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Computer-assisted 4D manual cell tracking has been a valuable method for understanding spatial-temporal dynamics of embryogenesis (e.g., Stach & Anselmi BMC Biol, 13(113), 1-11 2015; Vellutini et al. BMC Biol, 15(33), 1-28 2017; Wolff et al. eLife, 7, e34410 2018) since the method was introduced in the late 1990s. Since two decades SIMI® BioCell (Schnabel et al. Dev Biol, 184, 234-265 1997), a software which initially was developed for analyzing data coming from the, at that time new technique of 4D microscopy, is in use. Many laboratories around the world use SIMI BioCell for the manual tracing of cells in embryonic development of various species to reconstruct cell genealogies with high precision. However, the software has several disadvantages: limits in handling very large data sets, the virtually no maintenance over the last 10 years (bound to older Windows versions), the difficulty to access the created cell lineage data for analyses outside SIMI BioCell, and the high cost of the program. Recently, bioinformatics, in close collaboration with biologists, developed new lineaging tools that are freely available through the open source image processing platform Fiji. Here we introduce a software tool that allows conversion of SIMI BioCell lineage data to a format that is compatible with the Fiji plugin MaMuT (Wolff et al. eLife, 7, e34410 2018). Hereby we intend to maintain the usability of SIMI BioCell created cell lineage data for the future and, for investigators who wish to do so, facilitate the transition from this software to a more convenient program.
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Invertebrados/citologia , Software , Animais , Linhagem da Célula , Desenvolvimento Embrionário , Invertebrados/classificação , Invertebrados/embriologia , Masculino , MitoseRESUMO
BACKGROUND: Over the last years, the amphipod crustacean Parhyale hawaiensis has developed into an attractive marine animal model for evolutionary developmental studies that offers several advantages over existing experimental organisms. It is easy to rear in laboratory conditions with embryos available year-round and amenable to numerous kinds of embryological and functional genetic manipulations. However, beyond these developmental and genetic analyses, research on the architecture of its nervous system is fragmentary. In order to provide a first neuroanatomical atlas of the brain, we investigated P. hawaiensis using immunohistochemical labelings combined with laser-scanning microscopy, X-ray microcomputed tomography, histological sectioning and 3D reconstructions. RESULTS: As in most amphipod crustaceans, the brain is dorsally bent out of the body axis with downward oriented lateral hemispheres of the protocerebrum. It comprises almost all prominent neuropils that are part of the suggested ground pattern of malacostracan crustaceans (except the lobula plate and projection neuron tract neuropil). Beyond a general uniformity of these neuropils, the brain of P. hawaiensis is characterized by an elaborated central complex and a modified lamina (first order visual neuropil), which displays a chambered appearance. In the light of a recent analysis on photoreceptor projections in P. hawaiensis, the observed architecture of the lamina corresponds to specialized photoreceptor terminals. Furthermore, in contrast to previous descriptions of amphipod brains, we suggest the presence of a poorly differentiated hemiellipsoid body and an inner chiasm and critically discuss these aspects. CONCLUSIONS: Despite a general uniformity of amphipod brains, there is also a certain degree of variability in architecture and size of different neuropils, reflecting various ecologies and life styles of different species. In contrast to other amphipods, the brain of P. hawaiensis does not display any striking modifications or bias towards processing one particular sensory modality. Thus, we conclude that this brain represents a common type of an amphipod brain. Considering various established protocols for analyzing and manipulating P. hawaiensis, this organism is a suitable model to gain deeper understanding of brain anatomy e.g. by using connectome approaches, and this study can serve as first solid basis for following studies.
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BACKGROUND: The duplication of genes can occur through various mechanisms and is thought to make a major contribution to the evolutionary diversification of organisms. There is increasing evidence for a large-scale duplication of genes in some chelicerate lineages including two rounds of whole genome duplication (WGD) in horseshoe crabs. To investigate this further, we sequenced and analyzed the genome of the common house spider Parasteatoda tepidariorum. RESULTS: We found pervasive duplication of both coding and non-coding genes in this spider, including two clusters of Hox genes. Analysis of synteny conservation across the P. tepidariorum genome suggests that there has been an ancient WGD in spiders. Comparison with the genomes of other chelicerates, including that of the newly sequenced bark scorpion Centruroides sculpturatus, suggests that this event occurred in the common ancestor of spiders and scorpions, and is probably independent of the WGDs in horseshoe crabs. Furthermore, characterization of the sequence and expression of the Hox paralogs in P. tepidariorum suggests that many have been subject to neo-functionalization and/or sub-functionalization since their duplication. CONCLUSIONS: Our results reveal that spiders and scorpions are likely the descendants of a polyploid ancestor that lived more than 450 MYA. Given the extensive morphological diversity and ecological adaptations found among these animals, rivaling those of vertebrates, our study of the ancient WGD event in Arachnopulmonata provides a new comparative platform to explore common and divergent evolutionary outcomes of polyploidization events across eukaryotes.
