RESUMO
This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal).. This article has been retracted at the request of < the Editor in Chief. The Editor in Chief has been made aware of numerous problems with this paper regarding authorship, poor or insufficient supervision of researchers and the unauthorized use of data acquired from a lab visit by one of the authors.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Proteína com Dedos de Zinco da Leucemia Promielocítica/genética , Linfócitos T/citologia , Animais , Contagem de Células , Autorrenovação Celular , Deleção de Genes , Camundongos , Camundongos Endogâmicos C57BL , Linfócitos T/metabolismo , Timócitos/citologia , Timócitos/metabolismo , Timo/citologia , Timo/crescimento & desenvolvimento , Timo/metabolismoRESUMO
Kabuki syndrome (KS) is a multiple congenital anomaly/mental retardation syndrome with characteristic facial features. Despite more than 350 documented cases and recent correlation of MLL2 mutations as a genetic cause, its full clinical spectrum is still being defined. This report describes two patients who were initially diagnosed with Van der Woude syndrome (VWS) based on the presence of lower lip pits. However, this finding can occur with KS, albeit infrequently. For patients with lower lip pits, a thorough evaluation should be made to distinguish between VWS and KS, as there are differences in long-term prognosis.
Assuntos
Anormalidades Múltiplas/diagnóstico , Anormalidades Múltiplas/cirurgia , Fenda Labial/diagnóstico , Fissura Palatina/diagnóstico , Cistos/diagnóstico , Face/anormalidades , Doenças Hematológicas/diagnóstico , Doenças Hematológicas/cirurgia , Lábio/anormalidades , Doenças Vestibulares/diagnóstico , Doenças Vestibulares/cirurgia , Diagnóstico Diferencial , Face/cirurgia , Feminino , Humanos , Lactente , MasculinoRESUMO
Keratoacanthoma (KA) is a self-regressing cutaneous tumor sharing clinical and histopathological similarities with squamous cell carcinoma. It is relatively uncommon and presents diagnostic challenges, particularly in pediatric patients. This paper presents a case study of KA affecting the upper lip keratosis in a 6-year-old girl. Through a comprehensive analysis of the pathogenesis, differential diagnosis, and treatment approaches of KA in children based on existing literature, we aim to offer clinical insights and guidance for practice.
RESUMO
Hamartoma is a congenital benign lesion commonly found in the lungs, kidneys, colon, and other regions, but it is seldom seen in the oral cavity. Multiple hamartoma occurrences in the tongue are particularly rare. This article describes a 7-day-old female infant with multiple tongue tumors and a cleft palate, who had difficulty feeding and subsequently underwent tumor removal under general anesthesia. Nine months later, a cleft palate repair was performed. No genetic abnormalities were detected in the genetic testing. After the tumor removal, follow-ups were conducted every year to observe any recurrence of the tumors, the morphology and function of the tongue, and any systemic abnormalities. After 7 years of follow-up, there was no recurrence of the tumors, and the morphology and function of the tongue were normal, with no systemic diseases found. It is crucial to conduct multidisciplinary consultations for children diagnosed with multiple tongue hamartomas and to monitor their overall development while addressing oral lesions.
RESUMO
A bronchogenic cyst (BC) is a rare congenital cystic lesion, especially when located in the suprahyoid region of the anterior cervical front. We report a case of a male child who was misdiagnosed with a thyroglossal duct cyst due to the clinical presentation of a mass located near the anterior midline of the neck, in the region above the hyoid bone, which moved up and down with swallowing, characteristics consistent with a thyroglossal duct cyst. We excised the lesion under general anesthesia. Histopathological analysis revealed cartilage tissue, pseudostratified columnar epithelium of the cyst wall with cilia and mucous glands, finally diagnosing it as BC. BC should be considered in the differential diagnosis of neck masses.
RESUMO
Congenital granular cell epulis (CGCE) is a rare disease, particularly when it affects multiple oral and maxillofacial regions. We present the case of a female neonate with 4 asymptomatic masses attached to the maxillary and mandibular gingiva as well as the tongue. Due to the size of the masses causing feeding and respiratory difficulties, lesion removal was performed under general anesthesia. Histopathological analysis revealed tightly arranged polygonal cells with abundant granular eosinophilic cytoplasm. Immunohistochemically, the cells were negative for Smur100 (S-100), neuron-specific enolase (NSE), cluster of differentiation (CD) 68, and Sry-related HMG box (SOX)-10, and positive for alpha-1-antitrypsin (AAT) and vimentin. The diagnosis of multiple CGCEs was established through clinical examinations, histomorphometrical, and immunohistochemical analyses. In conclusion, CGCE should be considered in the differential diagnosis of intraoral lesions in neonates.
