Incidence and characteristic of deep venous thrombosis in hospitalized chronic heart failure patients.

BACKGROUND: This study was conducted to investigate the incidence of deep venous thrombosis (DVT), outcomes and its characteristics in patients with chronic heart failure (CHF) in a retrospective setting. OUTCOMES: Patients died of cardiac shock or acute exacerbation of heart failure (HF), admitted to intensive care unit (ICU) due to acute exacerbation of HF, patients decided to withdraw treatment and return home due to acute exacerbation of HF. METHODS: From January 2015 to June 2022, we admitted 359 patients diagnosed with CHF, and lower limb ultrasonography was performed for the examination of DVT after admission. The incidence of DVT was recorded and patients with known risk factors of VTE were identified and excluded after incidence of DVT was calculated. Patients' clinical data were then collected. RESULTS: The occurrence of DVT was 10.0% (36/359), as calf intramuscular vein thrombosis was the main constitution (n = 28, 75%). DVT patients with other factors (carcinoma, surgery, stroke, previous history of DVT) constituted a considerable part (33.3%, 12/36). Age, history of Diabetes Mellitus (DM), levels of DDi (D-Dimer), levels of alanine transferase (ALT) and left ventricular end-diastolic diameter (LVEDd) were independent predictors or risk factors of DVT in CHF patients, while chronic kidney disease (CKD) stage 1-4, white blood cell (WBC) and direct oral anticoagulant (DOAC) were protective factors. Incidence of DVT was correlated with a poor outcome of CHF patients (Pearson Chi-Square test, Value 19.612, P < 0.001). CONCLUSIONS: In this retrospective study, incidence of DVT was found to be relatively high among hospitalized CHF patients, while patients with DVT was associated with a poor prognosis.

Molecular engineering of PETase for efficient PET biodegradation.

The widespread utilization of polyethylene terephthalate (PET) has caused a variety of environmental and health problems. Compared with traditional thermomechanical or chemical PET cycling, the biodegradation of PET may offer a more feasible solution. Though the PETase from Ideonalla sakaiensis (IsPETase) displays interesting PET degrading performance under mild conditions; the relatively low thermal stability of IsPETase limits its practical application. In this study, enzyme-catalysed PET degradation was investigated with the promising IsPETase mutant HotPETase (HP). On this basis, a carbohydrate-binding module from Bacillus anthracis (BaCBM) was fused to the C-terminus of HP to construct the PETase mutant (HLCB) for increased PET degradation. Furthermore, to effectively improve PET accessibility and PET-degrading activity, the truncated outer membrane hybrid protein (FadL) was used to expose PETase and BaCBM on the surface of E. coli (BL21with) to develop regenerable whole-cell biocatalysts (D-HLCB). Results showed that, among the tested small-molecular weight ester compounds (p-nitrophenyl phosphate (pNPP), p-Nitrophenyl acetate (pNPA), 4-Nitrophenyl butyrate (pNPB)), PETase displayed the highest hydrolysing activity against pNPP. HP displayed the highest catalytic activity (1.94 µM(p-NP)/min) at 50 °C and increased longevity at 40 °C. The fused BaCBM could clearly improve the catalytic performance of PETase by increasing the optimal reaction temperature and improving the thermostability. When HLCB was used for PET degradation, the yield of monomeric products (255.7 µM) was ∼25.5 % greater than that obtained after 50 h of HP-catalysed PET degradation. Moreover, the highest yield of monomeric products from the D-HLCB-mediated system reached 1.03 mM. The whole-cell catalyst D-HLCB displayed good reusability and stability and could maintain more than 54.6 % of its initial activity for nine cycles. Finally, molecular docking simulations were utilized to investigate the binding mechanism and the reaction mechanism of HLCB, which may provide theoretical evidence to further increase the PET-degrading activities of PETases through rational design. The proposed strategy and developed variants show potential for achieving complete biodegradation of PET under mild conditions.

Fiber Bragg gratings inscribed in nanobore fibers.