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Evolução Molecular , Duplicação Gênica , Genoma , Aranhas/genética , Animais , Feminino , Masculino , SinteniaRESUMO
Antifungal bioactivity-guided fractionation of the organic extract of the sponge Polymastia boletiformis, collected from the west coast of Ireland, led to the isolation of two new sulfated steroid-amino acid conjugates (1 and 2). Extensive 1D and 2D NMR analyses in combination with quantum mechanical calculations of the electronic circular dichroism (ECD) spectra, optical rotation, and 13C chemical shifts were used to establish the chemical structures of 1 and 2. Both compounds exhibited moderate antifungal activity against Cladosporium cucumerinum, while compound 2 was also active against Candida albicans. Marine natural products containing steroidal and amino acid constituents are extremely rare in nature.
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Antifúngicos/isolamento & purificação , Candida albicans/efeitos dos fármacos , Colestadienos/isolamento & purificação , Cladosporium/efeitos dos fármacos , Descoberta de Drogas , Glicina/análogos & derivados , Poríferos/química , Animais , Antifúngicos/química , Antifúngicos/farmacologia , Oceano Atlântico , Candida albicans/crescimento & desenvolvimento , Colestadienos/química , Colestadienos/farmacologia , Cromatografia Líquida de Alta Pressão , Dicroísmo Circular , Cladosporium/crescimento & desenvolvimento , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Glicina/química , Glicina/isolamento & purificação , Glicina/farmacologia , Irlanda , Espectroscopia de Ressonância Magnética , Metilação , Estrutura Molecular , Poríferos/crescimento & desenvolvimento , Teoria Quântica , Espectrometria de Massas por Ionização por Electrospray , Estereoisomerismo , Compostos de Enxofre/química , Compostos de Enxofre/isolamento & purificação , Compostos de Enxofre/farmacologiaRESUMO
BACKGROUND: Malacostracan evolutionary history has seen multiple transformations of ontogenetic mode. For example direct development in connection with extensive brood care and development involving planktotrophic nauplius larvae, as well as intermediate forms are found throughout this taxon. This makes the Malacostraca a promising group for study of evolutionary morphological diversification and the role of heterochrony therein. One candidate heterochronic phenomenon is represented by the concept of the 'egg-nauplius', in which the nauplius larva, considered plesiomorphic to all Crustacea, is recapitulated as an embryonic stage. RESULTS: Here we present a comparative investigation of embryonic muscle differentiation in four representatives of Malacostraca: Gonodactylaceus falcatus (Stomatopoda), Neocaridina heteropoda (Decapoda), Neomysis integer (Mysida) and Parhyale hawaiensis (Amphipoda). We describe the patterns of muscle precursors in different embryonic stages to reconstruct the sequence of muscle development, until hatching of the larva or juvenile. Comparison of the developmental sequences between species reveals extensive heterochronic and heteromorphic variation. Clear anticipation of muscle differentiation in the nauplius segments, but also early formation of longitudinal trunk musculature independently of the teloblastic proliferation zone, are found to be characteristic to stomatopods and decapods, all of which share an egg-nauplius stage. CONCLUSIONS: Our study provides a strong indication that the concept of nauplius recapitulation in Malacostraca is incomplete, because sequences of muscle tissue differentiation deviate from the chronological patterns observed in the ectoderm, on which the egg-nauplius is based. However, comparison of myogenic sequences between taxa supports the hypothesis of a zoea-like larva that was present in the last common ancestor of Eumalacostraca (Malacostraca without Leptostraca). We argue that much of the developmental sequences of larva muscle patterning were retained in the eumalacostracan lineage despite the reduction of free swimming nauplius larvae, but was severely reduced in the peracaridean clade.
