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1.
Molecules ; 27(5)2022 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-35268762

RESUMO

Plants and fungi are known as a valuable source of natural medicines used in the treatment of various diseases. Many of them are used to treat human and animal gastrointestinal diseases caused by parasites. The aim of this study was to investigate for the first time the antinematode properties of extracellular low-molecular subfractions (ex-LMS) obtained from the liquid growth medium of idiophasic Cerrena unicolor cultures. The fungal fractions were isolated according to a procedure previously described by Jaszek et al. The in vitro tests were performed using nematodes of the Rhabditis genus. As demonstrated by the results, the total fraction with a molecular weight < 10 kDa (CU-A) and the 0.02−1.5 kDa fraction (CU-B) had nematicidal activity. It was found that the analyzed substances induced movement disturbances caused by the paralysis of the back part of the nematode's body. The degree of body paralysis was proportional to the increase in the concentration of the tested fractions. Summarizing the obtained results in the context of the available literature data, it seems that C. unicolor may be a good new candidate for research on nematode infections.


Assuntos
Polyporales , Rhabditoidea , Animais , Fungos , Peso Molecular
2.
Molecules ; 24(9)2019 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-31083598

RESUMO

BACKGROUND: The insensitivity of cancer cells to therapeutic agents is considered to be the main cause of failure of therapy and mortality of patients with cancer. A particularly important problem in these patients is the phenomenon of multidrug resistance, consisting of abnormal, elevated expression of transport proteins (ABC family). The aim of this research included determination of IC50 values of selected furanocoumarins in the presence and absence of mitoxantrone in leukemia cells and analysis of changes in apoptosis using anexinV/IP and Casp3/IP after 24 h exposure of cell lines to selected coumarins in the presence and absence of mitoxantrone in IC50 concentrations. METHODS: Research was conducted on 3 cell lines derived from the human hematopoietic system: HL-60, HL-60/MX1 and HL-60/MX2. After exposure to coumarin compounds, cells were subjected to cytometric analysis to determine the induction of apoptosis by two methods: the Annexin V test with propidium iodide and the PhiPhiLux-G1D2 reagent containing caspase 3 antibodies. RESULTS: All of the furanocoumarin derivatives studied were found to induce apoptosis in leukemia cell lines. CONCLUSIONS: Our results clearly show that the furanocoumarin derivatives are therapeutic substances with antitumor activity inducing apoptosis in human leukemia cells with phenotypes of resistance.


Assuntos
Furocumarinas/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Resistência a Múltiplos Medicamentos , Resistencia a Medicamentos Antineoplásicos , Citometria de Fluxo , Furocumarinas/química , Células HL-60 , Humanos , Leucemia Promielocítica Aguda/metabolismo
3.
Pathogens ; 12(2)2023 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-36839467

RESUMO

We present the case of a 60-year-old man who was diagnosed with a subcutaneous nodule on the upper eyelid of his left eye. The patient reported multiple mosquito bites during numerous work trips to Ukraine. Histopathological examination of the nodule isolated during surgery suggested Dirofilaria repens infestation. The infection was brought to Poland from the territory of Ukraine. Ophthalmologists must be aware of uncommon presentations of parasitic infestations when they consider infections of the ocular adnexa.

4.
Cell Mol Biol Lett ; 16(4): 610-24, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21909792

RESUMO

This study aimed to determine the changes in the expression of genes for selected specific transcriptional factors that have both activating and repressing functions in in vitro ductal breast cancer cells, under the influence of paclitaxel, applying the microarray technique. The cells are treated with 60 ng/ml and 300 ng/ml doses of paclitaxel that correspond to those applied in breast cancer therapy. About 60 ng/ml doses of paclitaxel cause a statistically significant increase in expression of all the 16 analysed genes coding transcriptional factors, ranging from 1.84-fold (for PO4F2) to 4.65-fold (for LMO4) (p < 0.05) in comparison with the control cells, and enhanced the taxane mechanism of action. The 300 ng/ml doses of paclitaxel cause a cytotoxic effect in the cells. In this article, we argue that these changes in gene expression values may constitute prognostic and predictive factors in ductal breast cancer therapy.


