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1.
Environ Sci Technol ; 58(17): 7617-7627, 2024 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-38632682

RESUMO

Commercial chemicals, such as synthetic musks, are of global concern, but data on their occurrence and spatial distribution in aquatic environments of large scale are scarce. Two sampling campaigns were conducted in the present study to measure freely dissolved synthetic musks in freshwaters across China using passive samplers, along with biological coexposure at selected sites. Polycyclic musks (PCMs) dominated synthetic musks, with a detection frequency of 95%. Higher concentrations of PCMs were observed in densely populated Mid, East, and South China compared to less populated regions, indicating the significance of anthropogenic activities for synthetic musks in water. The concentration ratios of galaxolide (HHCB)/tonalide (AHTN) were significantly higher in low-latitude areas than in high-latitude areas from June to September, suggesting that solar radiation played an important role in the degradation of HHCB/AHTN. Significant correlations were found between dissolved concentrations of HHCB and AHTN and their lipid-normalized concentrations in coexposed fish and clam. The estimated hazard quotients for HHCB and AHTN in freshwater fish consumed by humans were less than 0.01 at all sampling sites except the Yangtze River Basin. These results help to understand the environmental fate and ecological risks of synthetic musks on a large geographical scale.


Assuntos
Água Doce , Poluentes Químicos da Água , China , Poluentes Químicos da Água/análise , Água Doce/química , Monitoramento Ambiental , Bioacumulação , Benzopiranos , Animais , Tetra-Hidronaftalenos/análise , Peixes/metabolismo , Ácidos Graxos Monoinsaturados
2.
Nano Lett ; 23(18): 8816-8826, 2023 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-37459451

RESUMO

Sarcopenia is known as age-related muscle atrophy, which influences over a quarter of the elderly population worldwide. It is characterized by a progressive decline in muscle mass, strength, and performance. To date, clinical treatments in sarcopenia are limited to rehabilitative interventions and dietary supplements. Tetrahedral framework nucleic acids (tFNAs) represent a novel kind of DNA-based nanomaterial with superior antiapoptosis capacity in cells, tissues, organs, and systems. In our study, the therapeutic effect of tFNAs treatment on sarcopenia was evaluated both in vivo and in vitro. Results from muscular biophysiological characteristics demonstrated significant improvement in muscle function and endurance in the aged mouse model, and histologic examinations also showed beneficial morphological changes in muscle fibers. In vitro, DEX-induced sarcopenic myotube atrophy was also ameliorated through the inhibition of mitochondria-mediated cell apoptosis. Collectively, tFNAs treatment might serve as an alternative option to deal with sarcopenia in the near future.


Assuntos
Ácidos Nucleicos , Sarcopenia , Humanos , Idoso , Camundongos , Animais , Sarcopenia/tratamento farmacológico , Sarcopenia/patologia , Ácidos Nucleicos/uso terapêutico , Atrofia Muscular/tratamento farmacológico , Atrofia Muscular/patologia , Apoptose , Mitocôndrias/patologia
3.
Osteoporos Int ; 34(12): 2087-2100, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37648795

RESUMO

We used data from the NHANES to explore associations of DOBS with femur BMD and osteoporosis in postmenopausal women. We found that DOBS was positively associated with femur BMD and negatively associated with the risk of osteoporosis in postmenopausal women. PURPOSE: The study aimed to investigate the relationship between dietary oxidative balance score (DOBS) and the risk of osteoporosis in American postmenopausal women. METHODS: A total of 3043 participants were included in this study. The linear relationship between DOBS and femur BMD was evaluated using a weighted multivariate linear regression model. The association between DOBS and the risk of osteoporosis was assessed using a weighted logistic regression model, with odds ratios (ORs) and 95% confidence intervals (CIs) calculated. Moreover, the relationship was further characterized through smooth curve fitting (SCF) and weighted generalized additive model (GAM) analysis. RESULTS: After adjusting for all covariates, the weighted multivariable linear regression models showed a positive correlation between DOBS and femur BMD. Moreover, the weighted logistic regression model demonstrated that compared to the first tertile of DOBS, the highest tertile of DOBS was significantly associated with a lower risk of osteoporosis, with ORs of 0.418 (95% CI, 0.334, 0.522) for individuals under the age of 70 and 0.632 (95% CI, 0.506, 0.790) for individuals aged 70 or above. Similar trends were also observed in SCF and GAM models. CONCLUSION: The present study indicated that postmenopausal women with a higher DOBS have a lower risk of femur osteoporosis. This finding may highlight the potential protective role of an antioxidant-rich diet for the bones of the postmenopausal population. Moreover, DOBS may also be a valuable tool in identifying individuals with osteoporosis. Screening and early intervention for osteoporosis may be essential for postmenopausal women with low DOBS.