The nanobore fiber (NBF) is a promising nanoscale optofluidic platform due to its long nanochannel and unique optical properties. However, so far, the applications of NBF have been based only on its original fiber geometry without any extra functionalities, in contrast with various telecom fiber devices, which may limit its wide applications. Here, we provide the first, to the best of our knowledge, demonstration of NBF-based fiber Bragg gratings (FBGs) introduced by either the femtosecond (fs) laser direct writing technique or the ultraviolet (UV) laser phase mask technique. Moreover, the FBG fabricated via the UV laser was optimized, achieving a high reflectivity of 96.89% and simultaneously preserving the open nanochannel. The NBF-based FBGs were characterized in terms of temperature variation and the infiltration of different liquids, and they showed high potential for nanofluidic applications.

Palladium-Catalyzed Intramolecular Heck/Aminocarbonylation of Alkene-Tethered Iodobenzenes with Nitro Compounds: Synthesis of Carbamoyl-Substituted Benzoheterocycles.

A palladium-catalyzed intramolecular Heck/aminocarbonylation of alkene-tethered iodobenzenes with nitro compounds has been developed for the synthesis of carbamoyl-substituted benzoheterocycles. Using Mo(CO)6 as a solid CO source, no external reductant or additives were needed in this procedure. Both nitroarenes and nitroalkanes were well tolerated. A range of carbamoyl-substituted dihydrobenzofurans and indolines were prepared in moderate to high yields.

High-precision optical frequency transfer over a 96 km urban fiber link.

We demonstrated the optical frequency transfer over a 96 km urban business network in Shanghai. The key factors affecting the optical frequency transmission system, such as fiber link quality, feedback compensation strength, and out-of-loop fiber temperature variation, are studied for the urban fiber link characteristics. The effective suppression technique of complex urban fiber link noise with different feedback compensation parameters is studied. With active phase noise suppression, the optical frequency stability can reach 1.9×10-16 at 1 s and 2.2×10-18 at 10,000 s over a 96 km urban fiber link. This work potentially plays an important role in optical clock frequency comparison, and it makes a good foundation for future research on long-distance optical frequency transfer over an urban business network.

Distributed fiber mountain seismic monitoring and steady-state analysis under natural earthquakes.

Mountain dynamic response monitoring plays important roles in geological disaster evolution monitoring and warning. A distributed mountain seismic monitoring and steady-state analysis method is demonstrated with distributed acoustic sensing (DAS) and a natural earthquake stimulus. In the field test, the seismic detection capability is first verified by comparing the recorded seismic waveforms from DAS and existing seismic stations. The vibration signal difference between steady-state and unsteady-state mountain parts is apparent; the operational modal analysis method is utilized to extract the response difference and to monitor the disaster evolution process. The proposed method has many advantages, including being easy to deploy, all-weather online monitoring, etc. It is believed that the proposed method will broaden the DAS application scope and promote the development of geological disaster early warning such as landslides and collapses.

An Investigation of All Fiber Free-Running Dual-Comb Spectroscopy.

A dual-comb spectroscopy (DCS) system uses two phase-locked optical frequency combs with a slight difference in the repetition frequency. The spectrum can be sampled in the optical frequency (OF) domain and reproduces the characteristics in the radio frequency (RF) domain through asynchronous optical sampling. Therefore, the DCS system shows great advantages in achieving precision spectral measurement. During application, the question of how to reserve the mutual coherence between the two combs is the key issue affecting the application of the DCS system. This paper focuses on a software algorithm used to realize the mutual coherence of the two combs. Therefore, a pair of free-running large anomalous dispersion fiber combs, with a center wavelength of approximately 1064 nm, was used. After the signal process, the absorption spectra of multiple species were simultaneously obtained (simulated using the reflective spectra of narrow-bandwidth fiber Bragg gratings, abbreviated as FBG). The signal-to-noise ratio (SNR) could reach 13.97 dB (25) during the 100 ms sampling time. In this study, the feasibility of the system was first verified through the simulation system; then, a principal demonstration experiment was successfully executed. The whole system was connected by the optical fiber without additional phase-locking equipment, showing promise as a potential solution for the low-cost and practical application of DCS systems.

Stable optical and radio frequency joint transfer based on a passive phase compensation.

We propose a novel scheme that uses only a single passive phase compensation device to achieve stable optical and radio frequency joint transfer. The phase noises of optical and radio frequency can be simultaneously compensated by passively embedding their phase information on the two optical carrier sidebands generated by an electro-optical modulator without using the phase discrimination and active servo controller. As a result, this scheme has many advantages, such as high spectral purity, short settling time and infinite compensation accuracy. We experimentally demonstrate the joint transfer of optical and 1 GHz RF over 120 km fiber spools. The optical frequency stability achieves 6.9 × 10-17 at 1 s and 7.03 × 10-19 at 10000 s, while the 1 GHz RF is 6.47 × 10-13 at 1 s and 3.96 × 10-16 at 10000 s.

