N'-(2-Chloro-benzyl-idene)-4-methyl-benzohydrazide.

In the title compound, C(15)H(13)ClN(2)O, the mol-ecule displays a trans conformation with respect to the C=N bond. The two aromatic rings form a dihedral angle of 12.0 (3)°. In the crystal, mol-ecules are connected via N-H⋯O hydrogen bonds into chains propagating along the c-axis direction.

Randomized research on the mechanism of local oxygen therapy promoting wound healing of diabetic foot based on RNA-seq technology.

BACKGROUND: High purity oxygen therapy has good clinical efficacy in the treatment of diabetic foot (DF), but its mechanism of promoting wound healing has been unclear. METHODS: Patients with DF were randomly divided into an experimental group and a control group. The experimental group was given local oxygen therapy (LOT) by a micro-oxygen therapy instrument, which administered uninterrupted >95% pure oxygen for 24 h at a flow rate of 3 mL/h. Six skin samples from the experimental group before and after treatment underwent RNA sequencing (RNA-seq), and the differentially expressed genes (DEGs) were screened. RESULTS: The clinical results showed that the mean wound healing time of the experimental group was 26 days (P<0.05); the healing area of the experimental group was 3.1-15.3 cm3 , with a mean of 8.8 cm3 , and that of the control group was 2.4-10.4 cm3 (P<0.05). LOT promoted the healing of DF wounds mainly through the tumor necrosis factor (TNF) signaling pathway and the apoptosis pathway. CONCLUSIONS: According to our results, LOT can promote DF healing mainly by inhibiting the local oxidative stress reaction of wound skin and by inhibiting the inflammatory and apoptotic pathways. The molecular markers and pathways screened warrant further study.

Construction, implementation and testing of an image identification system using computer vision methods for fruit flies with economic importance (Diptera: Tephritidae).

BACKGROUND: Many species of Tephritidae are damaging to fruit, which might negatively impact international fruit trade. Automatic or semi-automatic identification of fruit flies are greatly needed for diagnosing causes of damage and quarantine protocols for economically relevant insects. RESULTS: A fruit fly image identification system named AFIS1.0 has been developed using 74 species belonging to six genera, which include the majority of pests in the Tephritidae. The system combines automated image identification and manual verification, balancing operability and accuracy. AFIS1.0 integrates image analysis and expert system into a content-based image retrieval framework. In the the automatic identification module, AFIS1.0 gives candidate identification results. Afterwards users can do manual selection based on comparing unidentified images with a subset of images corresponding to the automatic identification result. The system uses Gabor surface features in automated identification and yielded an overall classification success rate of 87% to the species level by Independent Multi-part Image Automatic Identification Test. CONCLUSION: The system is useful for users with or without specific expertise on Tephritidae in the task of rapid and effective identification of fruit flies. It makes the application of computer vision technology to fruit fly recognition much closer to production level. © 2016 Society of Chemical Industry.

Could microbe stimulated maggots become a targeted natural antibiotics family?

Maggot debridement therapy plays an important role in treatment of diabetic foot ulcers and other chronic infectious wounds, cause of this is its extremely low drug resistance. However, the microbe stimulated maggot, we may call it a derivative of normal sterile maggot, could exhibit stronger bacterial or bactericidal effects. Methods of the pretreatment on maggot was different germ solution were artificially mixed and added with originally sterile maggots, the novel secretions were collected. Some of this have been demonstrated by plate test and telescope analysis. Thus, we hypothesize that maggot especially the larvae of Lucilia sericata was conducted as the germ irritant receptor, and diverse germs interacted with it, at last, novel secretions/excretions we got will offer a great help to the general surgery clinicians as well as researchers who are interested in novel antibiotics discovery.

A potential material to cut down infection caused by application of artificial muscles.