Assuntos
Osteoporose Pós-Menopausa , Osteoporose , Humanos , Feminino , Inquéritos Nutricionais , Densidade Óssea , Pós-Menopausa , Osteoporose/epidemiologia , Fêmur , Dieta , Estresse Oxidativo , Osteoporose Pós-Menopausa/epidemiologia
4.
BMC Musculoskelet Disord ; 24(1): 311, 2023 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-37081472

RESUMO

BACKGROUND: Femoral head fractures are rare injuries often associated with poor functional outcomes and complications. The purpose of this study was to evaluate the incidence, treatment methods and approaches, complications, and functional outcomes of femoral head fractures. METHODS: We retrospectively reviewed 50 patients who sustained femoral head fractures between January 2011 and December 2018. There were thirty-seven (74%) males and thirteen (26%) females with a median age of 40 years. According to Pipkin's classification, there were eighteen (36%) Pipkin I, ten (20%) Pipkin II, eight (16%) Pipkin III, and fourteen (28%) Pipkin IV patients. Treatment methods were categorized into non-operative, operative by open reduction and internal fixation (ORIF), and immediate total hip replacement (THR). The recorded surgical approach consists of an anterior(S-P) approach, posterior(K-L) approach, lateral stab, and combined anterior + lateral stab approach for fixation. The patients were also stratified by the Injury Severity Score (ISS), associated injuries, and, mechanism of injuries. The modified harris hip score (MHHS) was used to evaluate the ongoing complications with the clinical outcome of patients with two years or greater follow-up. RESULTS: Eight (16%) patients were managed successfully with closed reduction without surgery and thirty-seven (74%) patients required operative reduction and internal fixation (ORIF) of the femoral head and acetabulum, and 5 (10%) patients required immediate THR. Six (12%) patients developed AVN, and four (8%) required a secondary THR. Sixteen patients (33%) developed post-traumatic osteoarthritis (PTOA), eight (16%) developed heterotopic ossification (HO) and six patients (12%) had sciatic nerve injury, none requiring operative treatment. Overall functional results according to MHHS were, excellent in two (4%) patients, good in sixteen (32%) patients, fair in twenty-two (44%) patients, and poor in ten (20%) patients. A statistically significant difference in outcome was observed among four pipkin subtypes. CONCLUSION: Femoral head fractures are rare injuries often associated with poor outcomes. In this study, we report the functional outcomes and complications of all treatment approaches for femoral head fracture based on the Pipkin classification. The treatment aim should always be the anatomical reduction of the fragments. This study, adds to the growing literature on femoral head fracture and provides a reference for the clinical treatment to guide patient management. TRIAL REGISTRATION: Our study was approved by the Clinical Research and Biomedical Ethical Committee of West China Hospital, Sichuan University, and was performed in accordance with the Declaration of Helsinki. All participants provided written informed consent to participate in this study.


Assuntos
Fraturas do Fêmur , Fraturas do Quadril , Masculino , Feminino , Humanos , Adulto , Estudos Retrospectivos , Seguimentos , Cabeça do Fêmur/diagnóstico por imagem , Cabeça do Fêmur/cirurgia , Cabeça do Fêmur/lesões , Prognóstico , Fraturas do Fêmur/cirurgia , Fixação Interna de Fraturas/efeitos adversos , Fixação Interna de Fraturas/métodos , Resultado do Tratamento , Fraturas do Quadril/cirurgia
5.
BMC Musculoskelet Disord ; 24(1): 285, 2023 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-37055749

RESUMO

BACKGROUND: Recently, some studies on the efficacy of the femoral neck system (FNS) in treating femoral neck fractures (FNFs) have been published. Therefore, a systematic review was performed to clarify the efficacy and safety of FNS versus cannulated screws (CS) for the treatment of FNFs. METHOD: The PubMed, EMBASE, and Cochrane databases were systematically searched for studies comparing FNS and CS fixations in FNFs. Intraoperative indicators, postoperative clinical indicators, postoperative complications, and postoperative scores were compared between the implants. RESULTS: A total of eight studies were included in the study, involving 448 FNFs patients. The results showed that patients in FNS group were significantly lower than the CS group in the number of X-ray exposures (WMD = -10.16; 95% CI, -11.44 to -8.88; P < 0.001; I2 = 0%), fracture healing time (WMD = -1.54; 95% CI, -2.38 to -0.70; P < 0.001; I2 = 92%), length of femoral neck shortening (WMD = -2.01; 95% CI, -3.11 to -0.91; P < 0.001; I2 = 0%), femoral head necrosis (OR = 0.27; 95% CI, 0.08 to 0.83; P = 0.02; I2 = 0%), implant failure/cutout (OR = 0.28; 95% CI, 0.10 to 0.82; P = 0.02; I2 = 0%), and Visual Analog Scale Score (WMD = -1.27; 95% CI, -2.51 to -0.04; P = 0.04; I2 = 91%). And the Harris Score was significantly higher in the FNS group than in the CS group (WMD = 4.15; 95% CI, 1.00 to 7.30; P = 0.01; I2 = 89%). CONCLUSIONS: Based on this meta-analysis, FNS shows better clinical efficacy and safety in treating FNFs compared to CS. However, due to the limited quality and number of included studies and the high heterogeneity of the meta-analysis; large samples and multicenter RCTs are needed to confirm this conclusion in the future. LEVEL OF EVIDENCE: II, Systematic review and Meta-analysis. TRIAL REGISTRATION: PROSPERO CRD42021283646.


