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1.
Allergy ; 76(8): 2367-2382, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-33866585

RESUMO

Many allergens feature hydrophobic cavities that allow the binding of primarily hydrophobic small-molecule ligands. Ligand-binding specificities can be strict or promiscuous. Serum albumins from mammals and birds can assume multiple conformations that facilitate the binding of a broad spectrum of compounds. Pollen and plant food allergens of the family 10 of pathogenesis-related proteins bind a variety of small molecules such as glycosylated flavonoid derivatives, flavonoids, cytokinins, and steroids in vitro. However, their natural ligand binding was reported to be highly specific. Insect and mammalian lipocalins transport odorants, pheromones, catecholamines, and fatty acids with a similar level of specificity, while the food allergen ß-lactoglobulin from cow's milk is notably more promiscuous. Non-specific lipid transfer proteins from pollen and plant foods bind a wide variety of lipids, from phospholipids to fatty acids, as well as sterols and prostaglandin B2, aided by the high plasticity and flexibility displayed by their lipid-binding cavities. Ligands increase the stability of allergens to thermal and/or proteolytic degradation. They can also act as immunomodulatory agents that favor a Th2 polarization. In summary, ligand-binding allergens expose the immune system to a variety of biologically active compounds whose impact on the sensitization process has not been well studied thus far.


Assuntos
Alérgenos , Hipersensibilidade Alimentar , Alérgenos/metabolismo , Animais , Bovinos , Feminino , Ligantes , Pólen , Ligação Proteica
2.
Occup Environ Med ; 78(2): 142-144, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33067336

RESUMO

OBJECTIVES: Several studies have described peach tree (PT) as an occupational allergen. The aim of this work was to assess the effect of Prunus persica 9 (Pru p 9), a recently identified allergen from PT pollen, in exposed workers. METHODS: The study included people who reported respiratory symptoms after handling PT in orchards during the flowering period (Blanca village, Murcia region, south-east Spain). After completing a detailed questionnaire, participants underwent skin prick test (SPT) and nasal provocation test (NPT). The IgE response was analysed by SDS-PAGE and immunoblotting assays. RESULTS: A total of 21 cases were included (mean age 45 years; 57% women). Most were polysensitised to common pollens, although one person was sensitised only to PT pollen. All cases had a positive SPT to this pollen, and 43% also to Pru p 9. All participants reported having rhinitis, and six participants reported having also asthma. Immunoblotting showed a heterogeneous IgE pattern for several proteins, with Pru p 9 recognised in nine cases. Most participants sensitised to PT pollen and Pru p 9 had positive NPTs, while those who were not sensitised to Pru p 9 tested negative. CONCLUSIONS: We demonstrate for the first time that Pru p 9, an allergen from PT pollen, can induce respiratory symptoms following occupational exposure. This must be considered a relevant allergen when people working with PT cultivars develop respiratory symptoms.


Assuntos
Doenças dos Trabalhadores Agrícolas/imunologia , Asma Ocupacional/imunologia , Exposição Ocupacional/efeitos adversos , Pólen/imunologia , Prunus persica/imunologia , Rinite Alérgica Sazonal/imunologia , Testes de Provocação Brônquica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Testes Cutâneos , Espanha
3.
Allergy ; 75(4): 798-807, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31605380

RESUMO

BACKGROUND: Ole e 7 is a nonspecific lipid transfer protein (nsLTP) from olive pollen, one of the main allergenic pollens worldwide. This allergenic nsLTP is responsible for severe symptoms in regions with high olive pollen exposure, where many Ole e 7-sensitized patients exhibit a co-sensitization to the peach nsLTP, Pru p 3. However, there is no evidence of cross-reactivity, which explains this observed co-sensitization. Therefore, the purpose of this study was to explore the relationship between Ole e 7 and Pru p 3. METHODS: A total of 48 patients sensitized to Ole e 7 and/or Pru p 3 were included in the study. Specific IgE serum levels were measured by ImmunoCAP 250 and ELISA. Inhibition assays were performed to determine the existence of cross-reactivity between both nsLTPs. Allergic response was analyzed ex vivo (basophil activation test) and in vitro (RBL-2H3 mast cell model). RESULTS: Common IgG and IgE epitopes were identified between both allergens. IgE-binding inhibition was detected in Ole e 7-monosensitized patients using rPru p 3 as inhibitor, reaching inhibition values of 25 and 100%. Ex vivo and in vitro assays revealed a response against rPru p 3 in four (31%) Ole e 7-monosensitized patients. CONCLUSIONS: Our results suggest that Ole e 7 could play a new role as primary sensitizer in regions with high olive pollen exposure, leading to the peach nsLTP sensitization. This co-sensitization process would occur because of the cross-reactivity between Ole e 7 and Pru p 3 observed in some allergic patients.


