RESUMO
BACKGROUND & AIMS: Subsets of leukocytes synergize with regenerative growth factors to promote hepatic regeneration. γδT cells are early responders to inflammation-induced injury in a number of contexts. We investigated the role of γδT cells in hepatic regeneration using mice with disruptions in Tcrd (encodes the T-cell receptor δ chain) and Clec7a (encodes C-type lectin domain family 7 member a, also known as DECTIN1). METHODS: We performed partial hepatectomies on wild-type C57BL/6, CD45.1, Tcrd(-/-), or Clec7a(-/-) mice. Cells were isolated from livers of patients and mice via mechanical and enzymatic digestion. γδT cells were purified by fluorescence-activated cell sorting. RESULTS: In mice, partial hepatectomy up-regulated expression of CCL20 and ligands of Dectin-1, which was associated with recruitment and activation of γδT cells and their increased production of interleukin (IL)-17 family cytokines. Recruited γδT cells induced production of IL-6 by antigen-presenting cells and suppressed expression of interferon gamma by natural killer T cells, promoting hepatocyte proliferation. Absence of IL-17-producing γδT cells or deletion of Dectin-1 prevented development of regenerative phenotypes in subsets of innate immune cells. This slowed liver regeneration and was associated with reduced expression of regenerative growth factors and cell cycle regulators. Conversely, exogenous administration of IL-17 family cytokines or Dectin-1 ligands promoted regeneration. More broadly, we found that γδT cells are required for inflammatory responses mediated by IL-17 and Dectin-1. CONCLUSIONS: γδT cells regulate hepatic regeneration by producing IL-22 and IL-17, which have direct mitogenic effects on hepatocytes and promote a regenerative phenotype in hepatic leukocytes, respectively. Dectin-1 ligation is required for γδT cells to promote hepatic regeneration.
Assuntos
Proliferação de Células , Hepatócitos/metabolismo , Mediadores da Inflamação/metabolismo , Interleucina-17/metabolismo , Regeneração Hepática , Fígado/metabolismo , Receptores de Antígenos de Linfócitos T gama-delta/metabolismo , Linfócitos T/metabolismo , Animais , Células Cultivadas , Quimiocina CCL20/metabolismo , Genótipo , Hepatectomia , Hepatócitos/imunologia , Humanos , Interferon gama/metabolismo , Interleucina-6/metabolismo , Interleucinas/metabolismo , Lectinas Tipo C/deficiência , Lectinas Tipo C/genética , Lectinas Tipo C/metabolismo , Fígado/imunologia , Fígado/cirurgia , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fenótipo , Receptores de Antígenos de Linfócitos T gama-delta/deficiência , Receptores de Antígenos de Linfócitos T gama-delta/genética , Transdução de Sinais , Linfócitos T/imunologia , Fatores de Tempo , Interleucina 22RESUMO
Dectin-1 is a C-type lectin receptor critical in anti-fungal immunity, but Dectin-1 has not been linked to regulation of sterile inflammation or oncogenesis. We found that Dectin-1 expression is upregulated in hepatic fibrosis and liver cancer. However, Dectin-1 deletion exacerbates liver fibro-inflammatory disease and accelerates hepatocarcinogenesis. Mechanistically, we found that Dectin-1 protects against chronic liver disease by suppressing TLR4 signaling in hepatic inflammatory and stellate cells. Accordingly, Dectin-1(-/-) mice exhibited augmented cytokine production and reduced survival in lipopolysaccharide (LPS)-mediated sepsis, whereas Dectin-1 activation was protective. We showed that Dectin-1 inhibits TLR4 signaling by mitigating TLR4 and CD14 expression, which are regulated by Dectin-1-dependent macrophage colony stimulating factor (M-CSF) expression. Our study suggests that Dectin-1 is an attractive target for experimental therapeutics in hepatic fibrosis and neoplastic transformation. More broadly, our work deciphers critical cross-talk between pattern recognition receptors and implicates a role for Dectin-1 in suppression of sterile inflammation, inflammation-induced oncogenesis, and LPS-mediated sepsis.
