Single hormone or synthetic agonist induces Gs/Gi coupling selectivity of EP receptors via distinct binding modes and propagating paths.

To accomplish concerted physiological reactions, nature has diversified functions of a single hormone at at least two primary levels: 1) Different receptors recognize the same hormone, and 2) different cellular effectors couple to the same hormone-receptor pair [R.P. Xiao, Sci STKE 2001, re15 (2001); L. Hein, J. D. Altman, B.K. Kobilka, Nature 402, 181-184 (1999); Y. Daaka, L. M. Luttrell, R. J. Lefkowitz, Nature 390, 88-91 (1997)]. Not only these questions lie in the heart of hormone actions and receptor signaling but also dissecting mechanisms underlying these questions could offer therapeutic routes for refractory diseases, such as kidney injury (KI) or X-linked nephrogenic diabetes insipidus (NDI). Here, we identified that Gs-biased signaling, but not Gi activation downstream of EP4, showed beneficial effects for both KI and NDI treatments. Notably, by solving Cryo-electron microscope (cryo-EM) structures of EP3-Gi, EP4-Gs, and EP4-Gi in complex with endogenous prostaglandin E2 (PGE2)or two synthetic agonists and comparing with PGE2-EP2-Gs structures, we found that unique primary sequences of prostaglandin E2 receptor (EP) receptors and distinct conformational states of the EP4 ligand pocket govern the Gs/Gi transducer coupling selectivity through different structural propagation paths, especially via TM6 and TM7, to generate selective cytoplasmic structural features. In particular, the orientation of the PGE2 ω-chain and two distinct pockets encompassing agonist L902688 of EP4 were differentiated by their Gs/Gi coupling ability. Further, we identified common and distinct features of cytoplasmic side of EP receptors for Gs/Gi coupling and provide a structural basis for selective and biased agonist design of EP4 with therapeutic potential.

Structures of the ADGRG2-Gs complex in apo and ligand-bound forms.

Adhesion G protein-coupled receptors are elusive in terms of their structural information and ligands. Here, we solved the cryogenic-electron microscopy (cryo-EM) structure of apo-ADGRG2, an essential membrane receptor for maintaining male fertility, in complex with a Gs trimer. Whereas the formations of two kinks were determinants of the active state, identification of a potential ligand-binding pocket in ADGRG2 facilitated the screening and identification of dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate and deoxycorticosterone as potential ligands of ADGRG2. The cryo-EM structures of DHEA-ADGRG2-Gs provided interaction details for DHEA within the seven transmembrane domains of ADGRG2. Collectively, our data provide a structural basis for the activation and signaling of ADGRG2, as well as characterization of steroid hormones as ADGRG2 ligands, which might be used as useful tools for further functional studies of the orphan ADGRG2.

Performance improvement of underwater LIBS qualitative and quantitative analysis by irradiating with long nanosecond pulses.

Long nanosecond pulses have been proven to be efficient at enhancing underwater LIBS emission. However, the quantitative analytical capability of underwater long-pulse LIBS has yet to be further revealed. In this work, we investigated the spectral characteristics by irradiating with a laser pulse of 120 ns duration. The alkali and alkaline earth metals Li, K and Ca and the transition element Mn were selected for analysis. It is shown that obvious self-reversal structures were observed in the spectra at high concentrations, making the calibration curves saturated. Correction was performed using the approximate Voigt function fitting method, which significantly improves the linearity of the calibration curves. In addition to the target metal elements, atomic lines of the matrix elements H and O in water were also observed, which can serve as promising internal standards for quantitative analysis. A comparison of the quantification performance with and without the internal standards demonstrates that the use of the internal standards is conducive to improving the robustness of the calibration approaches with higher determination coefficients. More importantly, the underwater LIBS signal stability is improved by more than 3 times, and the prediction error for validation samples is reduced by 2-4 times. The present results suggest that long ns pulses are favorable to significantly improving the qualitative and quantitative performance of underwater single-pulse LIBS, enabling long-pulse LIBS to have great potential to be applied to underwater in situ chemical analysis.