Robust single-sideband-modulated Raman light generation for atom interferometry by FBG-based optical rectangular filtration.

Low-phase-noise and pure-spectrum Raman light is vital for high-precision atom interferometry by two-photon Raman transition. A preferred and prevalent solution for Raman light generation is electro-optic phase modulation. However, phase modulation inherently brings in double sidebands, resulting in residual sideband effects of multiple laser pairs beside Raman light in atom interferometry. Based on a well-designed rectangular fiber Bragg grating and a plain electro-optic modulator, optical single-sideband modulation has been realized at 1560 nm with a stable suppression ratio better than -25 dB despite of intense temperature variations. After optical filtration and frequency doubling, a robust phase-coherent Raman light at 780 nm is generated with a stable SNR of better than -19 dB and facilitates measuring the local gravity successfully. This FBG-based all-fiber single-sideband-modulated Raman light source, proposed for the first time and characterized as robust, compact and low-priced, is practical and potential for field applications of portable atom interferometry.

Fiber-optic time-frequency transfer in gigabit ethernet networks over urban fiber links.

We demonstrate a new optical pulse amplitude modulation (PAM) scheme where joint ultrastable time-frequency and gigabit ethernet data transfer with the same laser wavelength is realized. Time transmission is compatible with the White Rabbit (WR) based on gigabit ethernet networks, and frequency transmission is achieved by using 100MHz radio frequency (RF) modulation and the round-trip compensation methods. The laser is on-off keying (OOK) modulated by the WR signal, the RF and WR signal are modulated by optical PAM in a Mach-Zehnder interferometer modulator (MZM), and the local and remote site are connected by 96km urban fiber in Shanghai. The experimental results demonstrate that the frequency instabilities are 5.7E-14/1 s and 5.9E-17/104s, and the time interval transfer of 1 pulse per second (PPS) signal with less than 300fs stability after 104 s are obtained. This novel scheme can transmit frequency signals at hydrogen-maser-level stability in the gigabit ethernet network.

All-fiber narrow-bandwidth rectangular Optical filter with reconfigurable bandwidth and tunable center wavelength.

In this manuscript, a novel narrow-bandwidth rectangular optical filter based on multi-phase-shifted fiber Bragg grating (MPSFBG) is proposed. Using the local temperature control technology, the precise controllable phase shifts are introduced at different positions of the fiber Bragg grating (FBG). Therefore, the bandwidth of the MPSFBG-based filter with good shape factor can be reconfigured from 70 MHz to 1050 MHz by flexibly controlling the numbers and the positions of the phase shifts introduced in the MPSFBG. In addition, the center wavelength of the MPSFBG-based filter can be tuned through controlling the MPSFBG's environment temperature, and the tuning range of 22 GHz is realized. This is one of the best results for the narrow-bandwidth rectangular optical tunable filter with reconfigurable bandwidth. It can be widely used in the processing of reconfigurable signals in the optical communication networks and microwave photonics.

Aggressive hydration compared to standard hydration with lactated ringer's solution for prevention of post endoscopic retrograde cholangiopancreatography pancreatitis.

BACKGROUND AND AIMS: Previous studies have suggested that aggressive hydration with lactated ringer solution are one of the protective factors in preventing post endoscopic retrograde cholangiopancreatography (post-ERCP). We conducted a systematic review and meta-analysis to examine the efficacy aggressive hydration with lactated Ringer solution in preventing PEP. METHODS: All published and unpublished articles on aggressive hydration with lactated ringer solution in those underwent ERCP procedure for any reasons were screened for eligibility. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were followed. This paper doesn't need the IRB approval. RESULTS: Seven RCTs met the inclusion criteria. Meta-analysis indicates that aggressive hydration with lactated Ringer solution were associated with lower PEP rate.[odds ratio (OR) 0.29; 95% confidence interval (CI) 0.18-0.48]; lower incidence of hyperamylasemia (OR 0.49; 95% CI 0.35, 0.69) and lower risk of pain (OR 0.28; 95% CI 0.10-0.81). The association between aggressive hydration with lactated Ringer solution and incidence of moderate severity PEP were unclear (OR 0.57; 95% CI 0.22, 1.45). Sensitivity analyses also showed that omitting 1 study from analysis of PEP rate could reduce the heterogeneity but did not change the conclusion of this meta-analysis. A cumulating meta-analysis was performed statistically which showed a stable result of overall incidence of PEP. CONCLUSIONS: Aggressive hydration with lactated Ringer solution was a protective factor in reducing the overall incidence of PEP, hyperamylasemia and risk of abdominal pain.