Assuntos
Lectinas Tipo C/metabolismo , Cirrose Hepática/patologia , Neoplasias Hepáticas/patologia , Receptor 4 Toll-Like/metabolismo , Animais , Transformação Celular Neoplásica/efeitos dos fármacos , Células Cultivadas , Quimiocina CCL2/sangue , Citocinas/metabolismo , Dietilnitrosamina/toxicidade , Hepatócitos/citologia , Hepatócitos/metabolismo , Humanos , Inflamação , Lectinas Tipo C/deficiência , Lectinas Tipo C/genética , Receptores de Lipopolissacarídeos/metabolismo , Lipopolissacarídeos/toxicidade , Fígado/metabolismo , Fígado/patologia , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/metabolismo , Neoplasias Hepáticas/induzido quimicamente , Neoplasias Hepáticas/metabolismo , Fator Estimulador de Colônias de Macrófagos/genética , Fator Estimulador de Colônias de Macrófagos/metabolismo , Fator Estimulador de Colônias de Macrófagos/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/farmacologia , Sepse/etiologia , Transdução de Sinais/efeitos dos fármacos , Tioacetamida/toxicidade , Receptor 4 Toll-Like/antagonistas & inibidores , Regulação para Cima/efeitos dos fármacosRESUMO
Newborn rats chronically exposed to moderate hyperoxia (60% O2) exhibit abnormal respiratory control, including decreased eupneic ventilation. To further characterize this plasticity and explore its proximate mechanisms, rats were exposed to either 21% O2 (Control) or 60% O2 (Hyperoxia) from birth until studied at 3-14 days of age (P3-P14). Normoxic ventilation was reduced in Hyperoxia rats when studied at P3, P4, and P6-7 and this was reflected in diminished arterial O2 saturations; eupneic ventilation spontaneously recovered by P13-14 despite continuous hyperoxia, or within 24h when Hyperoxia rats were returned to room air. Normoxic metabolism was also reduced in Hyperoxia rats but could be increased by raising inspired O2 levels (to 60% O2) or by uncoupling oxidative phosphorylation within the mitochondrion (2,4-dinitrophenol). In contrast, moderate increases in inspired O2 had no effect on sustained ventilation which indicates that hypoventilation can be dissociated from hypometabolism. The ventilatory response to abrupt O2 inhalation was diminished in Hyperoxia rats at P4 and P6-7, consistent with smaller contributions of peripheral chemoreceptors to eupneic ventilation at these ages. Finally, the spontaneous respiratory rhythm generated in isolated brainstem-spinal cord preparations was significantly slower and more variable in P3-4 Hyperoxia rats than in age-matched Controls. We conclude that developmental hyperoxia impairs both peripheral and central components of eupneic ventilatory drive. Although developmental hyperoxia diminishes metabolism as well, this appears to be a regulated hypometabolism and contributes little to the observed changes in ventilation.
Assuntos
Tronco Encefálico/crescimento & desenvolvimento , Hiperóxia/fisiopatologia , Consumo de Oxigênio/fisiologia , Ventilação Pulmonar/fisiologia , Medula Espinal/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos , Relógios Biológicos/fisiologia , Análise Química do Sangue , Temperatura Corporal/fisiologia , Tronco Encefálico/fisiopatologia , Dióxido de Carbono/metabolismo , Células Quimiorreceptoras/fisiologia , Coração/crescimento & desenvolvimento , Frequência Cardíaca/fisiologia , Oxigênio/metabolismo , Fosforilação/fisiologia , Ratos Sprague-Dawley , Mecânica Respiratória/fisiologia , Medula Espinal/fisiopatologia , Técnicas de Cultura de TecidosRESUMO
The O(2) sensitivity of the neonatal rat carotid body is increased after 1 day in moderate hyperoxia (60% O(2)) (Donnelly et al., 2009). We investigated whether this enhanced peripheral chemosensitivity increases the hypoxic ventilatory response (HVR) and tested the hypothesis that this plasticity is mediated by the superoxide anion. Neonatal rats (7 d old) were injected with saline or MnTMPyP, a superoxide scavenger, and placed into 60% O(2) for 23-28h. Baseline ventilation was reduced and the acute HVR (12% O(2)) was enhanced in hyperoxia-treated rats relative to age-matched controls; MnTMPyP did not block these effects. An additional group of rats was studied after only 30min in 60% O(2). This shorter exposure had no effect on normoxic ventilation or the HVR. We conclude that 1 d, but not 30min, of 60% O(2) augments the HVR of neonatal rats and that production of the superoxide anion does not contribute to this plasticity.