Assuntos
Fraturas do Colo Femoral , Colo do Fêmur , Humanos , Colo do Fêmur/cirurgia , Fixação Interna de Fraturas/efeitos adversos , Fixação Interna de Fraturas/métodos , Parafusos Ósseos , Fraturas do Colo Femoral/cirurgia , Resultado do Tratamento , Estudos Retrospectivos , Estudos Multicêntricos como Assunto
6.
J Environ Manage ; 344: 118478, 2023 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-37393876

RESUMO

The implementation of Personal Carbon Trading (PCT) holds promise in facilitating a noteworthy contribution towards the attainment of emissions reduction predicated on consumption patterns and consequently motivating lifestyle modifications. As individual consumption behaviors usually lead to continuous changes in carbon emissions, it is crucial to rethink PCT from a systematic perspective. This review employed a bibliometric analysis of 1423 papers related to PCT, highlighting the key themes of carbon emissions from energy consumption, climate change, and public opinion on policies in the context of PCT. Most of the existing PCT researches focus on theoretical assumptions and public attitudes, while the quantification of carbon emissions and simulation of PCT require further investigation. Furthermore, the concept of Tan Pu Hui is seldom addressed in PCT studies and case analyses. Moreover, there are limited PCT schemes worldwide that can be directly implemented in practice, leading to a scarcity of large-scale, high-participation case studies. To address these gaps, this review proposes a framework to clarify how PCT can stimulate individual emission reductions on the consumption side, comprising two phases, from motivation to behavior and behavior to target. Future endeavors should prioritize the enhancement of the systematic study of the theoretical foundation of PCT, encompassing carbon emissions accounting and policy design, the incorporation of cutting-edge technology, and the reinforcement of integrated policy practice. This review serves as a valuable reference for future research endeavors and policymaking efforts.


Assuntos
Carbono , Formulação de Políticas , Mudança Climática , Simulação por Computador , Dióxido de Carbono/análise , China
7.
J Environ Manage ; 326(Pt B): 116828, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36436243

RESUMO

The aquatic environment, linked to the sustainable development of human existence and ecological environment, is influenced comprehensively by anthropogenic and natural activities. In light of the continuously low concentration of dissolved oxygen (DO) in surface water in plain river networks and the phenomenon of delay in the improvement of surface water quality, this research aims to introduce a method that may be utilized in identifying the critical driving forces of DO in surface water and their lagging characteristics, which will contribute to the assessment and adjustment of water quality drivers and/or policies. The research analyzes a typical small watershed in a river network region of the Yangtze River Delta plain as the study area, collecting 35-year (1986-2020) data on several water quality parameters, decades of anthropogenic activities, and two natural factors. The time series methods of vector autoregressive model, Granger causality tests, forecast error variance decompositions, and impulse response functions (hereinafter referred to as VAR+), which are rarely applied in related research, were employed in this study and proved helpful for screening out pivotal drivers and capturing the lagging responses of DO level to driving forces at each lagged time. Results show that there exists a fluctuating drop in DO level in surface water from 1986 to 2008 and a steady climb from 2008 to 2020, with the lowest DO level being present in 2008. The impulsive perturbations of phosphate fertilizer consumption (PFC), motor vessel number, and precipitation minimally increase DO concentration, while the impulsive perturbation of gross domestic product (GDP) causes the sharpest drop in DO level. With these perturbations, the driving force of PFC persists for approximately seven years, and the driving forces of water temperature, permanent population, and GDP persist for only five years. Future research could be conducted with spatial hysteresis, selection of lag order and variable quantity within the model, as well as intermediate variables between drivers and DO level for exploring driving pathways and mechanisms.


Assuntos
Monitoramento Ambiental , Oxigênio , Humanos , Efeitos Antropogênicos , Qualidade da Água , Rios , China
8.
Arch Orthop Trauma Surg ; 143(4): 1957-1963, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35254500

