Biogas slurry recirculation regulates food waste fermentation: Effects and mechanisms.

Regularly adding biogas slurry into fermentation reactors is an effective way to enhance hydrogen or methane production. However, how this method affects the production of valuable organic acids and alcohols is still being determined. This study investigated the effects of different addition ratios on semi-continuous fermentation reactors using food waste as a substrate. The results showed that an addition ratio of 0.2 increased lactic acid production by 30% with a yield of 0.38 ± 0.01 g/g VS, while a ratio of 0.4 resulted in mixed acid fermentation dominated by n-butyric acid (0.07 ± 0.01 g/g VS) and n-caproic acid (0.06 ± 0.00 g/g VS). The introduction of Bifidobacteriaceae by biogas slurry played a crucial role in increasing lactic acid production. In contrast, exclusive medium-chain fatty acid producers enhanced the synthesis of caproic acid and heptanoic acid via the reverse ß-oxidation pathway. Mechanism analyses suggested that microbial community structure and activity, substrate hydrolysis, and cell membrane transport system and structure changed to varying degrees after adding biogas slurry.

Material flow analysis of an upgraded anaerobic digestion treatment plant with separated utilization of carbon and nitrogen of food waste.

Anaerobic digestion of food waste can recover carbon in the form of biogas, while the high concentration of ammonia nitrogen in the digestion effluent becomes troublesome. Therefore, some new treatment plants use three-phase centrifugation to separate homogenized food waste into nitrogen-rich fine slag for insect cultivation and carbon-rich liquid for anaerobic digestion. To analyze the effects of the carbon-nitrogen separation, an upgraded plant's material and elementary flows were investigated. The three-phase separation process redistributed carbon and nitrogen, and the biogas slurry was the primary output. The principal endpoint for C was the crude oil, capturing 57.1 ± 13.1 % of the total input; the find slag collected 48.3 ± 6.9 % of the total N input, and the biogas slag accepted 52.9 ± 4.4 % of the P input. The carbon-nitrogen separation strategy can improve digestion efficiency and increase treatment benefits significantly, marking a promising direction for future developments in food waste utilization.

Characterizing molecular transformation of dissolved organic matter during high-solid anaerobic digestion of dewatered sludge using ESI FT-ICR MS.

In this study, the effects of anaerobic digestion (AD) on molecular characteristics of dissolved organic matter (DOM) in the dewatered sludge has been described by advanced electrospray ionization combined with Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS) technology. With the progress of AD, molecular amounts in DOM samples increased with the lowering in the carbon atom number of average molecular formula and average double bond equivalent (DBE). CHON and CHONS groups are the two main organic substances in sludge with their relative DOM proportions of 29.64% and 32.56%, respectively. The resistants (i.e., refractory organic matter) mainly consist of the proteins regions of CHO groups as well as the proteins/lignin regions of CHON groups. The contrasting temporal trends in protein contents (e.g., decrease (CHO and CHON) vs. increase (CHONS)) may imply differences in their degradation characteristics. Likewise, the multi-N (N3, N4) and S2 organic groups in the sludge are converted to N2 and S1 molecules, while the relative abundance of O atoms (in Ox molecules) tends to increase. In addition, the resistants in sludge DOM contain high oxidizing C and low unsaturation. The overall results of this research are expected to provide the theoretical basis for further optimization of the sludge AD process.