RESUMO
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) has become a powerful method for studying the spatial distribution of molecules. Preparation of tissue sections is a critical step for obtaining high-quality imaging data. The thickness of the slice of tissue affects the feature quality of MALDI MSI. However, few studies involved in-depth and systematic examination of slice thickness. Herein, we investigate the effect of tissue slice thickness on MALDI MSI detection. We found that the thicker the slice, the worse the results obtained by MALDI MS, which we attributed to the charging effect. The optimal slice thickness of brain tissue obtained in this work is 2-6 µm. Comparisons of the effects of slice thickness on atmospheric pressure and vacuum MALDI assays indicated that the ion signals and imaging quality of vacuum MALDI were more seriously affected by the thickness, with atmospheric pressure (AP) MALDI having a greater tolerance for slice thickness than vacuum MALDI. The MALDI MSI of peptides after enzymatic digestion of tissue sections of different thicknesses was also studied, revealing that the most suitable tissue thickness for enzyme digestion is about 10 µm. Finally, we optimized the slice thicknesses of six tissues in mice to provide a reference for MALDI MSI studies. It is worth mentioning that in our study the values of slice thickness range from the nanometer level (400 nm) at the minimum to 150 µm at the maximum, values which were unprecedented. Detailed in-depth and systematic studies of slice thickness will promote the development of sample preparation technology of AP and vacuum MALDI MSI, which will provide important references for the selection of tissue section thickness.
Assuntos
Peptídeos , Proteínas , Camundongos , Animais , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Peptídeos/análise , Diagnóstico por Imagem , EncéfaloRESUMO
A fluorinated covalent organic framework composed of 2,3,5,6-tetrafluoroterephthaldehyde (TFA) and 1,3,5-tri(4-aminophenyl)benzene (TAPB) is proposed for electrochromatographic separation. TFA-TAPB is for the first time regarded as the stationary phase of capillary electrochromatography. The TFA-TAPB-coated capillary columns exhibited satisfactory separation (resolution values > 1.5) and good reproducibility towards fluoroquinolones. The intraday relative standard deviations (RSDs) of retention time and peak areas were 0.54-0.68% and 1.69-2.82%, respectively. The interday RSDs of retention time and peak areas were less than 1.79% and 2.30%, respectively. The column-to-column RSDs of retention time and peak areas were 0.22-0.73% and 0.74-1.86%, respectively. And, the inter-batch RSDs of retention time and peak areas were less than 0.39% and 1.67%, respectively. Moreover, the possible separation mechanism was discussed, and it was found that the π-π stacking effect, hydrophobic interaction, hydrogen bonding, and fluorous interactions were the main factors. Overall, these results demonstrated that TFA-TAPB has high prospect for CEC separation.
Assuntos
Eletrocromatografia Capilar , Estruturas Metalorgânicas , Eletrocromatografia Capilar/métodos , Fluoroquinolonas , Interações Hidrofóbicas e Hidrofílicas , Estruturas Metalorgânicas/química , Reprodutibilidade dos TestesRESUMO
Molybdenum disulfide-graphene oxide composite (MoS2/GO) was synthesized and used as the adsorbent in dispersive solid-phase extraction. Four paraben preservatives, namely, methylparaben, ethylparaben, propylparaben, and butylparaben, were enriched with MoS2/GO and determined by ultra-high-performance liquid chromatography. Molybdenum disulfide was intercalated into graphene oxide layers to reduce self-aggregation by using the solvothermal method. The experimental results indicated that the as-prepared MoS2/GO composite exhibited great enrichment capability toward those four paraben preservatives, and the adsorption time was 10 min and the elution time was as short as 1 min. The mechanism of MoS2/GO composite and parabens is attributed to hydrogen bonding and electrostatic attraction. The relative standard deviation (RSD, n = 9) of this method was below 7.6%. Limits of detection and limits of quantification were in the range 0.4-2.3 ng/mL and 1.4-7.6 ng/mL, respectively. The recoveries obtained from the parabens of cosmetic sample were in the range 91.3-124% with RSDs below 10%. The developed method has great potential for the determination of emerging contaminants with low cost and high sensitivity.