RESUMO

PURPOSE: A novel 3D reduction method combined with a modified Kirschner-wire tension band (3D MKTB) fixation method to treat comminuted patella fractures was introduced in this study. This study aims to evaluate the effectiveness and practicality of this novel technology. METHODS: This study is a retrospective case series study. Twenty-eight patients with closed comminuted patella fractures were treated with the novel 3D MKTB fixation method. Radiographs of the knee were obtained at the routine follow-up to assess fracture healing and widening of the articular step-off. Clinical outcomes including the degree of pain on a visual analogue scale (VAS), range of motion, Lysholm, and Bostman grading scales were measured at the last follow-up. The mean follow-up was 39.8 ± 10.5 (range 26-62) months. RESULTS: All patients had bony union at a mean of 11.4 ± 1.4 (range 9-14 weeks) weeks based on X-ray. No patient had evidence of internal fixation failure. Only two patients suffered from surgical complication. One patient suffered from postoperative superficial infection, and one patient suffered from internal fixation irritation. Articular step-off larger than 2 mm was not seen in any cases. The average VAS-related pain score was 0.5 ± 0.5 (range 0-1) points. The average range of motion was 0-138.6° ± 11.9° (range 90°-155°), all patients had full knee extension. The mean Lysholm and Bostman scores were 92.9 ± 3.5 (range 86-100 points) points and 28.5 ± 1.3 (range 24-30 points) points, respectively. CONCLUSION: The novel 3D reduction technique combined with the modified Kirschner-wire tension band technique is an effective, safe, and simple treatment option for comminuted patella fractures. LEVEL OF EVIDENCE: IV.


Assuntos
Fraturas Ósseas , Traumatismos do Joelho , Fratura da Patela , Humanos , Estudos Retrospectivos , Parafusos Ósseos , Patela/cirurgia , Fraturas Ósseas/cirurgia , Traumatismos do Joelho/cirurgia , Complicações Pós-Operatórias , Dor
9.
J Vasc Surg ; 75(6): 1918-1925, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-34974141

RESUMO

OBJECTIVE: In the present study, we evaluated and compared the outcomes of transarterial embolization with those of percutaneous contrast-enhanced ultrasound-guided transabdominal sac embolization (PUSE) for type II endoleaks (T2ELs). METHODS: A retrospective review was conducted of consecutive patients who had undergone T2EL embolization between January 2015 and December 2020 at our center. The cohort was divided into two groups according to the embolization approach: PUSE vs transarterial. Freedom from aneurysm growth, safety, immediate technical success, freedom from persistent T2ELs, and the repeat embolization rate were assessed. RESULTS: A total of 25 patients and 28 embolization procedures (PUSE, n = 16; transarterial embolization, n = 12) were examined. Both the fluoroscopic time (13.3 ± 3.2 minutes vs 35.0 ± 7.0 minutes; P < .001) and the procedural time (84.9 ± 8.4 minutes vs 117.1 ± 14.8 minutes; P < .001) were significantly shorter in the PUSE group than in the transarterial group. After the embolization procedure, the patients were followed up for a mean duration of 24.7 ± 14.9 months for the PUSE group and 35.9 ± 21.1 months for the transarterial group (P = .1323). Five patients in the transarterial group had undergone unsuccessful embolization, with success in 7 of the 12 patients in the transarterial group and all 16 patients in the PUSE group (P = .0081). Failure had resulted from failed transarterial access or a recurrent T2EL. Three of the five patients had undergone subsequent PUSE during follow-up. No patient in the PUSE group had experienced sac expansion compared with four patients in the transarterial group (P = .0242). Similarly, no patient in the PUSE group had developed a newly discovered T2EL vs four patients in the transarterial group (P = .0242). Thus, the outcomes were markedly better for the PUSE group than were those for the transarterial group. A major procedure-related complication (abdominal abscess) occurred in one patient in the transarterial group. CONCLUSIONS: PUSE is safe and effective for managing T2ELs. It yields better outcomes in terms of preventing aneurysm growth, decreasing the incidence of repeat embolization and complications, minimizing the recurrence of T2ELs, and reducing the fluoroscopic and procedural times. We, thus, regard it as the preferred approach for the management of T2ELs.


Assuntos
Aneurisma da Aorta Abdominal , Implante de Prótese Vascular , Embolização Terapêutica , Procedimentos Endovasculares , Aneurisma da Aorta Abdominal/complicações , Aneurisma da Aorta Abdominal/diagnóstico por imagem , Aneurisma da Aorta Abdominal/cirurgia , Implante de Prótese Vascular/efeitos adversos , Embolização Terapêutica/efeitos adversos , Embolização Terapêutica/métodos , Endoleak/diagnóstico por imagem , Endoleak/etiologia , Endoleak/terapia , Procedimentos Endovasculares/efeitos adversos , Humanos , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento , Ultrassonografia de Intervenção/efeitos adversos
10.
Neurol Sci ; 43(4): 2823-2830, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34373992