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BACKGROUND: Cell lineage studies in amphipods have revealed an early restriction of blastomere fate. The mesendodermal cell lineage is specified with the third cleavage of the egg. We took advantage of this stereotyped mode of development by fluorescently labeling the mesodermal precursors in embryos of Orchestia cavimana and followed the morphogenesis of the mesodermal cell layer through embryonic development. RESULTS: The mesoderm of the trunk segments is formed by a very regular and stereotypic cell division pattern of the mesoteloblasts and their segmental daughters. The head mesoderm in contrast is generated by cell movements and divisions out of a mesendodermal cell mass. Our reconstructions reveal the presence of three different domains within the trunk mesoderm of the later embryo. We distinguish a cell group median to the limbs, a major central population from which the limb mesoderm arises and a dorsolateral branch of mesodermal cells. CONCLUSIONS: Our detailed description of mesodermal development relates different precursor cell groups to distinct muscle groups of the embryo. A dorsoventral subdivision of mesoderm is prepatterned within the longitudinal mesodermal columns of the germ-band stage. This makes amphipods excellent crustacean models for studying mesodermal differentiation on a cellular and molecular level.
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Anfípodes/embriologia , Padronização Corporal , Linhagem da Célula , Mesoderma/embriologia , Anfípodes/citologia , Animais , Movimento Celular , Extremidades/embriologia , Mesoderma/citologiaRESUMO
The cobweb spider Parasteatoda tepidariorum (C. L. Koch, 1841; syn.: Achaearanea tepidariorum) has become an important study organism in developmental biology and evolution as well as in genetics. Besides Cupiennius salei, it has become a chelicerate model organism for evo-devo studies in recent years. However, a staging system taking into account the entire development, and detailed enough to apply to modern studies, is still required. Here we describe the embryonic development of P. tepidariorum and provide a staging system which allows easy recognition of the distinct stages using simple laboratory tools. Differences between P. tepidariorum and other chelicerates, primarily C. salei, are discussed. Furthermore, cocoon production and the first postembryonic moulting procedure are described. Schematic drawings of all stages are provided to ease stage recognition.
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Aranhas/embriologia , Aranhas/ultraestrutura , Animais , Embrião não Mamífero/ultraestrutura , Desenvolvimento Embrionário , Extremidades/embriologiaRESUMO
Arthropods are the most abundant and diverse animals on earth. Among them, pancrustaceans are an ancient and morphologically diverse group, comprising a wide range of aquatic and semi-aquatic crustaceans as well as the insects, which emerged from crustacean ancestors to colonize most terrestrial habitats. Within insects, Drosophila stands out as one of the most powerful animal models, making major contributions to our understanding of development, physiology and behavior. Given these attributes, crustaceans provide a fertile ground for exploring biological diversity through comparative studies. However, beyond insects, few crustaceans are developed sufficiently as experimental models to enable such studies. The marine amphipod Parhyale hawaiensis is currently the best established crustacean system, offering year-round accessibility to developmental stages, transgenic tools, genomic resources, and established genetics and imaging approaches. The Parhyale research community is small but diverse, investigating the evolution of development, regeneration, aspects of sensory biology, chronobiology, bioprocessing and ecotoxicology.
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Anfípodes , Artrópodes , Anfípodes/genética , Animais , Artrópodes/genética , Genoma , Modelos AnimaisRESUMO
Light sheet fluorescence microscopy (LSFM) has become a method of choice for live imaging because of its fast acquisition and reduced photobleaching and phototoxicity. Despite the strengths and growing availability of LSFM systems, no generalized LSFM mounting protocol has been adapted for live imaging of post-embryonic stages of C. elegans. A major challenge has been to develop methods to limit animal movement using a mounting media that matches the refractive index of the optical system. Here, we describe a simple mounting and immobilization protocol using a refractive-index matched UV-curable hydrogel within fluorinated ethylene propylene (FEP) tubes for efficient and reliable imaging of larval and adult C. elegans stages.