Assuntos
Alérgenos , Antígenos de Plantas , Reações Cruzadas , Humanos , Imunoglobulina E , Lipídeos , Proteínas de Plantas , Pólen/imunologia
4.
Mol Pharm ; 17(3): 827-836, 2020 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-31990560

RESUMO

Olive pollen is one of the most important causes of respiratory allergy, with Ole e 1 being the most clinically relevant sensitizing allergen. Peptide-based vaccines represent promising therapeutic approaches, but the use of adjuvants is required to strengthen the weak immunogenicity of small peptides. We propose the use of dendrimeric scaffolds conjugated to the T cell immunodominant epitope of Ole e 1 (OE109-130) for the development of novel vaccines against olive pollen allergy. Four dendrimeric scaffolds containing an ester/ether with nine mannoses, an ester succinimidyl linker with nine N-acetyl-glucosamine units or nine ethylene glycol units conjugated to OE109-130 peptide were designed, and their cytotoxicity, internalization pattern, and immunomodulatory properties were analyzed in vitro. None of the dendrimers exhibited cytotoxicity in humanized rat basophil (RBL-2H3), human bronchial epithelial Calu-3, and human mast LAD2 cell lines. Confocal images indicated that mannosylated glycodendropeptides exhibited lower colocalization with a lysosomal marker. Moreover, mannosylated glycodendropeptides showed higher transport tendency through the epithelial barrier formed by Calu-3 cells cultured at the air-liquid interface. Finally, mannosylated glycodendropeptides promoted Treg and IL10+Treg proliferation and IL-10 secretion by peripheral blood mononuclear cells from allergic patients. Mannosylated dendrimers conjugated with OE109-130 peptide from Ole e 1 have been identified as suitable candidates for the development of novel vaccines of olive pollen allergy.


Assuntos
Antígenos de Plantas/química , Dendrímeros/química , Manose/imunologia , Olea/química , Olea/imunologia , Peptídeos/imunologia , Proteínas de Plantas/química , Pólen/imunologia , Rinite Alérgica Sazonal/prevenção & controle , Vacinas de Subunidades Antigênicas/imunologia , Adjuvantes Imunológicos/química , Animais , Antígenos de Plantas/imunologia , Linhagem Celular Tumoral , Sobrevivência Celular/imunologia , Citocinas/análise , Citocinas/metabolismo , Células Epiteliais/imunologia , Células Epiteliais/metabolismo , Epitopos de Linfócito T/química , Epitopos de Linfócito T/imunologia , Humanos , Imunogenicidade da Vacina , Manose/química , Peptídeos/química , Proteínas de Plantas/imunologia , Ratos , Mucosa Respiratória/imunologia , Mucosa Respiratória/metabolismo , Rinite Alérgica Sazonal/sangue , Rinite Alérgica Sazonal/imunologia , Linfócitos T/imunologia , Linfócitos T/metabolismo
5.
J Proteome Res ; 18(7): 2940-2953, 2019 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-31136180

RESUMO

The characterization of the humoral response in Alzheimer's disease (AD) patients might aid in detecting the disease at early stages. We have combined phage display and protein microarrays to identify AD autoantibodies and their target biomarkers. After enrichment of the T7 phage display libraries from AD and healthy brain tissue mRNA in AD-specific phages, 1536 monoclonal phages were printed on microarrays to probe them with 8 AD and 8 healthy control sera. A total of 57 phages showed higher seroreactivity in AD. In total, 13 out of the 44 unique sequences displayed on the phages were selected for validation using 68 AD and 52 healthy control sera. Peptides from Anthrax toxin receptor 1, Nuclear protein 1, Glycogen phosphorylase, and Olfactory receptor 8J1 expressed in bacteria as HaloTag fusion proteins showed a statistically significant ability to discriminate between AD patients and controls. The identified panel of AD autoantibodies might provide new insights into the blood-based diagnosis of the disease.