RESUMO
Dentigerous cyst belongs to one kind of odontogenic cysts, and is also known as follicular cyst. After the formation of the crown or root of the tooth, liquid exudates between the residual enamel epithelium and the crown surface to form odontogenic cysts. Multiple odontogenic cysts are rare in the oral and maxillofacial regions, especially in different areas of the jaw. In this paper, we reported case with multiple odontogenic cysts and discussed its etiology,pathological classification,differential diagnosis and treatment.
Assuntos
Cisto Dentígero , Cistos Odontogênicos , Cisto Dentígero/diagnóstico , Cisto Dentígero/cirurgia , Epitélio , Humanos , Mandíbula , Cistos Odontogênicos/diagnóstico , Cistos Odontogênicos/cirurgia , Coroa do DenteRESUMO
Hepatitis A virus (HAV) belongs to the family Picornaviridae. It is the pathogen of acute viral hepatitis caused by fecal-oral transmission. RNA viruses are sensed by pathogen-associated pattern recognition receptors (PRRs) such as Toll-like receptor 3 (TLR3), retinoic acid-inducible gene I (RIG-I), and melanoma differentiation-associated gene 5 (MDA5). PRR activation leads to production of type 1 interferon (IFN-α/ß), serving as the first line of defense against viruses. However, HAV has developed various strategies to compromise the innate immune system and promote viral propagation within the host cells. The long coevolution of HAV in hosts has prompted the development of effective immune antagonism strategies that actively fight against host antiviral responses. Proteases encoded by HAV can cleave the mitochondrial antiviral signaling protein (MAVS, also known as IPS-1, VISA, or Cardif), TIR domain- containing adaptor inducing IFN-ß (TRIF, also known as TICAM-1) and nuclear factor-κB (NF-κB) essential modulator (NEMO), which are key adaptor proteins in RIG-I-like receptor (RLR), TLR3 and NF-κB signaling, respectively. In this mini-review, we summarize all the recent progress on the interaction between HAV and the host, especially focusing on how HAV abrogates the antiviral effects of the innate immune system.
RESUMO
It is important to isolate potential candidates from the local isolates of nematophagous fungi and to investigate interaction between the fungal strains and gastrointestinal nematodes for the biological control of parasitic nematodes in livestock. In the present study, we assessed the in vitro predatory activity and the viability of isolates of Arthrobotrys thaumasia ( Monacrosporium thaumasium) after passage through the gastrointestinal tract of sheep. The predatory process of a representative isolate selected against the larvae of trichostrongylids was prepared with a scanning electron microscope (SEM). In vitro experiments tested the ability of 9 native isolates of A. thaumasia to prey on larvae of feces of sheep infected with natural mixed nematodes ( Haemonchus contortus, Trichostongylus colubriformis, Marshallagia mongolica). These isolates of A. thaumasia decreased infectivity of third stage infective larvae (L3) by 75.54-99.97%; 7 isolates decreased infectivity by more than 90%. In vivo experiments also demonstrated significant reductions of L3 numbers in the feces treated with the 9 isolates after passing through the gastrointestinal tract of sheep, and these decreases ranged from 51.68 to 88.16%. The isolates tested were re-isolated in 5-g sub-samples of feces from sheep in each treatment group, indicating that these isolates had the capacity to prey upon larvae of trichostrongylids after the passage through gastrointestinal tract. SEM shows that at 6 hr after the larvae were added, including the second stage larvae (L2) and L3 of trichostrongylids, the isolate NBS 005 caught them; at 8 hr after being caught L2 was penetrated by the fungus while penetration of L3 occurred at 12 hr; at 78 hr post-capture L2 was completely destroyed by the fungus while complete digestion of L3 occurred at 84 hr.
Assuntos
Ascomicetos/fisiologia , Trichostrongyloidea/microbiologia , Análise de Variância , Animais , Ascomicetos/ultraestrutura , Resistência a Múltiplos Medicamentos , Fezes/parasitologia , Trato Gastrointestinal/microbiologia , Trato Gastrointestinal/parasitologia , Larva/microbiologia , Gado , Masculino , Microscopia Eletrônica de Varredura , Controle Biológico de Vetores , Distribuição Aleatória , Ovinos , Trichostrongyloidea/efeitos dos fármacos , Trichostrongyloidea/ultraestruturaRESUMO
Multiple myeloma is the most common type of multifocal plasma cell proliferation in the bone marrow, whereas cases with multiple amyloidosis of oral cavity are rarely documented. Clinically, the disease is easily miss- diagnosed and mistreated due to lack of specificity. This article reported a rare case with multiple myeloma and amyloidosis of tongue, gingiva, buccal mucosa, followed by a review of relevant literature.
