Transcriptional regulation of the vitellogenin gene through a fecundity-related single nucleotide polymorphism within a GATA-1 binding motif in the brown planthopper, Nilaparvata lugens.

Identifying the Single Nucleotide Polymorphisms (SNPs) with functions in insect fecundity promises to provide novel insight into genetic mechanisms of adaptation and to aid in effective control of insect populations. We previously identified several SNPs within the vitellogenin (Vg) promoter region between a high-fecundity population (HFP) and a low-fecundity population (LFP) of the brown planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae). Here, we found that an A-to-T (HFP allele to LFP allele) transversion at nucleotide -953 upstream of Vg in a Nilaparvata lugens GATA-1 (NlGATA-1) binding motif is associated with the level of Vg transcription. We also characterized NlGATA-1, containing a double CX2 CX17 CX2 C zinc finger, which has been implicated in the activation of Vg gene expression. Knockdown of the NlGATA-1 gene results in a reduced basal level of expression of the Vg gene and fewer offspring of N. lugens in vivo, whereas overexpression of NlGATA-1 in cells increased Vg promoter activity. Moreover, upon cotransfection with NlGATA-1 expression vector, the luciferase activities of Vg reporter vectors with the A allele were significantly higher than those with the T allele. These findings support a mechanism in which a SNP within the promoter of Vg is associated with the level of Vg transcription by altering the binding activity of NlGATA-1 and subsequently affecting fecundity in N. lugens.

Intra- and inter-specific variation in alarm pheromone produced by Solenopsis fire ants.

Some fire ants of the genus Solenopsis have become invasive species in the southern United States displacing native species by competition. Although the displacement pattern seems clear, the mechanisms underlying competitive advantage remain unclear. The ability of ant workers to produce relatively larger amount of alarm pheromone may correspond to relative greater fitness among sympatric fire ant species. Here we report on quantitative intra-specific (i.e. inter-caste) and inter-specific differences of alarm pheromone component, 2-ethyl-3,6-dimethylpyrazine (2E36DMP), for several fire ant species. The alarm pheromone component was extracted by soaking ants in hexane for 48 h and subsequently quantified by gas chromatography-mass spectrometry at single ion monitoring mode. Solenopsis invicta workers had more 2E36DMP than male or female alates by relative weight; individual workers, however, contained significantly less pyrazine. We thus believe that alarm pheromones may serve additional roles in alates. Workers of Solenopsis richteri, S. invicta, and hybrid (S. richteri × S. invicta) had significantly more 2E36DMP than a native fire ant species, Solenopsis geminata. The hybrid fire ant had significantly less 2E36DMP than the two parent species, S. richteri and S. invicta. It seems likely that higher alarm pheromone content may have favored invasion success of exotic fire ants over native species. We discuss the potential role of inter-specific variation in pyrazine content for the relationship between the observed shifts in the spatial distributions of the three exotic fire ant species in southern United States and the displacement of native fire ant species.

Crustal Structure of Yunnan Province, People's Republic of China, from Seismic Refraction Profiles.

Seismic refraction, profiles in Yunnan Province, southwestern China, define the crustal structure in an area of active tectonics on the southern end of the Himalaya-Burma arc. The crustal thickness ranges from 38 to 46 kilometers, and the relatively low mean crustal velocity indicates a crustal composition compatible with normal continental crust and consisting mainly of meta-sedimentary and silicic intrusive rocks, with little mafic or ultramafic component. This composition suggests a crustal evolution involving sedimentary processes on the flank of the Yangtze platform rather than the accretion of oceanic island arcs, as has been proposed. An anomalously low upper-mantle velocity observed on one profile but not on another at right angles to it may indicate active tectonic processes in the mantle or seismic anisotropy.

[The use of coral as a substitute for maxillofacial bone reconstruction].

A kind of coral, Porites, which was originated from the Madreporaria reef builders in Hainan Province, was studied as a bone substitute. It is almost composed of aragonite (CaCO(s)) and the structure shows interconnected micropores with a-mean diameter of 150-200 microns which is suitable for bone ingrowth. The coral was implanted successfully into the defects in inferior border of mandibles of 8 rabbits for 6 months. Then it was used clinically as a bone substitute in maxillofacial surgery for 22 patients who were followed up for 6-24 months. With X-ray, histological and clinical examination, the results showed that the coral had a good tolerance and elicited no deleterious host response. After implanted, the coral is acted as a bone conductor and can promote the bone repair of defects. It seems to be a useful bone substitute.