Assuntos
Doença de Alzheimer/imunologia , Autoanticorpos/análise , Doença de Alzheimer/diagnóstico , Bacteriófagos/imunologia , Biomarcadores/sangue , Estudos de Casos e Controles , Humanos , Peptídeos/genética , Análise Serial de Proteínas/métodos
6.
J Proteome Res ; 18(8): 3052-3066, 2019 08 02.
Artigo em Inglês | MEDLINE | ID: mdl-31192604

RESUMO

Olive pollen is a major allergenic source worldwide due to its extensive cultivation. We have combined available genomics data with a comprehensive proteomics approach to get the annotated olive tree (Olea europaea L.) pollen proteome and define its complex allergenome. A total of 1907 proteins were identified by LC-MS/MS using predicted protein sequences from its genome. Most proteins (60%) were predicted to possess catalytic activity and be involved in metabolic processes. In total, 203 proteins belonging to 47 allergen families were found in olive pollen. A peptidyl-prolyl cis-trans isomerase, cyclophilin, produced in Escherichia coli, was found as a new olive pollen allergen (Ole e 15). Most Ole e 15-sensitized patients were children (63%) and showed strong IgE recognition to the allergen. Ole e 15 shared high sequence identity with other plant, animal, and fungal cyclophilins and presented high IgE cross-reactivity with pollen, plant food, and animal extracts.


Assuntos
Alérgenos/genética , Antígenos de Plantas/genética , Ciclofilinas/genética , Ciclofilinas/imunologia , Proteoma/genética , Alérgenos/imunologia , Alérgenos/isolamento & purificação , Sequência de Aminoácidos/genética , Animais , Criança , Cromatografia Líquida , Reações Cruzadas , Humanos , Imunoglobulina E/genética , Imunoglobulina E/imunologia , Olea/efeitos adversos , Olea/genética , Olea/imunologia , Pólen/efeitos adversos , Pólen/genética , Pólen/imunologia , Proteoma/imunologia , Proteômica , Espectrometria de Massas em Tandem
7.
Int Arch Allergy Immunol ; 177(4): 290-301, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30173224

RESUMO

BACKGROUND: Cross-reactivity reactions between allergenic polygalacturonases (PGs) from different biological sources, especially foods and pollens from the Oleaceae family, have been described using Salsola kali PG (Sal k 6). No PG from olive pollen has been characterized to date, hampering further knowledge about cross-reactions through PGs. OBJECTIVES: The aim of this work was to determine the potential allergenicity of the PG from olive pollen and clarify its role in cross-reactivity. METHODS: A cDNA-encoding olive pollen PG sequence was subcloned into the pET41b vector and used to transform BL21(DE3) Escherichia coli cells to produce a His-tag fusion recombinant protein. The allergenic properties of olive pollen PG were determined by immunoblotting and ELISA in comparison to Sal k 6. The cross-reactivity potential of the protein with other pollen sources was analyzed by inhibition immunoassays. RESULTS: The existence of other isoforms of Ole e 14 with different allergenicity was confirmed by proteomics and a meta-analysis of the recently reported olive genome. Sal k 6 showed a higher IgE recognition than Ole e 14 regardless of patient sensitization, suggesting the existence of more allergenic Ole e 14 isoforms in olive pollen. IgG and IgE inhibition assays supported the existence of cross-reactions between them and with other PGs from Oleaceae and Poaceae plant families. CONCLUSIONS: A new allergen from olive pollen, Ole e 14, has been identified, produced as a recombinant isoform, and structurally and immunologically characterized. Its role in cross-reactivity has been confirmed and, due to its smaller IgE binding capacity, it could have an important role for therapeutic purposes.


