EGFR-MEK1/2 cascade negatively regulates bactericidal function of bone marrow macrophages in mice with Staphylococcus aureus osteomyelitis.

The ability of Staphylococcus aureus (S. aureus) to survive within macrophages is a critical strategy for immune evasion, contributing to the pathogenesis and progression of osteomyelitis. However, the underlying mechanisms remain poorly characterized. This study discovered that inhibiting the MEK1/2 pathway reduced bacterial load and mitigated bone destruction in a mouse model of S. aureus osteomyelitis. Histological staining revealed increased phosphorylated MEK1/2 levels in bone marrow macrophages surrounding abscess in the mouse model of S. aureus osteomyelitis. Activation of MEK1/2 pathway and its roles in impairing macrophage bactericidal function were confirmed in primary mouse bone marrow-derived macrophages (BMDMs). Transcriptome analysis and in vitro experiments demonstrated that S. aureus activates the MEK1/2 pathway through EGFR signaling. Moreover, we found that excessive activation of EGFR-MEK1/2 cascade downregulates mitochondrial reactive oxygen species (mtROS) levels by suppressing Chek2 expression, thereby impairing macrophage bactericidal function. Furthermore, pharmacological inhibition of EGFR signaling prevented upregulation of phosphorylated MEK1/2 and restored Chek2 expression in macrophages, significantly enhancing S. aureus clearance and improving bone microstructure in vivo. These findings highlight the critical role of the EGFR-MEK1/2 cascade in host immune defense against S. aureus, suggesting that S. aureus may reduce mtROS levels by overactivating the EGFR-MEK1/2 cascade, thereby suppressing macrophage bactericidal function. Therefore, combining EGFR-MEK1/2 pathway blockade with antibiotics could represent an effective therapeutic approach for the treatment of S. aureus osteomyelitis.

Young human plasma-derived extracellular vesicles rescue and reactivate IL-1ß and TNF-α treated chondrocytes.

Osteoarthritis (OA) is a degenerative disease that affects millions of individuals worldwide. Despite its prevalence, the exact causes and mechanisms behind OA are still not fully understood, resulting in a lack of effective treatments to slow down or halt disease progression. Recent research has discovered that extracellular vesicles (EVs) present in the circulation of young mice have a remarkable ability to activate musculoskeletal stem cells in elderly mice. Conversely, EVs derived from elderly mice do not exhibit the same potential, indicating that EVs obtained from young individuals may hold promise to activate aging cells in degenerative tissue. However, it remains unknown whether EVs derived from young individuals can also address cartilage degeneration caused by aging. In this study, we first evaluated EVs derived from young human plasma (YEVs) and EVs derived from old human plasma (OEVs) in an in vitro experiment using chondrocytes. The results revealed that YEVs effectively stimulated chondrocyte proliferation and migration, while OEVs from old plasma did not exhibit a similar effect. Given that OA represents a more complex inflammatory microenvironment, we further determine whether the benefits of YEVs on chondrocytes can be maintained in this context. Our findings indicate that YEVs have the ability to positively regulate chondrocyte function and protect them against apoptosis induced by IL-1ß and TNF-α in an in vitro OA model. Furthermore, we discovered that lyophilized EVs could be stored under mild conditions without any alterations in their physical characteristics. Considering the exceptional therapeutic effects and the wide availability of EVs from young plasma, they hold significant promise as a potential approach to activate chondrocytes and promote cartilage regeneration in early-stage OA.

Selenium-Doped Seeded Growth of Truncated Octahedral Gold Nanocrystals with Surface Concavities for Surface-Enhanced Raman Spectroscopy.

Concave nanocrystals stand out as a testament to the importance of the nanoscale morphology in dictating the functional properties of materials. In this report, we introduce a facile synthesis method for producing gold (Au) nanocrystals with a truncated octahedral morphology that features surface concavities (Au CNTOs). The incorporation of selenium (Se) doping into the truncated octahedral Au seeds was essential for their enlargement and the formation of concave structures. By simply adjusting the quantity of seeds, we could control the size of the nanocrystals while maintaining their distinctive morphology and surface concavity. The formation mechanism suggests that Se doping likely passivates the side faces, thereby slowing growth and promoting atomic deposition at the edges and corners. The resulting Se-doped Au CNTOs exhibited strong localized surface plasmon resonance (LSPR) absorptions in the visible spectrum and the SERS performance of their assemblies was demonstrated through crystal violet detection, reaching enhancement factors around 105. This study presents an innovative approach to synthesizing concave Au nanocrystals through the incorporation of selenium during a seeded growth process, offering insights into the strategic design of plasmonic nanostructures.

