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1.
Rev Med Chil ; 143(3): 337-44, 2015 Mar.
Artigo em Espanhol | MEDLINE | ID: mdl-26005820

RESUMO

BACKGROUND: In a rapidly changing culture like ours, with emphasis on productivity, there is a strong need to find the meaning of health care work using learning instances that privilege reflection and face to face contact with others. The Diploma in Health and Humanization (DSH), was developed as an interdisciplinary space for training on issues related to humanization. AIM: To analyze the experience of DSH aiming to identify the elements that students considered key factors for the success of the program. MATERIAL AND METHODS: We conducted a focus group with DSH graduates, identifying factors associated with satisfaction. Transcripts were coded and analyzed by two independent reviewers. RESULTS: DSH graduates valued a safe space, personal interaction, dialogue and respect as learning tools of the DSH. They also appreciates the opportunity to have emotional interactions among students and between them and the teacher as well as the opportunity to share personal stories and their own search for meaning. DISCUSSION: DSH is a learning experience in which their graduates value the ability to think about their vocation and the affective interaction with peers and teachers. We hope to contribute to the development of face to face courses in the area of humanization. Face to face methodology is an excellent teaching technique for contents related to the meaning of work, and more specifically, to a group of learners that require affective communication and a personal connection of their work with their own values and beliefs.


Assuntos
Pessoal de Saúde/educação , Humanismo , Relações Interpessoais , Aprendizagem , Adulto , Idoso , Feminino , Grupos Focais , Humanos , Masculino , Pessoa de Meia-Idade , Motivação , Satisfação Pessoal , Pesquisa Qualitativa , Adulto Jovem
2.
Plants (Basel) ; 13(6)2024 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-38592747

RESUMO

The species Senecio nutans Sch. Bip., commonly called "chachacoma", is widely used as a medicinal plant by the Andean communities of Northern Chile. Ethanolic extracts of S. nutans and the main compound, 4-hydroxy-3-(3-methyl-2-butenyl) acetophenone, have shown interesting biological activity. However, due to the high-altitude areas where this species is found, access to S. nutans is very limited. Due to the latter, in this work, we carried out micropropagation in vitro and ex vitro adaptation techniques as an alternative for the massive multiplication, conservation, and in vitro production of high-value metabolites from this plant. The micropropagation and ex vitro adaptation techniques were successfully employed, and UHPLC-DAD analysis revealed no significant changes in the phenolic profile, with acetophenone 4 being the most abundant metabolite, whose antioxidant and antibacterial activity was studied. Independently of the applied culture condition, the ethanolic extracts of S. nutans presented high activity against both Gram-positive and Gram-negative bacteria, demonstrating their antimicrobial capacity. This successful initiation of in vitro and ex vitro cultures provides a biotechnological approach for the conservation of S. nutans and ensures a reliable and consistent source of acetophenone 4 as a potential raw material for pharmacological applications.

3.
Med Teach ; 35(1): e861-6, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-22938681

RESUMO

BACKGROUND: Students' perceptions of their educational environment (EE) have been studied in undergraduate and postgraduate curricula. Postgraduate EE has been measured in hospital settings. However, there are no instruments available to measure the EE in postgraduate ambulatory settings. AIM: The aim of this study was to develop the "ambulatory care learning education environment measure" (ACLEEM). METHODS: A mixed methodology was used including three stages: (1) Grounded theory (focus groups); (2) Delphi technique to identify consensus; and (3) Pilot study. RESULTS: Three quota samples of approximately 60 stakeholders were formed, one as focus groups and two as Delphi panels. Eight focus groups were carried out including 58 residents (Latin-American Spanish speakers). The results were analysed and 173 items were offered to a National Delphi panel (61 residents and teachers). They reduced in two rounds the number of important items to 54. The 54-item questionnaire was then piloted with 63 residents and refined to the final version of the ACLEEM with 50 items and three domains. CONCLUSIONS: The 50-item inventory is a valid instrument to measure the EE in postgraduate ambulatory setting in Chile. Large-scale administration of the ACLEEM questionnaire to evaluate its construct validity and reliability are the next steps to test the psychometric properties of the instrument.