Performance Study of F-P Pressure Sensor Based on Three-Wavelength Demodulation: High-Temperature, High-Pressure, and High-Dynamic Measurements.

F-P (Fabry-Perot) pressure sensors have a wide range of potential applications in high-temperature, high-pressure, and high-dynamic environments. However, existing demodulation methods commonly rely on spectrometers, which limits their application to high-frequency pressure signal acquisition. To solve this problem, this study developed a self-compensated, three-wavelength demodulation system composite with an F-P pressure sensor and a thermocouple to construct a comprehensive sensing system. The system produces accurate pressure measurements in high-temperature, high-pressure, and high-dynamic environments. In static testing at room temperature, the sensing system shows excellent linearity, and the pressure sensitivity is 158.48 nm/MPa. In high-temperature testing, the sensing system maintains high linearity in the range of 100 °C to 700 °C, with a maximum pressure-indication error of about 0.13 MPa (0~5 MPa). In dynamic testing, the sensor exhibits good response characteristics at 1000 Hz and 5000 Hz sinusoidal pressure frequencies, with a signal-to-noise ratio (SNR) greater than 37 dB and 45 dB, respectively. These results indicate that the sensing system proposed in this study has significant competitive advantages in the field of high-temperature, high-speed, and high-precision pressure measurements and provides an important experimental basis and theoretical support for technological progress in related fields.

High-efficiency FBG array sensor interrogation system via a neural network working with sparse data.

FBG array sensors have been widely used in the multi-point monitoring of large structures due to their excellent optical multiplexing capability. This paper proposes a cost-effective demodulation system for FBG array sensors based on a Neural Network (NN). The stress variations applied to the FBG array sensor are encoded by the array waveguide grating (AWG) as transmitted intensities under different channels and fed to an end-to-end NN model, which receives them and simultaneously establishes a complex nonlinear relationship between the transmitted intensity and the actual wavelength to achieve absolute interrogation of the peak wavelength. In addition, a low-cost data augmentation strategy is introduced to break the data size bottleneck common in data-driven methods so that the NN can still achieve superior performance with small-scale data. In summary, the demodulation system provides an efficient and reliable solution for multi-point monitoring of large structures based on FBG array sensors.

Enhanced wide-range gas pressure sensing with an all-solid open Fabry-Pérot interferometer.

The sensors with a wide gas pressure detection range are urgently demanded in many industrial applications. Here, we propose a gas pressure sensor based on an all-solid open Fabry-Pérot interferometer, which is prepared by using optical contact bonding to ensure high structural strength and high-quality factor of 8.8 × 105. The applied pressure induces a change in the refractive index of the air, leading to the shift of the resonant spectrum. The pressure is detected by calibrating this shift. The sensor exhibits a pressure sensitivity of 4.20 ± 0.01 nm/MPa in a pressure range of 0 to 10 MPa and has a minimum pressure resolution of 0.005 MPa. Additionally, it shows a lower temperature cross-sensitivity of -0.25 kPa/°C. These findings affirm that the sensor achieves high-sensitivity pressure sensing across a wide detection range. Moreover, owing to its exceptional mechanical strength, it holds great promise for applications in harsh environments, such as high temperature and high pressure.

Fabrication and Performance of a Ta2O5 Thin Film pH Sensor Manufactured Using MEMS Processes.

In this work, a capacitive pH sensor consisting of Ta2O5 functional film is designed and fabricated by employing MEMS-based procedures. The Ta2O5 thin film has an amorphous microstructure, and its surface roughness is less than 3.17 nm. A signal processing circuit and a software filtering algorithm are also designed to measure the pH value, thus improving the detection accuracy and anti-interference ability. Good linearity (R2 = 0.99904) and sensitivity (63.12 mV/pH) are recorded for the proposed sensing element in the range of pH 2~12. In addition, the sensor's drift and hysteresis are equal to 5.1 mV and 5.8 mV, respectively. The enhanced sensing performance in combination with the facile miniaturization process, low fabrication cost, and suitability for mass production render the fabricated sensor attractive for applications where pH change measurements in a water environment are required.

