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Background: Migraine is a common type of primary headache with disabling brain dysfunction. It has been found that pituitary adenylate cyclase activating polypeptide (PACAP) is involved in the pathogenesis of migraine, however, the role of PACAP and its receptors in chronic migraine remains unclear. Therefore, the present study aimed to explore the changes of PACAP and its receptors in different duration after recurrent dural inflammation soup stimulations and to investigate the co-expression between PACAP and calcitonin gene-related peptide (CGRP). Methods: Adult male rats were implanted with cannula surrounding superior sagittal sinus, which was followed by dural infusion of inflammatory soup (IS) or normal saline (NS). The rats were randomly divided into 4 groups (n = 8 for each group): IS stimulation for seven days (IS-7 group), IS stimulation for 14 days (IS-14 group), IS stimulation for 21 days (IS-21 group), and NS control for 21 days (CON group). The facial mechanical withdrawal threshold was daily measured during the whole experiment. The behavioral changes (ipsilateral and bilateral face grooming behavior) in a plastic cage of rats were observed and recorded. The expression of PACAP, its receptors (PAC1, VPAC1, VPAC2), and CGRP in the trigeminal ganglia (TG) and the trigeminal nucleus caudalis (TNC) was examined by immunohistochemistry. Immunofluorescence was used to explore the co-expression of PACAP, PAC1 receptor, and CGRP after repeated IS administration in the TG. Results: The ipsilateral facial grooming time of IS-21 group displayed an apparent increase than CON group after repeated stimulation on day 2, while significant differences were observed on day 14. No differences were found between the IS-21 and CON group in bilateral facial grooming. Dural IS stimulation induced a significantly decrease in facial mechanical withdrawal thresholds. PACAP positive cells in the regions of TNC were gradually decreased with the IS days increasing. PACAP and PAC1 receptor expression in the TG had a trend of increasing first and then decreasing. There was no significant difference in expression of VPAC1 and VPAC2 in the TG and the TNC. Immunofluorescence showed that PACAP was mainly expressed in TG neurons. PACAP and PAC1 receptor co-expression decreased gradually after repetitive IS stimulation. While the co-expression between PACAP and CGRP reached the peak in IS-7 group after repetitive IS stimulation, and then decreased. Conclusions: This study demonstrated that repetitive chemical stimulations induced a gradual decrease of PACAP in the TNC, while the PACAP and PAC1 receptor expression in TG showed dynamical changes of increasing first and then decreasing after repeated IS administration. These results suggested exhaustion of PACAP could be involved in the duration of chronic migraine and implied PACAP may contribute to the pathology of migraine through the PAC1 receptor, which was associated with CGRP.
Assuntos
Transtornos de Enxaqueca , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase , Ratos , Masculino , Animais , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/metabolismo , Receptores de Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/metabolismo , Peptídeo Relacionado com Gene de Calcitonina/metabolismo , Ratos Sprague-Dawley , Estimulação QuímicaRESUMO
Varicella-zoster virus (VZV) lurks in cranial nerves and other brain ganglias after infection. Because middle cerebral artery (MCA) receives the ipsilateral trigeminal ganglia afferent innervations, the reactivated VZV infects the adventitia and intima of cerebral artery wall probably through this way and causes vascular inflammation, finally resulting in artery remodeling, vessel occlusion, and ischemia. In fact, there is a growing clinical recognition that there is an association between VZV reactivation and subsequent stroke. Here, we showed a case of ischemic stroke secondary to varicella-zoster virus meningoencephalitis and reviewed the literature to emphasize the importance of VZV-associated vasculopathy.