RESUMO
A covalent organic framework (COF) named TpPa-1 was designed and synthesized at ambient temperature by an ultrasound-assisted method from 1,3,5-triformylphloroglucinol (Tp) and 1,4-phenylenediamine (Pa-1). It was utilized as a stationary phase in open-tubular capillary electrochromatography (OT-CEC). The column was coated with TpPa-1 using a covalent bonding strategy. The coated capillary was characterized by morphology, crystallography, and mesoporous analysis to confirm the successful fabrication. The OT-CEC method was utilized for the analysis of tetracyclines, sulfonamides, cephalosporins and amino acids with high-resolution (Rs > 1.81) and good precision (RSD < 4.9%). It takes about 12 h from COF preparation to OT-CEC separation. Graphical abstract A covalent organic framework (COF) named TpPa-1 was synthesized at ambient temperature by an ultrasound-assisted method from 1,3,5-triformylphloroglucinol (Tp) and 1,4-phenylenediamine (Pa-1). COF-TpPa-1 modified capillary column was utilized for the analysis of tetracyclines, sulfonamides, cephalosporins and amino acids with high-resolution and good precision.
Assuntos
Aminoácidos/análise , Eletrocromatografia Capilar , Preparações Farmacêuticas/análise , Fenilenodiaminas/química , Floroglucinol/análogos & derivados , Temperatura , Tamanho da Partícula , Floroglucinol/química , Propriedades de SuperfícieRESUMO
The interaction between the food colorant canthaxanthin (CA) and human serum albumin (HSA) in aqueous solution was explored by using fluorescence spectroscopy, three-dimensional fluorescence spectra, synchronous fluorescence spectra, UV-vis absorbance spectroscopy, circular dichroism (CD) spectra and molecular docking methods. The thermodynamic parameters calculated from fluorescence spectra data showed that CA could result in the HSA fluorescence quenching. From the KSV change with the temperature dependence, it was concluded that HSA fluorescence quenching triggered by CA is the static quenching and the number of binding sites is one. Furthermore, the secondary structure of HSA was changed with the addition of CA based on the results of synchronous fluorescence, three-dimensional fluorescence and CD spectra. Hydrogen bonds and van der Waals forces played key roles in the binding process of CA with HSA, which can be obtained from negative standard enthalpy (ΔH) and negative standard entropy (ΔS). Furthermore, the conclusions were certified by molecular docking studies and the binding mode was further analyzed with Discovery Studio. These conclusions can highlight the potential of the interaction mechanism of food additives and HSA.
Assuntos
Cantaxantina/química , Corantes de Alimentos/química , Albumina Sérica Humana/química , Sítios de Ligação , Humanos , Ligação de Hidrogênio , Simulação de Acoplamento Molecular , Estrutura Secundária de Proteína , Espectrometria de Fluorescência , TermodinâmicaRESUMO
Fluorescence spectroscopy and molecular simulation were explored to study the interaction between caffeic acid and human serum albumin (HSA). The experimental results indicated that the fluorescence quenching mechanism between caffeic acid and HSA is a static quenching, which was proved again by the analysis of fluorescence lifetime by time-correlated single photon counting. The binding process is spontaneous and the hydrophobic force is the main force between caffeic acid and HSA. In addition, the binding of caffeic acid to HSA was modeled by molecular dynamics simulations. The root mean square deviations, root mean square fluctuations, radius of gyration and the number of hydrogen bonds of the molecular dynamic (MD) simulation process were analyzed. Both experimental and modeling results demonstrated strong binding between HSA and caffeic acid. HSA had a slight conformational change when it binds with caffeic acid. The obtained information is useful for HSA drug design. Copyright © 2016 John Wiley & Sons, Ltd.
