Transcriptomic analysis of diapause-associated genes in Exorista civilis Rondani (Diptera:Tachinidae).

Exorista civilis Rondani (Diptera:Tachinidae) is an excellent dominant parasitic enemy all over the world. But there has been a lack of research on the molecular regulation of diapause in E. civilis. To investigate the important diapause-associated genes and metabolic pathways in E. civilis, we can provide a theoretical basis for clarifying the molecular mechanism of diapause at the transcriptome level. The Illumina HiSeq. 2000 platform was used to perform transcriptome sequencing and bioinformatics analysis of the non-diapause and diapause pupae of E. civilis. 58,050 unigenes were successfully assembled, in which 4355 upregulated and 3158 downregulated unigenes were differentially expressed. Moreover, by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichments, 896 kinds of the differentially expressed genes were specifically analyzed and showed that diapause-associated genes were related to be involved in the pathways of cold resistance, amino acid metabolism, and energy metabolism. Furthermore, these upregulated five genes showed the same trends of expression patterns between quantitative real-time polymerase chain reaction and RNA-Seq. This study provides a theoretical basis for the further study of the diapausing molecular mechanisms of E. civilis.

Functional Characterization of Pheromone Receptors in the Beet Webworm, Loxostege sticticalis (Lepidoptera: Pyralidae).

Lepidopteran insects mainly rely on sex pheromones to complete sexual communications. Pheromone receptors (PRs) are expressed on the olfactory receptor neurons (ORNs) of the sensilla trichodea and play an essential role in sexual communication. Despite extensive investigations into the mechanisms of peripheral recognition of sex pheromones in Lepidoptera, knowledge about these mechanisms in L. sticticalis remains limited. In this study, five candidate LstiPRs were analyzed in a phylogenetic tree with those of other Lepidopteran insects. Electroantennography (EAG) assays showed that the major sex pheromone component E11-14:OAc elicited a stronger antennal response than other compounds in male moths. Moreover, two types of neurons in sensilla trichodea were classified by single sensillum recordings, of which the "a" neuron specifically responded to E11-14:OAc. Five candidate PRs were functionally assayed by the heterologous expression system of Xenopus oocytes, and LstiPR2 responded to the major sex pheromone E11-14:OAc. Our findings suggest that LstiPR2 is a PR sensitive to L. sticticalis's major sex pheromone compound, E11-14:OAc. Furthermore, this study offers valuable insights into the sexual communication behavior of L. sticticalis, forming a foundation for further analysis of the species' central nervous system.

[Study on heavy metal contaminations and the sources of Pb pollution in Jinghai Bay using the stable isotope technique].

The concentrations of typical heavy metals including Cu, Hg, Pb, Zn, Mn, Cd, Cr and As in the surface sediment and suspended particulate matter (SPM) in Jinghai Bay, Rongcheng city were tested. The degree of heavy metal pollution in the surface sediment was assessed according to the national standard of marine sediment quality. The potential ecological risk (PER) of heavy metals to marine ecosystem in Jinghai Bay was assessed using PER coefficient and risk index. The results showed that the levels of heavy metals and PER in the surface sediments were low enough to meet the requirement of the first class of the sediment quality standards except the levels of Cu and Zn in the sampling site S2 and Cd in S3, S4, S5 and S6 which met the requirements of the second class of the sediment quality standards. The PER coefficients of heavy metals increased following the sequence of Zn < Cr < Pb < Cu < As

[Mistakes and countermeasures in diagnosis and treatment of prolapse of lumbar intervertebral disc].

The mistakes in clinical diagnosis and treatment due to dealing with symptoms, signs and imaging data in isolation, and unclear differentiation of relative diseases are found by reviewing definition and cause of disease and pathogenesis of prolapse of lumbar intervertebral disc, so as to probe into mistakes and countermeasures in diagnosis and treatment of prolapse of lumbar intervertebral disc. Only combined analysis of clinical symptoms, signs and imaging data, can correct diagnosis and treatment be made.

[Multicentral randomized controlled study on acupuncture at Sifeng (EX-UE 10) for treatment of infantile malnutrition].

OBJECTIVE: To evaluate therapeutic effect of acupuncture at Sifeng (EX-UE 10) on infantile malnutrition. METHODS: Multicentral, randomized, controlled and single blind test was adopted. 222 infants of malnutrition were divided into an acupuncture group (n=110) and a medicine group (n=112). The acupuncture group were treated with acupuncture at bilateral Sifeng (EX-UE 10), once each week, for 4 times; and the medicine group were treated with oral administration of Yiqi Jianpi Oral Liquid, twice each day, one ample each time, for 4 weeks. The therapeutic effect was evaluated by improvement of symptoms and signs in the syndrome cumulative score scale, and changes of serum insulin-like growth factor-I (IGF-I), pre-albumin (PA), hemoglobin and red-cell count. RESULTS: Two hundred and twenty-two cases were enrolled in the 4 centers and 212 cases completed the test. The acupuncture group in improvement of appetite, body weight, subcutaneous fat thickness of the abdomen, etc. were superior to the medicine group (P < 0.01), but there was no significant difference between the two groups in improvement of the body height. There was no significant increase of serum IGF-I level in the two groups, and the acupuncture group in increase of PA was superior to the medicine group (P < 0.05). After treatment, hemoglobin and red-cell count increased significantly in the treatment group (P < 0.01), and hemoglobin increased significantly in the medicine group. CONCLUSION: Acupuncture at Sifeng (EX-UE 10) has obvious therapeutic effect on infantile malnutrition.