Improving Catalytic Performance via the Synergy of Tensile Lattice Strain and Plasmonic Enhancement in Defective AuPd@Pd Short Nanowires.

The synthesis of bimetallic nanocatalysts with strained crystal lattices has attracted considerable interest. This is because, beyond the electronic structure modifications realized through elemental doping, the strain effect offers an extra mechanism to fine-tune the electronic structures, thereby possibly improving the catalytic performances. We present a method for constructing defective AuPd@Pd short nanowires, achieved through a controlled galvanic replacement reaction between short AuCu nanowires and Pd precursors. Advanced structural analyses using spherical aberration-corrected transmission electron microscopy (AC-TEM) validated the expanded crystal lattice on the nanowire surface and also demonstrated pronounced plasmonic absorption in the UV-vis region. Leveraging both plasmonic absorption and strain effects, the AuPd@Pd short nanowires displayed a higher apparent rate constant compared to Pd nanoparticles. Integrating molecular dynamic simulations with density functional theory calculations revealed that the tensile strain on AuPd@Pd short nanowires benefited the catalytic activity by elevating the d-band center, thereby intensifying the adsorption of p-nitrophenol. The current research introduces a unique method for synthesizing noble metal nanocrystals with specific dimensions and elucidates the rational development of high-performance plasmonic nanocatalysts through synergistic exploitation of the beneficial strain effect.

A multi-dimensional photodetector based on an α-MoO3 grating and graphene.

Integration of multi-dimensional optical information enhances the recognition and anti-interference capabilities of the detection system, allowing for better adaptation to complex environments. Therefore, this technology represents a crucial developmental pathway for the future of infrared optical detectors. In this study, a dual-band polarization photodetector based on a two-dimensional α-MoO3 grating structure is proposed. The structure utilizes the special dispersion property of the α-MoO3 material to excite the localized plasmon resonance, which generates narrowband high absorption peaks with Q-factors as high as 110.24 and 92.65, with peaks close to 1 under TM and TE polarized waves, respectively. The direct measurement of multi-dimensional optical information including intensity, spectrum and polarization states is realized. By adjusting the structural parameters, polarization-dependent dual-band detection can be achieved. Meanwhile, the introduction of graphene material realizes the electronically tunable function of the device. This study provides unexplored strategies for realizing more efficient, flexible and versatile dual-band polarization wave detection.

Coupling polaritons in near-field radiative heat transfer between multilayer graphene/vacuum/α-MoO3/vacuum heterostructures.

Both materials and structures can significantly affect radiative heat transfer, which is more pronounced in the near-field regime of two-dimensional and hyperbolic materials, and has promising prospects in thermophotovoltaics, radiative cooling, and nanoscale metrology. Hence, it is important to investigate the near-field radiative heat transfer (NFRHT) in complicated heterostructures consisting of two-dimensional and hyperbolic materials. Recent studies have reported that adding vacuum layers to multilayer structures can effectively enhance the NFRHT. Take the case of multilayer graphene/α-MoO3 heterostructures: the effect of vacuum layers on these heterostructures has not been studied, and hence investigations on adding vacuum layers between graphene and α-MoO3 layers should be emphasized. In this work, we conduct an investigation of the NFRHT between multilayer graphene/vacuum/α-MoO3/vacuum heterostructures. Compared to unit graphene/α-MoO3 heterostructures without vacuum layers, it is found that NFRHT between the heterostructures with vacuum layers can be suppressed to 49.1% when the gap distance is 10 nm, and can be enhanced to 16.3% when the gap distance is 100 nm. These phenomena are thoroughly explained by the coupling of surface plasmon polaritons and hyperbolic phonon polaritons. Energy transmission coefficients and spectral heat flux are analysed during the calculations changing chemical potentials of graphene, thicknesses of vacuum layers, and α-MoO3 layers. This study is expected to provide guidance in implementing the thermal management of reasonable NFRHT devices based on graphene/α-MoO3 heterostructures.