Assuntos
Ácidos Cafeicos/metabolismo , Simulação de Dinâmica Molecular , Albumina Sérica/metabolismo , Espectrometria de Fluorescência , Sítios de Ligação , Ácidos Cafeicos/química , Humanos , Ligação de Hidrogênio , Albumina Sérica/química , TermodinâmicaRESUMO
Endothelin-1 receptors (ETAR and ETBR) act as a pivotal regulator in the biological effects of ET-1 and represent a potential drug target for the treatment of multiple cardiovascular diseases. The purpose of the study is to discover dual ETA/ETB receptor antagonists from traditional Chinese herbs. Ligand- and structure-based virtual screening was performed to screen an in-house database of traditional Chinese herbs, followed by a series of in vitro bioassay evaluation. Aristolochic acid A (AAA) was first confirmed to be a dual ETA/ETB receptor antagonist based intracellular calcium influx assay and impedance-based assay. Dose-response curves showed that AAA can block both ETAR and ETBR with IC50 of 7.91 and 7.40 µM, respectively. Target specificity and cytotoxicity bioassay proved that AAA is a selective dual ETA/ETB receptor antagonist and has no significant cytotoxicity on HEK293/ETAR and HEK293/ETBR cells within 24 h. It is a feasible and effective approach to discover bioactive compounds from traditional Chinese herbs using in silico screening combined with in vitro bioassay evaluation. The structural characteristic of AAA for its activity was especially interpreted, which could provide valuable reference for the further structural modification of AAA.
Assuntos
Ácidos Aristolóquicos/farmacologia , Antagonistas do Receptor de Endotelina A/farmacologia , Antagonistas do Receptor de Endotelina B/farmacologia , Plantas Medicinais/química , Receptor de Endotelina A/efeitos dos fármacos , Receptor de Endotelina B/efeitos dos fármacos , Simulação por Computador , Avaliação Pré-Clínica de Medicamentos , Células HEK293 , HumanosRESUMO
The development of terahertz technology is attracting broad intention in recent years. The quality identification is important for the quality control of Chinese medicine production. In the present work, terahertz time-domain spectroscopy (THz-TDS) combined with partial least squares (PLS) were used for the identification model building and studied based on 41 official and unofficial rhubarb samples. First, the THz-TDS spectra of rhubarb samples were collected and were preprocessed by using chemometrics methods rather than transformed to absorption spectra. The identification models were then established based on the processed terahertz time domain spectra. The spectral preprocessing methods include Savitzky-Golay (S-G) first derivative, detrending, standard normal transformation (SNV), autoscaling, and mean centering. The identification accuracy of 90% was accomplished by using proper pretreatment methods, which was higher than the classified accuracy of 80% without any preprocessing for the time domain spectra. The component number of the PLS model was evaluated by leave-one-out cross-validation (LOOCV). The minimum values of the root-mean squared error of cross-validation (RMSECV) and root-mean squared error of prediction (RMSEP) were 0.076 6 and 0.169 0 by using mean centering method, respectively. The results of this work showed that the combination of terahertz time domain spectroscopy technology with chemometrics methods, as well as PLS can be applied for the recognition of genuine and counterfeit Chinese herbal medicines, as well as official and unofficial rhubarbs. The advantage of using terahertz time domain spectra directly with no transformation into absorption spectra is: (1) the thickness of samples could not be considered in the model establishment, and (2) the spectral processing was simplified. The proposed method based on the combination of THz-TDS and chemometrics proved to be rapid, simple, non-pollution and solvent free, suitable to be developed as a promising tool for quality control of many other Chinese herbal medicines.