Assuntos
Instituições de Assistência Ambulatorial , Ambiente de Instituições de Saúde , Corpo Clínico Hospitalar/educação , Inquéritos e Questionários/normas , Técnica Delphi , Grupos Focais , Humanos , Corpo Clínico Hospitalar/psicologia , Projetos Piloto , Projetos de Pesquisa
4.
Microorganisms ; 11(11)2023 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-38004648

RESUMO

The Region of Arica and Parinacota (Atacama Desert) offers several unexplored remote sites with unique characteristics that would allow for the formulation of new bioproducts for agriculture. Among them, Jurasi Hot Springs, Polloquere Hot Springs, and Amuyo Lagoons represent a group of open pools fed by thermal water springing from the mountains. Their microbiomes remain unspecified, providing a unique opportunity to characterize the endemic community of these sites and develop new bioproducts for sustainable agriculture. Bacteria were isolated from the sediments of these geothermal sites and characterized by sequencing the 16S rRNA gene, microbiological characterization, and agricultural functional characterization. A total of 57 bacteria were isolated from three geothermal sites north of the Atacama Desert. The sequence analysis showed that the isolates belong to several bacterial genera, including Pantoea, Bacillus, and Pseudomonas, among others. The functional characterization revealed the presence of PGP traits, hydrolytic enzymes, and biocontrol activity against phytopathogenic fungi. These bacteria possess the potential to develop new biobased products for agriculture in arid conditions.

5.
Pharmaceuticals (Basel) ; 16(10)2023 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-37895810

RESUMO

This work proposes the design of ß-keto esters as antibacterial compounds. The design was based on the structure of the autoinducer of bacterial quorum sensing, N-(3-oxo-hexanoyl)-l-homoserine lactone (3-oxo-C6-HSL). Eight ß-keto ester analogues were synthesised with good yields and were spectroscopically characterised, showing that the compounds were only present in their ß-keto ester tautomer form. We carried out a computational analysis of the reactivity and ADME (absorption, distribution, metabolism, and excretion) properties of the compounds as well as molecular docking and molecular dynamics calculations with the LasR and LuxS quorum-sensing (QS) proteins, which are involved in bacterial resistance to antibiotics. The results show that all the compounds exhibit reliable ADME properties and that only compound 7 can present electrophile toxicity. The theoretical reactivity study shows that compounds 6 and 8 present a differential local reactivity regarding the rest of the series. Compound 8 presents the most promising potential in terms of its ability to interact with the LasR and LuxS QS proteins efficiently according to its molecular docking and molecular dynamics calculations. An initial in vitro antimicrobial screening was performed against the human pathogenic bacteria Pseudomonas aeruginosa and Staphylococcus aureus as well as the phytopathogenic bacteria Pseudomonas syringae and Agrobacterium tumefaciens. Compounds 6 and 8 exhibit the most promising results in the in vitro antimicrobial screening against the panel of bacteria studied.

6.
Biology (Basel) ; 12(7)2023 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-37508351

RESUMO

Endemic medicinal plants that grow at altitudes in northern Chile have been traditionally used for therapeutic applications by Aymara doctors. Several studies have analyzed the biological properties of these plants for therapeutic purposes. The aim was to characterize at molecular and biochemical levels the bacteria that live in the rhizosphere and roots from endemic medicinal plants that grow between 3681-5104 m.a.s.l. in the province of Parinacota. Thirty-nine bacteria were isolated from nine medicinal plants under our laboratory conditions. These bacteria were characterized by Gram stain, hydrolase production, plant-growth promotion, anti-fungal and antibacterial activities, and 16S rDNA sequencing. A phylogenetic study revealed the presence of three major phyla, Actinomycetota (46.2%), Bacillota (43.6%), and Pseudomonadota (10.3%). The rhizobacteria strains associated with the Aymara medicinal plant exhibited several interesting biological activities, such as hydrolytic enzymes, plant-growth-promoting traits, and antibacterial and antifungal properties, indicating their potential for developing new bio-based products for agricultural or clinical applications. These results are promising and highlight the need to point toward the search for explanations of the bio-molecular basis of the therapeutic effects of medicinal plants.