Assuntos
Alérgenos/metabolismo , Antígenos de Plantas/metabolismo , Poligalacturonase/metabolismo , Rinite Alérgica Sazonal/imunologia , Alérgenos/genética , Alérgenos/imunologia , Sequência de Aminoácidos/genética , Antígenos de Plantas/genética , Western Blotting , Clonagem Molecular , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática , Imunoglobulina E/metabolismo , Olea/imunologia , Pólen/genética , Pólen/metabolismo , Poligalacturonase/genética , Isoformas de Proteínas/genética , Proteômica , Salsola/imunologia
8.
Biochim Biophys Acta Proteins Proteom ; 1865(8): 1067-1076, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28502749

RESUMO

A highly prevalent IgE-binding protein band of 28kDa is observed when Salsola kali pollen extract is incubated with individual sera from Amaranthaceae pollen sensitized patients. By an immunoproteomic analysis of S. kali pollen extract, we identified this protein band as an allergenic polygalacturonase enzyme. The allergen, named Sal k 6, exhibits a pI of 7.14 and a molecular mass of 39,554.2Da. It presents similarities to Platanaceae, Poaceae, and Cupressaceae allergenic polygalacturonases. cDNA-encoding sequence was subcloned into the pET41b vector and produced in bacteria as a His-tag fusion recombinant protein. The far-UV CD spectrum determined that rSal k 6 was folded. Immunostaining of the S. kali pollen protein extract with a rSal k 6-specific pAb and LC-MS/MS proteomic analyses confirmed the co-existence of the 28kDa band together with an allergenic band of about 47kDa in the pollen extract. Therefore, the 28kDa was assigned as a natural degradation product of the 47kDa integral polygalacturonase. The IgE-binding inhibition to S. kali pollen extract using rSal k 6 as inhibitor showed that signals directed to both protein bands of 28 and 47kDa were completely abrogated. The average prevalence of rSal k 6 among the three populations analyzed was 30%, with values correlating well with the levels of grains/m3 of Amaranthaceae pollen. Sal k 6 shares IgE epitopes with Oleaceae members (Fraxinus excelsior, Olea europaea and Syringa vulgaris), with IgE-inhibition values ranging from 20% to 60%, respectively. No IgE-inhibition was observed with plant-derived food extracts.


Assuntos
Antígenos de Plantas/metabolismo , Glicosídeos/metabolismo , Imunoglobulina E/metabolismo , Proteínas de Plantas/metabolismo , Pólen/metabolismo , Salsola/metabolismo , Amaranthaceae/química , Amaranthaceae/metabolismo , Sequência de Aminoácidos , Antígenos de Plantas/química , Sequência de Bases , Clonagem Molecular/métodos , Reações Cruzadas/fisiologia , Glicosídeos/química , Oleaceae/química , Oleaceae/metabolismo , Proteínas de Plantas/química , Pólen/química , Ligação Proteica/fisiologia , Proteômica/métodos , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/metabolismo , Salsola/química , Alinhamento de Sequência
9.
Int Arch Allergy Immunol ; 173(1): 12-22, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28486236

RESUMO

BACKGROUND: Allergic sensitization might be influenced by the lipids present in allergens, which can be recognized by natural killer T (NKT) cells on antigen-presenting cells (APCs). The aim of this study was to analyze the effect of olive pollen lipids in human APCs, including monocytes as well as monocyte-derived macrophages (Mϕ) and dendritic cells (DCs). METHODS: Lipids were extracted from olive (Olea europaea) pollen grains. Invariant (i)NKT cells, monocytes, Mϕ, and DCs were obtained from buffy coats of healthy blood donors, and their cell phenotype was determined by flow cytometry. iNKT cytotoxicity was measured using a lactate dehydrogenase assay. Gene expression of CD1A and CD1D was performed by RT-PCR, and the production of IL-6, IL-10, IL-12, and TNF-α cytokines by monocytes, Mϕ, and DCs was measured by ELISA. RESULTS: Our results showed that monocytes and monocyte-derived Mϕ treated with olive pollen lipids strongly activate iNKT cells. We observed several phenotypic modifications in the APCs upon exposure to pollen-derived lipids. Both Mϕ and monocytes treated with olive pollen lipids showed an increase in CD1D gene expression, whereas upregulation of cell surface CD1d protein occurred only in Mϕ. Furthermore, DCs differentiated in the presence of human serum enhance their surface CD1d expression when exposed to olive pollen lipids. Finally, olive pollen lipids were able to stimulate the production of IL-6 but downregulated the production of lipopolysaccharide- induced IL-10 by Mϕ. CONCLUSIONS: Olive pollen lipids alter the phenotype of monocytes, Mϕ, and DCs, resulting in the activation of NKT cells, which have the potential to influence allergic immune responses.