Understanding work experience in epidemic-induced telecommuting: The roles of misfit, reactance, and collaborative technologies.

In the management of the global COVID-19 pandemic, the mandated closure of workplaces and stay-at-home orders have forced workers to adapt to a prolonged period of unplanned telecommuting, which we term epidemic-induced telecommuting. Although epidemic-induced telecommuting has drastically altered how work is conducted, scant attention is being paid to this emerging work arrangement. To this end, we combine psychological reactance theory and person-environment fit theory to advance the concept of misfit between worker and environment as a core determinant of employees' work experience in the epidemic-induced telecommuting. Particularly, we distinguish between supply-value and demand-ability misfits as constraints on workers' freedom at work. Having analyzed data collected through a survey administered on remote workers, we discovered that both misfits positively influenced workers' perceived psychological reactance, which led to work exhaustion and counter-productive behaviors. We also found that the utilization of collaborative technologies moderated the effects of misfit on workers' psychological reactance.

Research on high-temperature characteristics of a miniature Fabry-Pérot cavity acoustic sensor.

The applications of fiber-optic acoustic sensors are expanded to the high-temperature field, but it still faces challenges to realize the wide-band and high-sensitivity acoustic signal detection in high-temperature environments. Here, we propose a miniature membrane-free fiber-optic acoustic sensor based on a rigid Fabry-Pérot (F-P) cavity and construct an acoustic signal detection system. The system can achieve high-sensitivity acoustic detection while maintaining a wide frequency band in temperatures ranging from 20 °C to 200 °C. The prepared F-P cavity based on optical contact technology is the sensitive unit of the sensor, and has a high-quality factor of 8.8×105. Specifically, with the increasing of temperature, the sensitivity gradually increases, and the frequency response range does not change. A maximum sensitivity of 491.2 mV/Pa and a high signal-to-noise ratio of 60.9 dB are achieved at 200 °C. The sensor has an excellent acoustic signal response in the frequency range of 10 Hz-50 kHz with a flatness of ±2 dB. This study is important for the application of the fiber-optic acoustic sensor in high-temperature environments.

Compact fiber-optic Fabry-Perot cavity based on sandwich structure adopting direct bonding of quartz glass.

A compact fiber-optic Fabry-Perot (F-P) cavity for a sensor is designed based on a sandwich structure, adopting direct bonding of quartz glass. The reflective F-P cavity is manufactured by a fiber optic with a quartz glass ferrule and the sandwich structure with an air cavity, which is achieved by direct bonding of quartz glass. This fabrication process includes plasma surface activation, hydrophilic pre-bonding, high-temperature annealing, and dicing. The cross section of the bonding interface tested by a scanning electron microscope indicates that the sandwich structure is well bonded, and the air cavity is not deformed. Experiments show that the quality factor of the F-P cavity is 2711. Tensile strength testing shows that the bond strength exceeds 35 MPa. The advantage of direct bonding of quartz glass is that high consistency and mass production of the cavity can be realized. Moreover, the cavity is free of problems caused by the mismatch of thermal expansion coefficients between different materials. Therefore, the F-P cavity can be made into a sensor, which is promising in detecting air pressure, acoustic and high temperature.

Phytoremediation potential of hybrids of the exotic plant Xanthium strumarium and its native congener Xanthium sibiricum for cadmium-contaminated soils.