RESUMO

PURPOSE: Alcohol-induced osteonecrosis femoral head necrosis (ONFH) is a disease that seriously affects human health. Abnormal expression of L3MBTL3/PTPN9 gene can cause a variety of human diseases. The purpose of this study is to investigate the effect of L3MBTL3/PTPN9 gene polymorphism on the susceptibility of alcohol-induced ONFH in Chinese Han population. METHODS: A total of 308 alcohol-induced ONFH patients and 425 healthy controls were enrolled in this case-control study. Alleles, genotypes, genetic models, haplotypes, and multifactor dimensionality reduction analyses (MDR) based on age-corrected by using odds ratio (OR) and 95% confidence interval (CI) were performed. RESULTS: Our result revealed rs2068957 in the L3MBTL3 gene increased the risk of alcohol ONFH under the recessive model after correction. Besides, we also found that rs75393192 in the PTPN9 gene was a protective site in stratification over 40 years of age and stage. In stratified analysis of necrotic sites, we only found that rs2068957 was associated with increased susceptibility of alcohol-induced ONFH under the co-dominant model and recessive model. Haplotype "GC" in the block (rs76107647|rs10851882 in PTPN9 gene) significantly decreased the susceptibility of alcoholic ONFH. CONCLUSIONS: Our results provide evidence that L3MBTL3/PTPN9 polymorphisms are associated with alcohol-induced ONFH risk in Chinese Han population.


Assuntos
Povo Asiático , Etnicidade , Necrose da Cabeça do Fêmur , Predisposição Genética para Doença , Polimorfismo Genético , Adulto , Povo Asiático/genética , Estudos de Casos e Controles , China , Etnicidade/genética , Necrose da Cabeça do Fêmur/induzido quimicamente , Necrose da Cabeça do Fêmur/epidemiologia , Necrose da Cabeça do Fêmur/genética , Predisposição Genética para Doença/genética , Haplótipos , Humanos , Pessoa de Meia-Idade , Polimorfismo Genético/genética
11.
BMC Surg ; 22(1): 69, 2022 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-35219291

RESUMO

BACKGROUND: After severe trauma of lower limbs, bone, tendon or plate graft exposure is common. The traditional repair method is to use a variety of skin flap transplantation to cover the exposed part, but the wound often can not heal after operation, or the wound is cracked, ulcer, sinus, bone and steel plate are exposed again after wound healing. The reason for this result is that when the flap is covered, the space around the bone plate is not well closed, forming a dead cavity, blood and exudate accumulation, hematoma formation or infection, and finally the wound ruptures again. In addition, due to the swelling and contracture of the flap after operation, the suture tension between the flap and the receiving area becomes larger, the skin becomes thinner and broken, and then the wound is formed. In order to solve the above problems, we carried out the study of artificial true skin embedding combined with fascial sleeve flap transplantation in the treatment of chronic bone plate exposed wounds of lower limbs. METHODS: In this paper, 11 cases of chronic wounds with bone exposure and skin necrosis after steel plate implantation were selected. First stage is the wound bed preparation including primary wound expansion, removal of necrotic tissue and incision of sinus wall, removal of deep necrotic bone and fibrotic scarred skin on the outer wall of steel plate to normal tissue on the outer edge of the wound, removal of precipitated peptone and purulent fur in the hole, periphery and bone space of the steel plate, and removal of tendon tissue with basal necrosis and disintegration of the wound. After vacuum sealing drainage (VSD) 1-2 weeks, the peritraumatic basal granulation tissue grew well and there was no necrotic tissue in the wound. In the second stage, the exposed bone was covered with artificial dermis, the steel plate hole or the periphery and the basal space were filled, and the exposed steel plate was completely embedded, and then the fascia sleeve flap was transplanted to cover the wound. The sural neurovascular flap was performed in nine cases and the lateral superior malleolar artery perforator flap in two case. RESULTS: The flap survived well in all 11 cases. During the follow-up of 6 months to the removal of the plate, there was no case of rupture, exposure and sinus formation. CONCLUSIONS: Artificial dermal covering combined with fascial sleeve flap transplantation can effectively avoid wound dehiscence or sinus formation caused by foreign body retention, infection and flap contracture. It has good effect in repairing chronic wounds with bone plate exposure after severe trauma of lower limbs.


Assuntos
Retalho Perfurante , Procedimentos de Cirurgia Plástica , Lesões dos Tecidos Moles , Placas Ósseas , Humanos , Extremidade Inferior/cirurgia , Transplante de Pele/métodos , Lesões dos Tecidos Moles/cirurgia , Resultado do Tratamento
12.
Geriatr Nurs ; 44: 69-75, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35074539

RESUMO

OBJECTIVE: To assess the effectiveness of a preoperative mobilization program for preventing perioperative complications and improving function in older adults with femoral neck fracture who delayed surgery. METHODS: In this controlled, quasi-experimental study, a total of 150 older patients with femoral neck fracture who waited more than 2 days before surgery were assigned to the Intervention group or control group. Perioperative complications were recorded. The change of physical function was assessed using the modified Barthel index (MBI). RESULTS: The overall perioperative complication rate (52.2% vs. 73.5%), pulmonary infection (6.0% vs. 16.9%), delirium (9.0% vs. 24.1%) in the intervention group were lower than those in the control group. The intervention group had significantly better improvement in MBI score preoperatively and postoperatively. CONCLUSION: A preoperative mobilization program was shown to have advantages in preventing perioperative complications and promoting early functional recovery in older adults with delayed surgery of femoral neck fracture.


