absent when the targets were further apart. This study shows that there is a global effect when saccades are captured by distractors. This 'capture global' effect is different from the traditional global effect that occurs when two targets are presented because the global effect of capture saccades also occurs for remote elements. The spatial dynamics of this global effect will be explained in terms of the population coding theory.

Oculomotor capture in ADHD.

It is generally thought that deficits in response inhibition form an important area of dysfunction in patients with attention-deficit/hyperactivity disorder (ADHD). However, recent research using visual search paradigms seems to suggest that these inhibitory deficits do not extend towards inhibiting irrelevant distractors. Using an oculomotor capture task, the present study investigated whether boys with ADHD and their nonaffected brothers are impaired in suppressing reflexive eye movements to a task-irrelevant onset distractor. Results showed that boys with ADHD had slower responses than controls, but were as accurate in their eye movements as controls. Nonaffected brothers showed similar problems in the speed of responding as their affected brothers, which might suggest that this deficit relates to a familial risk for developing the disorder. Importantly, all three groups were equally captured by the distractor, which shows that boys with ADHD and their brothers are not more distracted by the distractor than are controls. Saccade latency and the proportion of intrusive saccades were related to continuous dimensions of ADHD symptoms, which suggests that these deficits are not simply present or absent, but rather indicate that the severity of these deficits relate to the severity of ADHD. The finding that boys with ADHD (and their nonaffected brothers) did not have problems inhibiting irrelevant distractors contradicts a general response inhibition deficiency in ADHD, which may be explained by the relatively independency of working memory in this type of response inhibition.

Immunocytochemical characterization of the expression of inducible and constitutive isoforms of nitric oxide synthase in demyelinating multiple sclerosis lesions.

The cellular localization and distribution of inducible and constitutive nitric oxide synthase (iNOS/cNOS) was determined in tissue sections from multiple sclerosis (MS) and control brain and spinal cord. Immunocytochemical techniques were applied using specific iNOS- and cNOS-directed antibodies. In addition, NADPH-diaphorase histochemistry was performed. To establish the identity of iNOS-, cNOS- and NADPH-diaphorase-positive cells single and double staining was performed on tissue sections with the macrophage marker KP1 (CD68) and with the astrocyte marker glial fibrillary acidic protein (GFAP). Areas of myelin breakdown and demyelination were determined using a staining for neutral lipids, Oil Red O (ORO). Furthermore, macrophages isolated from active demyelinating MS lesions were stained for iNOS, cNOS, KP1 and ORO. In active MS lesions strong iNOS immunoreactivity was found exclusively in perivascular and parenchymal macrophages distributed within regions of active demyelination. In these active MS lesions immunoreactivity for cNOS was also found in macrophages. Macrophages isolated from active MS lesions also showed immunoreactivity for iNOS and cNOS. Moreover, these isolated macrophages produced nitric oxide (NO; >30 microM) in vitro. NADPH-diaphorase activity was detected in KP1-positive perivascular and parenchymal macrophages and in GFAP-positive reactive astrocytes in active MS lesions and in reactive astrocytes located in the hypercellular rims of chronic active MS lesions. cNOS-positive reactive astrocytes were detected in both active and chronic active MS lesions. Inside chronic active lesions some residual macrophages were weakly iNOS-positive. In control brain and spinal cord no iNOS immunoreactivity could be detected. These results suggests an important role for human macrophages capable of producing the free radical nitric oxide (NO), which may contribute to the cytotoxicity of oligodendrocytes and destruction of myelin in MS brain and spinal cord.

Postmortem delay effects on neuroglial cells and brain macrophages from Lewis rats with acute experimental allergic encephalomyelitis: an immunohistochemical and cytochemical study.