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BACKGROUND: The spider Cupiennius salei (Keyserling 1877) has become an important study organism in evolutionary and developmental biology. However, the available staging system for its embryonic development is difficult to apply to modern studies, with strong bias towards the earliest developmental stages. Furthermore, important embryonic events are poorly understood. We address these problems, providing a new description of the embryonic development of C. salei. The paper also discusses various observations that will improve our understanding of spider development. RESULTS: Conspicuous developmental events were used to define numbered stages 1 to 21. Stages 1 to 9 follow the existing staging system for the spider Achaearanea tepidariorum, and stages 10 to 21 provide a high-resolution description of later development. Live-embryo imaging shows cell movements during the earliest formation of embryonic tissue in C. salei. The imaging procedure also elucidates the encircling border between the cell-dense embryo hemisphere and the hemisphere with much lower cell density (a structure termed 'equator' in earlier studies). This border results from subsurface migration of primordial mesendodermal cells from their invagination site at the blastopore. Furthermore, our detailed successive sequence shows: 1) early differentiation of the precheliceral neuroectoderm; 2) the morphogenetic process of inversion and 3) initial invaginations of the opisthosomal epithelium for the respiratory system. CONCLUSIONS: Our improved staging system of development in C. salei development should be of considerable value to future comparative studies of animal development. A dense germ disc is not evident during development in C. salei, but we show that the gastrulation process is similar to that in spider species that do have a dense germ disc. In the opisthosoma, the order of appearance of precursor epithelial invaginations provides evidence for the non-homology of the tracheal and book lung respiratory systems.
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Unmanned Aerial Systems (UAS) are becoming increasingly popular in the public safety sector. While some applications have so far only been envisioned, others are regularly performed in real-life scenarios. Many more fall in between and are actively investigated by research and commercial communities alike. This study reviews the maturity levels, or "market-readiness", of public safety applications for UAS. As individual assessments of all applications suggested in the literature are infeasible due to their sheer number, we propose a novel set of application categories: Remote Sensing, Mapping, Monitoring, Human-drone Interaction, Flying Ad-hoc Networks, Transportation, and Counter UAV Systems. Each category's maturity is assessed through a literature review of contained applications, using the metric of Application Readiness Levels (ARLs). Relevant aspects such as the environmental complexity and available mission time of addressed scenarios are taken into account. Following the analysis, we infer that improvements in autonomy and software reliability are the most promising research areas for increasing the usefulness and acceptance of UAS in the public safety domain.
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The development of the crustacean muscular system is still poorly understood. We present a structural analysis of muscle development in an emerging model organism, the marbled crayfish--a representative of the Cambaridae. The development and differentiation of muscle tissue and its relation to the mesoderm-forming cells are described using fluorescent and non-fluorescent imaging tools. We combined immunohistochemical staining for early isoforms of myosin heavy chain with phallotoxin staining of F-actin, which distinguishes early and more differentiated myocytes. We were thus able to identify single muscle precursor cells that serve as starting points for developing muscular units. Our investigations show a significant developmental advance in head appendage muscles and in the posterior end of the longitudinal trunk muscle strands compared to other forming muscle tissues. These findings are considered evolutionary relics of larval developmental features. Furthermore, we document the development of the muscular heart tissue from myogenic precursors and the formation and differentiation of visceral musculature.
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Astacoidea/embriologia , Embrião não Mamífero/embriologia , Modelos Animais , Desenvolvimento Muscular , Animais , Astacoidea/metabolismo , Diferenciação Celular , Embrião não Mamífero/metabolismo , Imuno-Histoquímica , Microscopia Confocal , Proteínas Musculares/metabolismo , Músculos/citologia , Músculos/embriologia , Músculos/metabolismo , Mioblastos/citologia , Mioblastos/metabolismo , Cadeias Pesadas de Miosina/metabolismoRESUMO
To examine the evolution of development and put it into a phylogenetic context, it is important to have, in addition to a model organism like Drosophila, more insights into the huge diversity of arthropod morphologies. In recent years, the malacostracan crustacean Porcellio scaber Latreille, 1804 has become a popular animal for studies in evolutionary and developmental biology, but a detailed and complete description of its embryonic development is still lacking. Therefore, the embryonic development of the woodlouse P. scaber is described in a series of discrete stages easily identified by examination of living animals and the widely used technique of nuclei staining on fixed specimens. It starts with the first cleavage of the zygote and ends with a hatched manca that eventually leaves the mother's brood pouch. Classical methods like normal light microscopy, scanning electron microscopy and fluorescence microscopy are used, in addition to confocal LCM and computer-aided 3D reconstruction in order to visualise important processes during ontogeny. The purpose of these studies is to offer an easy way to define the different degrees of development for future comparative analyses of embryonic development amongst crustaceans in particular, as well as between different arthropod groups. In addition, several aspects of Porcellio embryonic development, such as the mouth formation, limb differentiations and modifications or the formation of the digestive tract, make this species particularly interesting for future studies in evolutionary and developmental biology.