High SNR Φ-OTDR with Multi-Transverse Modes Heterodyne Matched-Filtering Technology.

Phase-sensitive optical time domain reflectometer (Φ-OTDR) has attracted attention in scientific research and industry because of its distributed dynamic linear response to external disturbances. However, the signal-to-noise ratio (SNR) of Φ-OTDR is still a limited factor by the weak Rayleigh Backscattering coefficient. Here, the multi-transverse modes heterodyne matched-filtering technology is proposed to improve the system SNR. The capture efficiency and nonlinear threshold are increased with multiple transverse modes in few-mode fibers; the incident light energy is permitted to be enlarged by a wider probe pulse by using heterodyne matched-filtering without spatial resolution being deteriorated. As far as we know, this is the first time that both multi-transverse modes integration method and digital heterodyne matched filtering method have been used to improve the SNR of Φ-OTDR simultaneously. Experimental results show that the noise floor is reduced by 11.4 dB, while the target signal is kept. We believe that this proposed method will help DAS find important applications in marine acoustic detection and seismic detection.

Ultralow noise DFB fiber laser with self-feedback mechanics utilizing the inherent photothermal effect.

Single frequency laser sources with low frequency noise are now at the heart of precision high-end science, from the most precise optical atomic clocks to gravitational-wave detection, thanks to the rapid development of laser frequency stabilization techniques based on optical or electrical feedback from an external reference cavity. Despite the tremendous progress, these laser systems are relatively high in terms of complexity and cost, essentially suitable for the laboratory environment. Nevertheless, more and more commercial applications also demand laser sources with low noise to upgrade their performance, such as fiber optic sensing and LiDAR, which require reduced complexity and good robustness to environmental perturbations. Here, we describe an ultralow noise DFB fiber laser with self-feedback mechanics that utilizes the inherent photothermal effect through the regulation of the thermal expansion coefficient of laser cavity. Over 20 dB of frequency noise reduction below several tens of kilohertz Fourier frequency is achieved, limited by the fundamental thermal noise, which is, to date, one of the best results for a free-running DFB fiber laser. The outcome of this work offers promising prospects for versatile applications due to its ultralow frequency noise, simplicity, low cost, and environmental robustness.

Multi-source aliasing suppression for distributed fiber acoustic sensing with directionally coherent enhancement technology.

Distributed fiber acoustic sensing (DAS) can detect almost all disturbances along the sensing fiber and is widely applied. However, the signals from multiple adjacent disturbance sources are superimposed, according to the sensing principle. A directionally coherent enhancement technology is demonstrated for DAS to suppress multi-source aliasing in air. In preliminary works, two situations are considered for feasibility verification. The submerged weak target signal is effectively extracted from strong broadband noise, and two different same-frequency signals from two sources are separately rebuilt with the same detected signal. As far as we know, this is the first time that the directionally coherent enhancement is proposed for DAS and the multi-source aliasing is suppressed. This technique will help DAS find new important foreground in acoustic detection of large-scale plants with many similar noisy devices, including discharge detection in high voltage substations and acoustic emission flaw detection in mechanical factories.

Non-coding RNAS and colorectal cancer liver metastasis.

More than 50% of colorectal cancer (CRC) deaths are attributed to metastasis, and the liver is the most common distant metastatic site of CRC. The molecular mechanisms underlying CRC liver metastasis are very complicated and remain largely unknown. Accumulated evidence has shown that non-coding RNAs (NcRNAs) play critical roles in tumor development and progression. Here we reviewed the roles and underlying mechanisms of NcRNAs in CRC liver metastasis.

Narrow linewidth swept laser source based on cascaded multi-wavelength injection of DFB lasers.

