FBXO22 inhibits proliferation and metastasis of cervical cancer cells by mediating ubiquitination-dependent degradation of GAK.

Cervical cancer is one of the recognized malignant tumors of female reproductive system. At present, the research and development of biomarkers has attracted increasing attention, and the wide application of clinical cervical cancer screening strategies has significantly reduced its morbidity and mortality. A member of the F-box protein family, FBXO22, is involved in cell cycle, DNA damage repair and many other processes. Dysregulation of FBXO22 plays an important role in the occurrence and development of various tumors, including ovarian cancer, liver cancer and lung cancer. Nevertheless, the effect of FBXO22 in cervical cancer needs further investigation. We found that FBXO22 inhibited cervical cancer cell proliferation, migration and invasion. The results of proteomics studies suggested FBXO22 appears to target the Cyclin G Associated Kinase (GAK) for degradation. The combined results of analysis of cultured cells with altered abundance of FBXO22 by depletion or over-expression in the presence or absence of proteasomal inhibitor, comparison of protein decay rate, as well as cellular ubiquitination, support a hypothesis that FBXO22 mediates the ubiquitin-dependent degradation of GAK. Taken together, our data suggest that FBXO22 has a protective role in cervical cancer.

Multi-stage aeration regime to regulate organic conversion toward gas alleviation and humification in food waste digestate composting.

Aerobic composting has been considered as a pragmatic technique to convert food waste digestate into high-quality biofertiliser. Nevertheless, massive gaseous emission and immature product remain the primary challenges in food waste digestate composting. Thus, the performance of multi-stage aeration regimes to improve gaseous emissions and organic humification during food waste digestate composting was investigated in this study. In addition to continuous aeration with a constant intensity of 0.3 L kg·dry mass (DM)-1·min-1, two multi-stage decreased aeration regimes were designed as "0.3-0.2-0.1" and "0.3-0.1-0.1" L·kg·DM-1·min-1 from the thermophilic to cooling and then mature stages, respectively. Results showed that the decreased aeration regimes could alleviate nitrous oxide (N2O) and ammonia (NH3) emission and slightly enhance humification during composting. The alleviated N2O and NH3 emission were mainly contributed by abiotically reducing gaseous release potential as well as biotically inactivating denitrifers (Pusillimonas and Pseudidiomarina) and proliferating Atopobium to reduce nitrate availability under lower aeration supply. The "0.3-0.2-0.1 L kg·DM-1·min-1" regime exhibited a more excellent performance to alleviate N2O and NH3 emission by 27.5% and 16.3%, respectively. Moreover, the decreased aeration regimes also favored the enrichment of functional bacteria (Caldicoprobacter and Syntrophomonas) to accelerate lignocellulosic biodegradation and thus humic acid synthesis by 6.5%-11.2%. Given its better performance to improve gaseous emissions and humification, the aeration regime of "0.3-0.2-0.1 L kg·DM-1·min-1" are recommended in food waste digestate composting in practice.

Effect of moisture content, aeration rate, and C/N on maturity and gaseous emissions during kitchen waste rapid composting.

To determine factors affecting compost maturity and gaseous emissions during the rapid composting of kitchen waste, an orthogonal test was conducted with three factors: moisture content (MC) (55%, 60%, 65%), aeration rate (AR) (0.3,0.6 and 0.9 L·kg-1DM·min-1) and C/N ratio (21, 24, 27). The results showed that the importance of factors affecting compost maturity was: C/N > AR > MC, optimal conditions were: C/N of 24, AR of 0.3 L·kg-1DM·min-1and MC of 65%. For gaseous emissions, the sequence of essential factors affecting NH3 emissions was: C/N > MC > AR, and the optimal parameters for NH3 reduction were: C/N of 27, MC of 65%, and AR of L·kg-1DM·min-1. The important factors affecting N2O and H2S emissions are both: MC > C/N > AR, while their best parameters were different. The optimal parameters for N2O emission reduction were MC of 60%, AR of 0.3 L·kg-1DM·min-1 and C/N of 24, while these for H2S were MC of 55%, AR of 0.3 L·kg-1DM·min-1 and C/N of 21. The C/N mainly affected the compost maturity and AR further affected the maturity and pollutant gas emissions by influencing the temperature and O2 content. Considering comprehensively the maturity and gaseous reduction, the optimal control parameters were: MC of 60%-65%, AR of L·kg-1DM·min-1, and C/N of 24-27.