Assuntos
Alérgenos/imunologia , Células Apresentadoras de Antígenos/imunologia , Lipídeos/imunologia , Células T Matadoras Naturais/imunologia , Olea/imunologia , Pólen/imunologia , Antígenos CD1d/imunologia , Citocinas/imunologia , Humanos
10.
J Immunol ; 195(2): 445-9, 2015 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-26041541

RESUMO

Olive (Olea europaea) pollen constitutes one of the most important allergen sources in the Mediterranean countries and some areas of the United States, South Africa, and Australia. Recently, we provided evidence that olive pollen releases nanovesicles of respirable size, named generically pollensomes, during in vitro germination. Olive pollensomes contain allergens, such as Ole e 1, Ole e 11, and Ole e 12, suggesting a possible role in allergy. The aim of this study was to assess the contribution of pollensomes to the allergic reaction. We show that pollensomes exhibit allergenic activity in terms of patients' IgE-binding capacity, human basophil activation, and positive skin reaction in sensitized patients. Furthermore, allergen-containing pollensomes have been isolated from three clinically relevant nonphylogenetically related species: birch (Betula verrucosa), pine (Pinus sylvestris), and ryegrass (Lolium perenne). Most interesting, pollensomes were isolated from aerobiological samples collected with an eight-stage cascade impactor collector, indicating that pollensomes secretion is a naturally occurring phenomenon. Our findings indicate that pollensomes may represent widespread vehicles for pollen allergens, with potential implications in the allergic reaction.


Assuntos
Basófilos/imunologia , Hipersensibilidade/imunologia , Pólen/imunologia , Animais , Antígenos de Plantas/isolamento & purificação , Antígenos de Plantas/farmacologia , Teste de Degranulação de Basófilos , Basófilos/efeitos dos fármacos , Basófilos/patologia , Betula/química , Betula/imunologia , Estudos de Casos e Controles , Germinação , Humanos , Hipersensibilidade/sangue , Hipersensibilidade/patologia , Soros Imunes/química , Imunoglobulina E/sangue , Lolium/química , Lolium/imunologia , Camundongos , Olea/química , Olea/imunologia , Pinus/química , Pinus/imunologia , Extratos Vegetais/química , Extratos Vegetais/imunologia , Proteínas de Plantas/isolamento & purificação , Proteínas de Plantas/farmacologia , Pólen/química , Cultura Primária de Células
11.
Int J Mol Sci ; 18(8)2017 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-28812992

RESUMO

Knowledge of the susceptibility of proteins to endolysosomal proteases provides valuable information on immunogenicity. Though Ole e 1-like proteins are considered relevant allergens, little is known about their immunogenic properties and T cell epitopes. Thus, six representative molecules, i.e., Ole e 1, Fra e 1, Sal k 5, Che a 1, Phl p 11 and Pla l 1, were investigated. Endolysosomal degradation and peptide generation were simulated using microsomal fractions of JAWS II dendritic cells. Kinetics and peptide patterns were evaluated by gel electrophoresis and mass spectrometry. In silico MHC (major histocompatibility complex) class II binding prediction was performed with ProPred. Cleavage sites were assigned to the primary and secondary structure, and in silico docking experiments between the protease cathepsin S and Ole e 1 were performed. Different kinetics during endolysosomal degradation were observed while similar peptide profiles especially at the C-termini were detected. Typically, the identified peptide clusters comprised the previously-reported T cell epitopes of Ole e 1, consistent with an in silico analysis of the T cell epitopes. The results emphasize the importance of the fold on allergen processing, as also reflected by conserved cleavage sites located within the large flexible loop. In silico docking and mass spectrometry results suggest that one of the first Ole e 1 cleavages might occur at positions 107-108. Our results provided kinetic and structural information on endolysosomal processing of Ole e 1-like proteins.