Assuntos
Biomarcadores Farmacológicos/análise , Neoplasias da Mama/tratamento farmacológico , Hidrocarbonetos Aromáticos com Pontes/farmacologia , Carcinoma Ductal de Mama/tratamento farmacológico , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Paclitaxel/farmacologia , Taxoides/farmacologia , Fatores de Transcrição , Antineoplásicos Fitogênicos/farmacologia , Antineoplásicos Fitogênicos/uso terapêutico , Apoptose/efeitos dos fármacos , Apoptose/genética , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Hidrocarbonetos Aromáticos com Pontes/uso terapêutico , Carcinoma Ductal de Mama/diagnóstico , Carcinoma Ductal de Mama/genética , Carcinoma Ductal de Mama/metabolismo , Carcinoma Ductal de Mama/patologia , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Feminino , Humanos , Análise de Sequência com Séries de Oligonucleotídeos , Paclitaxel/uso terapêutico , Cultura Primária de Células , Prognóstico , Taxoides/uso terapêutico , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Células Tumorais Cultivadas
5.
Exp Parasitol ; 128(2): 163-5, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21272584

RESUMO

It is known that highly reactive oxygene species produced during normal cellular metabolism represent a powerful effector mechanism against parasites. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) belong to the main defense anti-oxidants that prevent the formation of new free radical species. The aim of this study was to assess the activities of SOD and GPx in cattle tissues infected with Taenia saginata. We observed a statistically significant increase in the SOD and GPx activities (p=0.00003, 0.00008, respectively, Student's t-test) in skeletal muscles infected with T. saginata in spectrophotometric analysis. With the use of western blot technique, SOD synthesis stimulation has appeared in the host tissues containing cysticerci in contrast with the control samples. There was no statistically significant increase in the GPx band intensity observed in the studied samples in comparison to controls (Gene Tools Version 4.01 program). These results support the significance of anti-oxidant processes in host defense mechanism during parasitic infections.


Assuntos
Doenças dos Bovinos/enzimologia , Glutationa Peroxidase/metabolismo , Músculo Esquelético/enzimologia , Superóxido Dismutase/metabolismo , Teníase/veterinária , Animais , Western Blotting/veterinária , Estudos de Casos e Controles , Bovinos , Doenças dos Bovinos/parasitologia , Cisticercose/enzimologia , Cisticercose/parasitologia , Músculo Esquelético/parasitologia , Taenia saginata , Teníase/enzimologia
6.
Acta Parasitol ; 66(2): 677-681, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33128728

RESUMO

PURPOSE: Demodex mites infestation, typically asymptomatic, is a problem for patients with weakened immune systems because it often takes the form of symptomatic, massive infection. The Demodex mites play an important role in the occurrence of a range of eye surface diseases such as Demodex blepharitis, Meibomian gland dysfunctions, conjunctivitis and corneal changes. The ocular infection is closely related to the systemic invasion. Our goal was to minimize infestation and alleviate the symptoms of massive demodicosis so as to prevent further damage to the cornea. METHODS: Our research note involves a 61-year old woman diagnosed with secondary Sjögren syndrome due to rheumatoid arthritis. On the background of the autoimmune disease, corneal perforation of the left eye occurred that was cured by surgery. Then during the follow-up visit the patient was found (microscopically) massively infected with Demodex mites and the developed symptoms were particularly severe. RESULTS: Adequate dry eye syndrome and massive demodicosis therapy significantly reduced the number of Demodex mites and improved the patient's condition. CONCLUSION: We would like to draw the attention of the physicians of different specialties that special care should be taken with respect to the therapy of dry eye syndrome and ocular demodicosis in patients with immunological disorders to achieve therapeutic success and avoid particularly dangerous consequences of these diseases.


Assuntos
Blefarite , Pestanas , Infestações por Ácaros , Ácaros , Animais , Feminino , Humanos , Pessoa de Meia-Idade , Infestações por Ácaros/complicações
7.
Pharmacol Rep ; 71(2): 248-256, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30822618

RESUMO

BACKGROUND: Sodium dichloroacetate (DCA) is an agent with anticancer properties against solid tumors. DCA also seems to have antileukemic activity. In order to affirm it we investigate the effect of DCA on cell viability and apoptotic gene expression profiles in leukemia cell lines: CEM/C1, CCRF/CEM, HL-60, HL-60/MX2. METHODS: Cell viability was assessed by trypan blue staining. The expression of 93 genes involved in the process of apoptosis was determined by real-time PCR method using Taqman Low Density Array (TLDA). RESULTS: CEM/C1, CCRF/CEM, HL-60, HL-60/MX2 cells were exposed to DCA for 24 h. The sensitivity of each cell line to DCA is different and depends on the concentration. CEM/C1 was the most sensitive with an half-maximal inhibitory concentration (IC50) value of 30 mM, while HL-60/MX2 was the most resistant with an IC50 value of 75 mM. Exposure of leukemia cells to DCA causes differences in gene expression profiles which cannot indicate that any particular pathway of apoptosis is initiated. However, the presence of 388 statistically significant correlations between expression pattern of gens was determined. CONCLUSION: We showed that DCA causes a decrease in viability of leukemia cells. The decline depends on DCA concentration. The induction of any particular apoptosis pathway is not shown in cells after DCA treatment. For that reason, studies on the molecular mechanism of cell death after exposure to DCA should be continued.