7.
Rev Med Chil ; 140(12): 1554-61, 2012 Dec.
Artigo em Espanhol | MEDLINE | ID: mdl-23677228

RESUMO

BACKGROUND: The Postgraduate Hospital Education Environment Measure (PHEEM) questionnaire, is a valid and reliable instrument to measure the educational environment (EE) in postgraduate medical education. AIM: To evaluate the EE perceived by the residents of a postgraduate training program using the PHEEM. MATERIAL AND METHODS: The PHEEM was applied in 2010-2011 in 35 specialty programs. We calculated their individual results and compared means of both global and individual domain scores of the PHEEM, by gender, university of origin and nationality. Cronbach's alpha coefficients and D study (Generalizability theory) were performed for reliability. RESULTS: Three hundred eighteen residents were surveyed (75.7% of the total universe). The mean score of the PHEEM was 105.09 ± 22.46 (65.7% of the maximal score) which is considered a positive EE. The instrument is highly reliable (Cronbach's alpha = 0.934). The D study found that 15 subjects are required to obtain reliable results (G coefficient = 0.813). There were no significant differences between gender and university of origin. Foreigners evaluated better the EE than Chileans and racism was not perceived. The programs showed a safe physical environment and teachers with good clinical skills. The negative aspects perceived were a lack of information about working hours, insufficient academic counseling, and scanty time left for extracurricular activities. CONCLUSIONS: This questionnaire allowed us to identify positive aspects of the EE, and areas to be improved in the specialty programs. The PHEEM is a useful instrument to evaluate the EE in Spanish-speaking participants of medical specialty programs.


Assuntos
Educação de Pós-Graduação em Medicina/normas , Meio Social , Inquéritos e Questionários/normas , Brasil , Feminino , Humanos , Masculino , Reprodutibilidade dos Testes
8.
HardwareX ; 9: e00117, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35492039

RESUMO

Realistic humanoid robots have emerged in the last two decades but the emotional intelligence of these machines has been limited. To teach humanoids how to emotionally communicate with humans, researchers have been increasingly relying on machine learning algorithms. While the software used to implement machine learning algorithms is largely open source, facially expressive humanoid robots are expensive and inaccessible to most people, thus limiting the number of researchers in this field. This paper aims to aid potential artificial intelligence researchers by providing a relatively inexpensive, open-source robot that can serve as a platform for research into emotional communication between humans and machines. Eva, the robot described in this paper, is an adult-sized humanoid head that can emulate human facial expressions, head movements, and speech through the use of 25 muscles, including 12 facial muscles that can produce a maximum skin displacement of 15 mm.

9.
Plants (Basel) ; 10(8)2021 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-34451576

RESUMO

Oregano from Socoroma (Atacama Desert) is characterized by its unique organoleptic properties and distinctive flavor and it is produced using ancestral pesticide-free agricultural practices performed by the Aymara communities. The cultivation in this zone is carried out under extreme conditions where the standard production of different crops is limited by several environmental factors, including aridity, high concentration of salts, and boron among others. However, oregano plants are associated with microorganisms that mitigate biotic and abiotic stresses present in this site. In this work, the S57 strain (member of the Pseudomonas genus that is closely related to Pseudomonas lini) was isolated from roots of oregano plants, which are grown in soils with high content of non-sodium salts and aluminum. This bacterium stimulates the growth of Micro-Tom tomato plants irrigated with saline-boric water. Moreover, it controls the growth of phytopathogenic fungi Fusarium oxysporum and Botrytis cinerea and the nematode Meloidogyne incognita under saline-boric conditions. Together with the high levels of bacterial biomass (~47 g/L), these results allow the establishment of the bases for developing a potential new agricultural bioproduct useful for arid and semiarid environments where commercial biological products show erratic behavior.