Assuntos
Antígenos de Plantas , Células Dendríticas/imunologia , Epitopos de Linfócito T , Lisossomos/imunologia , Peptídeos , Proteínas de Plantas , Proteólise , Animais , Antígenos de Plantas/química , Antígenos de Plantas/imunologia , Linhagem Celular , Epitopos de Linfócito T/química , Epitopos de Linfócito T/imunologia , Camundongos , Peptídeos/química , Peptídeos/imunologia , Proteínas de Plantas/química , Proteínas de Plantas/imunologia
12.
Biochim Biophys Acta ; 1854(12): 1871-1880, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26391288

RESUMO

Proteins performing important biochemical activities in the olive tree (Olea europaea) pollen have been identified as allergens. One novel 37-kDa protein seems to be associated to the IgE-binding profile of a group of patients suffering allergy to peach and olive pollen. Three previously described olive pollen allergens exhibit very similar molecular mass. Our objective was to identify this allergen by using immunoproteomic approaches. After 2D-electrophoresis and mass spectrometry, peptide sequences from several IgE-binding spots, allowed identifying this new allergen, as well as cloning and DNA sequencing of the corresponding gene. The allergen, named Ole e 12, is a polymorphic isoflavone reductase-like protein of 308 amino acids showing 80% and 74% identity with birch and pear allergens, Bet v 6 and Pyr c 5, respectively. A prevalence of 33% in the selected population is in contrast to 4%-10% in groups of subjects suffering from pollinosis. Recombinant allergen was produced in Escherichia coli, and deeply characterised. Immunoblotting and ELISA detection as well as inhibition experiments were performed with polyclonal antisera and allergic patients' sera. The recombinant allergen retains the IgE reactivity of its natural counterpart. Close structural and immunological relationships between members of this protein family were supported by their IgG recognition in vegetable species. In summary, Ole e 12 is a minor olive pollen allergen, which gains relevance in patients allergic to peach with olive pollinosis. Proteomic approaches used to analyse this allergen provide useful tools to identify hidden allergens, relevant for several allergic populations and thus complete allergenic panels.


Assuntos
Alérgenos/química , Olea/química , Pólen/química , Proteômica , Alérgenos/imunologia , Sequência de Aminoácidos , Humanos , Imunoglobulina E/imunologia , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos
13.
Anal Chem ; 88(24): 12339-12345, 2016 12 20.
Artigo em Inglês | MEDLINE | ID: mdl-28193070

RESUMO

Autoantibodies raised against tumor-associated antigens have shown high promise as clinical biomarkers for reliable diagnosis, prognosis, and therapy monitoring of cancer. An electrochemical disposable biosensor for the specific and sensitive determination of p53-specific autoantibodies has been developed for the first time in this work. This biosensor involves the use of magnetic microcarriers (MBs) modified with covalently immobilized HaloTag fusion p53 protein as solid supports for the selective capture of specific autoantibodies. After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric signal using the system hydroquinone/H2O2 was related to the levels of p53-autoantibodies in the sample. The biosensor was applied for the analysis of sera from 24 patients with high-risk of developing colorectal cancer and 6 from patients already diagnosed with colorectal (4) and ovarian (2) cancer. The developed biosensor was able to determine p53 autoantibodies with a sensitivity higher than that of a commercial standard ELISA using a just-in-time produced protein in a simpler protocol with less sample volume and easily miniaturized and cost-effective instrumentation.


Assuntos
Autoanticorpos/sangue , Autoanticorpos/imunologia , Técnicas Biossensoriais/métodos , Neoplasias/sangue , Neoplasias/imunologia , Proteína Supressora de Tumor p53/imunologia , Técnicas Biossensoriais/instrumentação , Neoplasias Colorretais/sangue , Neoplasias Colorretais/imunologia , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Desenho de Equipamento , Feminino , Humanos , Proteínas Imobilizadas/química , Proteínas Imobilizadas/imunologia , Imunidade Humoral , Limite de Detecção , Biópsia Líquida/instrumentação , Biópsia Líquida/métodos , Modelos Moleculares , Neoplasias Ovarianas/sangue , Neoplasias Ovarianas/imunologia , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/imunologia , Proteína Supressora de Tumor p53/química
15.
Langmuir ; 32(42): 11055-11062, 2016 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-27723354