Assuntos
Amiloidose/diagnóstico , Mieloma Múltiplo/diagnóstico , Amiloidose/terapia , Medula Óssea , Proliferação de Células , Gengiva , Humanos , Mucosa Bucal , Mieloma Múltiplo/terapia , LínguaRESUMO
PURPUSE: To investigate the distribution of non-thyroid and non-salivary gland neck lesions, in order to improve the diagnosis of cervical masses. METHODS: Eight hundred and seventeen patients with neck mass treated in our department between 2000 and 2013 were collected for retrospective analysis. RESULTS: There were 236 inflammatory masses, 276 congenital masses, 99 benign tumors, 80 primary malignant tumors and 126 metastatic malignant tumors, which accounted for 28.89%, 33.78%, 12.11%, 9.79% and 15.42%, respectively. The clinical characteristics were different among different lesions. CONCLUSIONS: Neck masses are in various forms. To understand the characteristics and patterns of onset, appropriate preoperative examination and multi-parameter comprehensive diagnosis is the key to improve diagnostic rate.
Assuntos
Neoplasias de Cabeça e Pescoço/epidemiologia , China/epidemiologia , Neoplasias de Cabeça e Pescoço/diagnóstico , Humanos , Pescoço/anormalidades , Estudos RetrospectivosRESUMO
The aim of the present study was to investigate the generation of cell sheet-engineered bones used for the reconstruction of mandibular defects. Bone marrow stem cells (BMSCs) were cultured and induced to generate osteoblasts. Poly(lactic-co-glycolic acid) (PLGA) scaffolds were wrapped with or without cell sheets and then implanted into dogs with mandibular defects in the right side (experimental group) or the left side (control group), respectively. Subsequently, X-ray analyses, and hematoxylin and eosin staining were performed at various time points (at 4, 8, 12 or 16 weeks post-implantation; n=4 at each time point). The osteogenesis in the experimental group was significantly improved compared with that in the control group. At 16 weeks after implantation, numerous Haversian systems and a few lamellar bones were observed at the periphery. In the control group, the engineered bone (without BMSC sheets) presented fewer Haversian systems and no lamellar bones. The optical density of the fresh bone in the experimental group was significantly higher compared with that in the control group (P<0.05). In conclusion, tissue-engineered bone with the structure of lamellar bones can be generated using BMSC sheets and implantation of these bones had an improved effects compared with the control group. Cell sheet transplantation was found to enhance bone formation at the reconstruction site of the mandibular defects.
RESUMO
AIM: The purpose of this study was to determine whether susceptibility to oral tongue squamous cell carcinoma (OSCC) is related to polymorphisms in the u-PA gene. METHODS: We examined the rs2227564 C/T and rs2227562 G/A single nucleotide polymorphisms (SNPs) in 196 OSCC patients and 201 age- and gender- matched controls via direct sequencing and PCR-RFLP methods. RESULTS: Significant differences were found in allelic and genotypic distributions of the rs2227564 and rs2227562 loci when comparing cases and controls. In addition, logistic analyses indicated that the rs2227564 C/T genotype was related to a 1.52-fold increased risk of developing OSCC (adjusted OR=1.521, 95%CI: 1.144~2.022, P=0.004). Linkage disequilibrium analysis was conducted and no association between the two loci was found (D'=0.031, r2=0.000). CONCLUSIONS: Our findings provide evidence that the rs2227564 C/T SNP in the u-PA gene is associated with the development of OSCC.