10.
Parasitol Res ; 106(4): 995-1000, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20165877

RESUMO

Diphyllobothriasis caused by the infection of adult Diphyllobothrium tapeworms sporadically occurs in Chile. The occurrence of the disease is closely linked to the consumption of raw or undercooked freshwater and marine fishes. Diagnosis of diphyllobothriasis has been based on laboratory examinations of the morphological characteristics of proglottids and eggs passed in the feces. Although determination of the parasite to the species level is possible through histologic examination of proglottid specimens, the parasites of patients who only discharge eggs cannot be diagnosed to the species level. Determining the species responsible for the infection of humans and other animals in affected areas is an important component of understanding the epidemiologic and enzootic characteristics of any infectious disease. We therefore compared the classification results obtained using a molecular approach with those obtained from morphological and histopathological examination of proglottids or eggs from five Chilean individuals with diphyllobothriasis. DNA analysis confirmed that the causative Diphyllobothrium species in Chile were first identified as Diphyllobothrium latum and Diphyllobothrium pacificum at least. Furthermore, mitochondrial cytochrome c oxidase subunit 1 gene analysis also supported the hypothesis that D. latum from Chile originated from Europe.


Assuntos
Difilobotríase/parasitologia , Diphyllobothrium/classificação , Diphyllobothrium/isolamento & purificação , Adolescente , Adulto , Animais , Pré-Escolar , Chile , DNA de Helmintos/química , DNA de Helmintos/genética , Diphyllobothrium/anatomia & histologia , Diphyllobothrium/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Feminino , Proteínas de Helminto/genética , Humanos , Masculino , Proteínas Mitocondriais/genética , Epidemiologia Molecular , Dados de Sequência Molecular , Análise de Sequência de DNA , Adulto Jovem
11.
J Parasitol ; 105(2): 263-270, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30945987

RESUMO

Dichelyne ( Cucullanellus) dichelyneformis ( Szidat, 1950 ) Petter, 1974 , was collected in the intestine of the rock cod, Eleginops maclovinus, in the Bay of Corral, Chile, and redescribed based on light and scanning electron microscopy. The correct arrangement of caudal papillae and post deirids, the shape of gubernaculum, and the morphology and number of denticles surrounding the oral opening are described for the first time. These characteristics along with the excretory pore position, size of spicules, and presence of a nodular thickening near the distal end of the spicules differentiate D. ( C.) dichelyneformis from the other congeners reported in South America and in the Antarctic. The new congeneric species found in E. maclovinus differs from D. ( C.) dichelyneformis based on the larger males, larger and more numerous denticles with a smooth surface, weakly developed sclerotized plates in esophastome, excretory pore and deirids posterior to nerve ring, shorter gubernaculum and spicules with no thickening on their distal region, lower spicules/body length ratio (10.4-15.1%), and a papilla-like phasmids closer to pairs 8 and 10.


Assuntos
Doenças dos Peixes/parasitologia , Perciformes/parasitologia , Spirurina/classificação , Animais , Baías , Chile , Feminino , Masculino , Microscopia Eletrônica de Varredura/veterinária , Spirurina/anatomia & histologia , Spirurina/isolamento & purificação , Spirurina/ultraestrutura
12.
Rev Inst Med Trop Sao Paulo ; 58: 62, 2016 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-27680167

RESUMO

The frequency of anti-Toxocara spp. antibodies in individuals attended by the Centro de Salud Familiar in the coastal Niebla town, Chile, was related to the host and to environmental factors. IgG anti- Toxocara antibodies were detected with a commercial ELISA kit (SCIMEDX Corporation, USA). Samples with undetermined absorbance values were subjected to an additional ELISA standardized by the Instituto de Salud Pública, Chilean Health Ministry, a commercial ELISA (NOVATEC, Germany), and a commercial Western blot kit (LDBio Diagnostics, France). Hematological exams were performed using an automated blood counter and blood smears. Dog feces were collected from the ground along the main road in Niebla, including rural and urban locations. Ninety (25.4%) of the 355 examined individuals were positive by the ELISA test. The frequency of anti-Toxocara antibodies and the infection risk were significantly higher (p < 0.05) among those individuals ≥ 40 years old with respect to the 20-39 years old group, in individuals from rural locations, those who did not have a safe drinking water supply in the house or who presented blood eosinophilia. The proportion of positive samples of dog feces and the mean number of Toxocara canis eggs/g of feces in urban and rural areas were similar (p > 0.05).