RESUMO

Aeroallergens are airborne substances-mainly proteins-capable of triggering Th2-immune responses in respiratory allergies. They enter into the body through the upper airways, reaching the mucosa afterward. Mucosae lining at the luminal side consists of an epithelial barrier completely covered by mucus and pulmonary surfactant. Both pulmonary surfactant and plasma membrane of the epithelial cells represent two physiological phospholipid-based barriers where allergens first impact before triggering their biological effects. The interaction of allergens with lipids at relevant physiological surfaces could promote structural changes on the molecule, resulting on a potential modification of its allergenic properties. In this work, we have first described the surface and phospholipid interaction capabilities of the clinically relevant aeroallergen Ole e 1, the main allergen of olive tree pollen. By using epifluorescence microscopy of Langmuir transferred films, we observed that lipid-packed ordered domains may function as a preferential location for allergen to accumulate at the air-liquid interface, an effect that is abolished in the presence of cholestenone. The possible implications of phospholipid-interfacial effects in the modification of allergen structural and functional properties will be discussed.

16.
Pediatr Allergy Immunol ; 27(7): 716-720, 2016 11.
Artigo em Inglês | MEDLINE | ID: mdl-27341427

RESUMO

BACKGROUND: Ingestion of food allergens present in maternal milk during breastfeeding has been hypothesized as a gateway to sensitization to food; however, this process could develop during pregnancy, as the maternal-fetal interface develops a Th2- and Treg-mediated environment to protect the fetus. We hypothesized that in these surroundings, unborn children are exposed to food allergens contained in the mother's diet, possibly giving rise to first sensitization. METHODS: The presence of allergens in utero was studied by analyzing amniotic fluid (AF) samples in two different stages of pregnancy: at 15-20 weeks and after delivery at term. An antibody microarray was developed to test for the most common food allergens. The array detects the presence of ten allergens from milk, fruit, egg, fish, nuts, and wheat. RESULTS: AF from 20 pregnant women was collected: eight after delivery at term and 12 from women who underwent diagnostic amniocentesis between weeks 15 and 20 of gestation. The presence of allergens was detected in all samples. Samples from amniocentesis had a higher allergen concentration than samples after delivery at term. CONCLUSIONS: We demonstrated the presence of intact major food allergens in AF samples. This early contact could explain subsequent sensitization to foods never eaten before.


Assuntos
Alérgenos/metabolismo , Líquido Amniótico/metabolismo , Hipersensibilidade Alimentar/imunologia , Proteínas do Leite/metabolismo , Efeitos Tardios da Exposição Pré-Natal/imunologia , Alérgenos/imunologia , Líquido Amniótico/imunologia , Animais , Bovinos , Dieta , Feminino , Alimentos , Humanos , Imunidade Materno-Adquirida , Imunização , Proteínas do Leite/imunologia , Gravidez , Análise Serial de Proteínas
18.
Arch Biochem Biophys ; 580: 93-101, 2015 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-26151774

RESUMO

Ole e 9 and Fra e 9 are two allergenic ß-1,3-glucanases from olive and ash tree pollens, respectively. Both proteins present a modular structure with a catalytic N-terminal domain and a carbohydrate-binding module (CBM) at the C-terminus. Despite their significant sequence resemblance, they differ in some functional properties, such as their catalytic activity and the carbohydrate-binding ability. Here, we have studied the different capability of the recombinant C-terminal domain of both allergens to bind laminarin by NMR titrations, binding assays and ultracentrifugation. We show that rCtD-Ole e 9 has a higher affinity for laminarin than rCtD-Fra e 9. The complexes have different exchange regimes on the NMR time scale in agreement with the different affinity for laminarin observed in the biochemical experiments. Utilising NMR chemical shift perturbation data, we show that only one side of the protein surface is affected by the interaction and that the binding site is located in the inter-helical region between α1 and α2, which is buttressed by aromatic side chains. The binding surface is larger in rCtD-Ole e 9 which may account for its higher affinity for laminarin relative to rCtD-Fra e 9.