A tungsten-based metamaterial emitter for solar thermophotovoltaic systems.

Solar thermophotovoltaic systems are capable of showing efficient photoelectric conversion and are expected to surpass the Shockley-Queisser limit, owing to the spectrum-selective functionality of metamaterial selective emitters. Generally, metamaterial emitters are manufactured from multifarious materials, which also makes their manufacturing process complicated. Here, we propose a tungsten-only emitter composed of two rectangular bars with different widths and heights arranged in a cruciform structure, featuring a rectangular cavity at the top. Results from the simulations reveal that the emissivity of the metamaterial emitter exceeds 90% at the wavelength of 950-1590 nm and drops below 20% for wavelengths exceeding 2025 nm, which can effectively match GaSb photovoltaic cells. The outstanding emission performance is attributed to the coupling effect of surface plasmon resonance, cavity resonance and guided mode resonance, as evidenced by the analysis of electric and magnetic fields. We also explored the radiation spectrum in the 500-2500 K temperature range and found that it performed best at 1400 K. It is concluded that the emission performance is slightly affected by structural parameters and angles. This study presents a meaningful exploration of efficient solar utilization.

PD-1/PD-L1 blockade is a potent adjuvant in treatment of Staphylococcus aureus osteomyelitis in mice.

There is no effective therapy for implant-associated Staphylococcus aureus osteomyelitis, a devastating complication after orthopedic surgery. An immune-suppressive profile with up-regulated programmed cell death 1/programmed death ligand 1 (PD-1/PD-L1) was identified based on our transcriptional data (GEO: GSE166522) from a mouse model of S. aureus osteomyelitis. PD-1/PD-L1 expression was up-regulated mainly in F4/80+ macrophages surrounding the abscess in S. aureus-infected bone. Mechanistically, PD-1/PD-L1 activated mitophagy to suppress production of mitochondrial reactive oxygen species (ROS), suppressing the bactericidal function of macrophages. Using neutralizing antibodies for PD-L1 or PD-1, or knockout of PD-L1 adjuvant to gentamicin markedly reduced mitophagy in bone marrow F4/80+ cells, enhanced bacterial clearance in bone tissue and implants, and reduced bone destruction in mice. PD-1/PD-L1 expression was also increased in the bone marrow from individuals with S. aureus osteomyelitis. These findings uncover a so far unknown function of PD-1/PD-L1-mediated mitophagy in suppressing the bactericidal function of bone marrow macrophages.

Greenhouse cultivation enhances pesticide bioaccumulation in cowpeas following repeated spraying.

Understanding pesticide residue patterns in crops is important for ensuring human health. However, data on residue accumulation and distribution in cowpeas grown in the greenhouse and open field are lacking. Our results suggest that acetamiprid, chlorantraniliprole, cyromazine, and thiamethoxam residues in greenhouse cowpeas were 1.03-15.32 times higher than those in open field cowpeas. Moreover, repeated spraying contributed to the accumulation of pesticide residues in cowpeas. Clothianidin, a thiamethoxam metabolite, was detected at 1.04-86.00 µg/kg in cowpeas. Pesticide residues in old cowpeas were higher than those in tender cowpeas, and the lower half of the plants had higher pesticide residues than did the upper half. Moreover, pesticide residues differed between the upper and lower halves of the same cowpea pod. Chronic and acute dietary risk assessments indicated that the human health risk was within acceptable levels of cowpea consumption. Given their high residue levels and potential accumulation, pesticides in cowpeas should be continuously assessed.

Ultrasensitive optical modulation in hybrid metal-perovskite and metal-graphene metasurface THz devices.

Implementation of efficient terahertz (THz) wave control is essential for THz technology development for applications including sixth-generation communications and THz sensing. Therefore, realization of tunable THz devices with large-scale intensity modulation capabilities is highly desirable. By integrating perovskite and graphene with a metallic asymmetric metasurface, two ultrasensitive devices for dynamic THz wave manipulation through low-power optical excitation are demonstrated experimentally here. The perovskite-based hybrid metadevice offers ultrasensitive modulation with a maximum modulation depth for the transmission amplitude reaching 190.2% at the low optical pump power of 5.90 mW/cm2. Additionally, a maximum modulation depth of 227.11% is achieved in the graphene-based hybrid metadevice at a power density of 18.87 mW/cm2. This work paves the way toward design and development of ultrasensitive devices for optical modulation of THz waves.