Family intergenerational stress: Concept exploration and development via coping and identity management.

The sandwich generation faces dual responsibilities of supporting parents and raising children, resulting in heightened levels of stress and negative work-related outcomes. Despite a wealth of research on the sandwich generation, few studies have examined the specific nature of the multigenerational needs of the sandwich generation. Accordingly, we introduce a new concept termed family intergenerational stress (FIS), which refers to the interaction and integration of stresses from elder caregiving, child-rearing, and associated challenges with self-definition. Through the lens of FIS, we collected interview data from 137 sandwich generation employees, secondary network data, and field observation data from 21 employees. We further used grounded theory to explore employees' stress and coping responses to dual family responsibilities. Findings indicate that employees with both elder caregiving and child-rearing responsibilities experience FIS. This, in turn, threatens their identity as an ideal worker and is associated with lower work engagement. Findings further reveal that in coping with FIS, employees adopt different identity-based strategies. Theoretical and practical implications are discussed. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Plant-derived biochar amendment for compost maturity improvement and gaseous emission reduction in food waste composting: Insight from bacterial community and functions.

This study assessed the impact of different plant-derived biochar (cornstalk, rice husk, and sawdust) on bacterial community and functions for compost maturity and gaseous emissions during the composting of food waste. Results showed that all biochar strengthened organic biotransformation and caused a higher germination index on day 12 (over 100%), especially for rice husk biochar to enhance the growth of Thermobifida related to aerobic chemoheterotrophy. Rice husk biochar also achieved a relatively higher reduction efficiency of methane (85.8%) and ammonia (82.7%) emissions since its greater porous structure. Besides, the growth of Pseudomonas, Pusillimonas, and Desulfitibacter was restricted to constrict nitrate reduction, nitrite respiration, and sulfate respiration by optimized temperature and air permeability, thus reducing nitrous oxide and hydrogen sulfide emissions by 48.0-57.3% by biochar addition. Therefore, rice husk biochar experienced the optimal potential for maturity increment and gaseous emissions mitigation.

Optimization of free air space to regulate bacterial succession and functions for alleviating gaseous emissions during kitchen waste composting.

This study investigated the effects of free air space (FAS) (45%, 55%, 65%) on bacterial dynamics for gaseous emissions during kitchen waste composting. Results show that FAS increase from 45% to 65% elevated oxygen diffusivity to inhibit bacteria for fermentation (e.g. Caldicoprobacter and Ruminofilibacter) to reduce methane emission by 51%. Moreover, the increased FAS accelerated heat loss to reduce temperature and the abundance of thermophiles (e.g. Thermobifida and Thermobacillus) for aerobic chemoheterotrophy to mitigate ammonia emission by 32%. Nevertheless, the reduced temperature induced the growth of Desulfitibacter and Desulfobulbus for sulfate/sulfite respiration to boost hydrogen sulphide emission. By contrast, FAS at 55% achieved the highest germination index and favored the proliferation of nitrifiers and denitrifiers (e.g. Roseiflexus and Steroidobacter) to improve nitrate availability, thus slightly enhancing nitrous oxide emission. Thus, FAS at 55% exhibits the optimal performance for gaseous emission reduction and maturity enhancement in kitchen waste composting.

Regulating aeration intensity to simultaneously improve humification and mitigate gaseous emissions in food waste digestate composting: Performance and bacterial dynamics.

This study assessed the impact of aeration intensity on food waste digestate composting to simultaneously govern organic humification and gaseous emissions. Results show that an augment in the aeration intensity from 0.1 to 0.4 L·kg-1 DM·min-1 provided more oxygen to facilitate organic consumption and thus temperature increase, but slightly restrained organic humification (e.g. less humus content and higher E4/E6 ratio) and substrate maturity (i.e. lower germination index). Furthermore, increasing aeration intensity inhibited the proliferation of the genera Tepidimicrobium and Caldicoprobacter to alleviate methane emission and enriched the genus Atopobium to boost hydrogen sulphide production. More importantly, increasing aeration intensity limited the growth of the genus Acinetobacter for nitrite/nitrogen respiration, but strengthened aerodynamics to blow out nitrous oxide and ammonia produced inside piles. Principal component analysis comprehensively indicated that a low aeration intensity of 0.1 L·kg-1DM·min-1 facilitated precursors synthesis toward humus and simultaneously mitigated gaseous emissions to improve food waste digestate composting.

Post biopsy delayed liver hemorrhage successfully controlled by bedside CEUS-guided microwave ablation: A case report.

