RESUMO
Spider silk is renowned for its impressive mechanical properties. It is one of the strongest known biomaterials, possessing mechanical properties that outmatch both steel and Kevlar. However, the farming of spiders for their silk is unfeasible. Consequently, production of recombinant spider silk proteins (spidroins) in more amenable hosts is an exciting field of research. For large-scale production to be viable, a heterologous silk production system that is both highly efficient and cost effective is essential. Genes encoding recombinant spidroin have been expressed in bacterial, yeast, insect, and mammalian cells, in addition to many other platforms. This review discusses the recent advances in exploiting an increasingly diverse range of host platforms in the heterologous production of recombinant spidroins.
Assuntos
Fibroínas , Proteínas Recombinantes , Seda , Animais , Bactérias/genética , Biotecnologia/tendências , Fibroínas/genética , Insetos/genética , Mamíferos/genética , Proteínas Recombinantes/genética , Seda/genética , Leveduras/genéticaRESUMO
Their biochemical versatility and biotechnological importance make actinomycete bacteria attractive targets for ambitious genetic engineering using the toolkit of synthetic biology. But their complex biology also poses unique challenges. This mini review discusses some of the recent advances in synthetic biology approaches from an actinomycete perspective and presents examples of their application to the rational improvement of industrially relevant strains.