The effects of increasing postmortem delay (PMD) times on morphological, immunological and functional characteristics of various brain cells both in situ and in vitro were studied in postmortem brain tissue derived from rats with acute experimental allergic encephalomyelitis (EAE). A decline of the brain tissue structure was first noted after a PMD of 6 h. Radial glia in the cerebellum were frequently interrupted and retractions artifacts appeared around brain cells. However, even after the longest PMD interval of 18 h the quality of the cell and tissue structure was still good enough for immunohistochemical characterization. Immunohistochemical staining of frozen and fixed rat brain tissue sections resulted in an enhancement of the immunoreactivity after a PMD of 4 h, using a panel of mono and polyclonal antibodies directed against glial fibrillary acidic protein (GFAP), basement membranes (laminin), brain macrophage antigens (ED1 and ED2), and various immunologically important surface molecules, such as major histocompatibility complex (MHC) class II (Ia) antigen (OX6), CR3 complement receptor (ED8), and leukocyte common antigen (OX1). No increase in staining intensities with the ED1, ED8 and OX6 mAbs specific for macrophage antigens could be detected on brain macrophages that were isolated from brain tissue of rats with EAE obtained after various PMD intervals. Irrespective of the PMD interval, viable astrocyte cell cultures were obtained with comparable staining intensities for GFAP. These cultured astrocytes were capable of ingesting Latex beads and were highly proliferative as measured by BrdU uptake, at all investigated PMDs. Thus, even after long PMD intervals, brain material can be used successfully. Other data suggest that the situation is similar to human brain material, even though the PMD times may be somewhat different.

The antiproliferative effect of 8-chloro-adenosine, an active metabolite of 8-chloro-cyclic adenosine monophosphate, and disturbances in nucleic acid synthesis and cell cycle kinetics.

8-Chloro-adenosine, the dephosphorylated metabolite of the antineoplastic agent 8-chloro-cyclic AMP, has been proposed to act on the regulatory subunits of cyclic AMP-dependent protein kinase. 8-Chloro-adenosine has a growth-inhibitory effect, the mechanism of which is unclear. We investigated the effects of 8-chloro-cyclic AMP and 8-chloro-adenosine on nucleic acid synthesis and cell cycle kinetics in two human glioma cell lines. These effects were compared to those of the cyclic AMP analogue 8-(4-chlorophenyl)-thio-cyclic AMP (8-CPTcAMP), which is less susceptible to dephosphorylation. Whereas 8-CPTcAMP almost completely inhibited RNA and DNA synthesis, both 8-chloro-adenosine and 8-chloro-cyclic AMP only partly inhibited synthesis of RNA and DNA at growth-inhibitory concentrations, as demonstrated by using [5-1H] uridine and [14C]thymidine incorporation. Therefore, the growth-inhibitory effect of 8-chloro-cyclic AMP is not (or not completely) due to activation of cyclic AMP-dependent protein kinase nor to the inhibition of nucleic acid synthesis. Flow cytometric analysis revealed that 8-chloro-cyclic AMP and 8-chloro-adenosine probably block cell cycle progression at the G2M phase. The effects of 8-chloro-cyclic AMP on nucleic acid synthesis and cell cycle progression were largely prevented by adenosine deaminase, which inactivates 8-chloro-adenosine. This indicates that the effects of 8-chloro-cyclic AMP were at least in part due to its metabolite 8-chloro-adenosine. Incorporation of 8-chloro-adenosine into RNA and DNA might contribute to the disturbance of the cell cycle kinetics and growth-inhibitory effect of 8-chloro-adenosine.

Stimulus-driven capture and attentional set: selective search for color and visual abrupt onsets.

Recent evidence suggests that attentional capture is contingent on the attentional control setting induced by the task demands (C. L. Folk, R. Remington, & J. C. Johnston, 1992). Because the experiments on which these conclusions are based can be criticized for several reasons, the contingent capture hypothesis was tested using 2 visual search tasks in which subjects searched multielement displays in which a color singleton and onset singleton were simultaneously present. Both experiments show that the contingent capture hypothesis does not hold: Irrespective of attentional set, attention was captured by the most salient singleton. The findings suggest a stimulus-driven model of performance in which selection is basically determined by the properties of the featural singletons present in the visual field.

