Preparation of lignin nanoparticles via ultra-fast microwave-assisted fractionation of lignocellulose using ternary deep eutectic solvents.

Lignin separation from natural lignocellulose for the preparation of lignin nanoparticles (LNPs) is often challenging owing to the recalcitrant and complex structure of lignocellulose. This paper reports a strategy for the rapid synthesis of LNPs via microwave-assisted lignocellulose fractionation using ternary deep eutectic solvents (DESs). A novel ternary DES with strong hydrogen bonding was prepared using choline chloride, oxalic acid, and lactic acid in a 1:0.5:1 ratio. Efficient fractionation of rice straw (0.5 × 2.0 cm) (RS) was realized by the ternary DES under microwave irradiation (680 W) within only 4 min, and 63.4% of lignin could be separated from the RS to prepare LNPs with a high lignin purity (86.8%), an average particle size of 48-95 nm, and a narrow size distribution. The mechanism of lignin conversion was also investigated, which revealed that dissolved lignin aggregated into LNPs via π-π stacking interactions.

The risk factors for injuries in parachuting and load exposure in the training of Chinese paratroopers.

BACKGROUND: Parachutists are generally recognized as a "high-risk" group among military personnel. However, the findings came mostly from data analysis without soldiers as subjects. OBJECTIVE: This study aimed to investigate the injury prevalence in Chinese paratroopers on-site and determine the relationship between injury and risk factors encountered during parachuting and land-based training. METHODS: This study consisted of a field study with questionnaire and an experiment on muscle load during the simulated training exercise of platform jumping with surface electromyography (EMG), in which 7230 paratroopers and 38 soldiers were involved respectively. Chi-square test was used for the injury rate analysis, ANOVA and t-test for comparison of EMG data, and logistic regression for the analysis of multiple factors. Taking both intensity and time into consideration, jump-years (J-yrs) was used as a complex indicator for exposure to parachuting. Either injury per 1000 jumps or injured persons per 100 soldiers were calculated as injury prevalence. RESULTS: The overall injury rate among Chinese parachutists was found to be 13.9 injuries per 1000 parachute jumps and 24.5% based on personnel. The person-based injury rate increased with the exposure level significantly (χ2 = 142.06, 2-sided, P < 0.05; trend test also significantly). Among the identified risk factors the uneven terrain was ranked as the most important one by logistic analysis. The EMG amplitude in MVE% increased with the platform height of all the 8 measured muscles and even reached 100% in 4 muscles, showing a high impact at landing. In addition, some characteristics of parachuting injury were also revealed by the injury type and site analysis. CONCLUSION: A dose-response relationship between parachuting and injury was observed significantly in the survey of Chinese paratroopers. Their injury rate was found to be relatively higher than the reported internationally. Landing impact as a critical point for injury seemed to be proved by the investigation and also the experiment with EMG measurement. It is suggested for future studies, to use the person-based injury rate, landing studied in work physiology and with consideration of different landing skills.

Deep learning based radiomics for gastrointestinal cancer diagnosis and treatment: A minireview.

Gastrointestinal (GI) cancers are the major cause of cancer-related mortality globally. Medical imaging is an important auxiliary means for the diagnosis, assessment and prognostic prediction of GI cancers. Radiomics is an emerging and effective technology to decipher the encoded information within medical images, and traditional machine learning is the most commonly used tool. Recent advances in deep learning technology have further promoted the development of radiomics. In the field of GI cancer, although there are several surveys on radiomics, there is no specific review on the application of deep-learning-based radiomics (DLR). In this review, a search was conducted on Web of Science, PubMed, and Google Scholar with an emphasis on the application of DLR for GI cancers, including esophageal, gastric, liver, pancreatic, and colorectal cancers. Besides, the challenges and recommendations based on the findings of the review are comprehensively analyzed to advance DLR.

[Aspirin-PEI-beta-CyD as a novel non-viral vector for gene transfer].

OBJECTIVE: To develop a novel non-viral gene delivery vector based on PEI-beta-CyD as backbone modified with aspirin, and to identify its physicochemical characters. METHODS: 1, 1-carbonyldiimidazole (CDI) was used to bind aspirin onto PEI-beta-CyD to form PEI-beta-CyD-ASP. (1)H-NMR, FT-IR, UV and XRD were used to confirm the polymer structure. The ability of condensation was demonstrated by gel retardation assay. MTT assay was used to test the cell viability in B16, Hela and A293 cell lines. Transfection efficiency of the polymer was tested in B16 cells. RESULT: The structure of PEI-beta-CyD-ASP was confirmed by (1)H-NMR, FT-IR, UV and XRD, which efficiently condensed plasmid DNA at the N/P ratio of 4. The copolymer showed low cytotoxicity and high transfection efficiency in B16 cells. CONCLUSION: The synthesized aspirin-PEI-beta-CyD might be a potential gene delivery vector.

