Long-isoform NFE2L1 silencing inhibits acquisition of malignant phenotypes induced by arsenite in human bronchial epithelial cells.

Chronic arsenic exposure is associated with the increased risk of several types of cancer, among which, lung cancer is the most deadly one. Nuclear factor erythroid 2 like 1 (NFE2L1), a transcription factor belonging to CNC-bZIP family, regulates multiple important cellular functions in response to acute arsenite exposure. However, the role of NFE2L1 in lung cancer induced by chronic arsenite exposure is unknown. In this study, we firstly showed that chronic arsenite exposure (36 weeks) led to epithelial-mesenchymal transition (EMT) and malignant transformation in human bronchial epithelial cells (BEAS-2B). During the process of malignant transformation, the expression of long isoforms of NFE2L1 (NFE2L1-L) was elevated. Thereafter, BEAS-2B cells with NFE2L1-L stable knockdown (NFE2L1-L-KD) was chronically exposed to arsenite. As expected, silencing of NFE2L1-L gene strikingly inhibited the arsenite-induced EMT and the subsequent malignant transformation. Additionally, NFE2L1-L silencing suppressed the transcription of EMT-inducer SNAIL1 and increased the expression of E-cadherin. Conversely, NFE2L1-L overexpression increased SNAIL1 transcription but decreased E-cadherin expression. Collectively, our data suggest that NFE2L1-L promotes EMT by positively regulating SNAIL1 transcription, and is involved in malignant transformation induced by arsenite.

Pulsed vapor cell atomic clock with a differential Faraday rotation angle detection.

Laser intensity noise is one of the main limiting factors in pulsed vapor cell clocks. To reduce the contribution of the laser intensity noise to detection signal in the pulsed optically pumped atomic clock, a scheme based on the differential Faraday rotation angle is proposed. Theoretically, the Ramsey fringes, the sensitivity of clock frequency to laser intensity fluctuation and the signal to noise ratio for absorption, differential, and Faraday rotation angle methods are calculated and compared. Using a Wollaston prism rotated 45°relative to the incident polarization, and two photodetectors, Ramsey fringes of three detection methods are obtained simultaneously. In the proposed scheme, the long-term Faraday rotation angle fluctuation is 0.66% at 30000s, which is much smaller than fluctuation of traditional absorption signal 3.9% at 30000s. And the contribution of laser intensity noise to clock instability is also reduced. Using optimized photodetector with high common mode rejection ratio, a better performance should be expected. This proposed scheme is attractive for the development of high performance vapor clock based on pulsed optically pumped.

A Compact Laser System for the Pulsed Optically Pumped Rubidium Cell Atomic Clock.

We describe the design and implementation of a compact laser system for the pulsed optically pumped (POP) rubidium (Rb) cell atomic clock. The laser system includes packaged optics for sub-Doppler absorption, acousto-optic modulation and light beam expansion, and dedicated electronics for laser diode reliable single-mode operation and laser frequency stabilization. With beat measurements between two identical laser systems, the laser frequency stability was found to be 3.0×10-12 for averaging times from 1 to 60 s and it reached 3.5×10-12 at 10 000 s averaging time. Based on the compact laser system, the short-term stability of the Rb cell atomic clock in pulsed regime was approximately [Formula: see text], which is in reasonable agreement with the estimated [Formula: see text]. The compact laser system is significant in terms of the development of transportable and high-performance Rb atomic clock prototypes.