Growth hormone-releasing peptide 6 prevents cutaneous hypertrophic scarring: early mechanistic data from a proteome study.

Hypertrophic scars (HTS) and keloids are forms of aberrant cutaneous healing with excessive extracellular matrix (ECM) deposition. Current therapies still fall short and cause undesired effects. We aimed to thoroughly evaluate the ability of growth hormone releasing peptide 6 (GHRP6) to both prevent and reverse cutaneous fibrosis and to acquire the earliest proteome data supporting GHRP6's acute impact on aesthetic wound healing. Two independent sets of experiments addressing prevention and reversion effects were conducted on the classic HTS model in rabbits. In the prevention approach, the wounds were assigned to topically receive GHRP6, triamcinolone acetonide (TA), or vehicle (1% sodium carboxy methylcellulose [CMC]) from day 1 to day 30 post-wounding. The reversion scheme was based on the infiltration of either GHRP6 or sterile saline in mature HTS for 4 consecutive weeks. The incidence and appearance of HTS were systematically monitored. The sub-epidermal fibrotic core area of HTS was ultrasonographically determined, and the scar elevation index was calculated on haematoxylin/eosin-stained, microscopic digitised images. Tissue samples were collected for proteomics after 1 hour of HTS induction and treatment with either GHRP6 or vehicle. GHRP6 prevented the onset of HTS without the untoward reactions induced by the first-line treatment triamcinolone acetonide (TA); however, it failed to significantly reverse mature HTS. The preliminary proteomic study suggests that the anti-fibrotic preventing effect exerted by GHRP6 depends on different pathways involved in lipid metabolism, cytoskeleton arrangements, epidermal cells' differentiation, and ECM dynamics. These results enlighten the potential success of GHRP6 as one of the incoming alternatives for HTS prevention.

Uso de la electroencefalografía en la identificación de lesiones vasculares cerebrales asintomáticas en hipertensos / Use of electroencephalography to identify asymptomatic cerebrovascular lesions among hypertensives

OBJETIVOS: en este trabajo nos propusimos identificar la relación entre las alteraciones electroencefalográficas y las lesiones vasculares cerebrales iniciales en pacientes hipertensos neurológicamente asintomáticos, con el uso de la escala GTE. MÉTODOS: se obtuvieron imágenes de resonancia magnética (0.35 Tesla, T1,T2, FLAIR) y electroencefalograma de 19 canales en 49 hipertensos asintomáticos, los cuales fueron divididos en 3 grupos: A, sin lesiones cerebrovasculares; B, con lesión vascular cerebral no clásica y C, con lesión cerebrovascular isquémica clásica. RESULTADOS: el grupo C mostró valores promedios superiores de la escala GTE (4,42) que el grupo B (3,36) y A (2,63) con una diferencia significativa de la mediana alcanzada por cada grupo (p=0,03). CONCLUSIÓN: las alteraciones electroencefalográficas en los pacientes de nuestro estudio están asociadas con los cambios cerebrovasculares inducidos por la hipertensión arterial. La escala GTE es un método simple que podría ser empleado en el pesquisaje de lesión cerebrovascular subclínica en pacientes hipertensos.

OBJECTIVES: Identify the relationship between electroencephalographic alterations and initial cerebrovascular lesions among neurologically asymptomatic hypertensive patients using the GTE scale. METHODS: Magnetic resonance (0.35 Tesla, T1, T2, FLAIR) and 19-channel electroencephalogram images were obtained from 49 asymptomatic hypertensives, who were divided into three groups: A, without cerebrovascular lesion; B, with non-classical cerebrovascular lesion; and C, with classical ischemic cerebrovascular lesion. RESULTS: Group C showed higher mean values on the GTE scale (4.42) than Groups B (3.36) and A (2.63), with a significant difference in the median for each group (p=0.03). CONCLUSION: The electroencephalographic alterations found in the patients studied are associated with cerebrovascular changes induced by arterial hypertension. The GTE scale is a simple method which could be used for the screening of subclinical cerebrovascular lesions among hypertensive patients.