Attentional control during visual search: the effect of irrelevant singletons.

Four experiments investigated whether a highly salient color singleton can be ignored during serial search. Observers searched for a target letter among nontarget letters and were instructed to ignore an irrelevant, highly salient color singleton that was either compatible or incompatible with the response to the target letter. The results indicate that it was possible to prevent attentional capture by the irrelevant singleton only when both the target and the distractor color were known. When either the color of the target or the color of the to-be-ignored singleton were varied over trials, the irrelevant singleton captured attention. The ability to selectively filter singleton distractors during serial search depends on the presence of an attentional set for a specific feature value of both target and distractor. In the absence of a consistently predictable feature value of both target and distractor, top-down control is not possible.

Attentional control within 3-D space.

Four experiments investigated whether directing attention to a particular plane in depth enables observers to filter out information from another depth plane. Observers viewed stereoscopic displays and searched for a red line segment among green line segments. The results showed that directing attention to a particular depth plane cannot prevent attentional capture from another depth plane when the colors of the target and distractor are identical. However, it can prevent attentional capture by a singleton from another depth plane when the colors of the target and distractor are different. These results indicate that only when both color and depth information are selective in guiding attention to the target singleton can attentional capture by irrelevant singletons be prevented. The results also suggest that retinal disparity does not have the same special status as location information in two dimensions and should be considered as just another feature along which selection may occur.

Attentional effects on preattentive vision: spatial precues affect the detection of simple features.

Most accounts of visual perception hold that the detection of primitive features occurs preattentively, in parallel across the visual field. Evidence that preattentive vision operates without attentional limitations comes from visual search tasks in which the detection of the presence of absence of a primitive feature is independent of the number of stimuli in a display. If the detection of primitive features occurs preattentively, in parallel and without capacity limitations, then it should not matter where attention is located in the visual field. The present study shows that even though the detection of a red element in an array of gray elements occurred in parallel without capacity limitations, the allocation of attention did have a large effect on search performance. If attention was directed to a particular region of the display and the target feature was presented elsewhere, response latencies increased. Results indicate that the classic view of preattentive vision requires revision.

Influence of attentional capture on oculomotor control.

Previous research has shown that when searching for a color singleton, top-down control cannot prevent attentional capture by an abrupt visual onset. The present research addressed whether a task-irrelevant abrupt onset would affect eye movement behavior when searching for a color singleton. Results show that in many instances the eye moved in the direction of the task-irrelevant abrupt onset. There was evidence that top-down control could neither entirely prevent attentional capture by visual onsets nor prevent the eye from starting to move in the direction of the onset. Results suggest parallel programming of 2 saccades: 1 voluntary goal-directed eye movement toward the color singleton target and 1 stimulus-driven eye movement reflexively elicited by the abrupt onset. A neurophysiologically plausible model that can account for the current findings is discussed.

An adaptation-induced pop-out in visual search.

The present study demonstrates that an object embedded in an array of identical objects can pop-out. Dependent on the stimuli preceding the search display, local (chromatic) adaptation causes an identical object to pop-out because it appears to have a colour (Expt 1) or brightness (Expt 2) that is slightly different from the colour and brightness of the other objects in the display. Experiment 3 shows that this pop-out even occurs when the stimulus preceding the search display is presented for only 100 msec.

Parallel search for a conjunction of contrast polarity and shape.

When a target object embedded in an array of other objects can be distinguished along a single feature dimension (e.g. color or shape), it can be detected in parallel. When a target object is defined by a conjunction of stimulus features, at least some serial search is required, indicating that the visual system is less efficient in conducting a parallel search over two stimulus dimensions simultaneously. Some exceptions to this finding have been reported. The present results suggest another exception: search for a conjunction of contrast polarity and shape can be conducted in parallel while the same conjunction consisting of color and shape requires some serial search. The neurophysiological implications are discussed.