13.
Rev. Méd. Clín. Condes ; 32(5): 535-542, sept.-oct. 2021. tab
Artigo em Espanhol | LILACS | ID: biblio-1526030

RESUMO

Los trastornos del sueño son frecuentes en la población y una causa importante de morbilidad. El objetivo de esta revisión es evaluar las alteraciones del sueño en periodos de emergencia y desastres. A lo largo de la historia, la esfera biopsicosocial y el sueño de las personas ha sido abrumada por múltiples eventos a gran escala, tales como desastres naturales, tragedias provocadas por el hombre, conflictos bélicos, crisis sociales y pandemias, cuya experiencia puede derivar en problemas de salud a corto, mediano y/o largo plazo. En los estudios analizados, se ha observado el impacto negativo de las emergencias y desastres en el sueño, por lo que ha cobrado gran relevancia la difusión y promoción de medidas que incentiven el buen dormir. Debido a la llegada del COVID-19 y a la situación de confinamiento por periodos prolongados en el hogar para prevenir su propagación, han surgido importantes consecuencias a nivel social. Ciertos factores ocupacionales y características de los desastres se asocian a mayor comorbilidad, un alto riesgo de experimentar agotamiento físico, trastornos psicológicos e insomnio en grupos altamente vulnerables, como lo son los profesionales de la salud, rescatistas y socorristas. El insomnio es el trastorno de sueño más frecuente en la población general y su empeoramiento en el contexto de pandemia por COVID-19 representa un nuevo problema en salud pública. Es por ello, que es indispensable promover campañas de prevención de salud física y mental orientados a la pesquisa precoz y manejo de patologías de la esfera psicosocial, dentro de las posibilidades socioeconómicas.


Sleep disorders are common in the population and are major cause of morbidity. The objective of this review is to assess sleep disturbances in times of emergency and disasters. Throughout history, the biopsychosocial field and sleep have been affected by multiple large-scale events, such as natural disasters, man-caused tragedies, armed conflicts, social crises and pandemics, the experience of which can lead to short, medium and/or long term health problems. In several studies, the negative impact of emergencies and disasters on sleep have been analyzed, emphasizing the importance of the diffusion and promotion of measures that encourage good sleep. The arrival of COVID-19 and consequent home confinement for prolonged periods caused important social consequences. Certain occupational factors and characteristics of disasters are associated with greater comorbidity: a high risk of experiencing physical exhaustion, psychological disorders and insomnia, especially in highly vulnerable groups, such as health professionals, rescuers and first aids-responders. Insomnia is the most frequent sleep disorder in the general population and its worsening in the context of the COVID-19 pandemic, represents a new public health problem. It is essential to promote physical and mental health prevention campaigns, aimed at early screening and management of pathologies in the psychosocial sphere, within socioeconomic possibilities.


Assuntos
Humanos , Transtornos do Sono-Vigília/diagnóstico , Transtornos do Sono-Vigília/psicologia , Transtornos do Sono-Vigília/prevenção & controle , Emergências em Desastres , Quarentena , Pessoal de Saúde/psicologia , Desastres , Pandemias , Socorristas/psicologia , COVID-19 , Distúrbios do Início e da Manutenção do Sono/classificação , Distúrbios do Início e da Manutenção do Sono/diagnóstico
14.
J Parasitol ; 91(3): 707-9, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16108574