Assuntos
Carcinoma de Células Escamosas/genética , Neoplasias da Língua/genética , Ativador de Plasminogênio Tipo Uroquinase/genética , Sequência de Bases , China , Feminino , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Humanos , Desequilíbrio de Ligação/genética , Masculino , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNARESUMO
OBJECTIVE: The purpose of this study was to develop a new method to regenerate articular cartilage of the temporomandibular joint (TMJ) by transplantation of free autogenous costal perichondrium (PC). STUDY DESIGN: In the study, 50 adult rabbits received the operation. For the surgery in the test group, the rabbits were randomly matched in pairs within the group and underwent the surgery of cross transplantation of costal PC after trimming the articular surface of the condyle. Operations were carried out in the same way in the experimental group except for PC transplantation. Instead, the suture was stitched in stratified order in the control group. Examination methods included observation of sections under the microscope, observation of specimens under the electron microscope, proliferating cell nuclear antigen (PCNA) staining, and biochemical analysis of glucuronic acid (GA) content and collagen content. The results of different groups were compared with ANOVA. RESULTS: The transplanted homologous PC escaped observable immune repulsion so that it could survive to form new joint cartilage with approximately normal tissue structure and biochemical constitution. The reproduction process was similar to the normal one, but was prolonged a little. At the later stage, the degeneration and calcification in the basal layer increased. However, because of its limited scope, no apparent effect on cartilage growth and function was observed. CONCLUSIONS: Homologous xenografting of free costal PC will not result in an apparent immunorejection of the host. Instead, the grafts can maintain their existence by obtaining nutrition from surrounding tissues and regenerate cartilage tissue.
Assuntos
Cartilagem/transplante , Côndilo Mandibular/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Articulação Temporomandibular/cirurgia , Animais , Cartilagem/patologia , Cartilagem Articular/cirurgia , Condrócitos/patologia , Colágeno/análise , Retículo Endoplasmático Rugoso/ultraestrutura , Ácido Glucurônico/análise , Hiperplasia , Côndilo Mandibular/patologia , Microscopia Eletrônica de Varredura , Microvilosidades/ultraestrutura , Antígeno Nuclear de Célula em Proliferação/análise , Proteoglicanas/análise , Coelhos , Distribuição Aleatória , Regeneração/fisiologia , Articulação Temporomandibular/patologia , Fatores de TempoRESUMO
OBJECTIVE: To measure the bone mineral density (BMD) of mandible and investigate the relationship between mandible and the whole body skeleton BMD. METHODS: Healthy volunteers were recruited in north China, which were divided into 6 groups by age: > or = 20, > or = 30, > or = 40, > or = 50, > or = 60 and > or = 70 years older, 10 male and 10 female in each group. Dual-energy X-ray absoptiometery (DXA) was used to measure the BMD of the lumbar spine, the mentum of mandible and the mandibular angle. The results were analyzed statistically. RESULTS: The mineral density (MD) of the mentum was (1.310 9 +/- 0.035 5) g/cm2, the left mandibular angle (1.048 9 +/- 0.013 7) g/cm2, the right mandibular angle (1.0547 +/- 0.014 1) g/cm2, the lumbar spine (L2-L4) (1.121 1 +/- 0.017 2) g/cm2. There was a significant difference in mandibular angle and lumbar MD between men and women (P < 0.05). The MD of mandibular angle and lumbar spine decreased significantly after 50 years of age. CONCLUSIONS: The normal BMD of the mandibular mentum, mandibular angles and lumbar spine is obtained. The BMD of the mandibular angles is closely related to that of the lumbar spine. Mandible can be an appropriate measurement site in the evaluation of skeleton BMD status for the forecast of osteoporosis.
Assuntos
Densidade Óssea , Mandíbula/anatomia & histologia , Adulto , Idoso , China , Feminino , Humanos , Masculino , Mandíbula/diagnóstico por imagem , Pessoa de Meia-Idade , Radiografia , Adulto JovemRESUMO
OBJECTIVE: To analyze the formant frequency of vowels in the sequence therapy of patient with cleft palate. METHODS: The formant frequency of vowels [a], [e], [i], [u] of normal children and postoperative patients with and without speech therapy was measured and analyzed by VS-99. RESULTS: 1. The mean value of F1, F2, F3 of [a] did not show significant difference among the three groups (P > 0.05). 2. The difference of mean value of [e] was significant between control group and pre-speech-therapy group, and between pre-speech-therapy and post-speech-therapy group (P < 0.05), but no significant difference was found between post-speech-therapy and control group(P > 0.05). The mean value of the formant in post-speech-therapy was higher than that of pre-speech-therapy. 3. The difference of mean value of [i] was significant between pre-speech-therapy and post-speech-therapy (P < 0.05), the mean value of F2, F3 in post-speech-therapy group decreased significantly compared with control (P < 0.05). 4. The difference of mean value of [u] showed significance between pre-speech-therapy and post-speech-therapy (P < 0.05), while the differences among other groups were insignificant (P > 0.05). CONCLUSION: Surgical repair of cleft palate cannot make all patients obtain perfect Velopharyngeal competence (VPC), while speech therapy can improve patient's pronunciation. Speech spectrum analysis can judge the effect of cleft palate therapy objectively.