P2 of Rice grassy stunt virus (RGSV) and p6 and p9 of Rice ragged stunt virus (RRSV) isolates from Vietnam exert suppressor activity on the RNA silencing pathway.

In Vietnam, the two main viruses that cause disease in rice are the Rice grassy stunt virus (RGSV) and the Rice ragged stunt virus (RRSV). Outbreaks of these two viruses have dramatically decreased rice production in Vietnam. Because natural resistance genes are unknown, an RNAi strategy may be an alternative method to develop resistance to RGSV and RRSV. However, this strategy will be efficient only if putative silencing suppressors encoded by the two viruses are neutralized. To identify these suppressors, we used the classical green fluorescent protein (GFP) agroinfiltration method in Nicotiana benthamiana. Then, we investigated the effects of viral candidate proteins on GFP expression and GFP siRNA accumulation and their interference with the short- or long-range signal of silencing. RGSV genes s2gp1, s5gp2, and s6gp1 and RRSV genes s5gp1, s6gp1, s9gp1, and s10gp1 were selected for viral silencing suppressor investigation according to their small molecular weight, the presence of cysteines, or the presence of a GW motif in related protein products. We confirmed that protein p6 of RRSV displays mild silencing suppressor activity and affects long-range silencing by delaying the systemic silencing signal. In addition, we identified two new silencing suppressors that displayed mild activity: p2 of RGSV and p9 of RRSV.

Sustainable value added material use of occurring by-products from sugar and rice production in Vietnam.

Due to the worldwide growing population, the demand on food increases, which results in the need for a higher productivity in agriculture. Generally, this leads to larger amounts of agricultural residues and by-products, which may cause severe environmental risks due to emissions from simple burning or storing, especially in emerging and developing countries. In Vietnam agricultural by-products arise in total of 118 Mt per year, whereas 80% are coming from rice and sugar cane processing. By the selection of favorable plant varieties, seed, and/or seedlings, it is possible to improve the rice and sugar yield. Besides, the by-products offer a great potential for a value-added material use. We developed a flexible portable, integrated process scheme aside from high-tech biotechnology applications. Erosion control blankets, soil improvers/composts for an improved crop yield and soil management, and adsorbents with the focus on environmental issues for cleaning of fluid streams were produced from different fractions of the residue fractions via thermo-mechanical processes. As a consequence, fossil raw material input streams, e.g. polymer based textiles, inorganic fertilizing agents, and peat/coal can be avoided. In laboratory and field tests we demonstrate the producibility and the applicability and summarize the positive impact of the aforementioned products made from rice straw and bagasse: The improved varieties as well as the addition of selected soil improvers/composts made from the by-products improve the test plants' yield and quality. The application of erosion control blankets prevents soil loss and dehydration by covering soil surface for a period of transition. The produced shaped activated carbons show mechanical and adsorption specific properties, which are comparable to commercial products.