Assuntos
Medicamentos de Ervas Chinesas/química , Rheum/química , Análise Espectral , Plantas Medicinais/química , Controle de QualidadeRESUMO
Determination of carbaryl in rice by using Fourier transform far-infrared (FT- Far-IR) and terahertz time-domain spectroscopy (THz-TDS) combined with chemometrics was studied and the spectral characteristics of carbaryl in terahertz region was investigated. Samples were prepared by mixing carbaryl at different amounts with rice powder, and then a 13 mm diameter, and about 1 mm thick pellet with polyethylene (PE) as matrix was compressed under the pressure of 5-7 tons. Terahertz time domain spectra of the pellets were measured at 0.5~1.5 THz, and the absorption spectra at 1.6. 3 THz were acquired with Fourier transform far-IR spectroscopy. The method of sample preparation is so simple that it does not need separation and enrichment. The absorption peaks in the frequency range of 1.8-6.3 THz have been found at 3.2 and 5.2 THz by Far-IR. There are several weak absorption peaks in the range of 0.5-1.5 THz by THz-TDS. These two kinds of characteristic absorption spectra were randomly divided into calibration set and prediction set by leave-N-out cross-validation, respectively. Finally, the partial least squares regression (PLSR) method was used to establish two quantitative analysis models. The root mean square error (RMSECV), the root mean square errors of prediction (RMSEP) and the correlation coefficient of the prediction are used as a basis for the model of performance evaluation. For the R,, a higher value is better; for the RMSEC and RMSEP, lower is better. The obtained results demonstrated that the predictive accuracy of. the two models with PLSR method were satisfactory. For the FT-Far-IR model, the correlation between actual and predicted values of prediction samples (Rv) was 0.99. The root mean square error of prediction set (RMSEP) was 0.008 6, and for calibration set (RMSECV) was 0.007 7. For the THz-TDS model, R. was 0. 98, RMSEP was 0.004 4, and RMSECV was 0.002 5. Results proved that the technology of FT-Far-IR and THz- TDS can be a feasible tool for quantitative determination of carbaryl in rice. This paper provides a new method for the quantitative determination pesticide in other grain samples.
Assuntos
Carbaril/análise , Oryza/química , Espectroscopia de Infravermelho com Transformada de Fourier , Espectroscopia Terahertz , Calibragem , Análise dos Mínimos QuadradosRESUMO
The metabotropic glutamate subtype 1 (mGluR1), a member of the metabotropic glutamate receptors, is a therapeutic target for neurological disorders. However, due to the lower subtype selectivity of mGluR1 orthosteric compounds, a new targeted strategy, known as allosteric modulators research, is needed for the treatment of mGluR1-related diseases. Recently, the structure of the seven-transmembrane domain (7TMD) of mGluR1 has been solved, which reveals the binding site of allosteric modulators and provides an opportunity for future subtype-selectivity drug design. In this study, a series of computer-aided drug design methods were utilized to discover potential mGluR1 negative allosteric modulators (NAMs). Pharmacophore models were constructed based on three different structure types of mGluR1 NAMs. After validation using the built-in parameters and test set, the optimal pharmacophore model of each structure type was selected and utilized as a query to screen the Traditional Chinese Medicine Database (TCMD). Then, three different hit lists of compounds were obtained. Molecular docking was used based on the latest crystal structure of mGluR1-7TMD to further filter these hits. As a compound with high QFIT and LibDock Score was preferred, a total of 30 compounds were retained. MD simulation was utilized to confirm the stability of potential compounds binding. From the computational results, thesinine-4'-O-ß-d-glucoside, nigrolineaxanthone-P and nodakenin might exhibit negative allosteric moderating effects on mGluR1. This paper indicates the applicability of molecular simulation technologies for discovering potential natural mGluR1 NAMs from Chinese herbs.