We present a 60-year-old man suffering from delayed arterial hemorrhage post liver biopsy. Contrast-enhanced ultrasound was used to detect the bleeding point and to evaluate the efficacy of microwave ablation (MWA). The hemorrhage was controlled by MWA at the bedside. This is the first application of MWA for delayed hemorrhage.

FI-CEUS: a solution to improve the diagnostic accuracy in MRI LI-RADS-indeterminate (LR-3/4) FLLs at risk for HCC.

Objective: To evaluate the diagnostic accuracy of fusion imaging contrast-enhanced ultrasound (FI-CEUS) of magnetic resonance imaging (MRI) LI-RADS-indeterminate (LR-3/4) and conventional ultrasound undetected focal liver lesions (FLLs) in patients at risk for hepatocellular carcinoma (HCC). Methods: Between February 2020 and July 2021, 71 FLLs in 63 patients were registered for diagnostic performance evaluation respectively for ultrasound-guided thermal ablation evaluation in this retrospective study. Diagnostic performance regarding FLLs was compared between FI-CEUS and contrast-enhanced MRI (CE-MRI). Results: For diagnostic performance evaluation, among 71 lesions in 63 patients, the diagnostic efficacy of FI-CEUS with LI-RADS was significantly higher than that of CE-MRI (P < 0.05) in both overall and hierarchical comparison (except for the group with lesion diameter ≥2 cm). For malignant lesions, the proportion of arterial phase hyperenhancement (APHE) and washout on FI-CEUS was higher than that on CE-MRI (P < 0.05). Conclusion: FI-CEUS has a high value in the precise qualitative diagnosis of small FLLs (<2 cm) of MRI LI-RADS-indeterminate diagnosis (LR-3/4) that are undetected by conventional ultrasound in patients at risk for HCC and can be a good supplementary CE-MRI diagnostic method for thermal ablation evaluation.

Work-Family Conflict and Unethical Pro-family Behavior: The Mediating Effect of Threat Appraisal and the Moderating Effect of Family Collectivism Orientation.

Unethical pro-family behavior (UPFB) is prevalent in organizations and has adverse effects on organizations, but very few studies have examined the factors that lead to UPFB. We use a cognitive appraisal theoretical framework to argue that employees' unethical pro-family (UPFB) behavior results from work and family conflicts (WFC/FWC) are mediated by threat appraisal and moderated family collectivism orientation. Based on the questionnaire data of 496 full-time employees from two-time points, we found that WFC/FWC was positively correlated with UPFB where threat appraisal played a mediating role in this relationship; Family collectivism orientation strengthens the threat appraisal-UPFB relationship and the mediation relationship between WFC/FWC and UPFB via threat appraisal. These findings offer an understanding of the theoretical and practical implications which could help organizations reduce UPFB. Finally, we discuss possible directions for future research.

Bacterial dynamics for gaseous emission and humification in bio-augmented composting of kitchen waste.

Using high-throughput sequencing and Functional Annotation of Prokaryotic Taxa (FAPROTAX), this study aimed to elucidate the effect of bacterial dynamics on gaseous emission and humification of kitchen and garden wastes during composting augmented with microbial inoculants. Microbial inoculant addition at up to 0.9% resulted in a diverse bacterial community with more functional bacteria to amend gaseous emission and enhance humification. Microbial inoculation facilitated the enrichment of aerobic bacteria (e.g. the genus Bacillus and Thermobifida) to enhance cellulolysis and ligninolysis to advance organic humification. By contrast, several bacteria, such as the genus Weissella and Pusillimonas were inhibited by microbial inoculation to weaken fermentation and nitrate respiration. As such, bio-augmented composting with 0.9% microbial inoculant reduced the emission of methane by 11-20% and nitrogen oxide by 17-54%. On the other hand, ammonia and hydrogen sulphide emissions increased by 26-62% and 5-23%, respectively, in bio-augmented composting due to the considerable proliferation of the genus Bacillus and Desulfitibacter to enhance ammonification and sulphur-related respiration. Results from this study highlight the need to further develop efficient and multifunctional microbial inoculants that promote humification and deodorization for bio-augmented composting of kitchen waste as well as other carbon and nutrient rich organic wastes.

Regulating bacterial dynamics by lime addition to enhance kitchen waste composting.