Exotic plants could play an essential role in the restoration of heavy metal-contaminated soil. This study evaluated the tolerance of and extraction of cadmium (Cd) by ZCR (CR♀ × LT♂), hybrids of Xanthium strumarium (LT, exotic species) and X. sibiricum (CR, indigenous congener), and their parental species under different Cd treatments (0, 10, 40, and 80 mg·kg-1). The results showed that the hybrids had significantly improved tolerance to Cd. Under Cd stress, the biomass of ZCR increased by more than 50% on average compared with that of CR. Moreover, the hybrids showed a more remarkable ability to transport Cd from the root to the shoot. The Cd content of the shoots of ZCR increased by 128.33, 147.22, and 252.63% when treated with 10, 40, and 80 mg·kg-1 Cd, respectively. ZCR stored more than 70% of Cd in litter leaves, thereby reducing the toxic effects of Cd on photosynthesis and growth. The results showed that ZCR showed excellent Cd tolerance and enrichment in the presence of Cd. The hybrids of Xanthium strumarium and its native congener X. sibiricum may remediate soil Cd pollution.Novelty statementWith the changing world economy and increasing human activities, exotic plants have become a global issue of common concern to the international community. This study describes new findings on using hybrids of the exotic plant of Xanthium strumarium and its native congener Xanthium sibiricum for the restoration of cadmium-contaminated soils. Under Cd stress, the hybrids' biomass, tolerance, and ability to accumulate Cd were significantly higher than that of X. sibiricum, indicating that hybrids gained useful heavy metal extraction traits from X. strumarium.

An Optimal Design Method for Improving the Efficiency of Ultrasonic Wireless Power Transmission during Communication.

Due to the excellent directivity, strong penetrability, and no electromagnetic shielding effect, ultrasonic waves have good potential for wireless energy transmission and information transfer inside and outside of sealed metal devices. However, traditional ultrasonic based energy transmission methods usually result in considerable energy consumption because of the impedance mismatch during the impedance modulation of the communication. This paper presents an optimal design method for efficient energy transfer during ultrasonic communication. The channel equivalent circuit model is established by only using the acoustic-electric channel scattering parameters. According to the equivalent circuit model, the channel impedance matches with a weak mismatch state is performed during the communication. In this way, the impedance modulation effect is ensured with a lower decrease in the energy transmission efficiency. Finally, the simultaneous energy transmission and impedance modulation are carried out through the 11 mm thick 304 stainless steel plate. The transmission power is 37.86 W with a transmission efficiency of 45.75%, and the modulation rate is 10 Kbps. Compared with the traditional methods, our proposed energy transmission efficiency is increased by 17.62%. The results verify the proposed method's effectiveness and the high accuracy of the model. The proposed method has great engineering applications and broad prospects in condition monitoring of metallic environments.

A Long-Circulating Vector for Aptamers Based upon Polyphosphodiester-Backboned Molecular Brushes.

Aptamers face challenges for use outside the ideal conditions in which they are developed. These difficulties are most palpable in vivo due to nuclease activities, rapid clearance, and off-target binding. Herein, we demonstrate that a polyphosphodiester-backboned molecular brush can suppress enzymatic digestion, reduce non-specific cell uptake, enable long blood circulation, and rescue the bioactivity of a conjugated aptamer in vivo. The backbone along with the aptamer is assembled via solid-phase synthesis, followed by installation of poly(ethylene glycol) (PEG) side chains using a two-step process with near-quantitative efficiency. The synthesis allows for precise control over polymer size and architecture. Consisting entirely of building blocks that are generally recognized as safe for therapeutics, this novel molecular brush is expected to provide a highly translatable route for aptamer-based therapeutics.

Micro-fiber-optic acoustic sensor based on high-Q resonance effect using Fabry-Pérot etalon.