Assuntos
Fraturas do Colo Femoral , Pneumonia , Idoso , Fraturas do Colo Femoral/complicações , Fraturas do Colo Femoral/cirurgia , Humanos , Recuperação de Função Fisiológica , Estudos Retrospectivos , Resultado do Tratamento
13.
Zhonghua Nan Ke Xue ; 28(5): 387-394, 2022 May.
Artigo em Zh | MEDLINE | ID: mdl-37477476

RESUMO

Objective: To investigate the improving effect of human urine-derived stem cell-derived exosomes (USC-Exo) on the endothelial function and erectile function of male rats with diabetic ED (DED) and explore their action mechanism. METHODS: USC-Exo were extracted from the culture medium of USC by ultracentrifugation and identified. Cavernous sinus endothelial cells (CCEC) were collected from SD male rats and cultured in endothelial cell growth medium-2 (EGM-2) (the normal control group), EGM-2 + L-glucose at 25 mM (the high glucose group), EGM-2 + L-glucose at 25 mmol/L) + USC-Exo at 10 µg/ml (the Exo group), and EGM-2 + L-glucose at 25 mmol/L + USC-Exo at 10 µg/ml) + 3-methyladenine at 2 mmol/L (the 3-MA group), respectively. Changes of the autophagic flux in the CCECs transfected with mRFP-GFP-LC3 adenovirus were detected under the fluorescence microscope. The proliferation and tube-forming ability of the cells were assessed by CCK8 and Matrigel assays, respectively. DED was induced by intraperitoneal injection of streptozotocin in 10 of the rats, which were equally and randomly divided into a DED and an Exo group, and another 5 normal male rats were taken as controls. The rats in the normal and DED groups were injected intracavernously with 100 µl of PBS, and those in the Exo group with 100 µl of USC-Exo at the concentration of 1 µg/µl. Four weeks after treatment, the maximum intracavernous pressure (ICPmax) and mean arterial pressure (MAP) were measured, the endothelial marker CD31 detected by immunofluorescence assay, the expressions of the CD31, Beclin1 and LC3 I/II proteins examined by Western blot, and the number of autophagosomes in the cavernous endothelial cells determined under the transmission electron microscope. RESULTS: USC-Exo significantly increased the number of autophagosomes in the CCEC in the high glucose group compared with that in the normal controls (39.5 ± 6.2 vs 12.5 ± 5.4, P < 0.05). The expression of Beclin1 and proliferation of the CCEC were significantly higher in the Exo than in the high glucose group (P < 0.05). The autophagy inhibitor 3-MA evidently reversed the increasing effect of USC-Exo on the proliferation of the CCEC. The tube-forming ability of the CCEC was significantly increased in the Exo group compared with that in the high glucose group (15.3 ± 3.2 vs 6.3 ± 2.1, P < 0.05), which was also reversed in the 3-MA group. Both ICPmax and the ICPmax/MAP ratio were significantly higher in the Exo than in the DED group (ï¼»86.6 ± 12.6ï¼½ vs ï¼»37.9 ± 10.9ï¼½ mmHg, P < 0.05; 89.3 ± 14.1 vs 41.7 ± 11.5, P < 0.05), and so were the expressions of CD31, Beclin1 and LC3 I/II (P< 0.05) and the number of autophagosomes in the cavernosal endothelial cells (3.7 ± 0.6 vs 1.0 ± 1.0, P < 0.05). CONCLUSIONS: USC-Exo can significantly improve the endothelial and erectile functions of DED rats by increasing the autophagy of cavernosal endothelial cells.


Assuntos
Diabetes Mellitus , Disfunção Erétil , Exossomos , Humanos , Ratos , Masculino , Animais , Células Endoteliais/metabolismo , Proteína Beclina-1/metabolismo , Ratos Sprague-Dawley , Células-Tronco , Glucose/metabolismo
14.
Zhonghua Nan Ke Xue ; 28(2): 144-148, 2022 Feb.
Artigo em Zh | MEDLINE | ID: mdl-37462487

RESUMO

OBJECTIVE: To investigate the efficacy and safety of Compound Chamomile and Lidocaine Hydrochloride Gel (CCLH) (Kamistad) applied at different time-windows on premature ejaculation (PE). METHODS: This prospective study included 72 PE patients treated by application of CCLH to the glans and penile body in our hospital from February to October 2021. According to the time of drug administration before insertion into the vagina, we randomly divided the patients into a 5-minute group (n = 39) and a 15-minute group (n = 33). Before and after 1 and 2 weeks of treatment, we compared the intravaginal ejaculation latency time (IELT), PE diagnostic tool (PEDT) score, quality of life, and adverse reactions between the two groups of patients. RESULTS: Totally 62 of the patients completed the follow-up, 35 in the 5-minute group and 27 in the 15-minute group, and all showed significant improvement in IELT (P < 0.01) and PEDT score (P < 0.05) after treatment compared with the baseline. No allergic reactions, such as redness and swelling, developed at the application site in any of the patients, and no adverse significant effect was observed on the erectile hardness in 61 of the cases. Six cases showed increased erectile hardness instead. Fifty-seven of the patients experienced no obvious penile numbness or reduced sexual satisfaction, and all could complete their sexual activities. CONCLUSION: Compound Chamomile and Lidocaine Hydrochloride Gel applied at different time-windows is effective on PE, with a 5-minute rapid onset of action before intercourse, and no obvious adverse effects.