Assuntos
Alérgenos/química , Antígenos de Plantas/química , Glucana 1,3-beta-Glucosidase/química , Glucanos/química , Proteínas de Plantas/química , beta-Glucosidase/química , Alérgenos/genética , Alérgenos/imunologia , Sequência de Aminoácidos , Antígenos de Plantas/genética , Antígenos de Plantas/imunologia , Sítios de Ligação , Fraxinus/química , Fraxinus/enzimologia , Expressão Gênica , Glucana 1,3-beta-Glucosidase/genética , Glucana 1,3-beta-Glucosidase/imunologia , Humanos , Modelos Moleculares , Dados de Sequência Molecular , Ressonância Magnética Nuclear Biomolecular , Olea/química , Olea/enzimologia , Pichia/genética , Pichia/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia , Pólen/química , Pólen/imunologia , Ligação Proteica , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Homologia de Sequência de Aminoácidos , beta-Glucosidase/genética , beta-Glucosidase/imunologia
19.
Int Arch Allergy Immunol ; 167(2): 83-93, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26202069

RESUMO

BACKGROUND: The incidence of Amaranthaceae pollen allergy has increased due to the desertification occurring in many countries. In some regions of Spain, Salsola kali is the main cause of pollinosis, at almost the same level as olive and grass pollen. Sal k 1 - the sensitization marker of S. kali pollinosis - is used in clinical diagnosis, but is purified at a low yield from pollen. We aimed to produce a recombinant (r)Sal k 1 able to span the structural and immunological properties of the natural isoforms from pollen, and validate its potential use for diagnosis. METHODS: Specific cDNA was amplified by PCR, cloned into the pET41b vector and used to transform BL21 (DE3) Escherichia coli cells. Immunoblotting, ELISA, basophil activation and skin-prick tests were used to validate the recombinant protein against Sal k 1 isolated from pollen. Sera and blood cells from S. kali pollen-sensitized patients and specific monoclonal and polyclonal antisera were used. RESULTS: rSal k 1 was produced in bacteria with a yield of 7.5 mg/l of cell culture. The protein was purified to homogeneity and structural and immunologically validated against the natural form. rSal k 1 exhibited a higher IgE cross-reactivity with plant-derived food extracts such as peanut, almond or tomato than with pollen sources such as Platanus acerifolia and Oleaceae members. CONCLUSIONS: rSal k 1 expressed in bacteria retains intact structural and immunological properties in comparison to the pollen-derived allergen. It spans the immunological properties of most of the isoforms found in pollen, and it might substitute natural Sal k 1 in clinical diagnosis.


Assuntos
Alérgenos , Antígenos de Plantas , Pólen/imunologia , Rinite Alérgica Sazonal/diagnóstico , Rinite Alérgica Sazonal/imunologia , Salsola/imunologia , Alérgenos/genética , Alérgenos/isolamento & purificação , Antígenos de Plantas/genética , Antígenos de Plantas/isolamento & purificação , Teste de Degranulação de Basófilos , Clonagem Molecular , Reações Cruzadas , Escherichia coli/genética , Humanos , Imunoglobulina E/metabolismo , Pólen/genética , Isoformas de Proteínas/genética , Isoformas de Proteínas/imunologia , Isoformas de Proteínas/isolamento & purificação , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Salsola/genética , Espanha
20.
Curr Allergy Asthma Rep ; 15(2): 496, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25504260

RESUMO

Over the past few decades, significant scientific progress has influenced clinical allergy practice. The biological standardization of extracts was followed by the massive identification and characterization of new allergens and their progressive use as diagnostic tools including allergen micro arrays that facilitate the simultaneous testing of more than 100 allergen components. Specific diagnosis is the basis of allergy practice and is always aiming to select the best therapeutic or avoidance intervention. As a consequence, redundant or irrelevant information might be adding unnecessary cost and complexity to daily clinical practice. A rational use of the different diagnostic alternatives would allow a significant improvement in the diagnosis and treatment of allergic patients, especially for those residing in complex pollen exposure areas.


Assuntos
Alérgenos/imunologia , Hipersensibilidade/imunologia , Pólen/imunologia , Humanos , Hipersensibilidade/diagnóstico , Metabolismo dos Lipídeos
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