A micro-fiber-optic acoustic sensor based on the high-quality-factor (high-Q) resonance effect that uses a Fabry-Pérot etalon (FPE) is presented in this study. The device has been demonstrated experimentally to be a high-sensitivity acoustic sensor with a large dynamic range over a wide frequency band. Optical contact technology was used to improve the robustness of the FPE, which consists of two parallel lenses with high reflectivity exceeding 99%. An acoustic signal detection system based on phase modulation spectrum technology was also constructed. A stable and high-Q value of 106 was measured for the FPE. As a result, high sensitivity of 177.6 mV/Pa was achieved. Because of the change in the refractive index of the air when it is modulated by the acoustic waves, a frequency response of 20 Hz-70 kHz with flatness of ±2 dB was obtained and a large dynamic range of 115.3 dB was measured simultaneously. The excellent performance of the device will be beneficial for optical acoustic sensing.

Socioeconomic factors and regional differences of PM2.5 health risks in China.

China is a country with one of the highest concentrations of airborne particulate matter smaller than 2.5 µm (PM2.5) in the world, and it has obvious spatial-distribution characteristics. Areas of concentrated population tend to be regions with higher PM2.5 concentrations, which further aggravate the impact of PM2.5 pollution on population health. Using PM2.5-concentration and socioeconomic data for 225 cities in China in 2015, we adopted a PM2.5-health-risk-assessment method (with simplified calculation) and applied the Stochastic Impacts by Regression on Population, Affluence, and Technology (STIRPAT) model to analyze the effects of socioeconomic factors on PM2.5 health risks. The results showed that: (1) At the national level, the order of contribution degree of each socioeconomic factor in the PM2.5-health-risk and PM2.5-concentration model is consistent. (2) From a regional perspective, in all three regions, the industrial structure is the decisive factor affecting PM2.5 health risks, and reduction of energy intensity increases PM2.5 health risks, but the impact of the total amount of urban central heating on PM2.5 health risks is very low. In the eastern region, the increased urbanization rate and length of highways significantly increase PM2.5 health risks, but the increasing effect of the extent of built-up area is the lowest. In the central region, the increasing effects of the extent of built-up area on PM2.5 health risks are significantly greater than the decreasing effects of the urbanization rate. In the western region, economic development has the least effect on reducing PM2.5 health risks. Our research enriches PM2.5-health-risk theory and provides some theoretical support for PM2.5-health-risk diversity management in China.

Filtering effect of SiO2 optical waveguide ring resonator applied to optoelectronic oscillator.

Single-mode oscillation is crucial to the practicality of optoelectronic oscillator (OEO). Due to the limited by bandwidth and precision of radio frequency (RF) filters, it is difficult to be achieved for the OEO based on the long fiber-optic delay line. So instead of the long fiber-optic delay line, SiO2 optical waveguide ring resonator (OWRR) with high-Q and mode selection is first presented to be applied to OEO. The OEOs based on the minimum loop and SiO2 OWRR are constructed. The oscillation characteristics of the minimum loop OEO and the transmission characteristics of the SiO2 OWRR are simulated by MATLAB, respectively. The filtering effect of the SiO2 OWRR applied to the OEO is verified theoretically by comparing these simulation results. Subsequently, the contrastive experiments of the above two OEOs on oscillation modes are carried out. The oscillation mode spacing of 40.32 MHz and 2.137 GHz are obtained. These results show that the SiO2 OWRR can function as an excellent 'filter' in the minimum loop of the OEO. Moreover, the side mode suppression ratio and the phase noise of the OEO have been improved. Our experimental results demonstrate that the OEO adopting SiO2 OWRR is feasible to achieve the single-mode oscillation and obtain better performance microwave signals.

A Mathematical Model of a Piezo-Resistive Eight-Beam Three-Axis Accelerometer with Simulation and Experimental Validation.