This study examined bacterial dynamics in response to lime addition to enhance kitchen waste composting using modular network analysis. Bacterial communities could be separated into three meta-modules corresponding to the mesophilic, thermophilic, and mature stage of composting. Lime addition at 1% (wet weight) suppressed acidogens and denitrifiers (e.g. Lactobacillus and Acinetobacter) at the mesophilic stage to reduce greenhouse gas emissions. The matrix pH and temperature were also increased by lime addition via hydrogen reaction to favor bacterial growth and activity. Thus, thermophilic bacteria (e.g. Thermoactinomycetaceae and Planifilum) were enriched with lime addition to facilitate lignocellulose biodegradation for humus formation at the thermophilic stage. Further lime addition to 1.5% reduced ammonia emission at the thermophilic stage via chemical fixation. Moreover, lime inhibited denitrifiers but proliferated nitrifiers at the mature stage to decrease nitrous oxide emission and enhance nitrate content, respectively. As such, lime addition improved both biotic and abiotic composting performance.

Bacterial dynamics and functions for gaseous emissions and humification in response to aeration intensities during kitchen waste composting.

This study revealed bacteria dynamics and functions for gaseous emissions and humification during kitchen waste composting under different aeration intensities (i.e. 0.24, 0.36, and 0.48 L kg-1 DM min-1) using high-throughput sequencing with Functional Annotation of Prokaryotic Taxa. Results show that aeration increase restrained bacteria (e.g. Lactobacillus and Acinetobacter) for fermentation, nitrate reduction, and sulphur/sulphate respiration, but enriched thermophilic bacteria (e.g. Thermomonospora and Thermobifida) for aerobic chemohetertrophy, xylanolysis, cellulolysis, and methylotrophy. Thus, high aeration intensity (i.e. above 0.36 L kg-1 DM min-1) effectively alleviated the emission of greenhouse gases and hydrogen sulphide, and meanwhile facilitated the production of humus precursors and ammonia. Nevertheless, humification was limited by the conclusion of composting under high aeration conditions due to the consumption of humus precursors for bacterial activity. Thus, aeration intensity should be regulated at different stages indicated by temperature to balance gaseous emissions and humification during kitchen waste composting.

Relating bacterial dynamics and functions to gaseous emissions during composting of kitchen and garden wastes.

This study aims to relate bacterial dynamics to gaseous emissions during the composting of kitchen and garden wastes. High-throughput sequencing and Functional Annotation of Prokaryotic Taxa (FAPROTAX) were used to analyse the bacterial community and potential functions during composting, respectively. Results show that the addition of garden waste up to 15% of the total wet weight of composting materials notably mitigated gaseous emissions and improved maturity during kitchen waste composting. Ammonium nitrogen, temperature, oxygen content, and electrical conductivity were identified as critical factors to impact gaseous emissions. The bacterial community analysis indicated that the proliferation of anaerobes during the storage of kitchen waste induced the dramatic emission of methane (CH4) and nitrous oxide (N2O) at the beginning of composting. Adding garden waste could effectively amend the physiochemical properties of composting materials to reduce the relative abundance of microbes (e.g. Desulfotomaculum and Caldicoprobacter) that contributed to gaseous emissions, but enrich those (e.g. Bacillus and Pseudoxanthomonas) for organic biodegradation. Further analysis by FAPROTAX corroborated that adding garden waste could effectively inhibit relevant microbial metabolisms (e.g. fermentation, nitrite/nitrate respiration and sulphate respiration) and thus alleviate the emission of greenhouse gases and odours during kitchen waste composting.

Bacterial dynamics and functions driven by bulking agents to mitigate gaseous emissions in kitchen waste composting.

This study investigated the impacts of different bulking agents (i.e. garden waste, cornstalks, and spent mushroom substrates) on bacterial structure and functions for gaseous emissions during kitchen waste composting. High-throughput sequencing was integrated with functional Annotation of Prokaryotic Taxa (FAPROTAX) to decipher the bacterial structure and functions. Results show that adding cornstalks constructed a more complex and mutualistic bacterial network to enhance organic biodegradation. This scenario, however, aggravated the emission of ammonia and hydrogen sulphide with the enrichment of the genus Bacillus and Desulfitibacter at the thermophilic stage of composting to facilitate ammonification and sulphur-related respiration, respectively. By contrast, spent mushroom substrates facilitated the proliferation of the genus Pseudomonas to promote nitrate reduction at the cooling stage, leading to considerable emission of nitrous oxide. Compared to these two agents, garden waste contained less easily biodegradable substances to limit bacterial mutualism, thereby reducing gaseous emissions in composting.