[Poly-aspartamide-glutamic acid grafted low molecular weight polyethylenimine as a novel non-viral gene vector].

OBJECTIVE: To develop a novel gene delivery vector with poly-aspartamide-glutamic acid and polyethylenimine as the backbone. METHODS: alpha, beta-poly-(N-2-hydroxypropyl)-D, L-aspartamide-glutamic acid (PHPAG) was synthesized and low molecular weight polyethylenimine (PEI 1.8 kDa) was grafted to form PHPAG-PEI 1800. Chemical and biological characterization of the polymer was identified. RESULT: The polymer was confirmed by (1)H-NMR, and the molecular weight was about 1.2 x 10(4). The ability of DNA binding was showed by gel retardation assay at N/P ratio of 3. 5. MTT assay showed that the polymer was non toxic in COS-7 and A293 cell lines. In vitro test demonstrated that it had high transfection efficiency in B16 and Hela cell lines. CONCLUSION: PHPAG-PEI 1800 was successfully synthesized,which might be a potential vector for gene delivery.

Polyhydroxyalkanoate-Modified Bacterium Regulates Biomass Structure and Promotes Synthesis of Carbon Materials for High-Performance Supercapacitors.

Biomass-derived carbons have been extensively explored as electrode materials in supercapacitors. However, the type of biomass selected and its specific structure affects the synthesis of the advanced biomass-derived carbon materials. A green and facile method for the synthesis of carbon material with nanoscale and microscale porous structures for supercapacitors has been developed, based on regulating the original cell structure of the bacterial strain. The cell structure is modified in situ by regulating the accumulation of polyhydroxyalkanoate under controlled cultivation conditions. The novel bacterial in situ modification and nitrogen doping endow this hierarchically derived carbon material with improved performance. This material exhibits an extremely high specific capacitance (420 F g-1 at 1 A g-1 ) and long cycling stability (97 % capacitance retention after 10 000 cycles at 5 A g-1 ) in aqueous electrolytes. More importantly, the symmetric supercapacitor delivers a superior energy density of 60.76 Wh kg-1 at 625 W kg-1 in an ionic liquid electrolyte system. Moreover, all components in the synthesis are low in cost, environmentally friendly, and biocompatible. With these unique features, the bacterial self-modification mode opens new avenues into the design and production of a wide range of hierarchical structures.

Response of Cupriavidus basilensis B-8 to CuO nanoparticles enhances Cr(VI) reduction.

CuO nanoparticles (NPs) released into aqueous environments induce metal toxicity, which generally exerts negative effects on various organisms and leads to great challenge for wastewater biotreatment. In this study, a promotion effect of CuO NPs on biological process was first found. Cr(VI) reduction by Cupriavidus basilensis B-8 (hereafter B-8) was enhanced in the presence of CuO NPs. The efficiency of Cr(VI) bioreduction was much higher with B-8 and CuO NPs (approximately 100%) than with B-8 (approximately 37.6%) and CuO NPs (39.9-44.7%) alone, indicating a stimulatory effect of CuO NPs on Cr(VI) reduction by B-8. Our material analyses revealed different response mechanisms of B-8 to Cr(VI), with and without CuO NPs. The addition of CuO NPs influenced the interaction of Cr(VI) with the N-, P-, S-, and C-related functional groups of B-8. Transcriptomic analysis indicated that multiple mechanisms, including Cr(VI) uptake and reactive oxygen species detoxification, were induced by Cr(VI). Many genes involved in various metabolic processes were significantly upregulated by the addition of CuO NPs. To a certain extent, the pressure of DNA repairment by B-8 induced by Cr(VI) was also alleviated by the presence of CuO NPs. They contributed to facilitate B-8 growth and enhance Cr(VI) reduction, even with 50 mg/L Cr(VI). This study not only elaborated the mechanisms of bacterial Cr(VI) reduction when enhanced by CuO NPs, but also provided a novel perspective for wastewater biotreatment.

Bacteria-enhanced dilute acid pretreatment of lignocellulosic biomass.