RESUMO

The present study was conducted to determine the impact of a caged-trout farm on the helminth parasites of the wild fish puyen chico (Galaxias maculatus) in Lake Moreno, southern Argentina. Samples of water, fish (G. maculatus), and snails (Heleobia hatcheri) were taken at 4 sampling stations in January 2001. Wild fish were parasitized by 8 helminth species, all of which are endemic in the region; therefore, the farm did not introduce any helminth parasite to G. maculatus. Fish captured near the farm were not infected by the digenean Steganoderma szidati, whereas the abundance of the digenean Acanthostomoides apophalliformis in these fish was significantly lower than that in fish captured away from the farm. This lower abundance may be explained by the absence in this area of the snail H. hatcheri, the parasite's first intermediate host, because of the effect of sediments and ammonium produced by the farm. To our knowledge, this is the first study in the Americas linking fish-farm pollution to helminth parasites in wild fish.


Assuntos
Doenças dos Peixes/epidemiologia , Pesqueiros , Helmintíase Animal/epidemiologia , Osmeriformes/parasitologia , Caramujos/crescimento & desenvolvimento , Animais , Animais Selvagens/parasitologia , Argentina/epidemiologia , Meio Ambiente , Doenças dos Peixes/parasitologia , Água Doce/química , Helmintíase Animal/parasitologia , Prevalência , Truta/parasitologia
15.
Phytochemistry ; 65(13): 1963-75, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15280003

RESUMO

The methanol extract from the aerial parts of Roldana barba-johannis (Asteraceae) afforded sargachromenol, sargahydroquinoic acid, and sargaquinoic acid. These natural products and their corresponding acetylated and methylated derivatives showed insecticidal and insect growth regulatory activities against the Fall Armyworm [Spodoptera frugiperda J.E. Smith, (Lepidoptera: Noctuidae)], an important insect pest of corn. The most active compounds were sargachromenol and its acetylated derivative; sargahydroquinoic acid and its acetylated derivative; and a mixture of sargachromenol, sargahydroquinoic acid, and sargaquinoic acid (6:3:1) and the acetylated form of this mixture. All these compounds and mixtures had significant inhibitory effects between 5.0 and 20.0 ppm in diets. Most compounds were insecticidal to larvae, with lethal doses between 20 and 35 ppm. In addition, these substances also demonstrated scavenging properties toward 2,2-diphenyl-1-picrylhydrazyl radical in TLC autographic and spectrophotometric assays. These compounds appear to have selective effects on the pre-emergence metabolism of the insect. The results from these compounds were fully comparable in activity to those known natural insect growth inhibitors such as gedunin and methanol extracts of Cedrela salvadorensis and Yucca periculosa. These substances may be useful as natural insecticidal agents.


Assuntos
Asteraceae/química , Hidroquinonas/farmacologia , Inseticidas/farmacologia , Spodoptera/efeitos dos fármacos , Tocotrienóis/farmacologia , Animais , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/farmacologia , Spodoptera/crescimento & desenvolvimento
16.
Phytochemistry ; 64(2): 463-73, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12943764

RESUMO

The methanol extract from the bark of Yucca periculosa F. Baker afforded 4,4'-dihydroxstilbene, resveratrol and 3,3',5,5'-tetrahydroxy-4-methoxystilbene and had growth regulatory activity against the Fall Army worm (Spodoptera frugiperda J.E. Smith, Lepidoptera:Noctuidae) an insect pest of corn. The most active compound was 3,3',5,5'-tetrahydroxy-4-methoxystilbene which had significant effects at 3 microg/g in diets. In addition to the inhibitory activity on bleaching of crocin induced by alkoxyl radicals, these compounds also demonstrated scavenging properties toward 2,2-diphenyl-1-picrylhydrazyl in TLC autographic and spectrophotometric assays. Our results indicate that these compounds could be involved in interference of sclerotization and moulting. These compounds appear to have selective effects on the pre-emergence metabolism of the insect. The results were fully comparable to known natural insect growth inhibitors such as gedunin and Cedrela extracts and have had a possible role as natural insecticidal agents.