Assuntos
Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ácido Dicloroacético/farmacologia , Leucemia/tratamento farmacológico , Antineoplásicos/administração & dosagem , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Ácido Dicloroacético/administração & dosagem , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Células HL-60 , Humanos , Concentração Inibidora 50 , Leucemia/patologia , Reação em Cadeia da Polimerase em Tempo Real
8.
Pol Merkur Lekarski ; 19(111): 291-3, 2005 Sep.
Artigo em Polonês | MEDLINE | ID: mdl-16358846

RESUMO

Many authors suggest that HPV infection can play a great role in development of benign and malignant tumours of upper respiratory tract in human. The aim of this study was to determine the prevalence of E6/E7 HPV-16 in laryngeal squamous cell carcinoma, and normal laryngeal mucosa, and to analyse their correlation with sex, lymph node status, primary tumor stage, localization, and histological differentiation. HPV 16 DNA presence was analysed using PCR technique in 72 samples of laryngeal carcinoma and in samples of 50 normal mucosa. Human papillomavirus was detected in 26 (36.1%) of the 72 patients. There was no statistically significant correlation HPV positivity and clinicopathological features of the analysed group. In 5 (10%) of 50 samples of normal mucosal. HPV 16 presence in normal mucosa and in laryngeal squamous cell carcinoma was detected in 3 patients. Our observations suggest that HPV16 can play a role in pathogenesis of laryngeal cancer.


Assuntos
Carcinoma de Células Escamosas/epidemiologia , Carcinoma de Células Escamosas/virologia , Mucosa Laríngea/virologia , Neoplasias Laríngeas/epidemiologia , Neoplasias Laríngeas/virologia , Proteínas Oncogênicas Virais/análise , Adulto , Idoso , Carcinoma de Células Escamosas/química , DNA Viral/isolamento & purificação , Feminino , Humanos , Mucosa Laríngea/química , Neoplasias Laríngeas/química , Masculino , Pessoa de Meia-Idade , Proteínas E7 de Papillomavirus , Reação em Cadeia da Polimerase , Prevalência
9.
Artigo em Inglês | MEDLINE | ID: mdl-15323196

RESUMO

Epstein-Barr virus (EBV) is a member of the herpesvirus family. The EBV genome consists of a linear DNA molecular that encoded nearly 100 viral proteins. EBV is one of the most successful viruses, infecting over 95 percent of the adult population of the world. Sera were collected from 61 patients (21 healthy patients without neoplasms, 30 cases with laryngeal squamous cell carcinoma and 10 patients with hypopharyngeal squamous cell carcinoma). The sera were titrated for EBV IgM/VCA, IgG/VCA, IgG/EBNA, IgA/VCA, IgA/EBNA and IgA/EA antibodies by enzyme linked immunosorbent assay (ELISA) technique. Only 5 (12.8%) of 39 patients with cancer were positive for anti IgM/VCA and all the controls showed negative results. 36 (92.3%) of 39 patients with cancer and 17 (94.4%) of 18 of the control group were positive for anti IgG/VCA. Seropositive anti IgG/EBNA were detected in 27 (65.8%) of 41 cases of cancer and in 11 (61.1%) of 18 healthy control group. The positive anti IgA Epstein-Barr viral capsid antigen was detected in 7 (17.9%) of 39 patients with cancer and all the control group showed negative result. No subject of the control group was positive for anti IgA Epstein-Barr early antigen and only one patient (2.56%) was positive from the group with laryngeal and hypopharyngeal cancer. 3 (7.69%) of 39 patients with cancer and 2 (11.1%) of 18 controls were positive for anti IgA Epstein-Barr nuclear antigen. The differences were not significant.


Assuntos
Anticorpos Antivirais/sangue , Herpesvirus Humano 4/imunologia , Neoplasias Hipofaríngeas/imunologia , Neoplasias Hipofaríngeas/virologia , Neoplasias Laríngeas/imunologia , Neoplasias Laríngeas/virologia , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Humanos , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Masculino , Pessoa de Meia-Idade , Neoplasias de Células Escamosas/imunologia , Neoplasias de Células Escamosas/virologia
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