Epsilon-near-zero characteristics of near-field radiative heat transfer between α-MoO3 slabs.

Epsilon-near-zero (ENZ) materials, which manifest a wealth of exotic optical characteristics, have attracted significant research interest in recent years. However, these characteristics have rarely been considered in the study of near-field radiative heat transfer (NFRHT). In this work, we investigated the ENZ characteristics of the NFRHT between two symmetric biaxial α-MoO3 slabs. The numerical results show that the NFRHT is greatly enhanced around the ENZ frequency of 1.604 × 1014 rad s-1. Notably, near the other two ENZ frequencies (1.832 × 1014 rad s-1 and 1.891 × 1014 rad s-1), only the NFRHT between α-MoO3 slabs of certain thicknesses is enhanced. The reasons can be explained by the fact that the VHPs can be excited in almost all azimuthal angles at angular frequencies of 1.604 × 1014 rad s-1 and 1.891 × 1014 rad s-1. For the ENZ frequency of 1.832 × 1014 rad s-1, the VHPs can be excited at the top and bottom, while the SHPs excited at the left and right sides. It is worth noting that both the hyperbolic and ENZ characteristics affect the NFRHT between α-MoO3 slabs. Moreover, the excitation is strongly dependent on the thickness of the slab. Our findings contribute to understanding the physical mechanisms underlying the characteristics of the NFRHT at ENZ frequencies.

Comparative analysis of two models for the optical response of a thin slab: a film of finite thickness versus a surface current sheet.

An accurate description of the electromagnetic properties of materials is fundamental to optical and electric devices. As a current research hotspot, thin slabs generally are modeled as a film of finite thickness with a dielectric function. However, inspired by two-dimensional materials, thin slabs can be regarded as surface current sheets with conductivity. Due to the convenience of the latter in simplifying the calculations, it becomes increasingly significant to determine the equivalent conditions of the two models. In this work, we compare the differences between the thin film and surface current models in calculating the transmissivity, reflectivity, and absorptivity of a SiC film. For normal incidence, the difference between the calculations of the two models is only non-negligible when the thickness is large (500 nm), because of the invalidation of surface current models and the excitation of Fabry-Perot resonance. In particular, we derive analytical formulas for the relative error in transmittance versus phase difference, which can be used to predict the difference between the two models as a function of film thickness. For oblique incidence, the two models have significant differences in the vicinity of epsilon-near-zero (ENZ) frequency. The excitation of the Berreman leaky mode in a thin film model causes a narrow blank absorption peak close to the ENZ frequency. However, we found that the surface current model is unable to form this resonance mode and further demonstrate it theoretically. In addition, it is found that the two models are equivalent in the case of a transverse electric wave even though the incidence is oblique. This work can enhance the awareness of the light-matter interaction and open unprecedented avenues for designing ultrathin optical devices.

Pattern-free solar absorber driven by superposed Fabry-Perot resonances.

Solar absorbers, which harvest solar irradiation in the form of heat, have promising prospects in electricity, heating, desalination, and energy storage. However, in previous work, absorbers are usually designed as nanostructures involving photolithography to obtain a superior spectral absorption performance, which inevitably increases the cost and complexity of fabrication. Here, we propose a pattern-free absorber consisting of the TiN-SiO2-based multilayer structure for effective solar energy utilization. Numerical results show that the maximum average spectral absorption of a 3-cell multilayer structure is up to 93.5% at wavelengths of 0.3-2.5 µm. The underlying physical mechanism can be explained by the coupling of superposed Fabry-Perot (FP) resonances and the intrinsic absorption of TiN, which is further confirmed via the electric field and power dissipation density distributions. The effect of the geometric parameters and materials of multilayer structures on the spectral absorption performance is investigated. Moreover, we discuss the influence of the incidence angle on solar absorbers and demonstrate that an average spectral absorption of more than 80% can be obtained even at a large incidence angle of 60°. Finally, when the number of cells in the multilayer structure is increased to 6, the average spectral absorption can reach 96%. The findings in this work will deepen the understanding of FP resonance and pave a novel path for efficient solar thermal energy utilization.

Transparent spacecraft smart thermal control device based on VO2 and hyperbolic metamaterials.