Assuntos
Encefalite por Varicela Zoster , Herpes Zoster , AVC Isquêmico , Meningoencefalite , Acidente Vascular Cerebral , Encefalite por Varicela Zoster/complicações , Herpes Zoster/complicações , Herpesvirus Humano 3 , Humanos , Meningoencefalite/complicaçõesRESUMO
Migraine is the seventh most disabling disorder globally, with prevalence of 11.7% worldwide. One of the prevailing mechanisms is the activation of the trigeminovascular system, and calcitonin gene-related peptide (CGRP) is an important therapeutic target for migraine in this system. Recent studies suggested an emerging role of pituitary adenylate cyclase-activating peptide (PACAP) in migraine. However, the relation between CGRP and PACAP and the role of PACAP in migraine remain undefined. In this study, we established a novel repetitive (one, three, and seven days) electrical stimulation model by stimulating dura mater in conscious rats. Then, we determined expression patterns in the trigeminal ganglion and the trigeminal nucleus caudalis of the trigeminovascular system. Electrical stimulation decreased facial mechanical thresholds, and the order of sensitivity was as follows: vibrissal pad >inner canthus >outer canthus (P < 0.001). The electrical stimulation group exhibited head-turning and head-flicks (P < 0.05) nociceptive behaviors. Importantly, electrical stimulation increased the expressions of CGRP, PACAP, and the PACAP-preferring type 1 (PAC1) receptor in both trigeminal ganglion and trigeminal nucleus caudalis (P < 0.05). The expressions of two vasoactive intestinal peptide (VIP)-shared type 2 (VPAC1 and VPAC2) receptors were increased in the trigeminal ganglion, whereas in the trigeminal nucleus caudalis, their increases were peaked on Day 3 and then decreased by Day 7. PACAP was colocalized with NEUronal Nuclei (NeuN), PAC1, and CGRP in both trigeminal ganglion and the trigeminal nucleus caudalis. Our results demonstrate that the repetitive electrical stimulation model can simulate the allodynia during the migraine chronification, and PACAP plays a role in the pathogenesis of migraine potentially via PAC1 receptor.
Assuntos
Peptídeo Relacionado com Gene de Calcitonina/metabolismo , Terapia por Estimulação Elétrica/métodos , Transtornos de Enxaqueca/terapia , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/metabolismo , Receptores de Polipeptídeo Hipofisário Ativador de Adenilato Ciclase/metabolismo , Núcleo Inferior Caudal do Nervo Trigêmeo/fisiologia , Animais , Feminino , Masculino , Transtornos de Enxaqueca/fisiopatologia , Nociceptividade/efeitos dos fármacos , Dinâmica não Linear , Fosfopiruvato Hidratase/metabolismo , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Estatísticas não Paramétricas , Fatores de TempoRESUMO
The design and manufacture of high activity and thermal stability catalysts with minimal precious metal loading is essential for deep degradation of volatile organic compounds (VOCs). In this paper, a novel single-atom Pt-CeO2/Co3O4 catalyst with ultra-low Pt loading capacity (0.06 wt%, denoted as 0.06Pt-SA) was fabricated via one-step co-precipitation method. The 0.06Pt-SA exhibited excellent toluene degradation activity of T90 = 169 °C, matched with the nanoparticle Pt-supported CeO2/Co3O4 catalyst with more than six times higher Pt loading (0.41 wt%, denoted as 0.41Pt-NP). Moreover, the ultra-long durability (toluene conversion remains 99% after 120 h stability test) and excellent toluene degradation ability in a wide space speed range of 0.06Pt-SA were superior to that of 0.41Pt-NP catalyst. The excellent performance was derived from the strong metal-support interaction (SMSI) between the single atomic Pt and the carrier, which induced more Pt0 and Ce3+ for oxygen activation and more Co3+ for toluene removal. The in situdiffuse reflectance infrared spectroscopy (DRIFTS) experiments confirmed that the conversion of intermediates was accelerated in the reaction process, thereby promoting the toluene degradation. Our results should inspire the exploitation of noble single-atomic modification strategy for developing the low cost and high performance VOCs catalyst.
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Bismuth vanadate (BiVO4) is a prospective candidate for photoelectrochemical (PEC) water oxidation, but its commercial application is limited due to the serious surface charge recombination. In this work, we propose a novel and effective electrochemical reduction strategy combined with co-catalyst modification to manipulate the surface states of the BiVO4 photoanode. Specifically, an ultrathin amorphous structure is formed on the surface of BiVO4 after electrochemical reduction ascribed to the breaking of the surface metal-O bonds. Photoelectrochemical measurements and first-principles calculation show that the electrochemical reduction treatment can effectively reduce the surface energy, thereby passivating the recombined surface states (r-ss) and increasing the mobility of photogenerated holes. In addition, the FeOOH co-catalyst layer further increases the intermediate surface states (i-ss) of BiVO4, stabilizes the surface structure and enhances its PEC performance. Benefiting from the superior charge transfer efficiency and the excellent water oxidation kinetics, the -0.8/BVO/Fe photoanode achieves 2.02 mA cm-2 photocurrent at 1.23 VRHE (2.4 times that of the original BiVO4); meanwhile, the onset potential shifts 90 mV to the cathode. These results provide a new surface engineering tactic to modify the surface states of semiconductor photoanodes for high-efficiency PEC water oxidation.