Assuntos
Antioxidantes/farmacologia , Lepidópteros/crescimento & desenvolvimento , Controle Biológico de Vetores , Extratos Vegetais/química , Spodoptera/crescimento & desenvolvimento , Estilbenos/farmacologia , Yucca/química , Animais , Antioxidantes/isolamento & purificação , Azadirachta/química , Bioensaio/métodos , Compostos de Bifenilo , Carotenoides/química , Cedrela/toxicidade , Relação Dose-Resposta a Droga , Radicais Livres/antagonistas & inibidores , Larva/efeitos dos fármacos , Larva/crescimento & desenvolvimento , Lepidópteros/fisiologia , Limoninas/farmacologia , Picratos/antagonistas & inibidores , Casca de Planta/química , Pupa/efeitos dos fármacos , Pupa/crescimento & desenvolvimento , Spodoptera/fisiologia , Estilbenos/química , Estilbenos/isolamento & purificação
17.
Folia Parasitol (Praha) ; 51(1): 55-60, 2004 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15139378

RESUMO

Hysterothylacium winteri sp. n. (Nematoda: Anisakidae) was collected from the intestine of a marine-estuarine fish, Eleginops maclovinus (Valenciennes) (Perciformes: Eleginopsidae), from Abtao in the Gulf of Ancud, Chile. Sixteen (51.6%) out of 31 fish were infected; the intensity was 1-10 (mean 4) worms/host. The new species belongs to the group of congeners possessing one double pair of postanal papillae. By possessing a lateral pair of phasmids situated near the tip of tail, H. winteri most closely resembles Hysterothylacium habena. The new species can be distinguished by the lip flanges forming broadly rounded points and the equal, short spicules (320-400 microm long) representing 0.9-1.7% of body length.


Assuntos
Nematoides/anatomia & histologia , Perciformes/parasitologia , Animais , Chile , Feminino , Intestinos/parasitologia , Masculino , Microscopia Eletrônica de Varredura , Nematoides/ultraestrutura
18.
J Food Prot ; 77(7): 1237-40, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24988037

RESUMO

The objective of this research was to determine the occurrence of anisakid nematode larvae in hake ceviche sold in restaurants in Valdivia (39°48'S, 73°14'W) and Niebla (39°49'S, 73°22'W), Chile. Between August and November 2012, 78 portions of ceviche were collected (6 from each of the 13 restaurants that sell this product). Each portion was weighed and divided into approximately 30-g samples, which were placed in petri dishes with 0.15 M NaCl. All samples were manually shredded and then examined with a stereomicroscope. Muscles of 41 southern hake (Merluccius australis), a fish sold fresh in Valdivia, also were examined by candling to determine the presence of anisakid larvae. The presence of Pseudoterranova larvae in ceviche sold in Chile was identified for the first time. The pH of ceviche ranges from 4.1 to 4.8, which favors the presence of viable anisakid larvae that tolerate the acid pH similar to that found in the stomach of their mammalian host. No significant differences (P > 0.05) in the prevalence, mean abundance, and mean density of anisakid larvae in ceviche were found between localities. Larvae were detected in ceviche from 3 of 6 restaurants in Valdivia and 4 of 7 restaurants in Niebla. Of the 78 examined portions of ceviche, 21.8% had larvae. The prevalence of viable larvae was 16.7 and 7.1% in the examined portions from Valdivia and Niebla, respectively. In the 41 hake muscle samples from Valdivia, the prevalence (4.9%), mean abundance (0.1), and mean density (0.03) was the same for Pseudoterranova and Anisakis larvae. No inspection processes or pretreatments are currently in place for raw fish to achieve safe conditions for ceviche in restaurants from Valdivia and Niebla.