Assuntos
Cumarínicos/química , Medicamentos de Ervas Chinesas/química , Glucosídeos/química , Monossacarídeos/química , Alcaloides de Pirrolizidina/química , Receptores de Glutamato Metabotrópico/antagonistas & inibidores , Xantonas/química , Regulação Alostérica , Sítio Alostérico , Descoberta de Drogas , Ensaios de Triagem em Larga Escala , Humanos , Ligantes , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Ligação Proteica , Estrutura Terciária de Proteína , Receptores de Glutamato Metabotrópico/química , Interface Usuário-ComputadorRESUMO
Enrofloxacin (ENR) residues in animal-derived food and water threaten human health. Simple, low-cost and on-site detection methods are urgently needed. Blue emitting carbon quantum dots (CQDs) and orange rhodamine B (RhB) were used as recognition and reference signals, respectively, to construct a ratiometric fluorescence sensor. After the addition of ENR, the color of the sensor changed from orange to blue because hydrogen bonding induced a considerable increase in CQDs fluorescence. Based on this mechanism, a simple and low cost on-site portable sensing platform was constructed, which integrated a stable UV light strip and a smartphone with voice-controlled phototaking function and an RGB app. The t-test results of spiked ENR recoveries for diluted milk, honey and drinking water revealed no significant differences between the ratiometric fluorescent sensor and portable sensing platform. Thus, this portable sensing platform provides a novel strategy for on-site quantification of quinolone antibiotics in foodstuffs and environmental water.
Assuntos
Antibacterianos , Enrofloxacina , Contaminação de Alimentos , Ligação de Hidrogênio , Leite , Pontos Quânticos , Smartphone , Enrofloxacina/análise , Pontos Quânticos/química , Leite/química , Contaminação de Alimentos/análise , Antibacterianos/análise , Animais , Fluorescência , Poluentes Químicos da Água/análise , Mel/análise , Espectrometria de Fluorescência/instrumentação , Espectrometria de Fluorescência/métodos , Água Potável/análise , Carbono/química , Rodaminas/químicaRESUMO
A two-step modeling approach was employed to study the selectivity and activity of histone deacetylase inhibitors. First, according to the activity difference against HDAC1 and HDAC6, a binary classification model was established to classify two kinds of inhibitors. Then two continuous models were built for each subclass to predict the activity value of HDAC1 and HDAC6 inhibitors. The three models were all built with the GA-kNN method combined with dragon descriptors. They were external validated by using external prediction set and Y-randomization test. The highly predictive models were generated for all three data sets. For the classification model, the classification accuracies of the models were as high as 100% for the external test set. For HDAC1 and HDAC6 inhibitor consecutive models, external R(2) values are 0.947 and 0.911, respectively. The results proved the reliability of these models. All models were used to screen 1000 compounds included in PubMed dataset. Virtual screening resulted in 8 and 13 structurally unique consensus hits that were considered novel putative HDAC1 and HDAC6 inhibitors, respectively.
Assuntos
Inibidores de Histona Desacetilases/química , Inibidores de Histona Desacetilases/farmacologia , Modelos Químicos , Humanos , Modelos Moleculares , Relação Quantitativa Estrutura-Atividade , Fluxo de TrabalhoRESUMO
Terahertz science and technology is increasingly emphasized in science and industry, and has progressed significantly in recent years. There is an important aspect of attention in the application of terahertz technology to medicine. The overview of the terahertz characters, terahertz spectroscopy and terahertz imaging technology is introduced. This paper focuses on reviewing the use of and research progress in terahertz spectroscopy and terahertz imaging technology in medical testing and diagnosis. Furthermore, the problems to be solved and development directions of terahertz spectroscopy and terahertz imaging technology are discussed.
Assuntos
Diagnóstico por Imagem/tendências , Testes Diagnósticos de Rotina/métodos , Imagem Terahertz/tendências , Espectroscopia Terahertz/tendências , Testes Diagnósticos de Rotina/tendências , Humanos , Laboratórios Hospitalares , Imagem Terahertz/métodos , Radiação Terahertz , Espectroscopia Terahertz/métodosRESUMO
Endometrial carcinoma is one of the most common gynecologic cancers. The present paper reports a new application of Logistic regression to building model of endometrial cancer. Near infrared (NIR) spectra was introduced. In our study, the NIR spectra of 77 specimens were pretreated by principal component-linear discriminant analysis (PC-LDA) and support vector machine discriminant analysis (SVM-DA). Latin partition method for selecting training and test sets was used to determine the significant parameters for Logistic regression model. From the predicted results of logistic regression model, both the categories of samples and the trends of samples belonging to other class were clear and concordant with the clinical result. The proposed procedure proved to be suitable to being developed as a noninvasive diagnosis method for cancer tissue.