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Evolução Biológica , Desenvolvimento Embrionário/fisiologia , Isópodes/embriologia , Animais , Embrião não Mamífero/ultraestrutura , Gastrulação/fisiologia , Microscopia Eletrônica de Varredura , Microscopia de FluorescênciaRESUMO
The post-embryonic development of a species of the enigmatic crustacean group Remipedia is described in detail for the first time under various aspects. Applying a molecular approach, we can clearly prove the species identity of the larvae as belonging to Pleomothra apletocheles. We document the cellular level of several larval stages and the differentiation of segments, limbs, and the general body morphology applying the techniques of confocal laser scanning microscopy and scanning electron microscopy. In addition, we document the swimming behavior and the peculiar movements of the naupliar appendages. A comparison of our results with published data on other Crustacea and their larval development tentatively supports ideas about phylogenetic affinities of the Remipedia to the Malacostraca.
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Crustáceos/crescimento & desenvolvimento , Crustáceos/ultraestrutura , Animais , Bahamas , Crustáceos/classificação , Crustáceos/genética , Larva/ultraestrutura , Análise de Sequência de DNARESUMO
The development of macrostomid flatworms is of interest for evolutionary developmental biology research because these taxa combine characteristics of the canonical spiral cleavage pattern with significant deviations from this pattern. One such deviation is the formation of hull cells, which surround the remaining embryonic primordium during early development. Using live observations with a 4D microscope system, histology, and 3D reconstructions, we analyzed the ontogeny of these hull cells in the macrostomid model organism Macrostomum lignano. Our cell lineage analysis allowed us to find the precursors of the hull cells in this species. We discuss the relation between macrostomid development and the development of other spiralians and the question of whether hull cells are homologous within rhabditophoran flatworms.
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Turbelários/embriologia , Animais , Blastômeros/citologia , Turbelários/classificação , Turbelários/citologiaRESUMO
Eusynstyelamides A-C (1-3) were isolated from the Great Barrier Reef ascidian Eusynstyela latericius, together with the known metabolites homarine and trigonelline. The structures of 1-3, with relative configurations, were elucidated by interpretation of their spectroscopic data (NMR, MS, UV, IR, and CD). The NMR data of 1 were found to be virtually identical to that reported for eusynstyelamide (4), isolated from E. misakiensis, indicating that a revision of the structure of 4 is needed. Eusynstyelamides A-C exhibited inhibitory activity against neuronal nitric oxide synthase (nNOS), with IC(50) values of 41.7, 4.3, and 5.8 microM, respectively, whereas they were found to be nontoxic toward the three human tumor cell lines MCF-7 (breast), SF-268 (CNS), and H-460 (lung). Compounds 1 and 2 displayed mild inhibitory activity toward Staphylococcus aureus (IC(50) 5.6 and 6.5 mM, respectively) and mild inhibitory activity toward the C(4) plant regulatory enzyme pyruvate phosphate dikinase (PPDK) (IC(50) values of 19 and 20 mM, respectively).
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Indóis/isolamento & purificação , Indóis/farmacologia , Óxido Nítrico Sintase Tipo I/antagonistas & inibidores , Urocordados/química , Animais , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Humanos , Indóis/química , Concentração Inibidora 50 , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Piruvato Ortofosfato Diquinase/antagonistas & inibidores , Staphylococcus aureus/efeitos dos fármacosRESUMO
We present the first comparative cell lineage analysis of uniramous and biramous limbs of an arthropod, the crustacean Orchestia cavimana. Via single cell labelling of the cells that are involved in limb development, we are able to present the first complete clonal composition of an arthropod limb. We show that the two main branches of crustacean limbs, exopod and endopod, are formed by a secondary subdivision of the growth zone of the main limb axis. Additional limb outgrowths such as exites result from the establishment of new axes. In contrast to general belief, uniramous limbs in Orchestia are not formed by the loss of the exopod but by suppression of the split into exopod and endopod. Our results offer a developmental approach to discriminate between the different kinds of branches of arthropod appendages. This leads to the conclusion that a 'true' biramous limb comprising an endopod and an exopod might have occurred much later in euarthropod evolution than has previously been thought, probably either in the lineage of the Mandibulata or that of the Tetraconata.