Assuntos
Ejaculação Precoce , Masculino , Humanos , Ejaculação Precoce/tratamento farmacológico , Ejaculação Precoce/induzido quimicamente , Lidocaína/uso terapêutico , Estudos Prospectivos , Camomila , Qualidade de Vida
15.
Org Biomol Chem ; 19(13): 2901-2906, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33725070

RESUMO

A copper-catalyzed direct C-H chalcogenation of N-aryl-azaindoles with disulfides is described. This transformation was performed using Earth abundant Cu(OAc)2 as a catalyst, benzoic acid as an additive, air as a terminal oxidant, and readily available diaryl and dialkyldisulfides (or diselenide) as chalcogenation reagents. High functional group tolerance and excellent regioselectivity are demonstrated by the efficient preparation of a wide range of ortho-sulfenylation-7-azaindoles.

16.
BMC Nephrol ; 22(1): 372, 2021 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-34753430

RESUMO

OBJECTIVE: To assess the clinical practicability of the ensemble learning model established by Liu et al. in estimating glomerular filtration rate (GFR) and validate whether it is a better model than the Asian modified Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation in a cohort of Chinese chronic kidney disease (CKD) patients in an external validation study. METHODS: According to the ensemble learning model and the Asian modified CKD-EPI equation, we calculated estimated GFRensemble and GFRCKD-EPI, separately. Diagnostic performance of the two models was assessed and compared by correlation coefficient, regression equation, Bland-Altman analysis, bias, precision and P30 under the premise of 99mTc-diethylenetriaminepentaacetic acid (99mTc-DTPA) dual plasma sample clearance method as reference method for GFR measurement (mGFR). RESULTS: A total of 158 Chinese CKD patients were included in our external validation study. The GFRensemble was highly related with mGFR, with the correlation coefficient of 0.94. However, regression equation of GFRensemble = 0.66*mGFR + 23.05, the regression coefficient was far away from one, and the intercept was wide. Compared with the Asian modified CKD-EPI equation, the diagnostic performance of the ensemble learning model also demonstrated a wider 95% limit of agreement in Bland-Altman analysis (52.6 vs 42.4 ml/min/1.73 m2), a poorer bias (8.0 vs 1.0 ml/min/1.73 m2, P = 0.02), an inferior precision (18.4 vs 12.7 ml/min/1.73 m2, P < 0.001) and a lower P30 (58.9% vs 74.1%, P < 0.001). CONCLUSIONS: Our study showed that the ensemble learning model cannot replace the Asian modified CKD-EPI equation for the first choice for GFR estimation in overall Chinese CKD patients.


Assuntos
Taxa de Filtração Glomerular , Aprendizado de Máquina , Insuficiência Renal Crônica/diagnóstico , Fatores Etários , Povo Asiático , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cintilografia , Compostos Radiofarmacêuticos , Análise de Regressão , Insuficiência Renal Crônica/diagnóstico por imagem , Fatores Sexuais , Pentetato de Tecnécio Tc 99m
17.
Eur Spine J ; 30(12): 3417-3427, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34476597

RESUMO

PURPOSE: To estimate the prevalence of depression in degenerative spine disease (DSD) patients. METHODS: The PubMed, EMBASE, and PsycINFO were systematically searched, the relevant studies that reported the depression prevalence of in DSD patients were identified. Data were extracted independently by 2 reviewers. Subgroup analysis and sensitivity analysis were also performed. RESULTS: 24 articles met the inclusion criteria and were selected for the current study. The pooled prevalence estimate of depression in DSD patients before operative treatment was 30.8% [95% CI 24.0-38.5%]. Nine articles reported the prevalence rate in DSD patients after operative treatment, and the pooled prevalence estimate was 27.0% [95% CI 19.9-35.4%]. There were significant differences for prevalence estimates before operative treatment in types of disorders (Q = 4.56, P = 0.10), spine surgery history (Q = 5.55, P = 0.02), representativeness of sample (Q = 11.00, P = 0.00), and validity of assessment method (Q = 3.32, P = 0.07). The prevalence estimates in patients with lumbar spine stenosis, lumbar disc herniation and cervical spondylotic myelopathy were 24.0%, 40.9% and 37.3%, respectively. Studies that included patients with a history of spine surgery yielded a more extreme prevalence estimate than studies excluding those (36.9% vs 24.3%). For results of patients after operative treatment, significant differences for prevalence estimates were showed in different degrees of pain (Q = 4.72, P = 0.03), screening instruments (Q = 4.83, P = 0.09), and representativeness of sample (Q = 15.70, P = 0.00). CONCLUSION: The systematic review indicated increased prevalence of depression in DSD patients. In consideration of the relationship between depression and poor surgical outcome, we should pay more attention to identifying strategies for preventing and treating depression in DSD patients.