Assuntos
Anisakis/isolamento & purificação , Gadiformes/parasitologia , Alimentos Marinhos/parasitologia , Animais , Anisakis/crescimento & desenvolvimento , Chile , Contaminação de Alimentos/análise , Contaminação de Alimentos/economia , Larva/crescimento & desenvolvimento , Músculos/parasitologia , Alimentos Marinhos/economia
19.
J Wildl Dis ; 48(4): 937-50, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23060495

RESUMO

Diphyllobothriosis in fish from freshwater ecosystems in southern Chile was first reported in 1949. Infection by plerocercoids of Diphyllobothrium latum and Diphyllobothrium dendriticum occurs in introduced trout (Oncorhynchus mykiss) and native fish. We determined the prevalence, mean intensity, and mean abundance of seasonal infection and tissue damage produced by Diphyllobothrium spp. in native fish (Percichthys trucha, Odontesthes mauleanum, and Basilichthys australis) and introduced trout (O. mykiss) from Lake Panguipulli, Chile. Prevalence, mean intensity, and mean abundance of D. latum infection were significantly greater in trout than they were in native fish. Prevalence and mean abundance were similar in O. mauleanum and P. trucha, but they were greater than those in B. australis. Prevalence and abundance were similar among seasons between sexes for the four hosts. For all host species, except P. trucha, there was a statistically significant positive correlation between host length and the abundance of plerocercoids. Infections in muscle tissue were present in 61% of trout compared with 23% in O. mauleanum and 12% in P. trucha, suggesting a greater risk for human infection when consuming trout. In general, prevalence of infection by D. dendriticum was lower than was D. latum prevalence. Encapsulation of plerocercoids was common and severe in 71% of the trout examined. Only slight encapsulation of plerocercoids was found in the native O. mauleanum, and no encapsulation was observed in P. trucha or B. australis. The greater concentration of plerocercoids in the walls of the digestive tract of trout suggests a more-rapid immune response in trout than in native fish. The low frequency of encapsulation of plerocercoids in native fish would mean greater tissue damage in the natives than that observed in the trout because they are free to migrate among the viscera, potentially endangering these native fish populations in regions where Diphyllobothrium spp. are endemic.


Assuntos
Difilobotríase/veterinária , Doenças dos Peixes/epidemiologia , Truta , Animais , Chile/epidemiologia , Difilobotríase/epidemiologia , Difilobotríase/parasitologia , Difilobotríase/patologia , Feminino , Doenças dos Peixes/parasitologia , Doenças dos Peixes/patologia , Masculino , Prevalência , Estações do Ano , Truta/parasitologia
20.
Actual. psicol. (Impr.) ; 31(122)jun. 2017.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1505583

RESUMO

Esta investigación tiene el propósito de evaluar y comparar a personas heterosexuales y homosexuales, con relaciones de pareja estables, respecto a los componentes de la teoría triangular del amor, tácticas de resolución de conflictos, ajuste con la pareja y bienestar psicológico. Participaron 157 adultos jóvenes chilenos, 77 homosexuales (30 lesbianas y 47 gays) y 80 heterosexuales (45 mujeres y 35 hombres). Se utilizaron la Escala de Ajuste Diádico, la Escala Triangular del Amor de Sternberg, la Flourishing Scale de Diener y el Conflict Resolution Style Inventory de Kurdek. Los resultados muestran algunas diferencias significativas en tácticas de resolución de conflicto, componentes del amor y ajuste en la pareja, según la orientación sexual; además existen diferencias entre las mujeres que conviven y no conviven.


The aim of this study was to evaluate and compare heterosexual and homosexual people who are in stable relationships, with respect to the components of the triangular theory of love: conflict resolution tactics, couple adjustment and psychological wellbeing. The participants were comosed of 157 young adults Chileans: 77 homosexuals (30 lesbians and 47 gay men) and 80 heterosexuals (45 women and 35 men). The instruments used were the Dyadic Adjustment Scale, the Sternberg's Triangular Love Scale, the Flourishing Scale of Diener and Conflict Resolution Style Inventory of Kurdek. The results show that there are significant differences in some conflict resolution tactics, components of love between partners, and couple adjustment according to sexual orientation. In addition, there are also differences between women living and not living with a partner.

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