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Artrópodes/anatomia & histologia , Crustáceos/anatomia & histologia , Extremidades/anatomia & histologia , Animais , Linhagem Celular , Ectoderma/citologiaRESUMO
During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms of morphogenesis. To study the cellular basis of limb development, we imaged transgenic fluorescently-labeled embryos from the crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days of embryogenesis. The cell lineage of outgrowing thoracic limbs was reconstructed at single-cell resolution with new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that the early limb primordium becomes subdivided into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. Limb-bud formation is associated with spatial modulation of cell proliferation, while limb elongation is also driven by preferential orientation of cell divisions along the proximal-distal growth axis. Cellular reconstructions were predictive of the expression patterns of limb development genes including the BMP morphogen Decapentaplegic.
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Anfípodes/embriologia , Linhagem da Célula , Biologia Computacional/métodos , Extremidades/embriologia , Processamento de Imagem Assistida por Computador/métodos , Morfogênese , Imagem Óptica/métodos , Animais , Fluorescência , Genes Reporter , Software , Análise Espaço-Temporal , Coloração e RotulagemRESUMO
The question of arthropod head segmentation has become one of the central issues in Evolutionary Developmental Biology. The number of theories pertaining to head segments progressively enlarges, old concepts have been revitalized, and nearly every conceivable composition of the arthropod head has at some point received discussion. One contentious issue involves a characteristic mouthpart in crustaceans--the lower lips or the so-called paragnaths. The paragnaths build the posterior border of the mouth region antagonistic to the upper lip--the labrum. We show here the development of the appendage-like structures in the mandibular region of the amphipod crustacean Orchestia cavimana at a high level of cellular resolution. The embryos are examined during development of the mouthparts using in vivo labeling. An invariant cell division pattern of the mandibular segment was detected by 4D-microscopy and a preliminary model for pattern of the first cleavages in the mandibular region created. With this indispensable precondition single ectodermal cells of the grid-like pattern were labeled with DiI--a lipophilic fluorescent dye--to trace cell lineages and determine the clonal composition of the developing mouthparts, especially the mandibular segment. From our data it is evident that the paragnaths are sternal outgrowths of the mandible segment. The assumption of the limb nature of paragnaths and the presence of an additional head segment between the mandibular and the second antennal segments are clearly refuted by our data. Our results show the power of cell lineage and clonal analyses for inferences on the nature, origin and thus homology of morphological structures. With this kind of investigation morphological and gene expression data can be complemented. We discuss notable similarities of paragnath anlagen to those of the hypopharynx complex in myriapods and hexapods. The fact that both structures grow out as two lateral buds in the same region of the mandibular sternite during development, and their important role in the formation of the feeding apparatus as a highly specialized chewing chamber in adults of crustaceans, myriapods, and hexapods argue for the paragnaths/hypopharynx anlagen being an additional potential apomorphy of Mandibulata.
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One of the key components in assessing marine sessile organism demography is determining recruitment patterns to benthic habitats. An analysis of serially deployed recruitment tiles across depth (6 and 12 m), seasons (summer and winter) and space (meters to kilometres) was used to quantify recruitment assemblage structure (abundance and percent cover) of corals, sponges, ascidians, algae and other sessile organisms from the northern sector of the Great Barrier Reef (GBR). Polychaetes were most abundant on recruitment titles, reaching almost 50% of total recruitment, yet covered <5% of each tile. In contrast, mean abundances of sponges, ascidians, algae, and bryozoans combined was generally less than 20% of total recruitment, with percentage cover ranging between 15-30% per tile. Coral recruitment was very low, with <1 recruit per tile identified. A hierarchal analysis of variation over a range of spatial and temporal scales showed significant spatio-temporal variation in recruitment patterns, but the highest variability occurred at the lowest spatial scale examined (1 m-among tiles). Temporal variability in recruitment of both numbers of taxa and percentage cover was also evident across both summer and winter. Recruitment across depth varied for some taxonomic groups like algae, sponges and ascidians, with greatest differences in summer. This study presents some of the first data on benthic recruitment within the northern GBR and provides a greater understanding of population ecology for coral reefs.