Assuntos
Deslocamento do Disco Intervertebral , Doenças da Coluna Vertebral , Vértebras Cervicais , Depressão , Humanos , Prevalência , Doenças da Coluna Vertebral/epidemiologia , Doenças da Coluna Vertebral/cirurgia
18.
Int J Mol Sci ; 22(21)2021 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-34768789

RESUMO

Bone cancer including primary bone cancer and metastatic bone cancer, remains a challenge claiming millions of lives and affecting the life quality of survivors. Conventional treatments of bone cancer include wide surgical resection, radiotherapy, and chemotherapy. However, some bone cancer cells may remain or recur in the local area after resection, some are highly resistant to chemotherapy, and some are insensitive to radiotherapy. Phototherapy (PT) including photodynamic therapy (PDT) and photothermal therapy (PTT), is a clinically approved, minimally invasive, and highly selective treatment, and has been widely reported for cancer therapy. Under the irradiation of light of a specific wavelength, the photosensitizer (PS) in PDT can cause the increase of intracellular ROS and the photothermal agent (PTA) in PTT can induce photothermal conversion, leading to the tumoricidal effects. In this review, the progress of PT applications in the treatment of bone cancer has been outlined and summarized, and some envisioned challenges and future perspectives have been mentioned. This review provides the current state of the art regarding PDT and PTT in bone cancer and inspiration for future studies on PT.


Assuntos
Neoplasias Ósseas/tratamento farmacológico , Fototerapia/tendências , Ouro/farmacologia , Humanos , Nanopartículas/uso terapêutico , Recidiva Local de Neoplasia/tratamento farmacológico , Osteossarcoma/tratamento farmacológico , Fotoquimioterapia/métodos , Fotoquimioterapia/tendências , Fármacos Fotossensibilizantes/farmacologia , Fototerapia/métodos , Terapia Fototérmica/métodos , Terapia Fototérmica/tendências , Espécies Reativas de Oxigênio
19.
J Environ Manage ; 291: 112714, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-33940361

RESUMO

Organophosphate esters (OPEs) are typical industrial additives widely applied in various industrial and household products, but they may pollute drinking water. In the present study, the occurrence of OPEs in drinking water was analyzed. For this purpose, 25 drinking water samples were collected from households in 25 cities in China. The concentrations of OPEs were accurately determined according to a high-throughput organic analysis testing combined with high-volume solid phase extraction (Hi-throat/Hi-volume SPE) technique. Through this technique, nine OPEs were detected, with spiked recoveries from 77% to 101%, and their total concentrations (ΣOPEs) ranging from 46.8 to 251 (average 126) ng L-1. The detection frequencies of the selected OPEs determined via Hi-throat/Hi-volume SPE were 88.0%-100%, which are much higher than those previously reported. The limit of detections of the OPEs was low at < 0.01 ng L-1, which could be mainly attributed to the high volume of accumulated drinking water (>20 L). OPEs in drinking water presented a decreasing trend from cities in coastal provinces to inland cities of China. Redundancy analysis showed that the concentrations of OPEs in drinking water were significantly influenced by economic-demographic influencing factors. The non-carcinogenic and lifetime carcinogenic risks (non-CR and CR) of the analyzed OPEs in drinking water were negligible for urban residents in China.


Assuntos
Água Potável , Retardadores de Chama , China , Cidades , Monitoramento Ambiental , Ésteres/análise , Retardadores de Chama/análise , Humanos , Organofosfatos , Faringe/química
20.
Geriatr Nurs ; 42(4): 908-914, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34098444

RESUMO

OBJECTIVE: To investigate the feasible effect of a preoperative mobilization program for older hip fracture patients who waited more than two days before surgery. METHODS: A total of 38 patients with hip fracture were analyzed in this retrospective descriptive pilot study. The modified Barthel index (MBI) was used to measure functional outcome. Visual analogue scale (VAS) was used to evaluate the changes of pain degree during preoperative mobilization. The perioperative complications were recorded. RESULTS: After the preoperative mobilization program was implemented, the MBI score was improved immediately and further improved after surgery until 3 months after discharge. On the premise of analgesia, no patient experienced severe pain during preoperative mobilization. Perioperative complications occurred in 2 (5.3%) patients. CONCLUSION: For older patients with hip fracture, the preoperative mobilization program may be a feasible method, which may have a positive effect on promoting functional recovery and preventing perioperative complications.


Assuntos
Atividades Cotidianas , Fraturas do Quadril , Idoso , Fraturas do Quadril/cirurgia , Humanos , Projetos Piloto , Recuperação de Função Fisiológica , Estudos Retrospectivos , Resultado do Tratamento
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