Assuntos
Neoplasias do Endométrio/diagnóstico , Neoplasias do Endométrio/patologia , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Endométrio/patologia , Feminino , Humanos , Modelos Logísticos , Análise de Componente Principal , Máquina de Vetores de SuporteRESUMO
Tissue factor pathway inhibitor (TFPI) is an endogenous inhibitor of the extrinsic coagulation pathway. In patients with hemophilia A or B, inhibition of TFPI is an alternative therapeutic approach that augments the extrinsic coagulation pathway. Marstacimab is an investigational fully human monoclonal antibody that binds and neutralizes TFPI and is being evaluated as a prophylactic treatment to prevent or reduce the frequency of bleeding episodes in patients with severe hemophilia A or B, with or without inhibitors (antibodies against coagulation factors). However, the efficacy, safety, and pharmacokinetics of marstacimab may be affected by the induction of antidrug antibody (ADA) responses. Here, we describe the evolution and validation of three quasi-quantitative electrochemiluminescence-based methods to detect marstacimab ADAs, starting from their use in a first-in-human phase 1 study to their use in phase 2 and 3 clinical studies of patients with severe hemophilia. For all three methods, validation criteria evaluated the performance of the assays in screening and confirmatory cut points, precision, selectivity, drug tolerance, target interference, and stability. Additional criteria for validation were dilution linearity (Methods 1 and 2) and low positive control concentration, prozone effect, plate homogeneity, and robustness (Method 3). The three methods met validation criteria and are a potentially valuable tool in detecting the induction of marstacimab ADAs during treatment in patients with hemophilia.
Assuntos
Hemofilia A , Humanos , Hemofilia A/tratamento farmacológico , Anticorpos Monoclonais Humanizados , Anticorpos MonoclonaisRESUMO
In this work, a three-dimensional fluorinated covalent organic frameworks (3D FCOFs) JUC-515 was synthesized from tetra(4-aminophenyl)methane (TAM) and 2,3,5,6-tetrafluoroterephthalol (TFA) by an ionic liquid method. JUC-515 was introduced into the capillary column and bonded to the inner wall of the capillary column by chemical bonding. Through a variety of characterization results, JUC-515 was successfully synthesized and introduced into the capillary column. The effects of buffer solution concentration, organic additive content and pH of the buffer solution on the separation of fluoroquinolones (FQs) were investigated in detail. The JUC-515-coated capillary column showed good resolution (>1.5) and reproducibility. The relative standard deviations (RSDs) of the retention time for intraday, interday, column-to-column and interbatch precision were less than 0.88%, 2.45%, 2.74% and 3.32%, respectively. The RSDs of the peak area for intraday, interday, column-to-column and interbatch precision were less than 3.79%, 4.31%, 3.33% and 5.62%, respectively. The JUC-515-coated capillary column could be used no less than 150 times. The results showed that the JUC-515-coated capillary column had good separation performance. In addition, by separating fluorinated ß-phenylalanine analogs, ß-phenylalanine and trifluoromethyl ß-phenylalanine analogs, the separation mechanism based on fluorine interactions was discussed. In conclusion, JUC-515 had good potential as a stationary phase for capillary electrochromatography.
Assuntos
Eletrocromatografia Capilar , Estruturas Metalorgânicas , Estruturas Metalorgânicas/química , Eletrocromatografia Capilar/métodos , Fluoroquinolonas , Reprodutibilidade dos Testes , FenilalaninaRESUMO
In the present article, near infrared spectra of 89 wood samples of different geographical provenances and species were measured, and back propagation artificial neural networks(BPANN) and generalized regression neural network (GRNN) were used for modeling of wood species NIRS identifying. Parameters for two neural networks were chosen via analysis of variance, respectively; and networks were trained with optimum parameters. Considering the difference between spectra, spectra with different levels of white noise and different levels of bias were simulated and predicted by using the models built. It was found that both the two models had satisfactory prediction results, identification correct rates obtained by BPANN model applied to spectra with bias level no higher than 2% and noise level no higher than 4% were above 97%; correct rates obtained by GRNN model applied to spectra with bias level no higher than 2% and noise level no higher than 4% were above 99%.