Effective spacecraft thermal control technologies are essential to avoid undesirable effects caused by extreme thermal conditions. In this paper, we demonstrate a transparent smart radiation device (TSRD) based on vanadium dioxide (VO2) and a hyperbolic metamaterial (HMM) structure. Using the topological transition property of HMM, high transmission in the visible band and high reflection in the infrared can be achieved simultaneously. The variable emission essentially originates from the phase change material VO2 film. Due to the high reflection of HMM in the infrared band, it can form Fabry-Pérot (FP) resonance with the VO2 film after adding the dielectric layer SiO2, which further enhances the emission modulation. Under optimized conditions, solar absorption can be reduced to 0.25, while emission modulation can reach 0.44 and visible transmission can be up to 0.7. It can be found that the TSRD can simultaneously achieve infrared variable emission, high visible transparency and low solar absorption. The HMM structure instead of traditional metal reflectors offers the possibility to achieve high transparency. In addition, the formation of FP resonance between the VO2 film and HMM structure is the key to achieving variable emission. We believe that this work can not only provide a new approach for the design of spacecraft smart thermal control devices, but also show great potential for application in spacecraft solar panels.

A dual-band hydrogen sensor based on Tamm plasmon polaritons.

Optical hydrogen sensors possess significant potential in various fields, including aerospace and fuel cell applications, which is due to their compact design and immunity to electromagnetic interference. However, commonly used sensors mostly use single-band sensing, which increases the risk of inaccurate measurements due to environmental interference or operational errors. To address this issue, this study proposes a dual-band hydrogen sensor comprising a Pd metal layer, a dielectric spacer layer, a defect layer, and a photonic crystal. By leveraging the interaction between the defect mode in the excitonic microcavity structure and the Tamm plasmon polaritons (TPPs) and Fabry-Perot (FP) resonances, the structure simultaneously generates two near-zero resonance valleys in the visible wavelength range. By adjusting the thickness of the defect layer, the coupling effect of the defect mode and TPPs together with FP resonance respectively is optimized. When the thickness is 0.27 µm, the sensitivities of the Tamm resonance band and FP resonance band are 239 and 21 RIU-1, respectively. Compared with the common sensors with a single band, its low-sensitivity wavelength can be used as a reference to assist the high-sensitivity wavelength for sensing. In addition, we find that the proposed sensor, through calculation, has good fault tolerance for both the thickness of the defect layer and the incident light angle. This study demonstrates a dual-band hydrogen sensor with TPPs, which is important for exploring new optical hydrogen sensors.

A polarization-dependent perfect absorber with high Q-factors enabled by Tamm phonon polaritons in hyperbolic materials.

As a natural biaxial hyperbolic material, α-phase molybdenum trioxide (α-MoO3) is highly anisotropic, making it an ideal candidate for polarization-dependent devices. Herein, using a Tamm configuration where one-dimensional photonic crystal is coated on an α-MoO3 substrate separated by a dielectric interlayer, we demonstrate the perfect absorption effect in the mid-infrared band governed by Tamm phonon polaritons. The resultant absorption peak exhibits an ultra-narrow bandwidth due to the polaritonic resonance with a high quality factor of up to 181. By varying the thickness of the interlayer, we demonstrate that near-unity absorption resonances can be tuned to a wider range of wavelengths. In addition, due to the in-plane anisotropy of α-MoO3, the device exhibits an outstanding polarization-dependent absorption performance, rendering it highly useful for various applications. Also, we show that the electronic tunability of the device is through addition of a graphene monolayer. These excellent results suggest that the designed structure could be promising in applications such as infrared absorbers, polarization detectors, sensors and energy harvesting devices.

Enhanced chirality induced in a composite structure consisting of α-MoO3 film and a silver metasurface.

Chiral structures have been widely used in many fields, such as biosensing and analytical chemistry. In this paper, the chiral response of a composite structure consisting of α-M o O 3 film and a silver (Ag) metasurface is studied. First, the effect of the thickness of α-M o O 3 film on the circular dichroism (CD) is discussed, and it is found that CD can reach 0.93 at a wavelength of 9.6 µm when the thickness of α-M o O 3 film is 6.075 µm. To better understand the physical mechanism, we analyze the transverse electric and transverse magnetic wave components in the transmitted wave for the whole structure and each layer. One can see that the strong chirality of the structure is attributed to the polarization conversion of α-M o O 3 film and the selective transmissivity of Ag ribbons. In addition, the influence of the filling factor of the Ag ribbons on chirality is also studied. This work combines hyperbolic material α-M o O 3 with Ag ribbons to enhance CD. Also, it provides greater freedom in the tuning of chirality. We believe that this work not only deepens the understanding of the chiral response of anisotropic materials, but also gives promise for its applications in the fields of polarization optics and biosensing.