Artificial muscles are so important that can be used to cure prosthetic limbs. A new kind of taurine Schiff base sodium was synthesized by a series of chemical reactions, which may be applied to strengthen antibacterial activity of artificial muscle. The bioactivity of this material was screened by cytotoxicity test, antibacterial test, and thermal gravity test and so on. All results told us that this material had low toxicity, high antibacterial activity and thermal stability. Combine our deep studies on pharmacological activity of the active material with our knowledge on artificial muscles; we want to know if we can put this material into the content of artificial muscle, in order to strengthen its antimicrobial activity, so that the pains of the patients who were applied artificial muscle would be relieved.

Clinical research on the bio-debridement effect of maggot therapy for treatment of chronically infected lesions.

OBJECTIVE: To evaluate the bio-debridement effect of maggot therapy for treating chronically infected lesions. METHODS: A retrospective study was conducted of 25 patients with diabetic foot ulcers and 18 patients with pressure ulcers after spinal cord injury treated by maggot therapy or traditional dressing. Changes in the lesions were observed and bacterial cultures tested. RESULTS: All ulcers healed completely. The times taken to achieve bacterial negativity, granulation and healing of lesions were all significantly shorter in the maggot therapy group than in the control group, both for diabetic foot ulcers (P < 0.05) and pressure ulcers (P < 0.05). CONCLUSION: Maggot therapy is a safe and effective method for treating chronically infected lesions.

Salvage of amputated upper extremities with temporary ectopic implantation followed by replantation at a second stage.

Salvage of the complex amputation of extremities, such as combined with devastating segmental injuries, extensive soft tissue defect, and multiple important organ injuries, continues to be a challenge for plastic surgeons. Temporary ectopic implantation of the amputated part to a healthy recipient site allows the patient to recover from critical combined injuries, radical debridements, and soft tissue repair. In this article, the authors report two cases of temporary ectopic implantation of complexly amputated forearms, followed by successful replantation to their anatomic positions at a second stage. The contralateral upper extremity is an acceptable recipient site for temporary ectopic implantation. In secondary replantation, a cross-arm flap can be designed to carry the vascular pedicle from the ectopic implantation recipient to improve blood supply to the replanted part when the second blood supply is established. The authors validated that temporary ectopic implantation of amputated parts provides an alternative procedure for the salvage of amputated extremities under special circumstances.

Temporary ectopic implantation for salvage of amputated lower extremities: case reports.

Two cases of temporary ectopic implantation of a complex amputated foot, followed by replantation to its anatomic position, are reported. Both cases of amputated foot were complicated by devastating soft-tissue injuries in the proximal stump of the amputation, fracture of the femur, and hemorrhagic shock, which ruled out the possibility of primary foot replantation. Both feet were temporarily ectopically implanted onto the contralateral legs, with microvascular anastomoses of the vessels to the recipient posterior tibial artery and saphenous vein. When the patient's general condition allowed, and the soft-tissue defects were repaired, the ectopic implanted feet were replanted to their anatomic positions. Both feet survived the temporary ectopic implantation and second-stage replantation. The length of the injured legs was maintained, and the feet regained their function in 4- and 6-month follow-ups. We conclude that temporary ectopic implantation of amputated parts provides an innovative procedure for the salvage of amputated extremities under special circumstances. A contralateral healthy extremity is an ideal recipient site for temporary ectopic implantation. The temporary ectopic implantation and second-stage replantation of an amputated foot and distal leg with indications can obtain satisfactory results.

[Temporary ectopic implantation of amputated foot].

OBJECTIVE: To investigate an effective technique of temporary ectopic implantation for amputated extremity under complex condition. METHODS: Two cases of amputated foot, which could not be implanted primarily, were treated with temporary ectopic implantation. The other leg of patient was chosen as recipient site. The posterotibial artery and saphenous vein were chosen as recipient vessels. When the general condition and the proximal condition of the amputated part were suitable, the ectopic implanted feet were transferred to their anatomic positions. RESULTS: All the feet survived after the replantation. The injured limbs recovered their normal length and sensation. The patients could walk after 4-6 months. CONCLUSION: Temporary ectopic implantation is an ideal technique for the salvage of amputated limb and organ under special condition. Severed foot and lower segment of the leg under complex condition were the best indication for the temporary ectopic implantation.