A mathematical model of a sensor is vital to deeply comprehend its working principle and implement its optimal design. However, mathematical models of piezo-resistive eight-beam three-axis accelerometers have rarely been reported. Furthermore, those works are largely focused on the analysis of sensing acceleration in the normal direction, rather than in three directions. Therefore, a complete mathematical model of a piezo-resistive eight-beam three-axis accelerometer is developed in this paper. The validity of the mathematical model is proved by a Finite Element Method (FEM) simulation. Furthermore, the accelerometer is fabricated and tested. The prime sensitivities of X, Y and Z axes are 0.209 mV/g, 0.212 mV/g and 1.247 mV/g at 160 Hz, respectively, which is in accord with the values obtained by the model. The reason why the prime sensitivity SZZ is bigger than SXX and SYY is explained. Besides, it is also demonstrated why the cross-sensitivities SXZ and SYZ exceed SZX and SZY. Compared with the FEM model, the developed model could be helpful in evaluating the performance of three-axis accelerometers in an accurate and rapid way.

The Application of an Ultrasound Tomography Algorithm in a Novel Ring 3D Ultrasound Imaging System.

Currently, breast cancer is one of the most common cancers in women all over the world. A novel 3D breast ultrasound imaging ring system using the linear array transducer is proposed to decrease costs, reduce processing difficulties, and improve patient comfort as compared to modern day breast screening systems. The 1 × 128 Piezoelectric Micromachined Ultrasonic Transducer (PMUT) linear array is placed 90 degrees cross-vertically. The transducer surrounds the mammary gland, which allows for non-contact detection. Once the experimental platform is built, the breast model is placed through the electric rotary table opening and into a water tank that is at a constant temperature of 32 °C. The electric rotary table performs a 360° scan either automatically or mechanically. Pulse echo signals are captured through a circular scanning method at discrete angles. Subsequently, an ultrasonic tomography algorithm is designed, and a horizontal slice imaging is realized. The experimental results indicate that the preliminary detection of mass is realized by using this ring system. Circular scanning imaging is obtained by using a rotatable linear array instead of a cylindrical array, which allows the size and location of the mass to be recognized. The resolution of breast imaging is improved through the adjustment of the angle interval (>0.05°) and multiple slices are gained through different transducer array elements (1 × 128). These results validate the feasibility of the system design as well as the algorithm, and encourage us to implement our concept with a clinical study in the future.

Plasmon-enhanced sensitivity of spin-based sensors based on a diamond ensemble of nitrogen vacancy color centers.

A method for enhancement of the sensitivity of a spin sensor based on an ensemble of nitrogen vacancy (NV) color centers was demonstrated. Gold nanoparticles (NPs) were deposited on the bulk diamond, which had NV centers distributed on its surface. The experimental results demonstrate that, when using this simple method, plasmon enhancement of the deposited gold NPs produces an improvement of ∼10 times in the quantum efficiency and has also improved the signal-to-noise ratio by approximately ∼2.5 times. It was also shown that more electrons participated in the spin sensing process, leading to an improvement in the sensitivity of approximately seven times; this has been proved by Rabi oscillation and optical detection of magnetic resonance (ODMR) measurements. The proposed method has proved to be a more efficient way to design an ensemble of NV centers-based sensors; because the result increases in the number of NV centers, the quantum efficiency and the contrast ratio could greatly increase the device's sensitivity.

Refractive Index Sensor Based on Fano Resonances in Metal-Insulator-Metal Waveguides Coupled with Resonators.

A surface plasmon polariton refractive index sensor based on Fano resonances in metal-insulator-metal (MIM) waveguides coupled with rectangular and ring resonators is proposed and numerically investigated using a finite element method. Fano resonances are observed in the transmission spectra, which result from the coupling between the narrow-band spectral response in the ring resonator and the broadband spectral response in the rectangular resonator. Results are analyzed using coupled-mode theory based on transmission line theory. The coupled mode theory is employed to explain the Fano resonance effect, and the analytical result is in good agreement with the simulation result. The results show that with an increase in the refractive index of the fill dielectric material in the slot of the system, the Fano resonance peak exhibits a remarkable red shift, and the highest value of sensitivity (S) is 1125 nm/RIU, RIU means refractive index unit. Furthermore, the coupled MIM waveguide structure can be integrated with other photonic devices at the chip scale. The results can provide a guide for future applications of this structure.