Pretreatment is indispensable for the large-scale and low-cost bio-products production from lignocellulosic biomass. Herein, a new bacteria-enhanced dilute acid pretreatment (BE-DAP) strategy was introduced. Cupriavidus basilensis B-8 as a potential bacterium for lignin degradation was employed. Multi-scale characterizations on the physicochemical structure of rice straw indicated that Cupriavidus basilensis B-8 could act on the lignin droplets formed in dilute acid pretreatment (DAP), and dig out these droplets to recover cracks and holes on rice straw surface, leaving an opened and porous structure for the easy access of enzyme to inner cellulose. Eventually, the enzymatic digestibility of RS was increased by 35-70% and 173-244% in BE-DAP compared to DAP pretreated and untreated RS, respectively. The BE-DAP strategy, as well as its physicochemical mechanism, opened new perspectives for lignocellulose pretreatment.

[Genetic diversity and differentiation of Fagopyrum tataricum and its related species in China].

Genetic diversity and differentiation among 50 populations of total 8 species and 1 variety of Fagopyrum tataricum and its related species from 27 counties of Sichuan and Yunnan provinces, Southwest China were investigated using allozyme electrophoresis. Seven enzymesat 12 loci were assessed. The result shows that among 8 species and 1 variety, inbreeding coefficient is -0.54(-)-0.80, which means hterozogote excess. Higher genetic diversity was found in the wild related species of F. tataricum than in F. tataricum. F. dibotrys has the highest Fst value, which means that more genetic variations exist among its populations. The lowest Fst value shows that less genetic variations exist among population of F. gracilipes whic is close to panmictic There is almost no genetic differentiation between populations of F. statice. Genetic similarity is 55.3%-65.2% between species of F. tataricum and its related species, which is close to the average level among species in seed plants. According to the cluster analysis of genetic similarity, F. gracillipes is most close to F. tataricum and F. esculentum. Jinsha River region is the distributional and original center of F. tataricum and its related species.

Synthesis and structure-activity relationships of azamacrocyclic C-X-C chemokine receptor 4 antagonists: analogues containing a single azamacrocyclic ring are potent inhibitors of T-cell tropic (X4) HIV-1 replication.

Bis-tetraazamacrocycles such as the bicyclam AMD3100 (1) are a class of potent and selective anti-HIV-1 agents that inhibit virus replication by binding to the chemokine receptor CXCR4, the coreceptor for entry of X4 viruses. By sequential replacement and/or deletion of the amino groups within the azamacrocyclic ring systems, we have determined the minimum structural features required for potent antiviral activity in this class of compounds. All eight amino groups are not required for activity, the critical amino groups on a per ring basis are nonidentical, and the overall charge at physiological pH can be reduced without compromising potency. This approach led to the identification of several single ring azamacrocyclic analogues such as AMD3465 (3d), 36, and 40, which exhibit EC(50)'s against the cytopathic effects of HIV-1 of 9.0, 1.0, and 4.0 nM, respectively, antiviral potencies that are comparable to 1 (EC(50) against HIV-1 of 4.0 nM). More importantly, however, the key structural elements of 1 required for antiviral activity may facilitate the design of nonmacrocyclic CXCR4 antagonists suitable for HIV treatment via oral administration.

Allozyme variation and population genetic structure of Betula alnoides from Guangxi, China.

Allozyme variation and population genetic structure of Betula alnoides Buch Ham. ex D. Don in 11 natural populations from Guangxi Zhuang Autonomous Region, China, were investigated by starch gel electrophoresis. Variation at 15 loci from 10 enzyme systems was analyzed. Allozyme analysis revealed a high level of genetic variation in this species, with percentage of polymorphic loci (P(p)), the average number of alleles per locus (A(p)), and the expected heterozygosity (H(ep)) being 55.2%, 2.0, and 0.204, respectively, which exceeds the average level among out-crossing wind-pollinated woody species at the population level. At the species level, P(s), A(s), and H(es) were 60.0%, 2.67, and 0.206, respectively. The observed heterozygosity (H(op)) was higher than H(ep), indicating the existence of natural selection against homozygotes. The negative fixation index (F = -0.216) implied a significant excess of heterozygosity at the population level Among-population differentiation (F(ST)) accounted for 4.0% of the total variation. No significant correlation was detected between the genetic distance and geographic distance among populations. Extensive gene flow was inferred, based on the allozyme data (N(m) = 6.000 from F(ST), N(m) = 5.605 from the "private allele" method). The results demonstrated that the fragmentation status of B. alnoides had no remarkable effects on the population genetic structure of this species. Some populations are recommended for both in situ genetic conservation and germplasm collection for breeding programs.