Attentional capture and aging: implications for visual search performance and oculomotor control.

Two studies examined potential age-related differences in attentional capture. Subjects were instructed to move their eyes as quickly as possible to a color singleton target and to identify a small letter located inside it. On half the trials, a new stimulus (i.e., a sudden onset) appeared simultaneously with the presentation of the color singleton target. The onset was always a task-irrelevant distractor. Response times were lengthened, for both young and old adults, whenever an onset distractor appeared, despite the fact that subjects reported being unaware of the appearance of the abrupt onset. Eye scan strategies were also disrupted by the appearance of the onset distractors. On about 40% of the trials on which an onset appeared, subjects made an eye movement to the task-irrelevant onset before moving their eyes to the target. Fixations close to the onset were brief, suggesting parallel programming of a reflexive eye movement to the onset and goal-directed eye movement to the target. Results are discussed in terms of age-related sparing of the attentional and oculomotor processes that underlie attentional capture.

Effects of location and form cuing on the allocation of attention in the visual field.

Two experiments were conducted which tested the effect of two different types of cues on the allocation of attention in the visual field. Subjects responded to a target appearing either 6.5 degrees to the right or to the left of fixation in a field filled with small randomly positioned line segments. The target form was either a circle or diamond in which a horizontal or vertical line segment was positioned. Subjects made a speeded discrimination response on the basis of the orientation of this line segment. In the first experiment, there was always one target at each trial. In the second experiment, both circle and diamond were presented left or right of fixation simultaneously whereby the line segment only appeared in one of the forms. In different conditions, the most likely target location (left or right) and form (circle or diamond) were cued. Control conditions served as a baseline for determining costs and benefits. Reaction time measures showed that the validity of the location cue resulted in both costs and benefits, whereas the validity of the form cue had no such effects. The results cannot be reconciled with the claim of zoom lens theories that spatial attention can switch between different modes of operations. They are also at odds with theories that claim that attention has no spatial locus. The results are consistent with a simple spotlight theory in which spatial attention involves selecting a particular restricted area of the visual field for which the perceptual efficiency is enhanced.

Perceptual selectivity is task dependent: evidence from selective search.

Four visual search experiments examined the allocation of attention in a multi-item display with a salient stimulus difference. If, irrespective of its relevance to performance, an item with a unique property attracts attention, it can be said that the unique property automatically captures attention. Alternatively, if capturing attention depends on the relevance of the unique property it would suggest that subjects are capable of engaging in active filtering. In the experiments, subjects searched visually for a horizontal line segment positioned in either one of the 4, 8 or 16 surrounds, arranged in an imaginary circle around the fixation point. In each trial, there was always one surround that differed from the others. The data showed that attention was not automatically attracted to the exceptional form (experiment 1) or exceptional color (experiment 2). The control conditions showed that form and color differences could serve as the basis for active allocation of attention to the unique item. Attention capturing is occasionally observed when the surround changes abruptly in form (experiment 3) but not when it changes abruptly in color (experiment 4). Implications of these findings are discussed in relation to theories of attention that distinguish between a first, parallel stage of perceptual processing followed by a second stage of limited capacity. The results are compatible with the notion that pre-attentive processing is under strategic control and can be stopped by filtering on a spatial basis. The results are related to the specific properties of the transient channels in the visual system.

Categorization and identification of simultaneous targets.

Early and late selection theories of visual attention disagree about whether identification occurs before or after selection. Studies showing the category effect, i.e., the time to detect a letter is hardly affected by the number of digits present in the display, are taken as evidence for late selection theories since these studies suggest parallel identification of all items in the display. As an extension of previous studies, in the present study two categorically different targets were presented simultaneously among a variable number of nontargets. Subjects were shown brief displays of two target letters among either 2, 4 or 6 nontarget digits. Subjects responded 'same' when the two letters were identical and 'different' otherwise. Since the 'same-different' response reflects the combined outcome of the simultaneous targets, late-selection theory predicts that the time to match the target letters is independent of the number of nontarget digits. Alternatively, early-selection theory predicts a linear increase of reaction time with display size since the presence of more than one target disrupts parallel preattentive processing, leading to a serial search through all items in the display. The results provide evidence for the early-selection view since reaction time increased linearly with the number of categorically different nontargets. A control experiment revealed that none of the alternative explanations could account for the display size effect.