Gradient index effect assisted anisotropic broadband absorption in α-MoO3 metamaterial.

As an excellent natural hyperbolic material (HM), α-M o O 3 has a larger hyperbolic bandwidth and longer polariton lifetime than other HMs, which makes it an ideal candidate for broadband absorbers. In this work, we theoretically and numerically investigated the spectral absorption of an α-M o O 3 metamaterial using the gradient index effect. The results show that the absorber has an average spectral absorbance of 99.99% at 12.5-18 µm at transverse electric polarization. When the incident light is transverse magnetic polarization, the broadband absorption region of the absorber is blueshifted, and a similar strong absorption is achieved at 10.6-12.2 µm. By simplifying the geometric model of the absorber using equivalent medium theory, we find that the broadband absorption is caused by the refractive index matching of the metamaterial to the surrounding medium. The electric field and power dissipation density distributions of the metamaterial were calculated to clarify the location of the absorption. Moreover, the influence of geometric parameters of pyramid structure on broadband absorption performance was discussed. Finally, we investigated the effect of polarization angle on the spectral absorption of the α-M o O 3 metamaterial. This research contributes to developing broadband absorbers and related devices based on anisotropic materials, especially in solar thermal utilization and radiation cooling.

Optical axis-driven tunable Brewster effect in anisotropic materials.

The Brewster effect, which is known as a notable physical law, has promising prospects in perfect absorption and angular selectivity transmission. The Brewster effect in isotropic materials has been investigated extensively in previous works. However, the research on anisotropic materials has been rarely carried out. In this work, we theoretically investigate the Brewster effect in quartz crystals with tilted optical axes. The conditions for the occurrence of the Brewster effect in anisotropic materials are derived. The numerical results show that by changing the orientation of the optical axis, we have effectively regulated the Brewster angle of crystal quartz. The reflection of crystal quartz versus the wavenumber and incidence angle at different tilted angles is studied. In addition, we discuss the effect of the hyperbolic region on the Brewster effect of crystal quartz. The Brewster angle negatively correlates with the tilted angle when the wavenumber is 460c m -1 (Type-II). In contrast, when the wavenumber is 540c m -1 (Type-I), the Brewster angle positively correlates with the tilted angle. Finally, the relationship between the Brewster angle and wavenumber at different tilted angles is investigated. The findings in this work will broaden the research field of crystal quartz and open the door for tunable Brewster devices based on anisotropic materials.

Dissipation dynamics and comparative dietary exposure assessment of mefentrifluconazole in rice.

A fast and sensitive analytical method based on UHPLC coupled with tandem mass spectrometry was established to investigate the dissipation and final residual amounts of mefentrifluconazole in rice, and dietary risk to consumers was evaluated. The method provided good linearity (R2 ≥ 0.9979), accuracy (recovery range, 79.0-101.5%), precision (relative standard deviation range, 1.3-13.9%), and sensitivity (limit of quantification, 0.005 mg/kg). The dissipation dynamics of mefentrifluconazole in rice followed first-order kinetics, with half-lives of 2.8-16.6 days. The final residues of mefentrifluconazole in various samples of harvested brown rice ranged from less than the limit of quantification to 0.092 mg/kg, the latter value being higher than the maximum residue limit recommended by the European Union. Comparative dietary exposure of mefentrifluconazole was assessed using field data and Chinese dietary patterns for different genders, regions, and age data. Although the results showed acceptable levels of risk for both acute exposure (the percentage of the acute reference dose ≤ 0.7483%) and chronic dietary intake (the percentage of acceptable daily intake ≤ 31.8516%), more studies of children are needed because they are at higher risk than other groups. This work provides the necessary data for registering and establishing the maximum residue limit for mefentrifluconazole in rice in China and reveals the potential risks to different groups of long-term application of mefentrifluconazole to rice and other crops.