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BACKGROUND: Cerebrovascular complications after adult-onset varicella-zoster virus (VZV) encephalitis have been increasingly recognized. The aim of this study was to analyze clinical and neuroimaging findings, treatment and outcome of these patients. METHODS: Literature review from January 2000 to December 2019. We searched for studies published in PubMed, Embase and Chinese Biomedical Literature Database. Clinical symptoms, neuroimaging findings, treatment and outcome were evaluated. RESULTS: We analyzed 31 articles with a total of adult-onset 34 cases, including 25 cases of ischemic stroke, 6 of intracerebral hemorrhage and 3 with venous sinus thrombosis. Ischemic stroke was the major complication after VZV encephalitis accounting of 73.35%. There were more males than females in ischemia or venous sinus thrombosis groups. The middle-aged was prone to cerebral infarction, the elderly was for cerebral hemorrhage, and the young was for venous sinus thrombosis. Cognitive impairment was the most common symptom either in the ischemic group or hemorrhagic group. The lesions of VZV-associated cerebral infarction or hemorrhage were multifocal and mostly involved in the parietal lobe, followed by frontal or temporal lobes. Venous sinus thrombosis was common in the transverse sinus. Multiple stenosis of the anterior and posterior circulation vessels was found. A 60.87% of the patients with antiviral treatment in the ischemic group had favorable prognosis. All patients with anticoagulant therapy in venous sinus thrombosis group improved well; however, 60% of the patients with intracerebral hemorrhage had a poor prognosis or died. CONCLUSION: Ischemic stroke was the majority of cerebrovascular complications after VZV encephalitis, which mainly occurred in middle-aged men. The lesions of VZV-associated cerebral infarction or hemorrhage were multifocal and did not accord with the characteristics of cerebrovascular diseases induced by atherosclerosis. The patients with venous sinus thrombosis had a relatively good prognosis. When the patient represents with some neurological symptoms about one month after VZV encephalitis, and multiple lesions probably induced by vasculitis are showed in neuroimaging, cerebrovascular complications related to VZV infection should be considered.
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Extracting atmospheric moisture for freshwater production is an appealing way to mitigate the global water crisis. However, the low moisture sorption capacity and high desorption temperature are the major bottlenecks for efficient atmospheric water harvesting. Herein, we develop a transition metal super-hygroscopic hydrogel by an economical strategy, which is constructed through a facile coordination between metal salts and ethanolamine. When the empty electron orbital of the metal ion is coordinated with the lone electron pair of nitrogen or oxygen atom, the water active sorption site is formed. A single water layer is bonded on the sites by a coordination effect, followed by physical interaction with water to form multi-layer structures. The Fe and Co ions in the hydrogel function as dual sorption sites to capture moisture, which can harvest additional water by the synergistic effect of bimetals. As a result, the bimetal hydrogel contributes to a high water uptake of 5.22 g g-1 at 95% RH, triggering the desorption process by one solar intensity due to its low desorption temperature (≤50 °C).
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BACKGROUND: To identify differences in allodynia and grooming behaviours between rat models of either repeated dural nociception with inflammatory soup (IS) or infraorbital nerve chronic constriction injury (IoN-CCI). METHODS: Repeated dural nociception was induced via the application of IS to the dural meninges and IoN-CCI was applied to model neuropathic pain. All surgeries were performed on the right side and a sham operation was performed on the control group. Mechanical and thermal withdrawal thresholds were tested on different facial areas and hindpaw during the interictal period and grooming behaviours were recorded. RESULTS: A significant decreases was found in the mechanical withdrawal thresholds of the bilateral vibrissa pad and right periorbital area in both the IS and the IoN-CCI groups, but only in the left periorbital area of the IS group. Hindpaw thermal allodynia was evident only in the IS group. Ipsilateral hindpaw grooming behaviour increased in the IS group and facial grooming behaviour increased in the IoN-CCI group. CONCLUSIONS: Repeated dural nociception induced by IS and IoN-CCI in rats effectively simulated chronic migraine (CM) and trigeminal neuralgia (TN), respectively. The IS group exhibited a wider range of allodynia than the IoN-CCI group, but further studies are necessary to determine underlying mechanisms.