Visual selective attention: a theoretical analysis.

The present paper outlines a framework which allows a consistent interpretation of data regarding visual selection in visual search tasks. It organizes and reviews visual search tasks in which the target is defined by primitive features, by conjunctions of features and when the target is categorically different from non-targets. The special role of spatial attention is reviewed and different theoretical accounts are discussed. Because visual selection depends principally on the outcome of the early parallel preattentive stage of processing, the main focus will be on this stage. It is concluded that visual selection is to a large extent determined by the physical characteristics of the stimuli present in the visual field. The early preattentive parallel process computes how different each object is from each of the other objects within a particular stimulus dimension. Attention is automatically drawn to the location having the highest activation, implying that the object at that location is automatically selected irrespective of the intentions of the subject. The model also assumes some top-down control. It is well known that attention can be voluntarily directed to nonfixated locations in visual space, varying from a uniform distribution over the visual field to a highly focused concentration. The model assumes that the endogenous direction of attention to an area in the visual field is the only top-down manner of affecting visual selection. Within the area of directed attention, no top-down control is possible: selection is completely determined by the physical properties of the stimuli.

Spatial attention in early vision.

The present study addressed whether the allocation of attention to a particular region in space can prevent processing of distractor information from non-attended regions. A cue indicated the area in visual space where the target singleton would be presented. Observers were required to detect this target singleton and ignore a distractor singleton presented within a non-attended region. The results indicate that the allocation of attention to a region in space cannot prevent the processing of unwanted information from elsewhere in the visual field. It is concluded that the function of the allocation of attention is not to enhance the processing capacity within the attended region but rather to attenuate interference from distractors in unattended regions.

Daytime running lights as a vehicle collision countermeasure: the Swedish evidence reconsidered.

In Sweden the use of daytime running lights (DRL) was made mandatory on 1 October 1977 for all motor vehicles at once, during all seasons and for all areas. According to a study conducted by Andersson and Nilsson (1981) [Andersson and Nilsson. VTI Swedish Road and Transport Research Institute, Report No. 208A; 1981] the introduction of DRL resulted in a reduction of 11% of multiple accidents during daytime. In many discussions on the effectiveness of DRL, these findings have been considered as the strongest evidence that the use of DRL is an effective vehicle collision countermeasure. The present study reexamines this evidence and shows that the reported 11% effect of DRL in the Swedish study is spurious. The effect is mainly the result of the application of a model that shows selective effects of DRL through modelling of unexplained changes in the number of single accidents. It is concluded that the Swedish data fail to show a clear effect of DRL.

The effectiveness of side marker lamps: an experimental study.

The present experiment investigated the effect of small amber lamps mounted near the front and rear on each side of a passenger car-so called side marker lamps-on visual detection and recognition of passenger cars in complex nighttime environments. It was determined whether cars equipped with side marker lamps are detected and recognized earlier and more accurately than cars without side marker lamps. Subjects were presented with slides of natural nighttime scenes in which a car, either with or without side marker lamps, viewed from its side, approaching from a side street, was either present or not. Subjects determined as fast as possible whether a car was present or not. Reaction time measures (speed and accuracy) indicated that both under clear and fog visibility conditions, a car equipped with side marker lamps was detected and recognized earlier and more accurately than a car without side marker lamps. The results indicate that side marker lamps increase both lateral conspicuity and recognizability suggesting that side marker lamps may be effective in reducing the number of nighttime angle collisions.