Assuntos
Espectroscopia de Luz Próxima ao Infravermelho , Madeira/classificação , Modelos Teóricos , Redes Neurais de ComputaçãoRESUMO
In this work, four kinds of covalent organic framework (COF) materials (TpPa-1, TpBD, TpDT, and TFBBD) with different pore sizes or functional groups were synthesized by an ultrasonic method for the adsorption of five sulfonamides. Optimization experiments regarding the adsorption time, vortex speed, and pH were carried out to improve adsorption efficiency. In addition, kinetic and thermodynamic experiments were conducted to explore the adsorption mechanism of the sulfonamides on the different COFs. The adsorption processes of the five sulfonamides on the four COFs fit the pseudo-second-order kinetic model and Langmuir adsorption isotherm model. Additionally, pore filling, hydrogen bond interactions, and electrostatic attraction were found to be the main adsorption mechanisms.
Assuntos
Estruturas Metalorgânicas , Adsorção , Cinética , Estruturas Metalorgânicas/química , Sulfanilamida , SulfonamidasRESUMO
Near-infrared spectroscopy combined with chemometrics methods for diagnosis of cancer has been reported in literatures. In our study, the NIR spectra of 77 specimens of different physiological stages of endometrium were collected. Spectral data were pretreated firstly by multiplicative scatter correction (MSC), orthogonal signal correction (OSC), and both of them, respectively, and then by SG smoothing. Latin partition method was used to select 3/4 samples as a training set, and the other 1/4 samples for test set. Support vector machine (SVM) model was built for classification, and the classification results was compared with that of partial least squares (PLS) model based on the same pretreatment methods. Samples of malignant, hyperplasia and normal endometrium were classified better by SVM (classification accuracy was 92%) than PLS (classification accuracy was 90%). The results suggested that classification accuracy was affected by pretreatment methods and models. SVM combined with endometrial tissue near infrared spectroscopy is expected to develop into a new approach to tumor diagnosis.
Assuntos
Detecção Precoce de Câncer/métodos , Neoplasias do Endométrio/diagnóstico , Espectroscopia de Luz Próxima ao Infravermelho , Máquina de Vetores de Suporte , Endométrio/patologia , Feminino , Humanos , Análise dos Mínimos Quadrados , Modelos TeóricosRESUMO
The Schiff-base reaction of 1,3,5-triformylphloroglucinol (Tp) and tetra(4-aminophenyl)methane (TAM) was performed for the synthesis of a three-dimensional covalent organic framework named 3D TpTAM, which was obtained by an ultrasound-assisted method for the first time. The morphology and structure of the synthesized TpTAM were characterized through various methods. Then, TpTAM-coated capillary columns were subsequently prepared by a covalent bonding method within a short time and applied for the separation of fluoroquinolones by capillary electrochromatography (CEC) with good resolution and reproducibility. The intraday relative standard deviations (RSDs) of the retention time and peak areas were 0.88%-0.95% and 2.27%-3.81%, respectively. The interday RSDs of retention time and peak areas were 0.71%-0.89% and 0.88%-3.60%, respectively. The column-to-column RSDs of retention time and peak areas were less than 1.90% and 13.56%, respectively. The interbatch RSDs of retention time and peak areas were less than 3.48% and 3.89%, respectively. The TpTAM-coated capillary columns could be used for no less than 100 runs with no observable changes in the separation efficiency. The separation mechanism was also studied, which indicated that π-π stacking effects, hydrophobic interactions and hydrogen bonding were the main factors. The results revealed that 3D TpTAM should have superior potential as the stationary phase in CEC for chromatographic separation.