A linearly swept laser source over broadband with a fast sweep rate and narrow linewidth is realized using a novel optoelectronic scheme based on a multi-wavelengths (mutually coherent) injected distributed feedback (DFB) laser. Under the condition of multi-wavelengths injection, the injection-locking and four-wave mixing (FWM) process can occur simultaneously in the DFB laser, inducing a swept laser source with a sweep range of 100 GHz and sweep rate of 10 THz/s. Furthermore, with the phase noise character analyzation of the swept laser source, the phase noise deterioration due to the radio frequency (RF) signal is studied quantitatively. Besides the influence of the RF signal noise, the phase noise deterioration in the FWM process can be suppressed completely with the phase-locked pump beam and signal beam based on the injection-locking principle. This low phase noise swept laser source with sub-kilohertz linewidth could have wide applications in lidar.

PIK3CD induces cell growth and invasion by activating AKT/GSK-3ß/ß-catenin signaling in colorectal cancer.

The catalytic subunit p110δ of phosphoinositide 3-kinase (PI3K) encoded by PIK3CD has been implicated in some human solid tumors. However, its roles in colorectal cancer (CRC) remain largely unknown. Here we found that PIK3CD was overexpressed in colon cancer tissues and CRC cell lines and was an independent predictor for overall survival (OS) of patients with colon cancer. The ectopic overexpression of PIK3CD significantly promoted CRC cell growth, migration and invasion in vitro and tumor growth in vivo. In contrast, inhibition of PIK3CD by specific small-interfering RNA or idelalisib dramatically suppressed CRC cell growth, migration and invasion in vitro and tumor growth in vivo. Moreover, PIK3CD overexpression increased AKT activity, nuclear translocation of ß-catenin and T-cell factor/lymphoid enhancer factor (TCF/LEF) transcriptional activity and decreased glycogen synthase kinase 3ß (GSK-3ß) activity, whereas PIK3CD inhibition exhibited the opposite effects. Furthermore, PIK3CD-mediated cell growth, migration and invasion were reversed by blockade of AKT signaling or depletion of ß-catenin. In addition, PIK3CD expression in colon cancer tissues positively correlated with ß-catenin abnormal expression, which was an independent predictor for OS of colon cancer patients. Taken together, our findings demonstrate that PIK3CD is an independent prognostic factor in CRC and that PIK3CD induces CRC cell growth, migration and invasion by activating AKT/GSK-3ß/ß-catenin signaling, suggesting that PIK3CD might be a novel prognostic biomarker and a potential therapeutic target for CRC.

Intensity noise in high-frequency range of an external cavity diode laser and its reduction by second harmonic generation.

Characteristics of intensity noise of optically pumped vertical-cavity surface-emitting lasers and solid-state lasers, induced by the beating of the main lasing mode and non-lasing side modes and its reduction, have been reported in recent years. The mode beating noise of an external cavity diode laser composed of an electrically pumped edge-emitting laser diode chip is studied experimentally in this Letter. The noises due to the beating of the main mode with the first- to third-order side modes are observed, and multiple sub-peaks in the beating noise are measured. It is assumed that the new phenomena are coming from the enhanced four-wave mixing in the longer-active medium. Intensity noise reduction is also demonstrated by using the second harmonic generation of a beta barium borate crystal as a nonlinear absorber.

Photothermal effects in phase shifted FBG with varied light wavelength and intensity.

The intensity enhancement effect of a phase-shifted fiber Bragg grating (PSFBG) is investigated theoretically and experimentally in this paper. Due to the effect, both of the FBG reflection bands and the transmission peak show red-shift with the increase of pump light wavelength from the shorter side to the longer side of the Bragg wavelength. The transmission peak shifts in pace with the pump's wavelength, which is much faster than the reflection band. The maximum shift increases with the pump power. In contrast, the red-shift is very small when the pump light deceases from the longer side of the Bragg wavelength. Such asymmetric behavior is checked dynamically by using a frequency modulated laser in a serrated wave, showing push-pull behavior. The effect of the characteristics of thermal dissipation conditions is also measured. The fiber loss coefficient of FBG being tested is estimated from the measured data to be about 0.001 mm-1, which may be attributed to the H2-loading and UV exposure in FBG fabrication. The observed phenomena are believed of importance in application where PSFBG is utilized as a narrow linewidth filter.