Nanoarchitectonics Design Strategy of Metal-Organic Framework and Bio-Metal-Organic Framework Composites for Advanced Wastewater Treatment through Adsorption.

Freshwater depletion is an alarm for finding an eco-friendly solution to treat wastewater for drinking and domestic applications. Though several methods like chlorination, filtration, and coagulation-sedimentation are conventionally employed for water treatment, these methods need to be improved as they are not environmentally friendly, rely on chemicals, and are ineffective for all kinds of pollutants. These problems can be addressed by employing an alternative solution that is effective for efficient water treatment and favors commercial aspects. Metal organic frameworks (MOFs), an emerging porous material, possess high stability, pore size tunability, greater surface area, and active sites. These MOFs can be tailored; thus, they can be customized according to the target pollutant. Hence, MOFs can be employed as adsorbents that effectively target different pollutants. Bio-MOFs are a kind of MOFs that are incorporated with biomolecules, which also possess properties of MOFs and are used as a nontoxic adsorbent. In this review, we elaborate on the interaction between MOFs and target pollutants, the role of linkers in the adsorption of contaminants, tailoring strategy that can be employed on MOFs and Bio-MOFs to target specific pollutants, and we also highlight the effect of environmental matrices on adsorption of pollutants by MOFs.

Biosequestration of lignin in municipal landfill leachate by tailored cationic lipoprotein biosurfactant through Bacillus tropicus valorized tannery solid waste.

Bioremediation of municipal landfill leachate (MLL) is often intricate due to presence of refractory lignin. In the present study, it was attempted to tailor the histidine rich protein moiety of cationic lipoprotein biosurfactant (CLB) to sequester the lignin from MLL. Animal fleshing (AF), the solid waste generated in tanning industry was utilized for the production of histidine rich CLB by de novo substrate dependent synthesis pathway involving Bacillus tropicus. The optimum conditions for the maximum production of CLB were determined using response surface methodology. At the optimized conditions, the maximum yield of CLB was 217.4 mg/g AF (on dry basis). The produced histidine rich CLB was purified using Immobilized metal affinity chromatography at the optimum binding and elution conditions. The histidine residues were more pronounced in the CLB, as determined by HPLC analysis. The CLB was further characterized by SDS-PAGE, Zeta potential, XRD, FT-IR, Raman, NMR, GC-MS and TG analyses. The CLB was immobilized onto functionalized nanoporous activated bio carbon (FNABC) and the optimum immobilization capacity was found to be 211.6 mg/g FNABC. The immobilization of CLB onto FNABC was confirmed using SEM, FT-IR, XRD and TG analyses. The isotherm models, kinetic and thermodynamics studies of CLB immobilization onto FNABC were performed to evaluate its field level application. Subsequently, the CLB-FNABC was then applied for the sequestration of lignin in MLL. The maximum lignin sequestration was achieved by 92.5 mg/g CLB-FNABC at the optimized sequestration time, 180 min; pH, 5; temperature, 45 °C and mass of CLB-FNABC, 1.0 g. The sequestration of lignin by CLB- FNABC was confirmed by SEM, FT-IR and UV-Vis analyses. Further, the mechanistic study revealed the anchoring of CLB onto the surface of lignin through electrostatic interaction.

Laccase driven biocatalytic oxidation to reduce polymeric surface hydrophobicity: An effective pre-treatment strategy to enhance biofilm mediated degradation of polyethylene and polycarbonate plastics.

Plastic pollution is a major global environmental issue due to its structural complexity and poor biodegradability. Biological approaches are appropriate due to cost effectiveness and environmental friendliness, however effective polymer degradation is still in its infancy. As biological treatments are slower than physical and chemical approaches, they could be applied in conjunction with pre-treatment techniques such as photo-oxidation, heat treatment, and chemical treatments. But these processes lead to high energy consumption and hazardous secondary pollution. To address these concerns, an enzymatic pre-treatment strategy has been proposed in this study, with an aim of promoting surface oxidation on the plastics leading to improved hydrophilicity. This in turn, facilitates the surface attachment of microbes, ultimately, accelerating biodegradation. Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FT-IR) spectroscopy analyses confirmed the surface oxidation of the polyethylene (PE) and polycarbonate (PC) plastics mediated by the action of laccase enzyme. Contact angle measurement witnessed the increased hydrophilicity of the treated plastics. Following, a potential biofilm forming microbial consortium has been employed for the biodegradation of enzyme treated plastics. SEM analysis indicated the increased formation of corrosive pits and surface aberrations on the enzymatically pre-treated plastics and Confocal Laser Scanning microscopy (CLSM) analysis exhibited the enhanced biofilm formation and exopolysaccharide deposition on the pre-treated PE and PC. In addition, X-ray photoelectron spectroscopy (XPS) revealed the reduction in the elemental composition of carbon with an increment in the oxygen composition of plastics. Gel permeation chromatography (GPC) further confirmed the greater reduction in the molecular weights of the plastics subjected to integrated enzymatic and biofilm treatment than only biofilm treated plastics. This is the first report on the integration of enzymatic pre-treatment with the biofilm mediated microbial degradation to achieve enhanced treatment of plastics which demonstrated to be a promising technology for the effective mitigation of plastic pollution.

Supramolecular bioamphiphile facilitated bioemulsification and concomitant treatment of recalcitrant hydrocarbons in petroleum refining industry oily waste.

Bioremediation of real-time petroleum refining industry oily waste (PRIOW) is a major challenge due to the poor emulsification potential and oil sludge disintegration efficiency of conventional bioamphiphile molecules. The present study was focused on the design of a covalently engineered supramolecular bioamphiphile complex (SUBC) rich in hydrophobic amino acids for proficient emulsification of hydrocarbons followed by the concomitant degradation of total petroleum hydrocarbons (TPH) in PRIOW using the hydrocarbonoclastic microbial bio-formulation system. The synthesis of SUBC was carried out by pH regulated microbial biosynthesis process and the yield was obtained to be 450.8 mg/g of petroleum oil sludge. The FT-IR and XPS analyses of SUBC revealed the anchoring of hydrophilic moieties of monomeric bioamphiphilic molecules, resulting in the formation of SUBC via covalent interaction. The SUBC was found to be lipoprotein in nature. The maximum loading capacity of SUBC onto surface modified rice hull (SMRH) was achieved to be 45.25 mg/g SMRH at the optimized conditions using RSM-CCD design. The SUBC anchored SMRH was confirmed using SEM, FT-IR, XRD and TGA analyses. The adsorption isotherm models of SUBC onto SMRH were performed. The integrated approach of SUBC-SMRH and hydrocarbonoclastic microbial bio-formulation system, emulsified oil from PRIOW by 92.86 ± 2.26% within 24 h and degraded TPH by 89.25 ± 1.75% within 4 days at the optimum dosage ratio of SUBC-SMRH (0.25 g): PRIOW (1 g): mass of microbial-assisted biocarrier material (0.05 g). The TPH degradation was confirmed by SARA fractional analysis, FT-IR, 1H NMR and GC-MS analyses. The study suggested that the application of covalently engineered SUBC has resulted in the accelerated degradation of real-time PRIOW in a very short duration without any secondary sludge generation. Thus, the SUBC integrated approach can be considered to effectively manage the hydrocarbon contaminants from petroleum refining industries under optimal conditions.